Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,558 T→C 5.6% S1220S (AGT→AGC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5580TC 5.6% 533.6 / 6.6 193S1220S (AGT→AGCsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (88/91);  new base C (8/5);  total (97/96)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

TCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGA  >  USA300TCH1516_ALE/618418‑618698
                                                                                                                                            |                                                                                                                                            
tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGt                                                                                                                                              >  1:1226049/1‑141 (MQ=255)
  aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGa                                                                                                                                            <  2:931062/141‑1 (MQ=255)
   gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGtagc                                                                                                                                           >  2:1560668/1‑138 (MQ=255)
      tCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtca                                                                                                                                        <  1:1365334/141‑1 (MQ=255)
       cAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACtcag                                                                                                                                       <  1:1311212/141‑1 (MQ=255)
        aGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGAGACTCAGCCCCACATAGTggtaatga                                                                                                                                      >  4:382879/1‑134 (MQ=14)
         gATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                                                     <  1:202540/141‑1 (MQ=11)
           tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                                                                                                   >  1:573704/1‑141 (MQ=255)
             gcgactcagattcagacagcgactcagattcagacaGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACtcagattcag                                                                                                                                 >  4:413744/1‑141 (MQ=255)
                 ctcagattcagacagcgactcagattcagacagcgaCTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAg                                                                                                                             <  4:689569/141‑1 (MQ=255)
                    agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGa                                                                                                                          <  1:901297/141‑1 (MQ=255)
                    agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGa                                                                                                                          <  4:405866/141‑1 (MQ=255)
                    agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACACAGATAGCGACTCAGATTCAGATAGCGa                                                                                                                          >  2:1614464/1‑141 (MQ=255)
                     gattcagacagcgactcagattcagacagcgactcaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAc                                                                                                                         <  3:209533/141‑1 (MQ=255)
                      attcagacagcgactcagattcagacagcgactcagACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACt                                                                                                                        <  2:1083643/141‑1 (MQ=255)
                         cagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAg                                                                                                                     >  2:1573561/1‑141 (MQ=255)
                           gacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGAt                                                                                                                   <  1:1006133/141‑1 (MQ=255)
                                cgactcagattcagacagcgactcagaCTCAGCTAGTGATTCAGACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                              <  1:1562258/141‑1 (MQ=255)
                                cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                              <  1:1537529/141‑1 (MQ=255)
                                cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                              <  1:639933/141‑1 (MQ=255)
                                cgactcagattcagacagcgacacagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                              <  2:398300/141‑1 (MQ=255)
                                   ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                           <  3:543251/141‑1 (MQ=255)
                                      agattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                        <  4:553048/141‑1 (MQ=255)
                                            agccagcgagtcagaTTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                  <  1:1213543/138‑1 (MQ=255)
                                            agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                  >  1:347589/1‑141 (MQ=255)
                                            agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                  <  3:355764/141‑1 (MQ=255)
                                              acagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACt                                                                                                >  2:681333/1‑141 (MQ=255)
                                               cagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACCCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                                                               <  1:1292414/141‑1 (MQ=255)
                                               cagcgactcagaCTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                                                               <  1:494765/141‑1 (MQ=255)
                                               cagcgactcagaCGCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTGAGATTCAGATAGCGACTCAGATTCGAATAGCGATTCAGACTc                                                                                               >  1:435636/1‑141 (MQ=255)
                                                  cgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                                                            <  1:681333/141‑1 (MQ=255)
                                                     ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGAATCAGATAGCGACCGAGATTCAGAAAGCGATTCAGACTCAGATAg                                                                                         >  2:231477/1‑141 (MQ=14)
                                                      tcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACACAGACACAGACAGCGATTCAGACACAGATAgg                                                                                        >  2:1546808/1‑140 (MQ=11)
                                                       cagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACCCAGATAGCg                                                                                       >  2:1000536/1‑141 (MQ=255)
                                                         gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                     <  1:603991/141‑1 (MQ=255)
                                                         gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                     <  1:735963/141‑1 (MQ=255)
                                                         gactcagaTAGCGATCCAGACTCAGATAGCGGCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                     <  2:844641/141‑1 (MQ=255)
                                                          actcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACt                                                                                    <  1:465424/141‑1 (MQ=255)
                                                          actcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACt                                                                                    >  1:1200418/1‑141 (MQ=255)
                                                             cagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag                                                                                 >  1:637769/1‑141 (MQ=255)
                                                              agaTAGTGATTCAGACTCAGATAGCGACTCAGATGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                >  1:1486447/1‑141 (MQ=255)
                                                               gaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAACGACTCGGACGCAGATAGCGACTCAGACCCAGATAGCGACgcagat                                                                               >  2:456167/1‑141 (MQ=11)
                                                                 tagcgactcagattcagatAGTGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattc                                                                             >  2:740387/8‑141 (MQ=11)
                                                                  aGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattca                                                                            >  3:317060/1‑141 (MQ=255)
                                                                    tGATTCTGACTCAGATAGCGACTCAGATGTAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                          <  2:1147153/141‑1 (MQ=255)
                                                                    tGATTCAGTCTCAGTTAGCGACTCAGTTTCAGAATTCGACTTAACATCTGACCGCGACTCAGACCCAGATAGTGACTCAGATGCAGATAGTGACTCAGATTCGGACAGCGATACAGACTCAGATAGCGACtcagattcaga                                                                          <  2:1312974/141‑1 (MQ=17)
                                                                     gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                         >  1:723230/1‑141 (MQ=255)
                                                                       ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATACAGATAg                                                                       >  2:1656317/1‑141 (MQ=14)
                                                                       ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                       <  2:438968/141‑1 (MQ=255)
                                                                           gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                   >  1:1177319/1‑141 (MQ=255)
                                                                            aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCg                                                                                                                           <  3:147953/84‑1 (MQ=255)
                                                                            aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCg                                                                                                                           >  4:147953/1‑84 (MQ=255)
                                                                            aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAGTCAGACTCAGATAGCGACTCAGCTTCAGATAGCGCtt                                                                  >  1:83020/1‑141 (MQ=255)
                                                                               cAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCgg                                                               >  2:1597899/1‑141 (MQ=255)
                                                                                 gATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGAc                                                             <  4:119813/141‑1 (MQ=255)
                                                                                 gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                             <  3:8920/141‑1 (MQ=255)
                                                                                  aTAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACt                                                            >  4:584193/1‑141 (MQ=255)
                                                                                  aTAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACt                                                            >  4:306614/1‑141 (MQ=255)
                                                                                    aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                    >  2:1173690/1‑131 (MQ=255)
                                                                                    aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                    <  1:1173690/131‑1 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                    <  1:1482777/128‑1 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                    >  2:1482777/1‑128 (MQ=255)
                                                                                         cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACCCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATGCGGACGGCGATTGAGACACAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAg                                                     >  2:697539/1‑141 (MQ=17)
                                                                                           cAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc                                                                 >  1:1383698/1‑127 (MQ=255)
                                                                                           cAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc                                                                 <  2:1383698/127‑1 (MQ=255)
                                                                                            aGATTCAGAGAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                  <  4:514586/141‑1 (MQ=255)
                                                                                            aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                  <  1:88240/141‑1 (MQ=255)
                                                                                            aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGa                                                  >  1:1114303/1‑141 (MQ=255)
                                                                                               ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                               <  1:1391985/141‑1 (MQ=255)
                                                                                                  agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                            <  2:525190/141‑1 (MQ=255)
                                                                                                     cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                         >  1:1418071/1‑141 (MQ=255)
                                                                                                     cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                         >  1:1079967/1‑141 (MQ=255)
                                                                                                           ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                   <  3:671766/141‑1 (MQ=255)
                                                                                                           ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                   >  1:1617569/1‑141 (MQ=255)
                                                                                                           ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                   >  2:371072/1‑141 (MQ=255)
                                                                                                           ctcagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAAATACAGATac                                   >  2:50237/1‑140 (MQ=255)
                                                                                                             cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                 >  1:1240095/1‑141 (MQ=255)
                                                                                                             cagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAgcg                                 >  4:483708/1‑139 (MQ=255)
                                                                                                              aggctcagacagcgactcaggttcagaTAGCGACTCAGAATCAGATAGCGATTCGGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                <  1:1028278/138‑1 (MQ=11)
                                                                                                              agactcagacagcgactcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                <  3:426411/141‑1 (MQ=255)
                                                                                                              agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                <  2:79504/141‑1 (MQ=255)
                                                                                                               gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                               >  1:1608020/1‑141 (MQ=255)
                                                                                                               gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGAAAGCGATTGGGACTCAGATAGAGACTCAGATTCAGATAGTGAt                               >  2:226140/1‑141 (MQ=17)
                                                                                                                  tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                            >  1:200686/1‑141 (MQ=255)
                                                                                                                  tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                            <  1:1425212/141‑1 (MQ=255)
                                                                                                                    agacagcgactcagactcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                          <  2:1004730/141‑1 (MQ=255)
                                                                                                                       ctgcgactgagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                       <  2:28235/139‑1 (MQ=14)
                                                                                                                       cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTGAGACTCAGATAGCGAATCAGAGGCAGATAGCGAGACAGACTCAGATAGCGACTCAGATGCAGATAGTGATACAGACTc                       >  2:880342/1‑141 (MQ=17)
                                                                                                                       cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                       <  3:489764/141‑1 (MQ=255)
                                                                                                                          cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                    <  1:257616/141‑1 (MQ=255)
                                                                                                                          cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                    <  3:456746/141‑1 (MQ=255)
                                                                                                                          cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                    >  1:1000683/1‑141 (MQ=255)
                                                                                                                           gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGAt                   >  1:91714/1‑141 (MQ=255)
                                                                                                                           gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACCCACATAGCGTATCAGAGACCGATAGCGATTCGGATACACATAGCGCCTCAGATCCAGATAGTGATTCAGACTCAGAt                   <  2:1226049/141‑1 (MQ=17)
                                                                                                                            actcagactcagaTAGTGACTCAGAATCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                  <  2:484879/141‑1 (MQ=255)
                                                                                                                             ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                 >  2:1414064/1‑141 (MQ=255)
                                                                                                                                 gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGGCTCAGATAGCGAc             >  3:102199/1‑141 (MQ=255)
                                                                                                                                   ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc           <  2:82373/141‑1 (MQ=255)
                                                                                                                                    tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa          >  1:617808/1‑141 (MQ=255)
                                                                                                                                    tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa          >  2:1513197/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGA  >  USA300TCH1516_ALE/618418‑618698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: