Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,536 G→A 5.8% S1033S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5360GA 5.8% 890.4 / 3.8 388S1033S (TCG→TCAsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (170/165);  new base A (29/10);  total (204/184)
Fisher's exact test for biased strand distribution p-value = 6.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 391 total aligned reads displayed.

GCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTG  >  USA300TCH1516_ALE/622403‑622669
                                                                                                                                     |                                                                                                                                     
  gatgcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                     <  2:2534350/130‑1 (MQ=255)
   agtcgtatttagttAGCTATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                                    <  1:2067756/128‑1 (MQ=14)
     tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                  >  1:634218/1‑134 (MQ=255)
     tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                  <  1:2326788/134‑1 (MQ=255)
     tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                  <  2:1511506/134‑1 (MQ=255)
     tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                  >  1:2143217/1‑134 (MQ=255)
     cgagaatcagaaAGCGATTCAGGGTCAGACAGCGACTCAGATTGAGATAGCGATTCAGACACAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                  <  2:1246519/132‑1 (MQ=11)
     acagattcagacAGCGACTGAGATTCAGACAGCGAGTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                  <  2:843691/133‑1 (MQ=11)
      cagattcagagAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                                                                                 <  2:1868515/134‑1 (MQ=255)
       agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                                                                                >  1:2506520/1‑134 (MQ=255)
         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATACCGAGTCGGCCTCAGaa                                                                                                                              >  2:651506/1‑133 (MQ=255)
           tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                            >  1:48200/1‑134 (MQ=255)
            cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                                                                           >  1:778864/1‑134 (MQ=255)
            cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                                                                           <  1:2269808/134‑1 (MQ=255)
            cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                                                                           <  1:1581496/134‑1 (MQ=255)
             aaatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                          <  2:35452/132‑1 (MQ=255)
              gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGCCTCAGATACCGAATCAGACTCAGACAGCGACTCAGGCTCAGATAGCGATTGGGATGCAGAGAGCGa                                                                                                                         >  2:1425599/1‑134 (MQ=14)
              gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                         >  1:1615278/1‑134 (MQ=255)
              gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                         >  2:1806952/1‑134 (MQ=255)
               atAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTAGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                        <  1:1090400/134‑1 (MQ=255)
               atAGCGATGCAGACTCAGACAGCAACTGAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                        <  1:1137669/134‑1 (MQ=255)
               agagagATTCGGAGTCAGAGAGCGATTGCGATTCAGATAGAGATTCGGACTCAGACAGCGATTCAGACTCAGCGAGCGAGTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                        <  2:2847089/129‑1 (MQ=14)
                  gctaTTGAGAGTCAGGAAGCGACTTAGATTTAGATTGCGATTCCGACTCAGACAGCGATTCAGCTTCAGATATCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCa                                                                                                                     <  2:2334285/131‑1 (MQ=14)
                  gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCGGACTCCGCCAGCGACTCa                                                                                                                     >  2:1985910/1‑134 (MQ=14)
                        cagcctcagacAGCGACTCAGATTCAGATAGCTATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCa                                                                                                               <  1:929884/134‑1 (MQ=255)
                          gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                             <  1:2409397/134‑1 (MQ=255)
                           actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                                                                                            >  1:2595011/1‑134 (MQ=255)
                           actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAGTCAGACTCAGATAGCGACtcagactcagac                                                                                                            >  2:104611/1‑134 (MQ=255)
                           actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                            >  2:174765/1‑134 (MQ=255)
                           actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAGAGATTCGGAGTCAGAAAACGACTCAAATTCAGAc                                                                                                            >  2:398843/1‑134 (MQ=14)
                                gacAACGACTAAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                                       <  2:2103555/134‑1 (MQ=14)
                                 acAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGTCTCTGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                                      <  1:67403/134‑1 (MQ=255)
                                   agggACTGAGAGTCAGATAGCGATGCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                    <  2:2004109/131‑1 (MQ=17)
                                   aGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                    >  1:2175900/1‑134 (MQ=255)
                                   aGCGACTCAGATTCAGATAGCGATCCAGATGCAGACTGCGATTCTTACTCAGATAGCGACTCAGACTCAGACATCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                    <  2:319514/134‑1 (MQ=14)
                                   aGCGAATCCGATTCAGAAAGCGATTCAGACTCAGACAGCGATTCAGGCTCAGATAGCGACTCAGACTCAGACATTGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                    <  1:732962/134‑1 (MQ=14)
                                     ctaCTCAGATTCAGATAGCGATTCTGATTCAGACAGCGATTCAGACTCAGATAGCGACGCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                  <  1:1267868/132‑1 (MQ=255)
                                      tgcTCAGTTGCAGAAAGCGAGTCGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                                 <  2:195960/132‑1 (MQ=14)
                                      gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                                 <  1:589403/134‑1 (MQ=255)
                                      gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                                 <  1:1319401/134‑1 (MQ=255)
                                       aCTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                                <  1:289145/134‑1 (MQ=255)
                                        cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCCGATAGCGATTCGGACTCAGATAGCGATTCAGATTCAGACAGCGATTCAGACt                                                                                               >  2:146574/1‑134 (MQ=255)
                                         cgagactcaggtTGCGATTCGAAATAGGTCATCGATTCAGACTCAGATAGCGACTCGGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                              <  2:2766837/132‑1 (MQ=17)
                                         acagatgcagatAACGATTGAGACTCATACAGCGATTCAGACTCAGAGAGCGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                              <  2:1366494/133‑1 (MQ=11)
                                           agattcagatAGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGACTTATACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                                            <  1:1001207/134‑1 (MQ=255)
                                           agattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                                            <  1:999916/134‑1 (MQ=255)
                                            gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                           >  1:709346/1‑134 (MQ=255)
                                             attcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCATACTCTGTCTGCTACTCAGATTCAGATAGCGATTCGGACTCATAGAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                          <  1:398511/134‑1 (MQ=17)
                                               tcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAACGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                        >  1:826664/1‑134 (MQ=255)
                                                cagatAGAGATTCGGACTCATACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc                                                                                       <  1:2541576/134‑1 (MQ=255)
                                                  gatCGCGACTCAGACTCAGACAGCGATTCAGACTCAGTTAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGTCAGCGATTCAGACTCAGATAGCga                                                                                     <  2:910712/134‑1 (MQ=17)
                                                    tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCAACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCTATTCAGACTCACATACCgact                                                                                   >  2:509701/1‑134 (MQ=17)
                                                       cGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGGTTCAGAAAGCAATTCGGCGGCGAAAACCAACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                <  1:545700/134‑1 (MQ=17)
                                                       cGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                >  1:1299947/1‑134 (MQ=255)
                                                            cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                           <  1:464801/134‑1 (MQ=255)
                                                            cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                           <  1:1902909/134‑1 (MQ=255)
                                                              gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                         <  1:1344033/134‑1 (MQ=255)
                                                               aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGAGATTCAGACTCAGATAGCGACtcagactcagac                                                                        >  2:1089353/1‑134 (MQ=255)
                                                                 tCAGATAGAGATGCAGACTCACATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                      <  2:2601480/134‑1 (MQ=255)
                                                                 tCAGACAGCGAGTAAGATTCAGATGGCGACGCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                      <  2:2571842/134‑1 (MQ=14)
                                                                    tataGCGATTCACACTCTGATTGTGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                   <  2:62673/131‑1 (MQ=14)
                                                                        gCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattca                                                               >  1:1408957/1‑134 (MQ=255)
                                                                         cGATTCAGTATCAGATAGCGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACGCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag                                                              <  1:2697114/134‑1 (MQ=255)
                                                                         cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATAAGACAACAATACAGACTCAAATAGCGACTCAGACTCAGACAGTaattcag                                                              >  1:854145/1‑134 (MQ=17)
                                                                          gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                             <  2:2670209/134‑1 (MQ=255)
                                                                              cagatgcAGATAGCTACTCAGATGCAGACAGCGACGAAGAATCAGAAAGCGATTGGGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattca                                                         <  2:190524/128‑1 (MQ=14)
                                                                              cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattca                                                         <  1:1838999/134‑1 (MQ=255)
                                                                              cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAAACAGTgaatcagataca                                                         >  2:1207661/1‑134 (MQ=255)
                                                                                gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAAACAGTGAttcagattcaga                                                       >  1:286102/1‑134 (MQ=255)
                                                                                gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGAGACTCAGACTCAGACAGTGAttcagagacaga                                                       >  2:1675589/1‑134 (MQ=255)
                                                                                 aCTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACACAGACAGTGAttcagattcagaa                                                      >  1:1022751/1‑133 (MQ=255)
                                                                                 aCTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAACGACTCAGACTCAGACAGAGATTCAGACACAGAc                                                      >  2:789328/1‑134 (MQ=255)
                                                                                        tatcGACTCAGACTCAAAAATCGACTAAGATTTTGATATTTATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgact                                               <  1:305897/131‑1 (MQ=14)
                                                                                         aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                              >  1:1651470/1‑134 (MQ=255)
                                                                                         aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACACAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                              >  1:2096893/1‑134 (MQ=255)
                                                                                            gactcagactcaGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCAACTCAGATTCATACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcaga                                           >  1:406463/1‑134 (MQ=255)
                                                                                             actcagactcagACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGCTACCAACAGCgactcagac                                          >  2:2418333/1‑134 (MQ=255)
                                                                                             actcagactcagACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagac                                          <  1:332408/134‑1 (MQ=255)
                                                                                               tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactc                                        >  1:2207691/1‑134 (MQ=255)
                                                                                                   actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCAATTCAGACACAGATAGAAACTCAAACACAGACAGAGATTCAGATTCAGACAGCGACTCAGACTCAGAt                                    >  1:1163999/1‑134 (MQ=17)
                                                                                                    ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACCGCGCTTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATa                                   >  1:2514379/1‑134 (MQ=255)
                                                                                                       agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCg                                >  2:551383/1‑134 (MQ=255)
                                                                                                        gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCCGACAGCGACTCAGACTCAGACAGcca                               >  2:1601208/1‑132 (MQ=255)
                                                                                                        aacAGCTACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGa                               <  1:1207334/133‑1 (MQ=255)
                                                                                                          cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGATTCAGATTCAGACAGCGACTCAGACTCAGATAACGACt                             >  2:2223189/1‑134 (MQ=255)
                                                                                                          cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACt                             >  1:2766509/1‑134 (MQ=255)
                                                                                                                cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAtt                       >  1:22481/1‑134 (MQ=255)
                                                                                                                  cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCATACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCg                     <  1:345319/134‑1 (MQ=255)
                                                                                                                        ccgatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATACAGACAGCGACTCAAACTCAGATAGCGACTCAGATTCGGACAGc               >  1:968061/3‑134 (MQ=255)
                                                                                                                          gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGa             >  1:1456756/1‑134 (MQ=255)
                                                                                                                            tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGAGACTCAAACTCAGATAGCGACACAAAATCAAACAGCGACt           >  1:2486714/1‑134 (MQ=14)
                                                                                                                             aGCGATTCGGACTCAAATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTc          <  1:1796887/134‑1 (MQ=255)
                                                                                                                             aGCGAGTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTc          <  1:1434942/134‑1 (MQ=255)
                                                                                                                              gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                           <  2:1136000/52‑1 (MQ=255)
                                                                                                                               cGACTCGGACTCCGATAGCGACTCAGATTCATACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAg        <  1:2633065/134‑1 (MQ=255)
                                                                                                                                   tCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAACGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc    >  1:1715357/1‑134 (MQ=255)
                                                                                                                                   tCGGACTCAGACAGCGTTTCAGATTCAAACCGCGACTCAGACTCAGATAGCGTCTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc    <  1:1955746/134‑1 (MQ=17)
                                                                                                                                    cGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCGGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGAAAACGACTCAGATTCGGACAGCGACTCAGActct   >  1:201485/1‑134 (MQ=255)
                                                                                                                                    cGGACGCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActct   <  1:791413/134‑1 (MQ=255)
                                                                                                                                     ggACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACACCGACTCAGACACTg  >  2:1272343/1‑134 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTG  >  USA300TCH1516_ALE/622403‑622669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: