BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,857,700	A→G	5.0%	I225T (ATA→ACA)	USA300HOU_RS09235 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,857,700	0	A	G	5.0%	523.1 / 8.5	170	I225T (ATA→ACA)	USA300HOU_RS09235	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (64/95); new base G (3/6); total (68/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

CGTTTGCTAATCTTTCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAAT  >  USA300TCH1516_ALE/1857560‑1857839

cGTTTGCTAATCTTTCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGata                                                                                                                                              >  2:1445817/1‑141 (MQ=255)

  tttGCTAATCTTTCAGCTTGTGACTCTGGTTTTGATGCTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGatata                                                                                                                                            <  1:345873/141‑1 (MQ=255)

  tttGCTAATCTTTCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGatata                                                                                                                                            <  4:104768/141‑1 (MQ=255)

     gCTAATCTTTCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCa                                                                                                                                         >  2:1552997/1‑141 (MQ=255)

      cTAATCTTTCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCAc                                                                                                                                        <  3:29095/141‑1 (MQ=255)

              tCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAatta                                                                                                                                <  1:1612724/141‑1 (MQ=255)

                 gCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTAttt                                                                                                                             >  2:387189/1‑141 (MQ=255)

                  cTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTg                                                                                                                            >  1:630792/1‑141 (MQ=255)

                   ttGTGACTCTGGTTTTGATACTTCTACCCTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGc                                                                                                                           <  3:628022/141‑1 (MQ=255)

                     gtgACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTTTCTTTCTCTGGGAGTGCTTCTGGTTTGGTTTCTTGAAACTCTTGTTCCTCTTGTGTTATTTCACTTTAATTATTTGCAc                                                                                                                         >  1:157219/1‑141 (MQ=255)

                     gtgACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCAc                                                                                                                         >  1:735215/1‑141 (MQ=255)

                      tgACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACt                                                                                                                        <  1:1469929/141‑1 (MQ=255)

                       gACTCTGGTTTTGATACTTCTACCTTTGCTGCTCTTGCATCTTTGGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATGTGCACtt                                                                                                                       <  1:1023025/141‑1 (MQ=255)

                         ctctGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCGCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGc                                                                                                                     <  2:507611/141‑1 (MQ=255)

                          tctGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGct                                                                                                                    >  2:1331399/1‑141 (MQ=255)

                            tGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGcttc                                                                                                                  >  2:161982/1‑141 (MQ=255)

                            tGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGcttc                                                                                                                  <  1:173085/141‑1 (MQ=255)

                              gTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGcttctt                                                                                                                <  2:569587/141‑1 (MQ=255)

                              gTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGcttctt                                                                                                                <  2:1607860/141‑1 (MQ=255)

                              gTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGcttctt                                                                                                                <  2:148423/141‑1 (MQ=255)

                                 ttGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGcttcttc‑tt                                                                                                            <  4:435366/141‑1 (MQ=255)

                                   gATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑tttt                                                                                                          >  3:95680/1‑141 (MQ=255)

                                   gATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑tttt                                                                                                          >  1:1283383/1‑141 (MQ=255)

                                    aTACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTa                                                                                                         >  3:192368/1‑141 (MQ=255)

                                    aTACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTa                                                                                                         <  2:170495/141‑1 (MQ=255)

                                     tACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTAt                                                                                                        >  1:940183/1‑141 (MQ=255)

                                       cTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCg                                                                                                      >  2:38535/1‑141 (MQ=255)

                                        ttCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCgc                                                                                                     >  1:390468/1‑141 (MQ=255)

                                         tCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCgct                                                                                                    >  2:1622867/1‑141 (MQ=255)

                                              cTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCttg                                                                                               <  4:577117/141‑1 (MQ=255)

                                                 tgctgcACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTTATCGCTGCttgtt                                                                                             >  1:131304/1‑141 (MQ=255)

                                                 tgctgcACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCttgttg                                                                                            >  3:584601/1‑141 (MQ=255)

                                                   ctgcACTTGCATCTTTAGTTGATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTtgtg                                                                                          >  3:450208/1‑141 (MQ=255)

                                                   ctgcACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATTTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTtgtg                                                                                          >  2:799582/1‑141 (MQ=255)

                                                    tgcACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGc                                                                                         <  4:705709/141‑1 (MQ=255)

                                                    tgcACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGc                                                                                         <  1:1286342/141‑1 (MQ=255)

                                                       aCTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCttt                                                                                      >  1:1355546/1‑141 (MQ=255)

                                                       aCTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCttt                                                                                      >  2:850419/1‑141 (MQ=255)

                                                            cATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATg                                                                                 >  3:358525/1‑141 (MQ=255)

                                                            cATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATg                                                                                 <  3:412564/141‑1 (MQ=255)

                                                            cATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATg                                                                                 <  3:412775/141‑1 (MQ=255)

                                                              tCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGcc                                                                               <  1:850419/141‑1 (MQ=255)

                                                               cTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCa                                                                              <  1:725844/141‑1 (MQ=255)

                                                               cTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCa                                                                              >  1:1175515/1‑141 (MQ=255)

                                                                     ttAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtt                                                                        <  2:1434250/141‑1 (MQ=255)

                                                                       aaTGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCCTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                                      <  2:878453/141‑1 (MQ=255)

                                                                         tgtgTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTgg                                                                    <  2:1228901/141‑1 (MQ=255)

                                                                         tgtgTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTgg                                                                    <  1:564380/141‑1 (MQ=255)

                                                                          gtgtCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTgat                                                                   >  2:635214/1‑139 (MQ=255)

                                                                          gtgtCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGt                                                                   <  3:677951/141‑1 (MQ=255)

                                                                          gtgtCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGt                                                                   <  1:1057063/141‑1 (MQ=255)

                                                                            gtCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGttc                                                                 <  2:1144308/141‑1 (MQ=255)

                                                                             tCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGttct                                                                >  3:263198/1‑141 (MQ=255)

                                                                             tCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGttct                                                                <  1:518261/141‑1 (MQ=255)

                                                                                cTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCa                                                             <  4:450208/141‑1 (MQ=255)

                                                                                   tCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATATATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTga                                                                    >  2:248725/1‑130 (MQ=9)

                                                                                   tCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCAttt                                                          <  2:316934/141‑1 (MQ=255)

                                                                                     tttCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGa                                                        >  1:525624/1‑141 (MQ=255)

                                                                                      ttCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGAt                                                       >  1:299175/1‑141 (MQ=255)

                                                                                            gCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCttt                                                                       >  1:587975/1‑119 (MQ=255)

                                                                                              ttGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGc                                               >  4:439190/1‑141 (MQ=255)

                                                                                                  gCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCAc                                                                             <  2:958605/107‑1 (MQ=40)

                                                                                                  gCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCAc                                                                             >  1:958605/1‑107 (MQ=255)

                                                                                                  gCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACtt                                           >  2:858612/1‑141 (MQ=255)

                                                                                                  gCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTTAt                                                            >  4:622176/1‑124 (MQ=16)

                                                                                                   cttcttTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACttt                                          <  1:1453817/141‑1 (MQ=255)

                                                                                                    ttcttTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACtttt                                         <  1:705780/141‑1 (MQ=255)

                                                                                                      cctttttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTAt                                       >  1:694632/3‑141 (MQ=255)

                                                                                                         ttttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTg                                    <  4:73112/141‑1 (MQ=255)

                                                                                                         ttttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTg                                    <  1:1646145/141‑1 (MQ=255)

                                                                                                           ttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGtt                                  <  3:377807/141‑1 (MQ=255)

                                                                                                             aGCTTCTTGCACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTc                                <  1:1453111/141‑1 (MQ=255)

                                                                                                             aGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTg                                                <  3:432089/125‑1 (MQ=255)

                                                                                                             aGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTg                                                >  4:432089/1‑125 (MQ=255)

                                                                                                             aGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTc                                <  3:206739/141‑1 (MQ=255)

                                                                                                              gCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGc                                                                                         <  2:1491849/83‑1 (MQ=25)

                                                                                                                   ttGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGTTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTtg                          <  2:454289/141‑1 (MQ=255)

                                                                                                                     gAACCTCTTGTGCCTCTTGTGATATATCACTTAATTTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTtgtg                        <  2:104183/141‑1 (MQ=255)

                                                                                                                     gAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTtgtg                        <  4:364320/141‑1 (MQ=255)

                                                                                                                      aaCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGc                       <  4:586103/141‑1 (MQ=255)

                                                                                                                      aaCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGc                       <  1:1105773/141‑1 (MQ=255)

                                                                                                                      aaCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCGTTCAATGCGGCTGCTTTTGATTCTTGATTTGAGACAGCTGCGCTTTTCGTTGTTTTTTCTTGTGc                       >  2:669464/1‑141 (MQ=14)

                                                                                                                      aaCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑ATTTATCGCGGCTTTTGGTTCACTCAATGCCACGGCTATTGTTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGc                       >  3:420297/1‑141 (MQ=17)

                                                                                                                       aCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGct                      <  1:866296/141‑1 (MQ=255)

                                                                                                                       aCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGct                      <  1:1353973/141‑1 (MQ=255)

                                                                                                                        cctcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGctt                     <  1:1642515/141‑1 (MQ=255)

                                                                                                                        cctcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGctt                     <  3:483668/141‑1 (MQ=255)

                                                                                                                         ctcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTTATTTGATACAGCTACACTTTTATCCGTTTCTGCTTGTGcttc                    >  4:475708/1‑141 (MQ=255)

                                                                                                                         ctcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCCGCACTTTTATCCGCTTCTGCTTGTGcttc                    >  4:635328/1‑141 (MQ=255)

                                                                                                                          tcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTATTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGcttct                   <  3:424728/141‑1 (MQ=255)

                                                                                                                            ttgtgcctcttgtgTTATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttt                 <  2:1316325/141‑1 (MQ=255)

                                                                                                                            ttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTtgtg                                                                                          <  4:148713/68‑1 (MQ=40)

                                                                                                                            ttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttt                 <  1:387189/141‑1 (MQ=255)

                                                                                                                            ttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttt                 <  3:49901/141‑1 (MQ=255)

                                                                                                                            ttgtgcctcgtgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTTTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttt                 <  3:565280/141‑1 (MQ=255)

                                                                                                                              gtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCGCTTCTTTTTTTTTATCTTTTGATTCGGCAGCGCTTCTATCAGTTTCTGCTTGTGcttttgtgt               >  2:357012/1‑135 (MQ=14)

                                                                                                                              gtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttttt               <  1:858612/141‑1 (MQ=255)

                                                                                                                              gtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttttt               <  3:620873/141‑1 (MQ=255)

                                                                                                                                         gATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCCGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGa    <  3:473465/141‑1 (MQ=255)

                                                                                                                                            atatCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGAttc                                                                 >  2:747942/1‑77 (MQ=0)

CGTTTGCTAATCTTTCAGCTTGTGACTCTGGTTTTGATACTTCTACCTTTGCTGCACTTGCATCTTTAGTTAATGTGTCACTGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAAT  >  USA300TCH1516_ALE/1857560‑1857839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`