BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,333,796	T→G	9.8%	V66G (GTT→GGT)	hflX →	GTPase HflX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,333,796	0	T	G	9.8%	569.6 / 9.6	192	V66G (GTT→GGT)	hflX	GTPase HflX
Reads supporting (aligned to +/- strand): ref base T (79/91); new base G (20/2); total (99/93)
Fisher's exact test for biased strand distribution p-value = 5.85e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

GGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGAACT  >  USA300TCH1516_ALE/1333657‑1333935

ggTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAAGATTATGtt                                                                                                                                            >  4:331153/1‑141 (MQ=255)

   gtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggt                                                                                                                                         >  2:129388/1‑141 (MQ=255)

      cATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggcaaa                                                                                                                                      >  2:687792/1‑141 (MQ=255)

       aTGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaag                                                                                                                                     >  3:132518/1‑141 (MQ=255)

       aTGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaag                                                                                                                                     >  4:276483/1‑141 (MQ=255)

         gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                   <  2:897852/141‑1 (MQ=255)

         gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                   <  1:1159493/141‑1 (MQ=255)

         gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                   <  2:78714/141‑1 (MQ=255)

            cAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggtaaa                                                                                                                                <  1:467545/141‑1 (MQ=255)

               gatgatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAAtt                                                                                                                             >  2:593136/1‑141 (MQ=255)

                atgatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTg                                                                                                                            <  2:1233920/141‑1 (MQ=255)

                  gatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaa                                                                                                                          >  4:223160/1‑141 (MQ=255)

                  gatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaa                                                                                                                          <  1:398479/141‑1 (MQ=255)

                    tAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaaga                                                                                                                        >  3:554165/1‑141 (MQ=255)

                    tAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGGTGGTAAAGGTAAAATTgaaga                                                                                                                        >  2:1508965/1‑141 (MQ=255)

                    tAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATAACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaaga                                                                                                                        >  3:181994/1‑141 (MQ=255)

                    tAAGCAATTTAATTTTGAGTCTACAATGAAGGAAATATAATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaaga                                                                                                                        >  1:880875/1‑141 (MQ=255)

                      aGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGaaa                                                                                                                      >  1:1261573/1‑141 (MQ=255)

                      aGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGaaa                                                                                                                      >  1:1143319/1‑141 (MQ=255)

                       gCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAAt                                                                                                                     <  1:817092/141‑1 (MQ=255)

                           tttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                 <  1:529789/141‑1 (MQ=255)

                           tttaattnTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAATTTGAAGAAATTCaa                                                                                                                 <  2:1244233/141‑1 (MQ=255)

                            ttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAg                                                                                                                <  1:1453093/141‑1 (MQ=255)

                             taatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGc                                                                                                               <  1:1517285/141‑1 (MQ=255)

                              aatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGAGGTCATCTTAGCAATGAAAAAAATAAAGAAATTCAAGCa                                                                                                              >  4:558582/1‑141 (MQ=255)

                              aatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCa                                                                                                              >  1:771022/1‑141 (MQ=255)

                              aatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTCGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCa                                                                                                              <  1:108942/141‑1 (MQ=255)

                                ttttGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGAATATCATCTTGGCAGCGGAAAAATTGAAGAAATTCAAGCAtt                                                                                                            >  4:401180/1‑141 (MQ=255)

                                 tttGAGTCTAAAATGGAGGAATTATCATCTTTATCAGAGACTTGTCAACTTGAAGTGTTGGGTCAAATTACTCAAATCAGAGATCGTGTAGATCGCAAATATTATGTTGGTAATGGTAAAATTGAAGAAATTCAAGCAttt                                                                                                           <  4:76851/141‑1 (MQ=255)

                                    gAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTAtt                                                                                                        <  2:167121/141‑1 (MQ=255)

                                        cTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGt                                                                                                    <  1:109931/141‑1 (MQ=255)

                                         tACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGtt                                                                                                   >  3:536562/1‑141 (MQ=255)

                                         tACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGtt                                                                                                   >  4:337645/1‑141 (MQ=255)

                                          aCAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAGAGGTCATGGTAGCAAAGGAAAAAATGAAGAAATTCAAGCATTTATTGAGTTc                                                                                                  >  2:843949/1‑141 (MQ=255)

                                          aCAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTc                                                                                                  >  4:299465/1‑141 (MQ=255)

                                            aaTGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCAt                                                                                                             <  4:140073/128‑1 (MQ=255)

                                             aTGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCaaa                                                                                               <  1:1328261/141‑1 (MQ=255)

                                               ggaggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGAGATTACCGTGGCAAAAGAAAAAATAAAGAAATTCAAGCATTTATTGAGTTCAAAGa                                                                                             >  2:1127744/1‑141 (MQ=255)

                                                 aggaATTGGCAGCTTTATCAGAGGCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGAGGGCAAATGTGATGGTGGTAGAGGTAGAATGTAGGAAATTCAAGCATTTATTGGGTTCAAAGata                                                                                           <  2:1396306/141‑1 (MQ=255)

                                                   gaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTAGTTAGAGCATATTTCGTGTATATTGTGAGTATTTGGTGGGTAAAGGTAAGATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATAtt                                                                                         >  3:601711/1‑141 (MQ=255)

                                                   gaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGAGATCACCGTAGCAAAAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATAtt                                                                                         >  4:116338/1‑141 (MQ=255)

                                                     aTTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGa                                                                                       >  3:74182/1‑141 (MQ=255)

                                                       tATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATg                                                                                     <  2:368761/141‑1 (MQ=255)

                                                        atcatcTTTATCAGAGACTTGCCAACTTGAAGTGTTGCGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAACTCAAGCATTTATTGAGTTCAAAGATATTGAttt                                                                                    <  4:585771/141‑3 (MQ=255)

                                                          catcTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAg                                                                                  <  3:627917/141‑1 (MQ=255)

                                                            tcTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCACCGTATCAATAAAAAAAATAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtc                                                                                >  4:416673/1‑141 (MQ=255)

                                                            tcTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGTGGTCGCCGTAGCATAAAAAAAAAAGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtc                                                                                >  2:1559486/1‑141 (MQ=255)

                                                              tttATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcat                                                                              <  3:453855/141‑1 (MQ=255)

                                                               ttATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                             <  4:696608/141‑1 (MQ=255)

                                                                tATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatca                                                                            <  4:446628/141‑1 (MQ=255)

                                                                 aTCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATcac                                                                           >  3:93838/1‑141 (MQ=255)

                                                                  tCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATcaca                                                                          <  1:1417596/141‑1 (MQ=255)

                                                                      agaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATTGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatg                                                                      <  1:1001597/141‑1 (MQ=255)

                                                                      agaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatg                                                                      >  1:1285226/1‑141 (MQ=255)

                                                                       gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                                     >  4:460109/1‑141 (MQ=255)

                                                                       gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                                     <  1:593136/141‑1 (MQ=255)

                                                                       gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                                     >  1:1332166/1‑141 (MQ=255)

                                                                        aCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatgat                                                                    >  4:434078/1‑141 (MQ=255)

                                                                          ttGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAAtgatga                                                                  >  2:1417548/1‑141 (MQ=255)

                                                                           ttccAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGaa                                                                 >  2:1366886/3‑141 (MQ=255)

                                                                             ccAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAAtt                                                               >  3:640401/1‑141 (MQ=255)

                                                                                   tGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                 <  3:260628/85‑1 (MQ=255)

                                                                                       gtggtgGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGca                                                     <  4:649067/141‑1 (MQ=255)

                                                                                        tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                             >  1:203707/1‑116 (MQ=255)

                                                                                        tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                             <  2:203707/116‑1 (MQ=255)

                                                                                        tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGcac                                                    <  2:746189/141‑1 (MQ=255)

                                                                                        tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGcac                                                    <  3:223160/141‑1 (MQ=255)

                                                                                          ttGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACaa                                                  <  4:353664/141‑1 (MQ=255)

                                                                                            gggTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATc                                                <  3:299465/141‑1 (MQ=255)

                                                                                            gggTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATc                                                <  3:700425/141‑1 (MQ=255)

                                                                                             ggTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATcc                                               >  3:212343/1‑141 (MQ=255)

                                                                                                  aaTTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                 >  1:43669/1‑70 (MQ=255)

                                                                                                    ttACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCAc                                        <  3:103374/141‑1 (MQ=255)

                                                                                                      aCTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTa                                      >  1:85265/1‑141 (MQ=255)

                                                                                                        tCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTaaa                                    <  2:1052304/141‑1 (MQ=255)

                                                                                                        tCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTaaa                                    >  3:160508/1‑141 (MQ=255)

                                                                                                        gcAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTaaa                                    >  1:1573299/2‑141 (MQ=255)

                                                                                                         cAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAAt                                   <  1:757402/141‑1 (MQ=255)

                                                                                                             aCAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAg                               <  1:1207466/141‑1 (MQ=255)

                                                                                                                 agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                           >  1:1127110/1‑141 (MQ=255)

                                                                                                                 agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                           >  3:512121/1‑141 (MQ=255)

                                                                                                                 agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                           >  1:221117/1‑141 (MQ=255)

                                                                                                                 agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                           >  1:978164/1‑141 (MQ=255)

                                                                                                                  gaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTa                          >  3:419742/1‑141 (MQ=255)

                                                                                                                   aTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAg                         <  1:1018456/141‑1 (MQ=255)

                                                                                                                      gtgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtg                      >  1:778988/1‑141 (MQ=255)

                                                                                                                       tgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtgt                     <  4:245421/141‑1 (MQ=255)

                                                                                                                       tgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtgt                     <  2:1143319/141‑1 (MQ=255)

                                                                                                                        gtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                    <  1:1384987/141‑1 (MQ=255)

                                                                                                                        gtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                    <  2:1444204/141‑1 (MQ=255)

                                                                                                                        gtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                    <  1:129388/141‑1 (MQ=255)

                                                                                                                           gATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTaaaa                 <  4:521563/141‑1 (MQ=255)

                                                                                                                             tCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAat               >  1:681551/1‑141 (MQ=255)

                                                                                                                               gCAAATATTATGTTGGTAAGGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAatta             <  2:427571/141‑1 (MQ=255)

                                                                                                                                    tattatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCaa                                             <  2:1301785/104‑1 (MQ=255)

                                                                                                                                      ttatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAg      <  2:457226/141‑1 (MQ=255)

                                                                                                                                      ttatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAg      <  1:672367/141‑1 (MQ=255)

                                                                                                                                       tatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGa     <  3:335527/141‑1 (MQ=255)

                                                                                                                                       tatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGa     >  1:359097/1‑141 (MQ=255)

                                                                                                                                          gTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGAACt  >  3:472249/1‑141 (MQ=255)

GGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGAACT  >  USA300TCH1516_ALE/1333657‑1333935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`