Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,359 T→C 5.1% D974D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,3590TC 5.1% 915.6 / 11.2 389D974D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (185/171);  new base C (20/4);  total (211/178)
Fisher's exact test for biased strand distribution p-value = 2.70e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 421 total aligned reads displayed.

CACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAT  >  USA300TCH1516_ALE/622226‑622491
                                                                                                                                     |                                                                                                                                    
 acTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTTAGATTCAGACAGCGACtcagatt                                                                                                                                     >  1:1083219/1‑134 (MQ=255)
   ttGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattca                                                                                                                                   <  2:2669195/134‑1 (MQ=255)
          cGGATACTTCGAAGAAGATACAGCAGAAAGCGATTCATACTTAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGACAGCg                                                                                                                            <  2:2313416/134‑1 (MQ=21)
           ggATACTTCGAAGAAGATACATCAGACAGCAATTCACACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCATATTCAGACAGCGACTCAGATTCAGATAGCGa                                                                                                                           <  2:72347/134‑1 (MQ=255)
           gcattattCTAAGAAGATACATCAGACATCGATTCATATTCAGATAGTGACTAAGACAGCGACTCAGACGCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGa                                                                                                                           <  1:1207268/128‑1 (MQ=18)
            gATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAc                                                                                                                          <  1:217374/134‑1 (MQ=255)
            gATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAc                                                                                                                          <  1:1023237/134‑1 (MQ=255)
            gATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAc                                                                                                                          >  1:474600/1‑134 (MQ=255)
              tACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTc                                                                                                                        >  1:2201573/1‑134 (MQ=255)
                cTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCCGACTCAGACAGCGACTCAGACTCAGACACTGATTCAGATTCAGACAGCGACTCAGATCCAGATAACGACTCAg                                                                                                                      >  2:1251055/1‑134 (MQ=255)
                cTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAg                                                                                                                      >  1:1780488/1‑134 (MQ=255)
                cTTCGAAGAAGATAAATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAg                                                                                                                      <  1:2682410/134‑1 (MQ=255)
                  tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAAAGCGACTCAGAGTCAGATATCGACTCAGAt                                                                                                                    >  1:449909/1‑134 (MQ=255)
                  tCGAAGAAGATAAATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAt                                                                                                                    <  1:2395737/134‑1 (MQ=255)
                   cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAtt                                                                                                                   >  1:92153/1‑134 (MQ=255)
                   cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACACAGACTCAGATAGCGACTCAGact                                                                                                                   >  2:67403/1‑132 (MQ=255)
                    gaagaaGATACATCAGACATCGATTCATACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACGCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTc                                                                                                                  <  1:1902098/134‑1 (MQ=255)
                    gaagaaGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTc                                                                                                                  >  1:2594267/1‑134 (MQ=255)
                     aagaagATACATCAGACAGCGATTCATACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATGCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCg                                                                                                                 <  1:1740174/134‑1 (MQ=255)
                        aagaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGAc                                                                                                              <  2:287872/134‑1 (MQ=255)
                          gaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                            >  1:2307270/1‑134 (MQ=255)
                          gaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                            >  1:762701/1‑134 (MQ=255)
                             aCATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGTCTCAGACAGTGACTCAGACGCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                         <  1:1130995/134‑1 (MQ=255)
                             aCATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                         <  1:99626/134‑1 (MQ=255)
                              cATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                                                                        <  1:1939525/134‑1 (MQ=255)
                                tCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                                                      <  1:1698879/134‑1 (MQ=255)
                                tCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGAATCAGACAGTGATTCAGATACACCCACAGATCAAGATTCAGATAGCGACTCAGACTCCGAGAACGATTc                                                                                                      >  2:604818/1‑134 (MQ=18)
                                   gACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                   >  1:274890/1‑134 (MQ=255)
                                   gACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                   >  1:1289130/1‑134 (MQ=255)
                                   gACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGAGACACAGATTCCGATAGCTACTCAGATTCGCATCGCGAtgccga                                                                                                   >  2:162587/1‑129 (MQ=18)
                                    aCAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGAATCAGACTCAGACACTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAc                                                                                                  <  1:2345367/134‑1 (MQ=255)
                                       gtgATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                                                               <  2:968092/132‑1 (MQ=255)
                                        cGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                                                              >  2:2370914/1‑134 (MQ=255)
                                        cGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                                                              >  1:1507149/1‑134 (MQ=255)
                                         gATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                                                             <  1:2793332/134‑1 (MQ=255)
                                          aTTTAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGAt                                                                                            <  1:377495/134‑1 (MQ=255)
                                          aTTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACCCCGACTCAGATTCAGACAGCCACTCAGACTCAGATAGCGACTCAGAATCAGACAGCGATTCAGACTAAgct                                                                                            >  2:1584202/1‑132 (MQ=17)
                                             cAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGc                                                                                         >  1:1920183/1‑134 (MQ=255)
                                               gACTCTGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                       <  2:772323/134‑1 (MQ=255)
                                               gACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATCGCGa                                                                                       >  1:835962/1‑134 (MQ=255)
                                                aCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGAGATTCAGACACAGACAGCGACTCAGATCCAGACAGCGACTCAGACACAGACACCCACCCAAACACAGATACCGAc                                                                                      >  2:1690952/1‑134 (MQ=14)
                                                aCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACACAGACTCAGACAGTCGTATGCGTTAATCGATCTGCTCGGTTTCAAAAACCCACACAGATGCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                      <  1:678169/134‑1 (MQ=12)
                                                  tctgATAGTGTCTCAGACTGCGCCTCAGACTCAGACAGCGACTGAGCGTCAGACAGTGATTCAGATTCAGACTGCGACTCAGATTAAGACAGCGACTCAGCTTCAGACAGCGATTCAGACTCAGATAGCGACtc                                                                                    <  2:1570989/131‑1 (MQ=14)
                                                  tCAGATAGTGACTCAGAGAGGGACTCAGACTCAGACAGTGACTTAGACTCAGACAGTGATTCAGATGCAGACAGCGACTCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                    <  1:343797/134‑1 (MQ=17)
                                                  tCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCGGACAGCGAATCAGACTCAGACAGCGACtc                                                                                    >  2:2095681/1‑134 (MQ=14)
                                                   cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca                                                                                   >  2:1440902/1‑134 (MQ=255)
                                                    aGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag                                                                                  <  1:1569668/134‑1 (MQ=255)
                                                    aGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag                                                                                  <  1:2825683/134‑1 (MQ=255)
                                                     gATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTTAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                 <  1:1238953/134‑1 (MQ=255)
                                                     gATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                 >  2:2597480/1‑134 (MQ=255)
                                                      aTAGTGACTCAGAAAGAGACTCTGACTCAGACAGCGACTCAGACTCAGACAGAGAGAGAGATTCAGACAGCGACTCAGACTCAGATAGCGAATGAGATTCAGACAGCGATTGAGACTCAGAAAGCGACgcagac                                                                                >  2:2513190/1‑133 (MQ=14)
                                                        aGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACtcagattc                                                                              >  2:1090400/1‑134 (MQ=255)
                                                        aGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTAAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGCAtcagatta                                                                              >  1:195960/1‑133 (MQ=255)
                                                          tGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcag                                                                            <  2:474600/134‑1 (MQ=255)
                                                           aactcagacagcgactcagactcatacagcgactcagACTCAGACAATGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                           <  2:1299107/133‑1 (MQ=255)
                                                            actcagacagcgactcagactcagacagcgactcagACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                          >  1:2040655/1‑134 (MQ=255)
                                                              tcagacagcgactcagactcagacagcgactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                        >  1:2286564/1‑134 (MQ=255)
                                                               cagacagcgactcagactcagacagcgactcagactCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc                                                                       <  2:1027805/134‑1 (MQ=255)
                                                                  acagcgactcagactcagacagcgactcagactcagACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                    <  1:1523852/134‑1 (MQ=255)
                                                                  acagcgactcagactcagacagcgactcagactcagACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                    >  1:1174270/1‑134 (MQ=255)
                                                                  acagcgactcagactcagacagcgactcagactcagACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGAGAGTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                    >  2:1936444/1‑134 (MQ=255)
                                                                  acagcgactcagactcagacagcgactcagactcagACAGTGATTCAGATTCAGACAGCGACTCAGATGCAGATAGCGACTCAGATTCAGACCGCGACGCAGAGTCTGAGAGCGAGCCAGATTCAGAAAGAGAt                                                                    >  2:857366/1‑134 (MQ=11)
                                                                  acagcgactcagactcagacagcgactcagactcagACAGTGATTCAGAATCAGACACCGACTCAGATTCAGCTAGCGACTCAGATTCGGACAGAGAGTCAGACACAGAGAGCGACTCAGATTCACATAGCGac                                                                    >  2:174236/1‑133 (MQ=14)
                                                                    agcgactcagactcagacagcgactcagactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAGTCAGATTCGGAAAGCGATTCAGACTCAGATAGCAAAGCCGATTCAGATAGCGAtac                                                                  >  2:2275676/1‑132 (MQ=14)
                                                                      cgactcagactcagacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGAc                                                                                                              >  2:520249/1‑88 (MQ=255)
                                                                      cgactcagactcagacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTAAGATAGCGATtcag                                                                >  1:814785/1‑134 (MQ=255)
                                                                      cgactcagactcagacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                                                                                              <  2:789841/88‑1 (MQ=25)
                                                                       gactcagactcagacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                                                                                                               <  2:687883/86‑1 (MQ=25)
                                                                           acgactcagatatcgactaagacttagacagTGATTGAGAGTCAGACAGCGAGTCAGATGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactca                                                           <  2:1883929/132‑1 (MQ=14)
                                                                                  agatagcgactcagactcagacagTGAGTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCg                                                    <  2:1139858/134‑1 (MQ=255)
                                                                                  agacagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCg                                                    <  2:2195322/134‑1 (MQ=255)
                                                                                     cagcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACCCAGATTCAGATAGCGATTCAGACTCAGACAGCGACt                                                 >  1:2530271/1‑134 (MQ=255)
                                                                                      agtgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATCGCGATTCGGACTCAGACAGCAACtc                                                >  2:2453027/4‑134 (MQ=255)
                                                                                      agcgactcatactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtc                                                <  1:423097/134‑1 (MQ=255)
                                                                                      agcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAGTCAGACAGAGAccc                                                >  2:1340560/1‑132 (MQ=14)
                                                                                      agcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACACCGACAGCGACtc                                                >  1:1877882/1‑134 (MQ=255)
                                                                                      agcgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAAATAGCGACTCAGATTCAGATAGCGATGCAGACTCAGACAGCGACtc                                                >  1:565863/1‑134 (MQ=255)
                                                                                      agcgacacagagtcagaaagTGAGTCAGATGCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAGTCAGACTCAGAGAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtc                                                <  2:2738010/134‑1 (MQ=14)
                                                                                        cgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcag                                              <  1:2573502/134‑1 (MQ=255)
                                                                                        cgactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAATCAGACAGAGATTCAGACACAGATAGCGACTCAGATTCAGATAGAGACTCAGACACAGACAGCGACtcag                                              >  2:637510/1‑134 (MQ=14)
                                                                                         gactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcaga                                             >  1:1369169/1‑134 (MQ=255)
                                                                                             acgacttatacagTGCTTCAGATTCAGATAGAGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca                                         <  1:2869565/132‑1 (MQ=14)
                                                                                               gactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCCGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattcaga                                       >  1:2475085/1‑134 (MQ=255)
                                                                                                     gacagTGATTCAGATTCAGACAGCGACTCATATTCATATAGCGACTCAGATTCGGACAGCGATTCAGACACAGATAGCCACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                 <  1:732248/134‑1 (MQ=17)
                                                                                                      atagTGATTCATATTCAGACAGCGATTCAGATTCAGATAGTTACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                <  1:2017098/132‑1 (MQ=17)
                                                                                                      acagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGac                                >  2:2413029/1‑133 (MQ=255)
                                                                                                              tcagattcagaCAGCTACTCAGATTCAGATATCGACTCAGATTCGGATAGCGATTCAGACTCAGATAGCGAATCAGAGTAAGATAGAGATTCAGACTCAGACAGCAACTCAGATTCAGATAGCGATTCGGACTc                        <  1:625317/134‑1 (MQ=14)
                                                                                                              tcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                        <  1:1639824/134‑1 (MQ=255)
                                                                                                                    tcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTAAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg                  >  1:1050738/1‑134 (MQ=255)
                                                                                                                       gaCAGCGATTCAGGTTCAGATAGCGACTCAGATTCGGACATCGATTCAGACTCAGATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGCAACTGATTCTGACTCAGACAGCGa               <  2:355060/134‑1 (MQ=14)
                                                                                                                       gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGa               >  1:2456665/1‑134 (MQ=255)
                                                                                                                         cAGCGACTGAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAtt             <  2:1170222/134‑1 (MQ=255)
                                                                                                                          aGCGACTCAGATTCAGATAGCGTCTCATATTCTTATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc            <  1:896198/134‑1 (MQ=14)
                                                                                                                          aGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc            <  2:405024/134‑1 (MQ=255)
                                                                                                                             gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGa         <  1:2131076/134‑1 (MQ=255)
                                                                                                                             gACTCAGATGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGa         <  1:1832923/134‑1 (MQ=255)
                                                                                                                               cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTAAGACAGCGATTAAGACt       >  1:1273917/1‑134 (MQ=255)
                                                                                                                               cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAAAGAACAACGTGTAGAGAGCGAATCAGAGTCAGACAGCGACTCAGACt       >  2:2030445/1‑134 (MQ=11)
                                                                                                                                tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc      <  1:1398992/134‑1 (MQ=255)
                                                                                                                                   gattcagatAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa   <  2:1419072/134‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAT  >  USA300TCH1516_ALE/622226‑622491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑