Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,588 G→A 5.2% S1230S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5880GA 5.2% 935.4 / 2.5 403S1230S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (178/179);  new base A (25/14);  total (205/198)
Fisher's exact test for biased strand distribution p-value = 9.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 408 total aligned reads displayed.

CAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCG  >  USA300TCH1516_ALE/618455‑618721
                                                                                                                                     |                                                                                                                                     
  gattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                                                     <  1:2366505/134‑1 (MQ=255)
   attcagatagagatgaagaTTCAGATAGTGATGCAGACTGAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGAc                                                                                                                                    <  2:1505677/134‑1 (MQ=14)
   attcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGTGACTCAGATTTATATAGCGAATCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGAc                                                                                                                                    <  1:7668/134‑1 (MQ=17)
         acagcgactcagaCTCAGATATTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                                                                                              <  1:2035737/134‑1 (MQ=255)
           agcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTTAGACATCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                                                                            <  2:1734698/134‑1 (MQ=255)
            gcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGTGATTCa                                                                                                                           >  1:1169298/1‑134 (MQ=255)
                 tcatgctcagaTAGGGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                                                                                      <  2:2037952/129‑1 (MQ=255)
                 tcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGAAAGTGACTCAGATTCAGATAGCCACTCAGACTCGGCCAGCGATTCAGCCTc                                                                                                                      >  1:1204333/1‑134 (MQ=14)
                   agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAACGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                                                                                    >  1:975527/1‑134 (MQ=255)
                        cagaTAGTGATTGAGACTCAGATAGCGGCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGc                                                                                                               <  1:1095490/134‑1 (MQ=255)
                          gaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                                             <  1:525457/134‑1 (MQ=255)
                             aGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACACAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                                          <  2:1129062/134‑1 (MQ=255)
                                gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGCCTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                                       >  1:1373810/1‑134 (MQ=255)
                                   tCATACTCAGATAGTGTCTCGGAAGCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattc                                                                                                    <  2:1147873/134‑1 (MQ=14)
                                    cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattca                                                                                                   <  2:2710329/134‑1 (MQ=255)
                                      gattCAGATAGCGACTTAGATTAAGACAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                                 <  2:1774816/131‑1 (MQ=255)
                                      gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                                 >  2:2772987/1‑134 (MQ=255)
                                      gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACGCAGATTCAGATAGCGACTCAGATTCGGACAGAGATTCAGACTCATATAGCGACtcagattcaga                                                                                                 <  1:558975/134‑1 (MQ=255)
                                       aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                                                >  1:862141/1‑134 (MQ=255)
                                       aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACACAGATACGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                                                >  1:1685558/1‑134 (MQ=255)
                                        cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATa                                                                                               >  1:2306332/1‑134 (MQ=255)
                                          aaaacaGCGAATCAGATTTAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc                                                                                             <  1:2527623/129‑1 (MQ=255)
                                           aGATAGCGACTCAGATTCCGACAGCGATTCAGACTCAGACATCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                                            <  1:445611/134‑1 (MQ=255)
                                            gATAGCGACTTAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                                           <  2:2687478/134‑1 (MQ=255)
                                               agggACTGAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc                                                                                        <  2:1442018/131‑1 (MQ=255)
                                               aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAAACAGCCATCTATTCATATTTAGCGACTCAGATTCAGATAGCGAtac                                                                                        >  2:599762/1‑132 (MQ=14)
                                               aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGAATCAGATAGCGAATCAAACTCAGACAGCGAATCAGACACAAATAGCGACTCAGATTCAGAGAGAGACTc                                                                                        >  2:2376962/1‑134 (MQ=11)
                                                gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCg                                                                                       <  1:2452450/134‑1 (MQ=255)
                                                  gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                     <  1:1323270/134‑1 (MQ=255)
                                                   aCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                    <  1:1764984/134‑1 (MQ=255)
                                                         aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                              >  1:2052678/1‑134 (MQ=255)
                                                         aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                              <  1:1430401/134‑1 (MQ=255)
                                                          ttCATACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATa                                                                             <  1:371315/134‑1 (MQ=255)
                                                              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                         >  1:1003564/1‑134 (MQ=255)
                                                              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                         >  1:2559412/1‑134 (MQ=255)
                                                              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                         >  1:1702488/1‑134 (MQ=255)
                                                              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                         >  1:1158070/1‑134 (MQ=255)
                                                              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                         <  1:1854286/134‑1 (MQ=255)
                                                               acagcgactcagactcagacagcgactcagactcagATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                        <  1:2772987/134‑1 (MQ=255)
                                                                  gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtca                                                                     >  2:1254412/1‑134 (MQ=255)
                                                                   cgactcagactcagacagcgactcagactcagaTAGTGACTTTGATTCAGATAGCGACTAAGATTCGTACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                                    <  1:2716469/134‑1 (MQ=255)
                                                                   cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATACAGACTCAGAAACAGACtcaa                                                                    >  2:1401066/1‑133 (MQ=14)
                                                                     actcagactcagatagcgactcagactcagaTAGTGACTCAGATTCAGATAGTGATTTAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                  <  1:1655400/134‑1 (MQ=255)
                                                                     actcagactcagacagcgactcagactcggtTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                  <  1:1480239/134‑1 (MQ=255)
                                                                        cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGTCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattca                                                               <  1:943500/134‑1 (MQ=255)
                                                                        cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGATAGCGATTCGGACTCAGATAGCGACacagattca                                                               >  1:2496982/1‑134 (MQ=255)
                                                                        cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAATCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATATCGACtcagattca                                                               >  2:256576/1‑134 (MQ=255)
                                                                         agactcaggcagcgactcagattcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                              <  2:1940439/134‑1 (MQ=255)
                                                                               aggcagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                                        <  2:1527702/131‑1 (MQ=255)
                                                                               agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCATATTCGGAAAGAGAGTTAAACTCAGATAGCGACTCAGATCAAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAAAGTg                                                        >  2:1035491/1‑134 (MQ=17)
                                                                                gacagcgactgagacacagaTAGTGTGTCATTTTGAGATAGCGTCTCACATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                       <  2:1565991/134‑1 (MQ=17)
                                                                                gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                       <  1:822094/134‑1 (MQ=255)
                                                                                gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCCGATAGCGACTCAGATTCAGATAGTGa                                                       >  1:241006/1‑134 (MQ=255)
                                                                                 acagcgattcatactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                      <  1:1052681/134‑1 (MQ=255)
                                                                                  tagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                     <  2:1659694/133‑1 (MQ=255)
                                                                                  cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                     <  1:912865/134‑1 (MQ=255)
                                                                                  cagcgactcagactcagaTAGTGACTCAGATGCAAATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAAAGAAACAGACACAGATAGAGACTCAGAGTCACATAGTGAtt                                                     >  2:626741/1‑134 (MQ=14)
                                                                                   cgctactcagactcagaGAGAGACGCAGATTCAGCTAGCCATGCACATGCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTTATTc                                                    <  2:1293939/130‑1 (MQ=14)
                                                                                        ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGCACTCAGATAGCGACTCAGATTCAGATAGTGATACAGAca                                               >  2:1801837/1‑133 (MQ=255)
                                                                                         tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTc                                              >  1:308335/1‑134 (MQ=255)
                                                                                         tcagactcagaTAGTGACTCAGAGTCAGATAGCGAATCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAATGATGCAGACTc                                              >  1:420020/1‑134 (MQ=255)
                                                                                          cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                             <  1:2840129/134‑1 (MQ=255)
                                                                                          cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                             <  1:1728924/134‑1 (MQ=255)
                                                                                              tgcagaGAGGGATTCAGATTTCGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                                         <  2:2454831/132‑1 (MQ=14)
                                                                                               tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                        <  1:637858/134‑1 (MQ=255)
                                                                                               tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                        <  2:1188434/134‑1 (MQ=255)
                                                                                                cagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCACATAGCGACACGCACTCAGATAGCGACCCAGATTCAGATAGCGATTCAGACTCAGATaca                                       >  2:216329/1‑132 (MQ=14)
                                                                                                  gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGa                                     >  1:2343371/1‑134 (MQ=255)
                                                                                                  gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATACCGATTCGGACTCAGATAGCGACTCAGATTCAGAAAGTGATTCAGACTCAGATAGCGa                                     >  2:476371/1‑134 (MQ=255)
                                                                                                     agggcttcagattcagatAGCGACTGAGATTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                  <  2:333307/128‑1 (MQ=17)
                                                                                                     aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCTGACTCAGATAGCTACTCAGATTAAGATAGTGATTCAGACTCAGATAACAACTc                                  >  1:1943674/1‑134 (MQ=14)
                                                                                                     aGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCACATTAAGAGAGTTACTCAGACCCAAATAGCGAATc                                  >  2:315120/1‑134 (MQ=14)
                                                                                                      gTGATTCAGATTAAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                 <  1:2748884/134‑1 (MQ=255)
                                                                                                       tGACTCTGATTCAAAGAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAg                                <  1:1791136/134‑1 (MQ=255)
                                                                                                        gACTCCGATTCAGATAGCGACTCAGATTCTGACAGCGTTTTAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa                               <  2:853515/134‑1 (MQ=255)
                                                                                                          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGAACTCAGATAGAGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAtt                             >  1:2204934/1‑134 (MQ=255)
                                                                                                               attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAGTCAGATTCAGAc                        >  1:1603932/1‑134 (MQ=255)
                                                                                                               attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                        >  2:2316715/1‑134 (MQ=255)
                                                                                                               attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                        <  1:2003735/134‑1 (MQ=255)
                                                                                                                ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTGAGACTCAGATAGCGACTCAGATTCAGACa                       >  2:820515/1‑134 (MQ=255)
                                                                                                                 tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                      >  2:1849172/1‑134 (MQ=255)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCCAATACTGATTCAGACTCAGATAGCGAATCAGACTCAGACAACGa                   >  2:531039/1‑134 (MQ=14)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                   >  1:548456/1‑134 (MQ=255)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                   >  1:34918/1‑134 (MQ=255)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                   >  1:2176084/1‑134 (MQ=255)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                   >  2:2227329/1‑134 (MQ=255)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAATCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                   >  1:153707/1‑134 (MQ=255)
                                                                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTACGACTCAGCTAGCGACTCAGATACAGATAGTGATTCAGACTCCGCTACCTAGTCAGAGTCAGACAGCGa                   >  2:732195/1‑134 (MQ=14)
                                                                                                                     aaaGCGATGCAGATTCAGACAGTGTCGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc                  <  2:2623732/132‑1 (MQ=11)
                                                                                                                         cGACTCAGATTCGGACAGCTATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg              <  1:138890/134‑1 (MQ=255)
                                                                                                                         cGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg              <  1:2643329/134‑1 (MQ=255)
                                                                                                                             tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTc          >  1:2122672/1‑134 (MQ=255)
                                                                                                                             tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAATCAGATCCAGATACCGACACAGACCCCGATCCCGACTCAGACTCCGAAAACAACTCAGaccc          >  2:673066/1‑131 (MQ=11)
                                                                                                                             tCAGATTCGGACAGCGATTCAGACGCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAGTCAGATAGCGACTCAGATTCAGACAGCGAGTCaaaatg          >  2:2510329/1‑129 (MQ=14)
                                                                                                                                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGa       >  1:485206/1‑134 (MQ=255)
                                                                                                                                 aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAt      >  1:1248606/1‑134 (MQ=255)
                                                                                                                                 aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAAAGCGACTCAGAATCGGAt      >  2:789917/1‑134 (MQ=255)
                                                                                                                                   tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg    >  1:2149968/1‑134 (MQ=255)
                                                                                                                                    cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc   >  1:713251/1‑134 (MQ=255)
                                                                                                                                     ggACAGCGATTCAGACTAAGATAGCGAATCAGATTCAGATAGCGATTCGGACTGAGATAGCGAATCAGATACAGATAGTGATTAAGAATAAGAAAAAGAATCAGAATCAGAAAGCGAATAAGATTAGGATAGCg  >  1:2265355/1‑134 (MQ=14)
                                                                                                                                     |                                                                                                                                     
CAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCG  >  USA300TCH1516_ALE/618455‑618721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: