Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,211,670 C→A 5.1% intergenic (+663/+150) USA300HOU_RS11275 → / ← yidC hypothetical protein/Membrane protein insertase YidC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,211,6700CA 5.1% 682.9 / 14.7 200intergenic (+663/+150)USA300HOU_RS11275/yidChypothetical protein/Membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base C (115/74);  new base A (6/4);  total (121/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 211 total aligned reads displayed.

CATATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGAC  >  USA300TCH1516_ALE/2211531‑2211810
                                                                                                                                           |                                                                                                                                             
cATATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTcaca                                                                                                                                              <  4:194213/141‑1 (MQ=255)
cATATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTcaca                                                                                                                                              <  2:620825/141‑1 (MQ=255)
   ataCTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAttt                                                                                                                                           >  2:1352350/1‑141 (MQ=255)
                  tgaATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTActg                                                                                                                           <  2:258274/141‑1 (MQ=255)
                     aTCATTGTGTCAAGATGACATTGCGATGAAGAATGACAGCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTActgctg                                                                                                                        >  1:876727/1‑141 (MQ=255)
                      tCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGt                                                                                                                       >  2:612057/1‑141 (MQ=255)
                        aTTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGttt                                                                                                                     >  3:594722/1‑141 (MQ=255)
                          tgtgTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCt                                                                                                                   >  1:682545/1‑141 (MQ=255)
                           gtgtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCATTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTCTCTa                                                                                                                  >  2:372018/1‑141 (MQ=255)
                            tgtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAt                                                                                                                 >  1:1577611/1‑141 (MQ=255)
                             gtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTTTTTTTTTTAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAtt                                                                                                                >  2:1061608/1‑141 (MQ=255)
                             gtCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAtt                                                                                                                >  1:626224/1‑141 (MQ=255)
                              tCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTAc                                                                                                                             >  1:1246640/1‑127 (MQ=255)
                              tCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTAc                                                                                                                             <  2:1246640/127‑1 (MQ=255)
                              tCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTAttt                                                                                                               >  3:503052/1‑141 (MQ=255)
                                  gATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATca                                                                                                           >  1:969413/1‑141 (MQ=255)
                                  gATGACATTGCGATGAAGAATGACAACTCTGTTATTAAACACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGTTGTTTATATTTTTca                                                                                                           >  2:151300/1‑141 (MQ=255)
                                    tGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCAGATTTGAAATCATCTTACTGCTGTTTCTATTTATcaca                                                                                                         <  1:1197070/141‑1 (MQ=255)
                                     gACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACat                                                                                                        >  2:1488555/1‑141 (MQ=255)
                                      aCATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACata                                                                                                       <  4:631060/141‑1 (MQ=255)
                                      aCATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACata                                                                                                       <  2:1386327/141‑1 (MQ=255)
                                        aTTGCGATGAACAACGACAACTCTGTTATTAAACACTATTTACATACTGAAAACTCGTTTATATTATTTCAACTAAATACAACAGTAGTATGACTTTAACATTTGAATTTATCATACTGCTGTTTCTAATTATcacgtgtt                                                                                                     <  4:677715/141‑6 (MQ=255)
                                          tGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGt                                                                                                   >  2:414893/1‑141 (MQ=255)
                                          tGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGt                                                                                                   <  1:1321800/141‑1 (MQ=255)
                                              aTGAATAATGACAACTCTGTTATTAACCACGTTTTACATACTTAAAACCCCTTAATATTATTGCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATaa                                                                                               <  1:152223/141‑1 (MQ=255)
                                                gaagaaTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAAtg                                                                                             <  2:126756/141‑1 (MQ=255)
                                                   gaaTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGa                                                                                          >  2:862462/1‑141 (MQ=255)
                                                     aTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGaca                                                                                        <  1:819811/141‑1 (MQ=255)
                                                     aTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGaca                                                                                        >  2:133035/1‑141 (MQ=255)
                                                     aTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGaca                                                                                        <  3:202218/141‑1 (MQ=255)
                                                      tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                       <  3:622103/141‑1 (MQ=255)
                                                      tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                       >  2:1639426/1‑141 (MQ=255)
                                                      tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                       >  1:398340/1‑141 (MQ=255)
                                                       gACAACTCTGTTATTAACCACTTTTTACATACTGAAATCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACt                                                                                      <  4:625526/141‑1 (MQ=255)
                                                       gACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGGTGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATTGTGTGACACt                                                                                      >  1:193582/1‑141 (MQ=255)
                                                        aCAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTGTATTTATCACATATTGTATAATGTGACACTa                                                                                     >  1:780885/1‑141 (MQ=255)
                                                           aCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGt                                                                                  >  4:562338/1‑141 (MQ=255)
                                                            ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTActgc                                                                                                                          >  2:180057/1‑100 (MQ=255)
                                                            ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGtt                                                                                 >  3:349946/1‑141 (MQ=255)
                                                            ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGtt                                                                                 >  1:393530/1‑141 (MQ=255)
                                                             tctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGttt                                                                                >  3:702563/1‑141 (MQ=255)
                                                               tGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCg                                                                              >  1:1048386/1‑141 (MQ=255)
                                                                gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCCCATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc                                                                             <  1:68963/141‑1 (MQ=255)
                                                                gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc                                                                             <  4:138762/141‑1 (MQ=255)
                                                                gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc                                                                             >  1:1068462/1‑141 (MQ=255)
                                                                 ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCt                                                                            <  1:414893/141‑1 (MQ=255)
                                                                   attaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCGGGTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTAt                                                                          >  2:1423475/1‑141 (MQ=255)
                                                                     taACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTg                                                                        <  3:11723/141‑1 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGa                                                                       >  1:444242/1‑141 (MQ=255)
                                                                      aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGa                                                                       >  3:45564/1‑141 (MQ=255)
                                                                       aCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaa                                                                      >  1:1121442/1‑141 (MQ=255)
                                                                       aCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaa                                                                      <  4:610172/141‑1 (MQ=255)
                                                                        ccACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCttcaatag                                                                     <  1:431886/141‑8 (MQ=255)
                                                                         cACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGc                                                                    <  3:642260/141‑1 (MQ=255)
                                                                            tttttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaatcaaa                                                                 >  4:594323/1‑136 (MQ=255)
                                                                              tttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGaaaa                                                               <  4:11077/141‑1 (MQ=255)
                                                                                 aCATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAaata                                                            >  2:806567/1‑141 (MQ=255)
                                                                                  cATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGATAGTGAAGCGAAAaataa                                                           >  3:572456/1‑141 (MQ=255)
                                                                                     aCTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAAtgt                                                        >  2:735476/1‑141 (MQ=255)
                                                                                       tGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgc                                                      >  3:284193/1‑141 (MQ=255)
                                                                                        gAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcg                                                     <  2:1568936/141‑1 (MQ=255)
                                                                                           aaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATTATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGccc                                                  <  1:524780/141‑1 (MQ=255)
                                                                                             cTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCta                                                <  1:1440786/141‑1 (MQ=255)
                                                                                              tCGTTAATATTATTTCAAATCAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTACCACATATTGTATAATGTGACACTACGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCtat                                               <  4:227225/141‑1 (MQ=255)
                                                                                              tCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcgcaaaaa                                               <  2:444242/141‑6 (MQ=255)
                                                                                                gTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGACGCGAAAAATAATGTGCGCCCTATaa                                             >  1:491993/1‑141 (MQ=255)
                                                                                                gTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATaa                                             >  4:437037/1‑141 (MQ=255)
                                                                                                gTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATaa                                             >  2:114410/1‑141 (MQ=255)
                                                                                                gTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATaa                                             >  3:470683/1‑141 (MQ=255)
                                                                                                  tAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTGTCTATTTATAACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTTCGCCCTATAAAg                                           >  4:398569/1‑141 (MQ=255)
                                                                                                       ttattTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTaaa                                      >  2:537372/1‑141 (MQ=255)
                                                                                                         attTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAAt                                    >  3:322372/1‑141 (MQ=255)
                                                                                                            tCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTAt                                 >  3:279995/1‑141 (MQ=255)
                                                                                                              aaaTAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCt                               >  2:750106/1‑141 (MQ=255)
                                                                                                              aaaTAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCt                               >  1:205519/1‑141 (MQ=255)
                                                                                                                 tagaaaCAGCAGTAAGATGATTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCa                            >  4:254101/4‑141 (MQ=21)
                                                                                                                 tAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTcc                            >  2:1184740/1‑140 (MQ=255)
                                                                                                                 tAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCa                            >  1:1476666/1‑141 (MQ=255)
                                                                                                                 tAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCa                            <  2:1458520/141‑1 (MQ=255)
                                                                                                                   gaaaCAGCAGTAAGATGATTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTaaa                                      <  2:684356/128‑1 (MQ=21)
                                                                                                                   aaaaCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAAc                          >  3:654622/1‑141 (MQ=255)
                                                                                                                   aaaaCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAAc                          <  2:222299/141‑1 (MQ=255)
                                                                                                                    aaaCAGCAGTAGGATGACTTTAACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAATTTAACATTATCTTCAACt                         >  2:1135363/1‑141 (MQ=255)
                                                                                                                     aaCAGCAGTAGGATGACTCTCACATTTGAAAACACCTTACTGCTGTTTCAATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATAGAAGCGAAAAAAAAAGTGCGCAATATAAAGTTAAAATTACCATCaaatc                        >  2:772595/1‑138 (MQ=255)
                                                                                                                      aCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACttt                       <  3:706450/141‑1 (MQ=255)
                                                                                                                      aCAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCACTATAAAGTTAAAATTATCTTCAACttt                       >  4:502814/1‑141 (MQ=255)
                                                                                                                       cagcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACtttt                      >  3:683659/1‑141 (MQ=255)
                                                                                                                       cagcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACtttt                      >  2:961296/1‑141 (MQ=255)
                                                                                                                       cagcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACtttt                      <  2:199220/141‑1 (MQ=255)
                                                                                                                         gcagTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAg                    >  2:105149/1‑141 (MQ=255)
                                                                                                                           agTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAggg                  <  2:1452528/141‑1 (MQ=255)
                                                                                                                           agTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAggg                  <  1:363637/141‑1 (MQ=255)
                                                                                                                                gATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATCTTTCGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcac             <  1:29465/141‑1 (MQ=255)
                                                                                                                                gATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcac             <  2:976376/141‑1 (MQ=255)
                                                                                                                                 gatgatttCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATGATCTTCAACTTTTAGGGTGcac             >  1:340743/6‑141 (MQ=37)
                                                                                                                                  tGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACat           <  4:683659/141‑1 (MQ=255)
                                                                                                                                    aCTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACatta         <  2:334591/141‑1 (MQ=255)
                                                                                                                                        tCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGCGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTg     <  2:1003738/141‑1 (MQ=255)
                                                                                                                                          acaTTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGt                                                                                                   >  3:6140/1‑45 (MQ=255)
                                                                                                                                          acaTTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGa   >  2:499526/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CATATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAATCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGAC  >  USA300TCH1516_ALE/2211531‑2211810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: