| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 622,464 | G→A | 9.7% | S1009S (TCG→TCA) | sdrE → | Serine‑aspartate repeat‑containing protein E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 622,464 | 0 | G | A | 9.7% | 793.3 / 14.1 | 393 | S1009S (TCG→TCA) | sdrE | Serine‑aspartate repeat‑containing protein E |
| Reads supporting (aligned to +/- strand): ref base G (151/175); new base A (36/18); total (188/205) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
Only 100 of 398 total aligned reads displayed.
GTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAG > USA300TCH1516_ALE/622331‑622597 | cctcagattcagaCAGCTACTCAGATTCAGATAGCGATTCAGATTCGGAAAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc < 1:2376113/132‑1 (MQ=255) tcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTAAGAATCAACTAGCGATGCGAACTc > 2:1516999/1‑134 (MQ=17) taagattcagaCAGCTACTCAGATTCAGTTATCTACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc < 1:539550/132‑1 (MQ=17) attcacaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAc < 1:1196558/134‑1 (MQ=255) tcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTAAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg > 1:1050738/1‑134 (MQ=255) gaCAGAGAATCAGATACAAATAGCTATACAGATGCGGACAGCGATTCAGACTCAACTGGCGACTGAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGa < 2:333517/134‑1 (MQ=14) tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGTTTCAGATAGCGATTAGGACTCAGAGAGCGATTCAGACTc > 2:788726/1‑134 (MQ=255) tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc < 1:1398992/134‑1 (MQ=255) gattcagatAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa < 2:1419072/134‑1 (MQ=255) cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGc < 2:2843053/134‑1 (MQ=255) gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga > 1:2302217/1‑134 (MQ=255) gacaGCGATGCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTAAGATACCTATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga < 2:2488202/131‑1 (MQ=1) tgCGACTCAGACTAGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc < 2:2594267/133‑1 (MQ=255) aggtaCTAAGACTCAGACAGCGATTCATACTTAGACAGCTACTGAGACTCACATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATCGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc < 2:1061054/130‑1 (MQ=11) aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGCTAGCgactc > 2:2164979/1‑134 (MQ=255) cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAGAGCgactcag > 1:2790186/1‑134 (MQ=255) tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc < 1:1964576/134‑1 (MQ=255) tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCgactcagaccc > 2:814199/1‑132 (MQ=255) tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAATCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCgactcggactc > 2:834418/1‑134 (MQ=255) gaGATTAGGGCGGGGATGCGGACGCAGATAGCGACTCAGATTCGGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATGCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactca < 2:296219/133‑1 (MQ=11) gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga < 1:2665513/134‑1 (MQ=255) tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACTGTGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg < 1:978915/134‑1 (MQ=255) cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAAAGATTCAGACACAGATAGCGAATCAGACTCAGACAGc > 1:1113629/1‑134 (MQ=255) cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACAAAGAAAACGAGTCAGACTCAGATAGCGACTCACACTCAGACAGc > 1:2704035/1‑134 (MQ=14) ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCg > 1:2029561/1‑134 (MQ=255) gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGAACTCAGAAACCGATTCAGACTCAAATACAGACCCAGACTCAGACAGCgc > 2:2446990/1‑133 (MQ=14) aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc < 1:1321703/134‑1 (MQ=255) aGCGATTTAGACGCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc < 2:1179557/134‑1 (MQ=255) aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc < 1:2743862/134‑1 (MQ=255) aGCGAAGCAGACGCAGATAGCGACTCAGGTTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc < 2:2493649/134‑1 (MQ=14) gCGATTCAGACTCAGATAGCGACTCAGATTTAGATAGCTATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc‑‑‑a < 1:1306759/134‑1 (MQ=255) cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGGCTCAGATAGCGACTCAGACTCAGACAGCGACtc‑‑‑ag < 1:1597921/134‑1 (MQ=255) gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTTAGACTCAGACAGTGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc‑‑‑aga < 1:700484/134‑1 (MQ=255) gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGatacagc > 2:85044/1‑128 (MQ=255) cAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACCCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc‑‑‑agattca > 1:412171/1‑134 (MQ=255) aGATAGCGACTCAGATTCAGATAGCGATTCAGCCTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑ACATACAGATAGCg > 2:2540902/1‑134 (MQ=255) aTAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCATATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGAt < 1:34153/134‑1 (MQ=255) aGCGACTCAGATTCAGATAGCGATTCAGACTCATACAGCGACTCAGATTCTGATATCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTc < 1:165308/134‑1 (MQ=255) aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTc < 1:1253315/134‑1 (MQ=255) gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCC‑‑‑AGACTCAGATCGcaactca > 2:2342849/1‑128 (MQ=14) tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTc > 1:634218/1‑134 (MQ=255) cgagaatcagaaAGCGATTCAGGGTCAGACAGCGACTCAGATTGAGATAGCGATTCAGACACAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTc < 2:1246519/132‑1 (MQ=11) cagattcagagAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCa < 2:1868515/134‑1 (MQ=255) gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCTATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGa < 1:1622170/134‑1 (MQ=255) attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATACCGAGTCGGCCTCAGaa > 2:651506/1‑133 (MQ=255) gcagagAGCGATTCAGACTCACACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAg < 2:1684630/133‑1 (MQ=255) aaatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCg < 2:35452/132‑1 (MQ=255) gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGa > 1:1850605/1‑134 (MQ=255) gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAATC‑‑‑AGATTCAGATAGCGATTCAGACTCAGATAGCGa > 1:1280178/1‑134 (MQ=255) gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGACTCAGATAGCGATTCGGACTCAGATAGCGa > 2:1806952/1‑134 (MQ=255) aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTc < 1:286793/134‑1 (MQ=255) aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTc < 1:2596999/134‑1 (MQ=255) aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAt < 1:1786422/134‑1 (MQ=255) aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGAt < 1:2261023/134‑1 (MQ=255) tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTc > 2:573075/1‑134 (MQ=255) cagcctcagacAGCGACTCAGATTCAGATAGCTATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCa < 1:929884/134‑1 (MQ=255) cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCTACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTAAGATTCa > 1:249102/1‑134 (MQ=255) gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa < 1:143296/134‑1 (MQ=255) gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa < 1:2409397/134‑1 (MQ=255) gactcagacAGCGACACAGAGTCAGATAGCGATGCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa < 2:1931360/134‑1 (MQ=255) actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGAATCAGATAGCGATTCAGACTCAGATAGCGACtcagactcagac > 2:174765/1‑134 (MQ=255) actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGAATCAGATAGAGATTCGGAGTCAGAAAACGACTCAAATTCAGAc > 2:398843/1‑134 (MQ=14) actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAAACTAAAAAAGCAACTC‑‑‑AGATACAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc > 1:724590/1‑134 (MQ=14) tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGCCTCAGCCTCAGACAGCGACTC‑‑‑AGATTCAGATAACGATAAAAACTCAAAAAAAGACTCAGATTCAGACAg > 1:474341/1‑134 (MQ=14) gcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg < 1:2332064/133‑1 (MQ=255) cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGACTCAGACTCAAAGAGCGACTCAGAATCaaaaaga > 2:161527/1‑128 (MQ=11) cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCAACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAAAGc > 1:67797/1‑134 (MQ=255) gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa < 1:1687280/134‑1 (MQ=255) acAGCGACTCAGATCCAGATAGCGATTCGGACTCAGAAAGCGATTCAGACTCAGATAGCGACTCAGAATAAGACAGCGACTC‑‑‑AGATCCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt < 1:788726/134‑1 (MQ=17) agggACTGAGAGTCAGATAGCGATGCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc < 2:2004109/131‑1 (MQ=17) taCTCAGATTCAGATACCGATTCGGACTCAGACAGCGATTTAGATTTTGTTAGTGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa < 1:251237/133‑1 (MQ=17) gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGCTTCAgc > 1:2464169/1‑133 (MQ=255) acgcagatgcagagTGCGATGGAGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc < 2:1167938/131‑1 (MQ=14) aCTCAGATTCAGATAGCGATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc < 1:2148521/134‑1 (MQ=255) cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt < 1:426585/134‑1 (MQ=255) cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt < 1:1930066/134‑1 (MQ=255) tctgagtcagagAGCGACTCGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCATACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc < 2:2698698/128‑1 (MQ=14) tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTAGGACTAAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc > 1:1993219/1‑134 (MQ=255) tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATACAAAAAACAAATCAGACTCAGAAAACGACCAGGAATCAAACACCGATTCAGAccc > 2:705998/1‑132 (MQ=14) acagatgcagatAACGATTGAGACTCATACAGCGATTCAGACTCAGAGAGCGACTCAGACTCAGACAGCGATTC‑‑‑AGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc < 2:1366494/133‑1 (MQ=11) cagattaagatAGCGATTAGGACTCAGACAGAGATTCAGACTAAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa < 1:1159482/134‑1 (MQ=255) agattcagatAGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGACTTATACTCAGATAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg < 1:1001207/134‑1 (MQ=255) gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGACTCATATAGTTACTCGGACTCAGATAGCGACTCAGATCCCGACAGCCATTCCGACCCaca > 2:1022851/1‑132 (MQ=14) attcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCATACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt < 1:1458544/134‑1 (MQ=255) attcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATACAGCTTGAGTGGAGTACAGCGACGC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt < 1:2101001/134‑1 (MQ=17) cagatAGAGATTCGGACTCATACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc < 1:2541576/134‑1 (MQ=255) gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCga > 2:2397249/1‑134 (MQ=255) gagaGCGAGTCGTACTAGGATAACGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTC‑‑‑AGATTCAGATATCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga < 2:1972968/131‑1 (MQ=14) atAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACGCAGATAGCgac > 2:2246684/1‑134 (MQ=255) tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgact < 1:1375324/134‑1 (MQ=255) tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATGCAGACAGCGATTCAGACTCAACTAGCaaca > 2:277868/1‑133 (MQ=255) tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCAACTC‑‑‑AGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCTATTCAGACTCACATACCgact > 2:509701/1‑134 (MQ=17) aGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGTGACTCTATAGATGCGGA‑‑‑CTACTCAGACACATATAGCGACTCAGAGTCAGACAGCGATTCAGACTCAGATAGCgactc < 1:957966/134‑1 (MQ=2) aGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgcctc > 2:2083756/1‑134 (MQ=255) cGACTCGGACTCAGCGAGCGATTCAGATTCAGATGGCGACTCAGACTCAGACAGCGACTC‑‑‑AGACTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag < 2:92153/134‑1 (MQ=14) aTTCGGACTCAGATAGCGAGTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac < 1:828310/134‑1 (MQ=255) tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCCGACAGCGACTC‑‑‑AGAGTCAGATAGCGATTAGGACTCAGATAGCGACTCAGATAAAGACAGCGATTCAGACTCAGATAGCgactcagactc > 1:565531/1‑134 (MQ=18) tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCaactcagaccc > 2:1451949/1‑132 (MQ=255) tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGAATAAGATAGCGAATAAGATTAAGAAAGAGATTAAGACTCAGATAGAgatttagactc > 1:1070766/1‑134 (MQ=17) cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca < 1:1902909/134‑1 (MQ=255) | GTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTC‑‑‑AGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAG > USA300TCH1516_ALE/622331‑622597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |