Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,636 G→A 6.6% S1246S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6360GA 6.6% 843.1 / 3.4 381S1246S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (166/152);  new base A (31/12);  total (207/174)
Fisher's exact test for biased strand distribution p-value = 1.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 383 total aligned reads displayed.

GCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCA  >  USA300TCH1516_ALE/618503‑618768
                                                                                                                                     |                                                                                                                                    
gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCg                                                                                                                                      <  1:2452450/134‑1 (MQ=255)
   aCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                                   <  1:1764984/134‑1 (MQ=255)
     tctgATTCAGACAGCGACTCAGACTCAGACAGCGGCTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                 <  2:1515714/131‑1 (MQ=255)
     tCAGATTCAGACAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                 <  1:539772/134‑1 (MQ=255)
        gATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGa                                                                                                                              >  1:434306/1‑134 (MQ=255)
         atgcagaaagcgactcagactcagacagcgactcagactCAGATAGTGATTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                             <  1:2394149/131‑1 (MQ=255)
         aTTCAGGCCGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                             <  2:1807991/134‑1 (MQ=255)
              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGGCTGTCTATTGCTGATAGCGACTAAGATTCAGATAGCGATTCGGAATCAGATAGCGa                                                                                                                        >  1:1348891/1‑134 (MQ=17)
              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                        >  1:1003564/1‑134 (MQ=255)
              gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                        <  1:1854286/134‑1 (MQ=255)
              gacagcgactcagactcagacagcgactcagactcaGATAGAGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCCGATAGCGACACAGATTCACACAGCGACACCGACTCAGCCAGCGa                                                                                                                        >  2:1267218/1‑134 (MQ=11)
               atagcgattcagattcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAAATAGCGAc                                                                                                                       >  2:1896145/3‑134 (MQ=14)
               acagcgactcagactcagacagcgactcagactcagATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                       <  1:2772987/134‑1 (MQ=255)
                 agcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                     <  1:1743347/134‑1 (MQ=255)
                 agcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                     <  1:1975288/134‑1 (MQ=255)
                 agcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                     <  1:2223419/134‑1 (MQ=255)
                   cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCCGAAAGCGATTCAGACTCAGATAGCGAATCAGACTCAGATAGAGAATCAAACTTAGAAAGCGaagcga                                                                                                                   >  2:116434/1‑129 (MQ=14)
                     actcagactcagatagcgactcagactcagaTAGTGACTCAGATTCAGATAGTGATTTAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                                                                 <  1:1655400/134‑1 (MQ=255)
                     actcagactcagacagcgactcagactcggtTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                                                                 <  1:1480239/134‑1 (MQ=255)
                       tcagactaagacggcgactcagactcagaTAGTGACTTATATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                                               <  2:643555/134‑1 (MQ=255)
                           actcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                                                                                           >  1:1392717/1‑134 (MQ=255)
                               agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTg                                                                                                       >  1:2742867/1‑134 (MQ=255)
                                gacagcgactgagacacagaTAGTGTGTCATTTTGAGATAGCGTCTCACATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                                      <  2:1565991/134‑1 (MQ=17)
                                 acagcgattcatactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                                                                     <  1:1052681/134‑1 (MQ=255)
                                  cagcgactcagactcagaTAGTGAGTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                                    <  2:2573010/134‑1 (MQ=255)
                                   agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTc                                                                                                   <  2:2353193/134‑1 (MQ=255)
                                   agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTc                                                                                                   >  1:950500/1‑134 (MQ=255)
                                   agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGCGATTCGGAATAAGATAGCGACACAGAATCAGATAGCGAtta                                                                                                   >  2:969998/1‑133 (MQ=14)
                                   acggactcagactcagaTAGTGAATCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTc                                                                                                   <  2:2520186/131‑1 (MQ=255)
                                    gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                                                                                  <  1:2292023/134‑1 (MQ=255)
                                    gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAACGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                                                                                  >  1:877032/1‑134 (MQ=255)
                                     cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCTGACTCAAATAGCGACTCAGATTCAGACAGTGATTCAg                                                                                                 >  2:1536925/1‑134 (MQ=255)
                                     cgactcagactcagaTAGCGACTCACATTTAGATAGCGACTCAAAGACATACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAg                                                                                                 <  2:318949/134‑1 (MQ=14)
                                      gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGCTTCAGATAGTGATTCAGa                                                                                                >  1:761101/1‑134 (MQ=255)
                                        ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACt                                                                                              >  1:1431885/1‑134 (MQ=255)
                                         tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                             >  1:1540856/1‑134 (MQ=255)
                                         tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTc                                                                                             >  1:308335/1‑134 (MQ=255)
                                          cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                            <  1:1728924/134‑1 (MQ=255)
                                           agactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGCGTCAGATAGCGAATCAGACTTAGATAGCGACTCAGACTCGGATAGCGACTCAGAATCaa                                                                                           >  2:2736876/1‑133 (MQ=14)
                                            gactcggaTAGTGCTTCAGATGAAGATAGCGTCTCAGATTCGGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                          <  2:544614/134‑1 (MQ=14)
                                              gttagaTAGTGGCTGAGCGTCATATAGCGATTCAGATTCGAACAAGAATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                                                                                        <  2:2479774/131‑1 (MQ=14)
                                               tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                                                                       <  1:637858/134‑1 (MQ=255)
                                                cagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCACATAGCGACACGCACTCAGATAGCGACCCAGATTCAGATAGCGATTCAGACTCAGATaca                                                                                      >  2:216329/1‑132 (MQ=14)
                                                  gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGa                                                                                    >  1:829230/1‑134 (MQ=255)
                                                   aTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                                                                   <  1:2504693/134‑1 (MQ=255)
                                                      gTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                                                                <  1:2187710/134‑1 (MQ=255)
                                                       tGACTCTGATTCAAAGAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAg                                                                               <  1:1791136/134‑1 (MQ=255)
                                                        gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGAACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa                                                                              >  1:2117244/1‑134 (MQ=255)
                                                          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCATATAGCGACTCAGATTCAGATAGTGATTTAAAATCAGATAGCGACTAAGAtt                                                                            >  1:1158356/1‑134 (MQ=14)
                                                            cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAAATTCACATAGTTATTCAGACTCCAATACCAAATCAGATTCa                                                                          >  2:79287/1‑134 (MQ=11)
                                                             agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                         >  2:1452206/1‑134 (MQ=255)
                                                             agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATATCGACTCAGATTCAg                                                                         >  1:168900/1‑134 (MQ=255)
                                                               attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAGTCAGATTCAGAc                                                                       >  1:1603932/1‑134 (MQ=255)
                                                               attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                       >  1:391180/1‑134 (MQ=255)
                                                                 tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                     >  2:1849172/1‑134 (MQ=255)
                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCATATAGCGACTCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACaacaa                                                                  >  1:920573/1‑130 (MQ=255)
                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                  >  1:34918/1‑134 (MQ=255)
                                                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTACGACTCAGCTAGCGACTCAGATACAGATAGTGATTCAGACTCCGCTACCTAGTCAGAGTCAGACAGCGa                                                                  >  2:732195/1‑134 (MQ=14)
                                                                     attgCGACTGAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc                                                                 <  2:2690383/131‑1 (MQ=255)
                                                                      tAGCGACTGAGATTCAGACATCGATTCAGACTCAGATAGCGACTAAGATTCAGATAGAGATTCGGATTCAGATAGAGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                <  1:1185652/134‑1 (MQ=17)
                                                                         cGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                                                             <  1:2643329/134‑1 (MQ=255)
                                                                             tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGATAGTGCTTCAGCATCATAGAGGGACTCAGATTCAGACAGCGACTCAGATTc                                                         <  1:555273/134‑1 (MQ=17)
                                                                             tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAATCAGATCCAGATACCGACACAGACCCCGATCCCGACTCAGACTCCGAAAACAACTCAGaccc                                                         >  2:673066/1‑131 (MQ=11)
                                                                                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGa                                                      >  1:485206/1‑134 (MQ=255)
                                                                                 aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATACGGACTCACCTAGCGACTCAGATTGAAATAGTAATGAACCATCAGATAGCGAATCAGCTTCAGAAAAAGAATCAGAtaaaaaa                                                     >  2:393652/1‑128 (MQ=9)
                                                                                   tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                                   >  1:2149968/1‑134 (MQ=255)
                                                                                    cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                  >  1:713251/1‑134 (MQ=255)
                                                                                    cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                  <  1:1227677/134‑1 (MQ=255)
                                                                                      gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                             <  1:204804/57‑1 (MQ=40)
                                                                                       tcattaaTTCTGACGCAGACAGCGACTCATACTCAGATAGTGATTCGGACTCAGATAGCGACTCAGATTCTGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                                               <  2:1757142/128‑1 (MQ=17)
                                                                                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATACAGATCGTGATTCAGACTCAGATAGCGACTAAGATTCAGACCGCGACTCATATTCGGAAACCGACTCa                                            >  2:843619/1‑134 (MQ=17)
                                                                                           cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAg                                           >  1:2822813/1‑134 (MQ=255)
                                                                                            gATTCAGACTCAGAGTCCGACTTAGATTCAGATTGCTATTCCGATTCAGATAGCGACTCAGATTCAGATAGGGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGa                                          <  2:2847021/134‑1 (MQ=14)
                                                                                               acAGACTCAGATAGCGACTTAGATTCAGATAGCGATTCTGTCTCAGATAGTGACTTTGATTCAGATAGTGATGTGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTc                                       <  1:201173/133‑1 (MQ=14)
                                                                                                cAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                      <  1:2066496/134‑1 (MQ=255)
                                                                                                cAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                      <  1:2551224/134‑1 (MQ=255)
                                                                                                  gACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGATTTAGATAGCGACTCAGATTCAGAAAGCGACTCAGATTCGGAAAGAGGATAAGATTCata                                    >  2:585100/1‑132 (MQ=14)
                                                                                                  gACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGa                                    >  1:2515845/1‑134 (MQ=255)
                                                                                                       aGATCGCGGCTCAGATTCAGATAGCGATTAGGACTCTGATAGTGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGCCTCAGATTCGGATAGCGACTCAGATTCAGACAGCg                               <  2:1112163/134‑1 (MQ=14)
                                                                                                        gATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGTGACTCAGATTCAGATAGTGATTCAGACTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa                              <  1:999224/134‑1 (MQ=255)
                                                                                                          tAGCGACTCAGATTCAGATAGCAATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACACAGATTCAGATAGCGACTCAGACTCAGACAGCGACt                            >  2:1197900/1‑134 (MQ=11)
                                                                                                           aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCGGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                           <  1:1104811/134‑1 (MQ=255)
                                                                                                            gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGCTGGCGACTCTGAGCCTGACATCGACGCCCACTCCTACAGCGACTCAGATTCCGCCAGCgcgtca                          >  2:1022449/1‑129 (MQ=9)
                                                                                                             cGACTCAGATTCAGATAGCGATTCGGACTCAGATATCGACTCAGATTCAGATACTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                         >  1:1710291/1‑134 (MQ=255)
                                                                                                              gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCAACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                        >  1:2006066/1‑134 (MQ=255)
                                                                                                                 tcagattcagatAGCGATTCGGACTCAGATAGCGATTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc                     <  1:2598123/134‑1 (MQ=255)
                                                                                                                 tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc                     <  1:1914031/134‑1 (MQ=255)
                                                                                                                 tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAcac                     >  2:2291194/1‑132 (MQ=255)
                                                                                                                 tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATACGGATAGCGAATCAGATTCAGACAACGAACCAGAccc                     >  2:1983508/1‑132 (MQ=17)
                                                                                                                 tcagattcagatAGCGATTCCGACTCAGATAGCGACTCAGATGCAGATAGTGATTCAGAATCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGAATAGAGACACAGATTCAGACAGCGACTCAGACTc                     >  2:2412259/1‑134 (MQ=17)
                                                                                                                    gattctgatGGCGATTTGGATTCAGATTGCGACTTAGATTCAGATAGTGATTCAGACTCAGATAGCGACTGAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                  <  2:491889/134‑1 (MQ=18)
                                                                                                                    gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGCTAGCGACTCAGACTCAGACAGCGACTCAGACTCGGa                  >  2:1700742/1‑134 (MQ=255)
                                                                                                                    gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                  <  1:1316664/134‑1 (MQ=255)
                                                                                                                    gattcagatAGAGATACAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                  <  2:1318035/134‑1 (MQ=255)
                                                                                                                          gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGa            >  2:1645905/1‑134 (MQ=255)
                                                                                                                           atAGCGATTTGTGATCAGATAGCGACTCAGATTCAGATAGTGATTTAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt           <  1:818234/134‑1 (MQ=18)
                                                                                                                              gCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCa        >  1:2342156/1‑134 (MQ=255)
                                                                                                                               cGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCCGATAGTGATCCAg       >  2:1356779/1‑134 (MQ=255)
                                                                                                                               cGATTCGAACTCAGTTTGGTACTCACATTCAGATTGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg       <  1:519232/134‑1 (MQ=18)
                                                                                                                                    cGGACTCAGATAGCGACTCATATTCAGATTGTGCTTGACCTACAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCa  <  1:2705481/134‑1 (MQ=18)
                                                                                                                                     |                                                                                                                                    
GCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCA  >  USA300TCH1516_ALE/618503‑618768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: