Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,794	0	T	G	58.3%	4.5 / 11.6	12	G431G (GGT→GGG)	proP	proline/glycine betaine transporter
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (7/0);  total (9/3)
Fisher's exact test for biased strand distribution p-value = 4.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02

CATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCA  >  NC_000913/4331648‑4331937                                                                                                                                                   |                                                                                                                                                  cATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGGTGGtttta                                                                                                                                               >  2:278656/1‑149 (MQ=255)         ttACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGGTGGGTTTATTAcccg                                                                                                                                        >  2:365029/1‑148 (MQ=255)            cAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGGTTAAtacactgcggg                                                                                                                                    >  1:324527/1‑141 (MQ=255)                         gcggcATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAagag                                                                                                                        <  2:501299/150‑1 (MQ=255)                                                ggTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGGTGGGTTTATTACCCGCGGAACCATGAAAAAGAAGGCAAAACGTCCGGTGGAAAgg                                                                                                >  2:432135/1‑151 (MQ=255)                                                                        ggcggcCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGGTGGGTTTATCACCGGCGGAAACATGAAAAAGAAGGCAAAACGTCC‑GGTGAAAAGGGGGGCACCCGCGGCGGCAGAtata                                                                       >  2:146800/1‑151 (MQ=255)                                                                                         aaaGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAAt                                                     <  1:174357/151‑1 (MQ=255)                                                                                                    aaTCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGGTGGGTTTATTACCCGCGTAACCATGAAAGAGACGGCCAAACGTCC‑GGTGGAAAGGGGGGCACCCGCGGCGGCAGGTATACAGGAAGCG‑GAGGGAAATTTCGTCGAg                                           >  2:112187/1‑150 (MQ=255)                                                                                                     aTCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTTATCACCGGCGTAAACATGAAAGAGACGGCCAAACGTCC‑GTTGGAAGGGGCGACACCCGCGGCGTCAGATATAAAGGAAGCG‑AAGGAAAATTTCCTCGAgga                                         >  1:208337/1‑149 (MQ=255)                                                                                                                   gcctATTACCTGATGGTAGTGGCGGTGGGTGGGTTAATTACCCGCGTAACCATGAAAGAGACGGCAAATAGTCC‑GTTGGAAAGGGCGACACCCGCGGCGTCAGATATAAAGGAAACGAAAGGAAATT‑TCGTCGAGCATTAAGATAATATCg                           >  2:311002/1‑151 (MQ=255)                                                                                                                   gcctATTACCTGATGGTAGTGGCGGTGGGTGGGTTAATCACCGGCGTAAACATGAAAAAGACGGGAAAACGTCC‑GTTGGAAGGGGGGACACCCGCGGCGGCAGAAATAAAGGGAGCG‑AAAGAAAATTTTGTCGAGCATTACGATAAAATCg                           >  2:85945/1‑151 (MQ=255)                                                                                                                                           gtggtTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGAc    <  1:155008/150‑1 (MQ=255)                                                                                                                                           gtggtTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGAc    >  2:85805/1‑150 (MQ=255)                                                                                                                                             ggttggttTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCa  >  2:107715/1‑150 (MQ=255)                                                                                                                                             ggttggttTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCa  >  2:369936/1‑150 (MQ=255)                                                                                                                                             ggttggttTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCa  >  2:394227/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCC‑GTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCG‑AAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCA  >  NC_000913/4331648‑4331937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑