Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,545 T→C 5.7% D1036D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5450TC 5.7% 516.6 / 2.1 180D1036D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (92/74);  new base C (7/6);  total (100/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

GACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGA  >  USA300TCH1516_ALE/622405‑622682
                                                                                                                                            |                                                                                                                                         
gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                                           >  1:205291/1‑141 (MQ=255)
  cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                                         <  4:672052/141‑1 (MQ=255)
  cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAg                                                                                                                                         >  2:1328877/1‑141 (MQ=255)
  cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGAGTCGGACTCagagag                                                                                                                                         >  2:1157655/1‑141 (MQ=255)
   tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                                                                                        <  4:317272/141‑1 (MQ=255)
    cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                                       >  1:103034/1‑141 (MQ=255)
    cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCg                                                                                                                                       >  2:1271246/1‑141 (MQ=255)
     agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                                      <  2:1357314/141‑1 (MQ=255)
        ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                                                                                   <  2:1566639/141‑1 (MQ=255)
        ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                                                                                   >  1:1204396/1‑141 (MQ=255)
         tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                                                                                  >  1:870425/1‑141 (MQ=255)
          cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                                                 <  1:705186/141‑1 (MQ=255)
          cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                                                 <  1:705145/141‑1 (MQ=255)
          cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAg                                                                                                                                 >  2:887717/1‑141 (MQ=255)
          cagatAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCGGACTCAGACAGCGACTCAGATTCAGATAGCGACTGGGACTCAGATAGCGACTCAg                                                                                                                                 >  2:116766/1‑141 (MQ=14)
               aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGACACAGACAGCGACTCAGATCCAGAGAGCGACTCGGACTCAGACAGCGACTCAGacaca                                                                                                                            >  2:297258/1‑137 (MQ=14)
                 cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                                          <  2:227955/141‑1 (MQ=255)
                      cagactcagacAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATACAGACTCAGAACCCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGAGATTCAGACACAGGCAGCGACTCAGATTCCGACAGCg                                                                                                                     >  3:837/1‑141 (MQ=11)
                       agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                                                    >  1:76665/1‑141 (MQ=255)
                        gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGACTCGGACGCAGACGGCGACTCAGCGTCAGAGAGCGaa                                                                                                                   >  2:128767/1‑140 (MQ=11)
                         attcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                                  >  2:804295/3‑141 (MQ=255)
                         actcagatAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATACCCATTCGGACTCAGATCGACACTCAGATTCAAACACCGact                                                                                                                  >  4:61235/1‑139 (MQ=11)
                          ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                                 <  3:51765/141‑1 (MQ=255)
                            cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                               >  1:529375/1‑141 (MQ=255)
                            cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                               >  1:934688/1‑141 (MQ=255)
                              gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                                             <  1:1079048/141‑1 (MQ=255)
                                caccgccTCCGATTCAGACAGCGACTCGGACTCAGACAGCGATTCAGACTCAGACAGCGCCTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                           <  2:874856/135‑1 (MQ=14)
                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                           >  1:791593/1‑141 (MQ=255)
                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                           >  3:565602/1‑141 (MQ=255)
                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                           <  2:730677/141‑1 (MQ=255)
                                  gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                                         >  2:266033/1‑141 (MQ=255)
                                    cactcagactcagACAGCGCGTCGGACTCAGACTCTGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                                       <  2:1185904/140‑1 (MQ=14)
                                      cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                                     >  1:1321208/1‑141 (MQ=255)
                                          gattcagatACCGACGCGGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                 <  4:388076/141‑1 (MQ=14)
                                          gattcagacAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                 <  2:407160/141‑1 (MQ=255)
                                            ttcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                               <  2:791593/141‑1 (MQ=255)
                                               agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                                                            <  1:1119414/141‑1 (MQ=255)
                                                  tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGCTTCGGAGTCAGTTAGCGGCTCAGATTCAGACAGCGGTTCAGACTCAGATAGCgactcagcctc                                                                                         >  3:203139/1‑141 (MQ=17)
                                                  tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcagactc                                                                                         >  1:1648897/1‑141 (MQ=255)
                                                  caGCGATTGTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                         <  4:575941/140‑1 (MQ=255)
                                                    gtgaggcGGACTCACACACCGCCTCAGACGCAGAGAGCGACTCAGACTCAGACAGCGACTAAGAGTCAGACAGCGACTCAGAATCAGATAGAGATACAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                       <  4:630924/135‑1 (MQ=11)
                                                    gCGATGCGGACGCAGAAAGCGCCTAAGACTCAGATATCGACGCAGACTCAGACAGCGACTCAGATTCAGAAAGCGACTCGGACTCAGACAGCGACCCAGAGTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                       <  4:417747/141‑1 (MQ=14)
                                                      gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                     <  1:515326/141‑1 (MQ=255)
                                                       aTTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcagata                                                                                    <  4:561087/141‑3 (MQ=255)
                                                          cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                                 >  3:446370/1‑141 (MQ=255)
                                                           ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                                <  1:1600242/141‑1 (MQ=255)
                                                           ggACTCAGACAGCGATTCAGACGCAGATAGCGCCTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                                <  4:469497/141‑1 (MQ=255)
                                                             aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt                                                                              >  2:1032420/1‑141 (MQ=255)
                                                              cTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattc                                                                             >  3:122724/1‑141 (MQ=255)
                                                                cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTAAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag                                                                           >  1:1436353/1‑141 (MQ=255)
                                                                 aGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                                          >  2:811447/1‑141 (MQ=255)
                                                                  gACAGCGATTCCGACTCAGATAGCGACTCAGCCTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat                                                                         <  4:382740/141‑1 (MQ=255)
                                                                  gACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGACGGCgaatcagaa                                                                         >  2:224717/1‑140 (MQ=11)
                                                                  gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAGATAGCGACTCCGACTCAGACAGTgagtcagat                                                                         >  2:279239/1‑141 (MQ=255)
                                                                    cAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                                       <  2:775002/141‑1 (MQ=255)
                                                                      gCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagactgcg                                                                     >  2:399716/1‑136 (MQ=255)
                                                                       cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga                                                                    <  1:889790/141‑1 (MQ=255)
                                                                            cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCg                                                               >  1:1512543/1‑141 (MQ=255)
                                                                              gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGATAGCgaa                                                             >  4:269332/1‑140 (MQ=255)
                                                                                cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                           >  2:713930/1‑141 (MQ=255)
                                                                                cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                           >  1:639045/1‑141 (MQ=255)
                                                                                   aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcaga                                                        >  2:1555967/1‑141 (MQ=255)
                                                                                   aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcaga                                                        >  1:1158503/1‑141 (MQ=255)
                                                                                    gATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAGTCAGACAGCgactcagac                                                       >  4:306758/1‑141 (MQ=255)
                                                                                       aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactca                                                    >  2:867328/1‑141 (MQ=255)
                                                                                       aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactca                                                    <  2:139280/141‑1 (MQ=255)
                                                                                          gactcagactcaGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGATTCAGACAGCGACTCAGACACAGAt                                                 >  3:628515/1‑141 (MQ=255)
                                                                                            ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAg                                               >  4:561670/1‑141 (MQ=255)
                                                                                             tcagactcagacAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGc                                              >  2:91880/1‑141 (MQ=255)
                                                                                              cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATaacg                                             >  1:916821/1‑138 (MQ=255)
                                                                                                 actcagacAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACt                                          <  4:533808/141‑1 (MQ=255)
                                                                                                  ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTc                                         >  1:353762/1‑141 (MQ=255)
                                                                                                     agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGa                                      <  1:253515/141‑1 (MQ=255)
                                                                                                        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCga                                                              >  2:1621433/1‑114 (MQ=255)
                                                                                                        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                                   >  1:376613/1‑141 (MQ=255)
                                                                                                          gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                                 >  4:431214/1‑141 (MQ=255)
                                                                                                          gCGACTCAGATTCAGATAGAGATTCGGACTCAGATAGCGACTCAGAATCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                                 <  3:169527/141‑1 (MQ=255)
                                                                                                           cgactccgactcAGATAGCGATTCGGACTCAGATAGCGACTCAGACGCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                                <  2:134269/141‑1 (MQ=17)
                                                                                                           cGACTCCGATTCAGAAAGCGACTCAGACTCAGACAGCGACTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                                <  2:1409291/141‑1 (MQ=14)
                                                                                                            gACTCAGATTTAGATAGCGATTCGGACTCTGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTTAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                               <  3:611095/141‑1 (MQ=255)
                                                                                                            gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                               >  1:1069673/1‑141 (MQ=255)
                                                                                                            gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                               >  3:459858/1‑141 (MQ=255)
                                                                                                              cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAg                             >  4:640570/1‑141 (MQ=255)
                                                                                                              cTCAGATGCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACacagacag                             >  2:1151825/1‑141 (MQ=255)
                                                                                                                cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCg                           >  1:1174582/1‑141 (MQ=255)
                                                                                                                  gattcatatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGAc                         <  1:62728/141‑1 (MQ=255)
                                                                                                                       agatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCTGACAGCGACTCAGa                    <  3:308087/141‑1 (MQ=255)
                                                                                                                        gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGAc                   >  3:310860/1‑141 (MQ=255)
                                                                                                                          tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGAGacacacacaa                 >  2:486915/1‑139 (MQ=17)
                                                                                                                            gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg               >  1:841025/1‑141 (MQ=255)
                                                                                                                            gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg               >  2:625099/1‑141 (MQ=255)
                                                                                                                            gCGACTCCGACTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg               <  2:752556/141‑1 (MQ=14)
                                                                                                                              gataccgACTCCGACAGCGACACAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGAt             <  2:508104/135‑1 (MQ=14)
                                                                                                                                ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTTATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg           >  1:30226/1‑141 (MQ=255)
                                                                                                                                ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg           <  1:96345/141‑1 (MQ=255)
                                                                                                                                 tCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGc          <  1:1230968/141‑1 (MQ=255)
                                                                                                                                  cGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCg         >  1:280212/1‑141 (MQ=255)
                                                                                                                                    gACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgac       <  1:423743/141‑1 (MQ=255)
                                                                                                                                        cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcag   >  2:457604/1‑141 (MQ=255)
                                                                                                                                            tAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                                          >  1:1630142/1‑66 (MQ=255)
                                                                                                                                            |                                                                                                                                         
GACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGA  >  USA300TCH1516_ALE/622405‑622682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑