Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 99,276 G→C 5.9% intergenic (+70/+2) USA300HOU_RS00465 → / ← tetA_1 hypothetical protein/Tetracycline resistance protein, class C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE99,2760GC 5.9% 633.0 / 26.8 190intergenic (+70/+2)USA300HOU_RS00465/tetA_1hypothetical protein/Tetracycline resistance protein, class C
Reads supporting (aligned to +/- strand):  ref base G (95/82);  new base C (5/6);  total (102/88)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

Only 100 of 194 total aligned reads displayed.

TTCTAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTAAAAA  >  USA300TCH1516_ALE/99136‑99415
                                                                                                                                            |                                                                                                                                           
   tAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                                                                          <  1:647376/141‑1 (MQ=255)
   tAGACTTTACCGAAGAAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAATTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                                                                          <  4:112172/141‑1 (MQ=255)
    aGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCa                                                                                                                                         >  1:743235/1‑141 (MQ=255)
    aGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCa                                                                                                                                         <  1:150683/141‑1 (MQ=255)
     gACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAc                                                                                                                                        >  3:585741/1‑141 (MQ=255)
     gACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAc                                                                                                                                        >  1:433917/1‑141 (MQ=255)
      aCTTTAACGAAGCAAAAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGGAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATCGACGCTTGTTAAAGAAGAGTGACGGTCAct                                                                                                                                       <  1:592894/141‑1 (MQ=255)
        tttACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCActct                                                                                                                                     <  1:1144722/141‑1 (MQ=255)
         ttACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCACtctt                                                                                                                                    <  2:1616502/141‑1 (MQ=255)
         ttACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCACtctt                                                                                                                                    <  1:940371/141‑1 (MQ=255)
           aCCGAAGCAACAAATCTCACTGATTTATTTAAGACCATTAAAAATGATTTGAAAAAGTGAAGTAGTGAAGGGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACtcttct                                                                                                                                  >  3:552308/1‑141 (MQ=255)
           aCCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTACAGAAGGGTGACGGTCACtcttct                                                                                                                                  >  1:850327/1‑141 (MQ=255)
           aCCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACtcttct                                                                                                                                  >  2:1383193/1‑141 (MQ=255)
            ccGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTcttctt                                                                                                                                 >  1:227849/1‑141 (MQ=255)
                 gCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTAtgt                                                                                                                            >  3:264073/1‑141 (MQ=255)
                 gCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTAtgt                                                                                                                            >  1:291144/1‑141 (MQ=255)
                      aaaTCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCata                                                                                                                       >  2:1333794/1‑141 (MQ=255)
                      aaaTCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCata                                                                                                                       >  2:1091326/1‑141 (MQ=255)
                      aaaTCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCata                                                                                                                       >  1:424373/1‑141 (MQ=255)
                         tctcACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattt                                                                                                                    <  4:113287/141‑1 (MQ=255)
                                gATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTg                                                                                                             >  1:930792/1‑141 (MQ=255)
                                gATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTg                                                                                                             <  1:754059/141‑1 (MQ=255)
                                gATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTg                                                                                                             <  1:1185756/141‑1 (MQ=255)
                                 atttatttAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGc                                                                                                            <  3:707001/141‑1 (MQ=255)
                                 atttatttAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGc                                                                                                            <  1:1091326/141‑1 (MQ=255)
                                  tttatttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                                                                           <  2:1144745/141‑1 (MQ=255)
                                  tttatttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                                                                           <  4:585499/141‑1 (MQ=255)
                                  tttatttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                                                                           <  1:1539425/141‑1 (MQ=255)
                                  tttatttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                                                                           <  4:257466/141‑1 (MQ=255)
                                       ttaAGACCATAAAAAATGATATGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGttt                                                                                                      <  2:183358/141‑1 (MQ=255)
                                        taAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtttt                                                                                                     >  1:1376280/1‑141 (MQ=255)
                                        taAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtttt                                                                                                     >  1:1389642/1‑141 (MQ=255)
                                        aaaGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtttt                                                                                                     >  1:1575302/2‑141 (MQ=255)
                                         aaGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTg                                                                                                    >  2:1539167/1‑141 (MQ=255)
                                          aGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGt                                                                                                   >  1:1079578/1‑141 (MQ=255)
                                           gACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGtt                                                                                                  <  1:305544/141‑1 (MQ=255)
                                           gACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGtt                                                                                                  >  3:189569/1‑141 (MQ=255)
                                            aCCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAATAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCATGTTTTGTTa                                                                                                 >  4:381672/1‑141 (MQ=255)
                                            aCCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCATGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTa                                                                                                 >  2:1262995/1‑141 (MQ=255)
                                             ccATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTaa                                                                                                <  1:475529/141‑1 (MQ=255)
                                             ccATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGGGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTaa                                                                                                <  1:280795/141‑1 (MQ=255)
                                               aTAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACa                                                                                              >  2:1286379/1‑141 (MQ=255)
                                               aTAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTGTATGTTGCGGGTTTTGTTAACa                                                                                              >  2:280795/1‑141 (MQ=255)
                                                       tGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGcagcag                                                                                      >  3:456056/1‑141 (MQ=255)
                                                       tGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGcagcag                                                                                      <  2:857863/141‑1 (MQ=255)
                                                        gATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgt                                                                                     >  4:323916/1‑141 (MQ=255)
                                                         aTTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtg                                                                                    <  2:364863/141‑1 (MQ=255)
                                                         aTTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtg                                                                                    >  2:1080914/1‑141 (MQ=255)
                                                         aTTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtg                                                                                    >  1:1637208/1‑141 (MQ=255)
                                                          tttGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtgt                                                                                   <  4:411625/141‑1 (MQ=255)
                                                            tGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTaa                                                                                 <  2:433917/141‑1 (MQ=255)
                                                             gAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAAc                                                                                >  3:486893/1‑141 (MQ=255)
                                                                aaaGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACaaa                                                                             <  1:224480/141‑1 (MQ=255)
                                                                 aaGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAat                                                                            <  1:1211395/141‑1 (MQ=255)
                                                                  agtgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAata                                                                           >  4:302969/1‑141 (MQ=255)
                                                                  agtgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGACCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAata                                                                           >  1:1195370/1‑141 (MQ=255)
                                                                    tgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATg                                                                         <  3:34526/141‑1 (MQ=255)
                                                                      aagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAg                                                                       >  4:394131/1‑141 (MQ=255)
                                                                       agtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACaa                                                                                             >  4:287899/1‑118 (MQ=255)
                                                                         tagtgaagtGTGGGTGCAGAGAGAACTAAGCCGATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTaa                                                                    <  4:552308/141‑1 (MQ=255)
                                                                           gtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAgg                                                                  <  2:591333/141‑1 (MQ=255)
                                                                            tgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGa                                                                 <  2:904629/141‑1 (MQ=255)
                                                                             gaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGAt                                                                <  4:224419/141‑1 (MQ=255)
                                                                                gcgtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGCGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCACGGTAACAAATAGGAGTAAGGATaaa                                                             >  4:144106/3‑141 (MQ=255)
                                                                                 tgtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATaaaa                                                            >  3:246165/1‑141 (MQ=255)
                                                                                  gtgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAAt                                                           >  2:286243/1‑141 (MQ=255)
                                                                                   tgGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATg                                                          <  1:975872/141‑1 (MQ=255)
                                                                                   gggttGCATAGAGAACTAAGCCCAGCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATg                                                          <  4:490692/137‑1 (MQ=255)
                                                                                     ggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAg                                                        <  4:246791/141‑1 (MQ=255)
                                                                                     ggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAg                                                        >  1:1037642/1‑141 (MQ=255)
                                                                                       tGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGta                                                      <  3:287935/141‑1 (MQ=255)
                                                                                        gCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGtat                                                     <  2:553901/141‑1 (MQ=255)
                                                                                              agaACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAAtata                                               <  2:215851/141‑1 (MQ=255)
                                                                                                   tAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAAc                                          >  1:1173782/1‑141 (MQ=255)
                                                                                                    aaGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAAcc                                         >  3:675096/1‑141 (MQ=255)
                                                                                                     aGCCCATCGATAAATGGTCGCGTGTTAAAGAAGAGTGACGGTCACTCTTCTTGATGTGCATCTTTTATTTTGCCTGTTTTGTTAACAAGCACCACTGTAACAAATATGAGTCAGGATAAAAAGAGTATAATATAGAAACCg                                        >  4:167730/1‑141 (MQ=255)
                                                                                                      gCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGa                                       >  1:1107993/1‑141 (MQ=255)
                                                                                                         cATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTGTTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAAtt                                    <  2:877139/141‑1 (MQ=255)
                                                                                                               tAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCAt                              >  1:1066440/1‑141 (MQ=255)
                                                                                                                 aaTGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTa                            >  1:785945/1‑141 (MQ=255)
                                                                                                                   tGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAAACGAATTTATCATTAAt                          >  2:1072788/1‑141 (MQ=255)
                                                                                                                       cGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCa                      >  4:635158/1‑141 (MQ=255)
                                                                                                                          ttGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     >  2:875954/1‑91 (MQ=255)
                                                                                                                          ttGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     <  1:875954/91‑1 (MQ=255)
                                                                                                                            gTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAAt                 <  1:191900/141‑1 (MQ=255)
                                                                                                                              tAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATcc               >  1:235978/1‑141 (MQ=255)
                                                                                                                               aaaGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCa              >  1:441508/1‑141 (MQ=255)
                                                                                                                                  gaagaGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCt           <  3:42948/141‑1 (MQ=255)
                                                                                                                                  gaagaGTGACGGTCACTAATCGGGATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCt           <  1:14566/141‑1 (MQ=255)
                                                                                                                                     gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTcc        >  1:1594845/1‑141 (MQ=255)
                                                                                                                                     gagTGACGGTCACTCTTCTTGATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTcc        >  2:1583077/1‑141 (MQ=255)
                                                                                                                                      agTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCt       <  2:700798/141‑1 (MQ=255)
                                                                                                                                      agTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATAGAGACACCGAATTTATCATTAATTTCATTAATCCATCTTCCt       >  1:1605189/1‑141 (MQ=255)
                                                                                                                                      agTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGGATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCt       >  2:313389/1‑141 (MQ=255)
                                                                                                                                       gTGACAGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCAATCTTCCTa      >  2:982753/1‑141 (MQ=255)
                                                                                                                                         gACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTaaa    <  2:142466/141‑1 (MQ=255)
                                                                                                                                         gACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTaaa    >  3:132070/1‑141 (MQ=255)
                                                                                                                                           cGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTaaaaa  >  1:839617/1‑141 (MQ=255)
                                                                                                                                            ggTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAAt                                                           <  2:396451/83‑1 (MQ=255)
                                                                                                                                            ggTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAAt                                                           >  1:396451/1‑83 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTCTAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTAAAAA  >  USA300TCH1516_ALE/99136‑99415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: