Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 9,710 T→C 5.2% intergenic (+15/+72) gyrA → / ← nnrD DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE9,7100TC 5.2% 805.3 / 22.3 261intergenic (+15/+72)gyrA/nnrDDNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (129/117);  new base C (4/10);  total (133/128)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 265 total aligned reads displayed.

GGAAATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACA  >  USA300TCH1516_ALE/9570‑9849
                                                                                                                                                   |                                                                                                                                           
ggAAATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGGATGGAAGTAAGACAAGAGTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACtt                                                                                                                                             >  1:242640/1‑141 (MQ=255)
  aaaTGCAATTCATACTGAAGTGATTGATTCAGAGGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTcc                                                                                                                                           >  1:862576/1‑141 (MQ=255)
   aaTGCAATTCATACTGAAGTGATTGATTCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTcccc                                                                                                                                          >  2:652638/1‑141 (MQ=255)
       cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                      >  3:148936/1‑141 (MQ=255)
       cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                      <  4:407086/141‑1 (MQ=255)
          ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCtacatat                                                                                                                                   <  1:1475247/141‑6 (MQ=255)
          ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCtacatat                                                                                                                                   <  3:377609/141‑6 (MQ=255)
           tCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTa                                                                                                                                  >  1:1018938/1‑141 (MQ=255)
            cATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAg                                                                                                                                 <  1:209961/141‑1 (MQ=255)
            cATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAg                                                                                                                                 >  3:91367/1‑141 (MQ=255)
              tACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGAATTAATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAggg                                                                                                                               <  1:989799/141‑1 (MQ=255)
               aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAgggg                                                                                                                              <  2:537640/141‑1 (MQ=255)
                cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                             >  3:299658/1‑141 (MQ=255)
                cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                             >  4:668831/1‑141 (MQ=255)
                 tGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAg                                                                                                                            >  1:1435619/1‑141 (MQ=255)
                   aaGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTc                                                                                                                          <  1:430334/141‑1 (MQ=255)
                    aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCt                                                                                                                         <  2:1515124/141‑1 (MQ=255)
                    aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCt                                                                                                                         <  3:18345/141‑1 (MQ=255)
                      tgattgatTCAGAAGAAAATGATGAAGATGGACGGATTGAAGTAAGACAAGATTCCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTa                                                                                                                       >  4:463343/1‑141 (MQ=255)
                        attgattCAGAAGAGAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATGTGTAGGGGAGTCTTAtt                                                                                                                     <  2:1196891/141‑1 (MQ=255)
                        attgattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAtt                                                                                                                     <  1:1135943/141‑1 (MQ=255)
                          tgattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAtttt                                                                                                                   >  1:913244/1‑141 (MQ=255)
                          tgattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAtttt                                                                                                                   <  1:1570864/141‑1 (MQ=39)
                          tgattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAtttt                                                                                                                   >  2:1570864/1‑141 (MQ=39)
                            attCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTa                                                                                                                 <  1:294959/141‑1 (MQ=255)
                            attCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTa                                                                                                                 <  1:1042287/141‑1 (MQ=255)
                              tCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATg                                                                                                               >  3:491916/1‑141 (MQ=255)
                                agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCt                                                                                                             <  2:1101084/141‑1 (MQ=255)
                                agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAttttttatta                                                                                                             <  1:5265/141‑6 (MQ=38)
                                 gaagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTa                                                                                                            >  4:676817/1‑141 (MQ=255)
                                  aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAg                                                                                                           <  2:632763/141‑1 (MQ=255)
                                  aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAg                                                                                                           <  2:71410/141‑1 (MQ=255)
                                         tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                    >  1:638265/1‑141 (MQ=255)
                                          gatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGt                                                                                                      >  1:1222787/1‑138 (MQ=255)
                                          gatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGAGGAGCCGTATTTTTATGCTAGAAAGTaaa                                                                                                   >  2:16555/1‑140 (MQ=255)
                                            tggaGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGc                                                                                                 <  2:193143/138‑1 (MQ=255)
                                              aaGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCtt                                                                                               <  3:280041/141‑1 (MQ=255)
                                                gATGGACGTAGTGAAGTAAGACAAGATTTCATGGACCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGGCTGATTCTTATGCTAGAAAGTANTGCTTTa                                                                                             >  2:1240817/1‑141 (MQ=255)
                                                  tGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACt                                                                                           <  4:100620/141‑1 (MQ=255)
                                                  tGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTACATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACt                                                                                           >  4:272994/1‑141 (MQ=255)
                                                       gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATAtt                                                                                      <  1:1356280/141‑1 (MQ=255)
                                                         aTTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCa                                                                                    >  3:551939/1‑141 (MQ=255)
                                                            gAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATg                                                                                 >  1:599042/1‑141 (MQ=255)
                                                            gAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTGCCCTATAGTTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATg                                                                                 <  3:336060/141‑1 (MQ=255)
                                                            gAAGTAAGACAAGATTTAATGGGTCGTGGTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTGTACtata                                                                                        >  4:533063/1‑141 (MQ=255)
                                                              aGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGAt                                                                               <  2:446112/141‑1 (MQ=255)
                                                                 aaGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAGAAAAAATAAGTCTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAg                                                                            >  1:163667/1‑141 (MQ=255)
                                                                   gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                          >  1:1264033/1‑141 (MQ=255)
                                                                   gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                          >  3:220294/1‑141 (MQ=255)
                                                                     cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGCAGAATAATAAAAACTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGACAGTAATGCTTTACTATATTCAATGATTAGTAAt                                                                        >  2:33850/1‑141 (MQ=255)
                                                                      aaGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATg                                                                       <  1:111706/141‑1 (MQ=255)
                                                                       aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                      <  1:1107768/141‑1 (MQ=255)
                                                                        gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                     >  1:116411/1‑141 (MQ=255)
                                                                        gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                     <  2:1126795/141‑1 (MQ=255)
                                                                            tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAGAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                 >  4:298567/1‑141 (MQ=255)
                                                                             cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                                >  4:694984/1‑141 (MQ=255)
                                                                             cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                                >  4:51669/1‑141 (MQ=255)
                                                                               tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAt                                                                                                                      >  4:545936/1‑85 (MQ=25)
                                                                                ggATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTc                                                             <  2:1070758/141‑1 (MQ=255)
                                                                                    cGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAGTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAt                                                         >  2:851755/1‑141 (MQ=255)
                                                                                     gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                        <  2:1617661/141‑1 (MQ=255)
                                                                                      tgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg                                                       >  2:1431376/1‑141 (MQ=255)
                                                                                        ttGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAACAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGtt                                                     <  2:1580234/141‑1 (MQ=255)
                                                                                        ttGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGCTTAGTAAGGACTAACTATCTAATTGtt                                                     >  1:723010/1‑141 (MQ=255)
                                                                                          gaagaaGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTc                                                   <  4:334878/141‑1 (MQ=255)
                                                                                              aagaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTg                                               <  2:242646/141‑1 (MQ=255)
                                                                                               agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                              >  2:747057/1‑141 (MQ=255)
                                                                                                gaTATACAACAATTATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                             <  4:593113/141‑1 (MQ=255)
                                                                                                gaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                             >  1:1298220/1‑141 (MQ=255)
                                                                                                 atatACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGt                                            >  2:408242/1‑141 (MQ=255)
                                                                                                   ataCAACAATCATC‑‑‑‑‑‑AGTTGAAGAATAATTAAAAATAAGACTTCCCTATATGTAGGGGAGACTTATTTGTATGCTAGAAAGTAATGCTTTACGATATTCACTTATTAGTATTGACTAACTTCCAATTTGTTACATAGCGTaa                                          >  4:43598/1‑141 (MQ=255)
                                                                                                   ataCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACta                                                                                          >  4:359478/1‑93 (MQ=255)
                                                                                                    taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATAtt                                                                                      >  2:1350775/1‑96 (MQ=255)
                                                                                                    taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTACTGACTAACTTTCTAATTGTTTCATTGCGTacg                                         >  2:553456/1‑139 (MQ=255)
                                                                                                    taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAg                                         >  2:797326/1‑141 (MQ=255)
                                                                                                    taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAg                                         <  1:1331129/141‑1 (MQ=255)
                                                                                                      caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                       <  1:828923/141‑1 (MQ=255)
                                                                                                       aacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTa                                      >  4:201362/1‑141 (MQ=255)
                                                                                                            tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                 <  2:812082/141‑1 (MQ=255)
                                                                                                             catt‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGTCTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTACGGTATTTCAt                                <  4:502718/141‑1 (MQ=255)
                                                                                                               tc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTg                              >  2:1538118/1‑141 (MQ=255)
                                                                                                                c‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                             >  2:347293/1‑141 (MQ=255)
                                                                                                                c‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                             >  1:1193400/1‑141 (MQ=255)
                                                                                                                       aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTc                                                                                                                          >  1:948904/1‑47 (MQ=255)
                                                                                                                       aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTc                                                                                                                          <  2:948904/47‑1 (MQ=255)
                                                                                                                       aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                            <  1:780550/141‑1 (MQ=255)
                                                                                                                         aTGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATaa                          >  2:1425398/1‑141 (MQ=255)
                                                                                                                          tGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATCTCCTTGATAAg                         <  2:760064/141‑1 (MQ=255)
                                                                                                                            aagaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTc                       <  1:341219/141‑1 (MQ=255)
                                                                                                                             agaaTAATAAAAAATAAAACTTCCCTATATGTAGGGGAGTCTTATTTTTGTGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                      <  2:1461137/141‑1 (MQ=255)
                                                                                                                              gaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCtt                     >  1:490415/1‑141 (MQ=255)
                                                                                                                                    taaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggt               >  3:521312/1‑141 (MQ=255)
                                                                                                                                    taaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggt               >  2:986377/1‑141 (MQ=255)
                                                                                                                                        aaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGc            <  2:1264080/141‑1 (MQ=255)
                                                                                                                                           aTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTatat                                                                                       <  3:62279/63‑1 (MQ=255)
                                                                                                                                              aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTg                              <  1:1598240/117‑1 (MQ=255)
                                                                                                                                              aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca      >  2:496400/1‑141 (MQ=255)
                                                                                                                                                 cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac   <  3:43598/141‑1 (MQ=255)
                                                                                                                                                 cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac   <  2:1320696/141‑1 (MQ=255)
                                                                                                                                                 cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac   <  1:1538118/141‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GGAAATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACA  >  USA300TCH1516_ALE/9570‑9849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: