BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,572	C→T	5.7%	D214D (GAC→GAT)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,572	0	C	T	5.7%	693.1 / 15.3	208	D214D (GAC→GAT)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (105/90); new base T (5/8); total (110/98)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

GCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATCT  >  USA300TCH1516_ALE/111434‑111712

gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACAt                                                                                                                                            <  2:1143904/141‑1 (MQ=255)

 cAATATATCAATTTTAAAGATATAATGAATTATAAAGATGTAGATATATCATATAATCCAGAGGTGCCGAGTTATTTAGCTCAATATCAATTAACTAATGAAGATTATAATGT‑TAAACAATTACGTAAAAGATATGACATa                                                                                                                                           >  2:686120/1‑141 (MQ=18)

 cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATa                                                                                                                                           <  1:565492/141‑1 (MQ=255)

      taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGATATAccgac                                                                                                                                      <  1:1317223/141‑4 (MQ=14)

        gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑ATAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                                    <  2:569643/141‑1 (MQ=255)

        gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                                    >  3:699214/1‑141 (MQ=255)

         cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                                                                                   >  2:1067589/1‑141 (MQ=255)

         aaaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGATATAccgacgag                                                                                                                                   <  1:626315/140‑7 (MQ=11)

          aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAAt                                                                                                                                  >  2:1607721/1‑141 (MQ=255)

             tttAAAAATTTAAAGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATTATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                               <  4:457341/141‑1 (MQ=255)

               tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                                                                             <  3:220657/141‑1 (MQ=255)

               tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAgc                                                                                                                             >  2:1153021/1‑141 (MQ=255)

               tAAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                                                                             <  2:542343/141‑1 (MQ=255)

                 aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAATTAACTAATGATGATTATAATGT‑ACAACAATTAGGTAACAGATATGACAGACCAACAAATAAAgcgc                                                                                                                           >  1:59733/1‑141 (MQ=18)

                   gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                                                                                         <  1:1373747/141‑1 (MQ=255)

                      ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                                                                                      <  4:527341/141‑1 (MQ=255)

                        aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                                                                                                    <  1:1190366/141‑1 (MQ=255)

                          tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                                  <  1:1534308/141‑1 (MQ=255)

                            aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                                                                                <  2:949821/141‑1 (MQ=255)

                             aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                                                                               >  2:402585/1‑141 (MQ=255)

                               tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                                                                             <  2:1217690/141‑1 (MQ=255)

                               tataAAGATGGAGATATATCATATAACCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                                                                             <  2:1313883/141‑1 (MQ=255)

                                 taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                                                                            >  3:458472/1‑141 (MQ=255)

                                    aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                                        >  2:1626588/1‑141 (MQ=255)

                                       tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                                     >  3:630916/1‑141 (MQ=255)

                                           gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                                 <  2:308767/141‑1 (MQ=255)

                                           gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                                 <  4:402328/141‑1 (MQ=255)

                                                  cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                                                                                          <  1:1127121/141‑1 (MQ=255)

                                                   atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                                                                                         <  1:1307372/141‑1 (MQ=255)

                                                   atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                                                                                         >  1:1049823/1‑141 (MQ=255)

                                                      taATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                                                                                      <  1:1465530/141‑1 (MQ=255)

                                                       aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                                     <  2:728147/141‑1 (MQ=255)

                                                       aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                                     >  1:1516486/1‑141 (MQ=255)

                                                        tttatgagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                                    <  2:1531214/136‑1 (MQ=255)

                                                        aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                                    <  2:443449/141‑1 (MQ=255)

                                                        aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                                    >  1:1126133/1‑141 (MQ=255)

                                                         tCCAGAGGTGCCGAGTTATTCAGCTCGATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                   <  2:1311180/141‑1 (MQ=255)

                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                   <  2:817225/141‑1 (MQ=255)

                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                   >  2:658745/1‑141 (MQ=255)

                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                   <  1:1064019/141‑1 (MQ=255)

                                                          ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                                  <  1:525849/141‑1 (MQ=255)

                                                          ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                                  <  2:159259/141‑1 (MQ=255)

                                                          ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                                  >  4:37798/1‑141 (MQ=255)

                                                            agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                                          <  4:148937/99‑1 (MQ=255)

                                                             gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca                                                                               >  2:922078/1‑141 (MQ=255)

                                                               ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                                                                             <  4:245191/141‑1 (MQ=255)

                                                                  gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                                                                          >  3:296712/1‑141 (MQ=255)

                                                                  gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                                                                          >  3:157139/1‑141 (MQ=255)

                                                                    cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                                        >  4:74488/1‑141 (MQ=255)

                                                                     gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata                                                                       <  4:616663/141‑1 (MQ=255)

                                                                     gAGTTATTCAGCTCAATATCAATTAACTAATGAGGATTATAATGT‑AAAACAATTACGTAAAATATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata                                                                       >  4:417917/1‑141 (MQ=255)

                                                                       gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata                                                                     >  3:469563/1‑141 (MQ=255)

                                                                         tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATTACATACCAACAAATAAAGTGCCGAAGCTGTTGTTGAAATGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaat                                                                   >  2:224198/1‑140 (MQ=255)

                                                                         tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa                                                                   <  3:21109/141‑1 (MQ=255)

                                                                         tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGG‑AAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                               >  1:45388/1‑81 (MQ=255)

                                                                                  cAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGaa                                                          <  4:445423/141‑1 (MQ=255)

                                                                                     tatCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATCTGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGagatc                                                       >  1:759853/1‑137 (MQ=255)

                                                                                         aaTTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                                   >  2:995460/1‑141 (MQ=255)

                                                                                         aaTTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                                   >  1:890497/1‑141 (MQ=255)

                                                                                         aaTTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                                   >  2:484312/1‑141 (MQ=255)

                                                                                         aaTTAACTAATGATGATTATAATGT‑AAAACAACTACGTAAACGTTATGACATACCAACTTATCTAGCTTCGAAGCGGTTGTTTAAATGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                                   <  3:136149/141‑1 (MQ=17)

                                                                                              aCTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                                              >  2:169164/1‑141 (MQ=255)

                                                                                              aCTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                                              >  3:444847/1‑141 (MQ=255)

                                                                                              aCTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                                              >  2:1329233/1‑141 (MQ=255)

                                                                                              aCTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                                              >  2:87231/1‑141 (MQ=255)

                                                                                               cTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTg                                                            >  4:341355/1‑126 (MQ=255)

                                                                                               cTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTg                                                            <  3:341355/126‑1 (MQ=255)

                                                                                               cTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTag                                             <  3:337342/141‑1 (MQ=255)

                                                                                               cTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTag                                             >  1:1088241/1‑141 (MQ=255)

                                                                                                tAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTaga                                            >  1:832825/1‑141 (MQ=255)

                                                                                                aaaagatgatTATAATGT‑AAAACAATTACGAAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTT‑TTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                                           <  1:363772/137‑1 (MQ=255)

                                                                                                   tgatgaTTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                                         >  4:57246/1‑141 (MQ=255)

                                                                                                   tgatgaTTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                                         >  1:397108/1‑141 (MQ=255)

                                                                                                    gatgatTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAAt                                        <  1:263673/141‑1 (MQ=255)

                                                                                                      tgatTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGAGAAGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAATTTTTTAGAGAATaa                                      >  2:908437/1‑141 (MQ=255)

                                                                                                       gatTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCGGTTGGAGATAAAAAAATTGAATTTACTTTTTTAGAGAATaaa                                     <  1:1423025/141‑1 (MQ=255)

                                                                                                       gatTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaa                                     >  4:489820/1‑141 (MQ=255)

                                                                                                         ttATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGATATTaaata                                   >  2:978785/1‑139 (MQ=255)

                                                                                                          tataATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAAt                                  <  4:294124/141‑1 (MQ=255)

                                                                                                          tataATGT‑AAAACAAGTACGTAAACGATATGACATACCAACAAATAAAGCGCCGAAGCTGCTGTTGAAAGGTACAGGGAATATAGAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAAt                                  >  2:1444027/1‑141 (MQ=18)

                                                                                                           ataATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATg                                 <  3:389812/141‑1 (MQ=255)

                                                                                                           ataATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATg                                 >  1:1038937/1‑141 (MQ=255)

                                                                                                            taATGT‑AAAACAATTCCGTAAACGATACGACCTACCGACCGATAAAGGGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCTTCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGa                                <  1:1153021/141‑1 (MQ=18)

                                                                                                               tGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaaa                             <  2:887189/141‑1 (MQ=255)

                                                                                                                   aaaaCAATTACGTAAAAGATATGCCATACCAACAAAAAAAGAGCCCAAGCAGTAGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAatat                          >  2:59127/1‑141 (MQ=18)

                                                                                                                   aaaaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAatat                          >  1:311512/1‑141 (MQ=255)

                                                                                                                      aaaaTTACGTAAAAGATATGACATAACAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta                       <  2:1004885/139‑1 (MQ=255)

                                                                                                                       cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                                 <  1:1608582/66‑1 (MQ=255)

                                                                                                                       cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac                      >  1:18051/1‑141 (MQ=255)

                                                                                                                        aaTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttact                     <  2:1054584/141‑1 (MQ=255)

                                                                                                                          ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTAAAAATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt                   <  2:461642/141‑1 (MQ=255)

                                                                                                                             cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact                >  2:135300/1‑141 (MQ=255)

                                                                                                                               tAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGa              >  4:173164/1‑141 (MQ=255)

                                                                                                                                 aaaGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTg                                                            <  3:266115/93‑1 (MQ=255)

                                                                                                                                 aaaGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATa            >  1:696593/1‑141 (MQ=255)

                                                                                                                                 aaaGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATa            >  4:56556/1‑141 (MQ=255)

                                                                                                                                 aaaGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATa            >  4:640534/1‑141 (MQ=255)

                                                                                                                                     atatGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCt        <  2:698613/141‑1 (MQ=255)

                                                                                                                                       atGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTAc      <  3:518099/141‑1 (MQ=255)

                                                                                                                                           cATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATCt  >  1:683306/1‑141 (MQ=255)

GCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATCT  >  USA300TCH1516_ALE/111434‑111712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`