Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,421	C→T	L26L (TTG→TTA)	ybhN ←	UPF0104 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,421	0	C	T	100.0%	114.0 / NA	31	L26L (TTG→TTA)	ybhN	UPF0104 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

AAAAGCGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGAT  >  NC_000913/822276‑822568                                                                                                                                                  |                                                                                                                                                    aaaaGCGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAacac                                                                                                                                                <  2:121307/151‑1 (MQ=255)   aaGCGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACaa                                                                                                                                              <  1:26660/151‑1 (MQ=255)        ggCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCAc                                                                                                                                          <  2:29743/150‑1 (MQ=255)                ggTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATaaa                                                                                                                                 >  1:445984/1‑151 (MQ=255)                ggTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATaaa                                                                                                                                 >  2:500697/1‑151 (MQ=255)                                aaTCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAgg                                                                                                                 <  1:542592/151‑1 (MQ=255)                                  tCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGAt                                                                                                               <  1:150677/151‑1 (MQ=255)                                      aTAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGAtcttc                                                                                                           <  2:301545/151‑1 (MQ=255)                                      aTAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGAtct                                                                                                             <  2:33739/149‑1 (MQ=255)                                         gCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCttt                                                                                                        >  2:132761/1‑151 (MQ=255)                                           tGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCGGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGc                                                                                                      <  2:53274/151‑1 (MQ=255)                                           tGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGc                                                                                                      <  1:67674/151‑1 (MQ=255)                                           tGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGc                                                                                                      <  2:279324/151‑1 (MQ=255)                                             acgacCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTa                                                                                                    <  2:550800/151‑1 (MQ=255)                                              cgacCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTaa                                                                                                   >  1:288880/1‑151 (MQ=255)                                                                          cgcAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACtc                                                                          <  1:127859/148‑1 (MQ=255)                                                                          cgcAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACtc                                                                          <  1:122875/148‑1 (MQ=255)                                                                            cAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAg                                                                     >  2:152914/1‑151 (MQ=255)                                                                             aaCGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAgg                                                                    <  1:21020/151‑1 (MQ=255)                                                                               cgcgATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCGCCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGt                                                                  <  1:87440/151‑1 (MQ=255)                                                                                       tAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTAcccc                                                          <  2:58948/151‑1 (MQ=255)                                                                                           cgcgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCgtg                                                      >  1:218583/1‑151 (MQ=255)                                                                                           cgcgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCgtg                                                      >  1:110726/1‑151 (MQ=255)                                                                                             cgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACAC‑‑TCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGttt                                                   <  1:390445/150‑1 (MQ=255)                                                                                                   aCCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAg                                               <  1:11960/150‑1 (MQ=255)                                                                                                    ccTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGtt                                             >  2:385044/1‑151 (MQ=255)                                                                                                                ctTCCCAGTCCACTTTTTTGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATcc                                 <  1:475290/151‑1 (MQ=255)                                                                                                                              tttttGGCGTAGACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGt                   >  2:421700/1‑151 (MQ=255)                                                                                                                                         gACCACCAGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTgcgg        >  2:362877/1‑151 (MQ=255)                                                                                                                                           ccaccaGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTgcggg       >  2:501949/1‑150 (MQ=255)                                                                                                                                               caGTAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGAt  >  2:445956/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    AAAAGCGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGAT  >  NC_000913/822276‑822568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑