Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,588 G→A 7.9% S1230S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5880GA 7.9% 553.7 / 3.6 203S1230S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (95/86);  new base A (13/8);  total (109/94)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 206 total aligned reads displayed.

GCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGA  >  USA300TCH1516_ALE/618449‑618728
                                                                                                                                           |                                                                                                                                            
 cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                                                            <  1:639933/141‑1 (MQ=255)
  gactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGAc                                                                                                                                           <  3:52113/141‑1 (MQ=255)
       agattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                                                      <  4:553048/141‑1 (MQ=255)
             agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                                                >  1:347589/1‑141 (MQ=255)
              gacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGATAGTGACTCAGATTCAAATAGCGACTCAGATTCAGACAGCGATGTAGAc                                                                                                                               >  1:1385717/1‑141 (MQ=14)
               acagcgactcagaCTCAGATAGTGATTCAGACTCAGATAACGACTCAGATTCAGACAGCGACACAGACGCAGACAGCGCCTCAGACGTAGATAGTGATTCGCATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACt                                                                                                                              >  1:1613537/1‑141 (MQ=14)
                cagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCACATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTAAGATTCAGATAGCAACTCAGATTCGGACAGCGATTCAGACTc                                                                                                                             >  2:157298/1‑141 (MQ=255)
                   cgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                                                                                          <  1:681333/141‑1 (MQ=255)
                      ctctgtctcataTAGTGATTCAGACGCAGATAACAACTCAGTTTCAGACAGCTACTCAGACGCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                                       <  3:139580/135‑1 (MQ=14)
                      ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                                       >  2:45242/1‑141 (MQ=255)
                      ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                                       <  2:688948/141‑1 (MQ=255)
                       tcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACACAGACACAGACAGCGATTCAGACACAGATAgg                                                                                                                      >  2:1546808/1‑140 (MQ=11)
                       gcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCATACAGCGACTCAGACTCAGAGATTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGc                                                                                                                      >  1:1569811/2‑141 (MQ=255)
                        cagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACCCAGATAGCg                                                                                                                     >  2:1000536/1‑141 (MQ=255)
                         agcctcagaTACTGCTTCAGACTCCGATAGAGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGACAGCGACTCAGATTCGGACAGCGATTCAGGCTCAGATAGCGa                                                                                                                    <  2:1654133/138‑1 (MQ=14)
                          gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                                                   >  4:456746/1‑141 (MQ=255)
                          gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                                                   <  1:735963/141‑1 (MQ=255)
                          gactcagaTAGCGATCCAGACTCAGATAGCGGCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                                                   <  2:844641/141‑1 (MQ=255)
                            ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGATTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                                                 <  1:879913/141‑1 (MQ=255)
                             tcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca                                                                                                                <  1:1369682/141‑1 (MQ=255)
                              cagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag                                                                                                               >  1:637769/1‑141 (MQ=255)
                               agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                                              <  1:1560668/141‑1 (MQ=255)
                                 aTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAATCAGACTCAGATAGCGATtcaaacc                                                                                                            >  2:1151980/1‑137 (MQ=255)
                                    gTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGAGAAtcagattcag                                                                                                         >  2:398975/1‑141 (MQ=14)
                                     tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                                        <  2:728236/141‑1 (MQ=255)
                                      gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                                                       >  1:723230/1‑141 (MQ=255)
                                        ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCCGATTCAGATAGCGACACAGAACCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                     >  1:1238723/1‑141 (MQ=255)
                                        ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                     <  1:1037488/141‑1 (MQ=255)
                                            gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                                                 >  1:1177319/1‑141 (MQ=255)
                                             aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAGTCAGACTCAGATAGCGACTCAGCTTCAGATAGCGCtt                                                                                                >  1:83020/1‑141 (MQ=255)
                                              cTCAGATCGCGATCCAGATTCAGTCAGCGACTCAGCCTCAGGCAGCGACTCAGCCTCAGCTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc                                                                                               <  4:103891/141‑1 (MQ=14)
                                                cAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCgg                                                                                             >  2:1597899/1‑141 (MQ=255)
                                                     aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                                                  <  1:1173690/131‑1 (MQ=255)
                                                       cGACTCCGATTCCGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                      <  2:1511228/141‑1 (MQ=255)
                                                       cGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                      <  3:657001/141‑1 (MQ=255)
                                                        gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                                                  >  2:1482777/1‑128 (MQ=255)
                                                          cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                   <  1:474699/141‑1 (MQ=255)
                                                             aGATTCAGAGAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                <  4:514586/141‑1 (MQ=255)
                                                             aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGa                                                                                >  1:1114303/1‑141 (MQ=255)
                                                                ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACtc                                                                             >  4:213397/1‑141 (MQ=255)
                                                                 tcagacagcgactcagactcagacagcgactcagacTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtca                                                                            >  1:129413/1‑141 (MQ=255)
                                                                    gacagtgactcagactcagacagcgactcagactcaGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGAATCAGACACAGATAGCGAATCAGATCCAAATAGAGATACGCACGCAGATGGCGAGtcagag                                                                         >  2:1367487/1‑140 (MQ=11)
                                                                    gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTTAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                         >  1:202379/1‑141 (MQ=255)
                                                                    gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                         >  1:1290549/1‑141 (MQ=255)
                                                                     acagcgactcagactcagacagcgactcagtctcagATAGTGACTCAGATGCAGAGAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagatt                                                                        <  3:676299/141‑1 (MQ=255)
                                                                      cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                       >  1:1079967/1‑141 (MQ=255)
                                                                         cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                    >  1:602814/1‑141 (MQ=255)
                                                                            ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                 >  1:1617569/1‑141 (MQ=255)
                                                                            ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                 >  3:361421/1‑141 (MQ=255)
                                                                            ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                 >  2:371072/1‑141 (MQ=255)
                                                                              cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                                               >  1:61038/1‑141 (MQ=255)
                                                                              cagactcagacagcgactcagacacagaTAGTGACCCAGACACAGATAGCGACTCAGATCCCGACAGCGAATCAGACTCAGATAGCGACCCAGATTCAGACAGCGATTCAGACTCAGATAGCGACCCAGAATCAGATAGTg                                                               >  2:1099601/1‑141 (MQ=14)
                                                                               agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                              <  2:79504/141‑1 (MQ=255)
                                                                               agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                              <  4:570216/141‑1 (MQ=255)
                                                                                   tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                                          <  1:1425212/141‑1 (MQ=255)
                                                                                    cagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAg                                                         >  2:746827/1‑141 (MQ=255)
                                                                                     agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                        >  2:228136/1‑141 (MQ=255)
                                                                                     agacagcgactcagactcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                        <  2:1004730/141‑1 (MQ=255)
                                                                                      gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                                                       >  1:1400498/1‑141 (MQ=255)
                                                                                        ctgcgactgagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                     <  2:28235/139‑1 (MQ=14)
                                                                                        cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                     <  3:489764/141‑1 (MQ=255)
                                                                                        cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGATAGTGATTCAGACTc                                                     >  1:179573/1‑141 (MQ=255)
                                                                                           cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATATCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                  <  1:1213417/141‑1 (MQ=255)
                                                                                           cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                  <  3:456746/141‑1 (MQ=255)
                                                                                           cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                  >  1:1000683/1‑141 (MQ=255)
                                                                                           cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                  <  1:257616/141‑1 (MQ=255)
                                                                                            gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGAt                                                 >  1:152813/1‑141 (MQ=255)
                                                                                            gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGAt                                                 >  1:91714/1‑141 (MQ=255)
                                                                                            gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACCCACATAGCGTATCAGAGACCGATAGCGATTCGGATACACATAGCGCCTCAGATCCAGATAGTGATTCAGACTCAGAt                                                 <  2:1226049/141‑1 (MQ=17)
                                                                                            gactcagactcagaTAGTGACTCAGATTCAGATAGAGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGAt                                                 <  4:372146/141‑1 (MQ=255)
                                                                                             actcagactcagaTAGTGACTCAGAATCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                                                <  2:484879/141‑1 (MQ=255)
                                                                                              ctcagactcagaTGGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                               <  2:644100/141‑1 (MQ=255)
                                                                                              ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                               >  2:1414064/1‑141 (MQ=255)
                                                                                                  gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGAGGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                           >  1:44493/1‑141 (MQ=255)
                                                                                                    ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                         <  2:82373/141‑1 (MQ=255)
                                                                                                        gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAt                                     >  3:337317/1‑141 (MQ=255)
                                                                                                        gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGac                                     >  2:90425/1‑140 (MQ=255)
                                                                                                          tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTc                                   >  3:215082/1‑141 (MQ=255)
                                                                                                           aGTGACACAGATTCAGATACCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                  <  2:519944/141‑1 (MQ=255)
                                                                                                              gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                               <  1:388154/141‑1 (MQ=255)
                                                                                                                cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                            <  2:1064836/110‑1 (MQ=255)
                                                                                                                cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                             >  2:1278388/1‑141 (MQ=255)
                                                                                                                     attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                        <  1:126284/141‑1 (MQ=255)
                                                                                                                      ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc                       >  1:1459692/1‑141 (MQ=255)
                                                                                                                      ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGATAGTGATGCAGACTCAGATAGCGAATCAGATTCAGACAGCGACTc                       >  1:897818/1‑141 (MQ=255)
                                                                                                                      taccaatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc                       <  2:1521064/136‑1 (MQ=255)
                                                                                                                       tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCa                      <  1:267203/141‑1 (MQ=255)
                                                                                                                        cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                     >  4:298797/1‑141 (MQ=255)
                                                                                                                        cagacAGCGACTCAGAATCAGATAGCGATTCAGAATCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                     >  3:192396/1‑141 (MQ=14)
                                                                                                                         agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGa                    >  4:587980/1‑141 (MQ=255)
                                                                                                                         agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGa                    >  2:471082/1‑141 (MQ=255)
                                                                                                                         agatAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGa                    <  4:123616/141‑1 (MQ=255)
                                                                                                                          gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCCGACTCAGATAACGACACAGATTCAGAAAGAGACTCAGaa                   >  2:22176/1‑140 (MQ=14)
                                                                                                                          gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                        >  2:504633/1‑120 (MQ=255)
                                                                                                                          gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                        <  1:504633/120‑1 (MQ=255)
                                                                                                                          gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc                         >  1:1018032/1‑135 (MQ=255)
                                                                                                                                   tCAGATACGGCAAGCGATTCAGACTCAGATGGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc          <  4:403196/141‑1 (MQ=17)
                                                                                                                                         tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCa    >  3:1139/1‑141 (MQ=255)
                                                                                                                                          cGGACAGCGATTCAGACTCAGATAGCGACTCCGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAg   >  3:26649/1‑141 (MQ=255)
                                                                                                                                          aggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAg   >  1:1574046/2‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGA  >  USA300TCH1516_ALE/618449‑618728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: