Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,324,247 C→A 6.1% P340Q (CCA→CAA)  mutL → DNA mismatch repair protein MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,324,2470CA 6.1% 476.0 / 2.7 205P340Q (CCA→CAA) mutLDNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base C (104/80);  new base A (6/13);  total (111/94)
Fisher's exact test for biased strand distribution p-value = 5.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 212 total aligned reads displayed.

GTAAATGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGGAAGAAAAT  >  USA300TCH1516_ALE/1324114‑1324380
                                                                                                                                     |                                                                                                                                        
gTAAATGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGcc                                                                                                                                          <  1:1835981/134‑1 (MQ=255)
gTAAAGGTTAATCCAAAAAAAGTAGAAGTGGGTGTCGCTAAAAAAGAGAAAGTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGcc                                                                                                                                          <  2:2778912/134‑1 (MQ=255)
 tAAATGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAAATTGGATTATGTGCCa                                                                                                                                        >  1:1262638/1‑134 (MQ=255)
  aaaTGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAATTATATCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCaa                                                                                                                                       <  1:2744822/134‑1 (MQ=255)
  aaaTGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCac                                                                                                                                       >  2:2340842/1‑133 (MQ=255)
   aaTGTTCATCCAACAAAACTAGAAGTGCTTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACATGGATTATGTGCCaaa                                                                                                                                      >  1:2764489/1‑134 (MQ=255)
   aaTGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACATGGAATATGTGACaaa                                                                                                                                      >  1:2062580/1‑134 (MQ=255)
   aaTGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTAAACAAACGTCTAAAAATTCCTAAAAATAACTTGGATTATGTGCCaaa                                                                                                                                      >  1:532679/1‑134 (MQ=255)
   aaTGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGAATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCaaaa                                                                                                                                      >  1:721189/1‑134 (MQ=255)
    aTGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCaaaa                                                                                                                                     >  2:2234422/1‑134 (MQ=255)
     tGTTCATCCAACAAAACTAGAAGTGCGTTTATAAAAAGAAGAGCAATTATTCTTTTTGTATGAGAGCAAAATACAAGAAACATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCaaaaaa                                                                                                                                    <  1:255125/134‑1 (MQ=255)
      gTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCaaaaaa                                                                                                                                   >  1:2238206/1‑134 (MQ=255)
       ttcagacaacaaAACTAGAAGTGCGTTTATCAAAAGAAGAGAAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAACACCGTATATTAATTCCTAAACATAACTTGGATTATGTaaaaaaaaaa                                                                                                                                  <  2:1556891/128‑1 (MQ=255)
        tCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGAAACTATACCAATTTATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCaaaaaaaa                                                                                                                                 <  1:1179438/134‑1 (MQ=255)
         cATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTAATAGTAAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACATGGATTATGTGCCaaaaaaaaa                                                                                                                                >  1:893255/1‑134 (MQ=255)
         cATCCAACAAAACAAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTTGAATATGTGCCaaaaaaaaa                                                                                                                                >  2:655056/1‑134 (MQ=255)
            cctacaaAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAACAAATaa                                                                                                                            >  2:2674930/4‑134 (MQ=255)
               acaaAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATATCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGt                                                                                                                         <  1:2590701/134‑1 (MQ=255)
               acaaAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTTATTGTGAGAAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATATCTTGGATTATGTGCCAAAAAAAAATAAAGt                                                                                                                         <  1:2331772/134‑1 (MQ=255)
                caaAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGAGCCAAAAAAAAATAAAGTa                                                                                                                        >  2:2055480/1‑134 (MQ=255)
                    aCTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGAATAAATGCAAAAAAAAAAAAAAGAATTAc                                                                                                                    >  1:2465905/1‑134 (MQ=255)
                    aCTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAAAACCTAAAAATAACTAGAATAATATGCAAAAAAAAAAAAAAgaaataa                                                                                                                    >  1:1155055/1‑128 (MQ=255)
                    aCTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGAACGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGAATTAc                                                                                                                    >  1:1917759/1‑134 (MQ=255)
                       gaaaGAGCGTTTATAAAAAGAATAGCTATTATACTAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTACTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTAcatt                                                                                                                 <  1:2526804/132‑1 (MQ=255)
                       aGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTAcatt                                                                                                                 >  1:2751096/1‑134 (MQ=255)
                       aGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATACCAAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTAcatt                                                                                                                 >  1:576523/1‑134 (MQ=255)
                       aGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAACTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTACCAAAAAAAAAAAAAATATAAaatt                                                                                                                 >  2:2440175/1‑134 (MQ=255)
                                     cAAAATAAGAGCAACTATACATATTGATTGTGAAGAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAAcaacaa                                                                                                  <  2:283192/134‑1 (MQ=255)
                                           aagaGCAACTATACCAATTGATTGTGAGCAAAATACAATAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATc                                                                                            <  1:154874/134‑1 (MQ=255)
                                           aagaGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATc                                                                                            <  1:2133926/134‑1 (MQ=255)
                                           aaaagCAACTAAACCAAATGAGTGTGGGACCAATTCACAGAGGGTTAAAAGACCGTATAGTAATTCCTAAAAATAACTTGGATTATGAGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATc                                                                                            <  2:2030214/131‑1 (MQ=255)
                                            agagCAACTATATCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCg                                                                                           <  1:2630712/134‑1 (MQ=255)
                                            agagCAACTATACGAAGTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCg                                                                                           <  2:1967867/134‑1 (MQ=255)
                                             gagCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGTATTAAAAGACCGTATATTAATTCCTAAAAATAAATTGGATTAAGTGCCAAAAAAAATAAAGTAATACATTCATTAGAACCAAAAAAAATCaaa                                                                                         >  2:1448069/1‑134 (MQ=255)
                                               gCAAGTATAGCAAGAGATTATGAGCAAAATACAAGAAGCATGTAAAGACCGTATATTAATTCATAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAt                                                                                        <  2:1053292/134‑1 (MQ=255)
                                               gCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAt                                                                                        <  1:2785240/134‑1 (MQ=255)
                                               gCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATATGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAt                                                                                        >  1:1003464/1‑134 (MQ=255)
                                               gCAAATATAGCAATTGATTGTGAGAAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAAATTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAt                                                                                        <  2:554374/134‑1 (MQ=255)
                                                cAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGTCCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAtt                                                                                       <  1:179931/134‑1 (MQ=255)
                                                 aaCTATACCAATTGATTGTGAGCAAAATACAAGAAGCACTTAAAGACCGTATATTAATACCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGGATTACATTCAATCGAACAAAACAAAAACGAAtgt                                                                                      >  2:905731/1‑132 (MQ=255)
                                                  aatataACAATAGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTg                                                                                     <  2:326548/132‑1 (MQ=255)
                                                      taCTAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGAATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACa                                                                                 <  1:962891/134‑1 (MQ=255)
                                                      taCCAATTGATTGTGAGCAAAATACAATAAGCATTTAATGACCTTAAATTTCTTTCTGATAATAACTGTGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACa                                                                                 <  1:2849658/134‑1 (MQ=255)
                                                        ccAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCAGTCGAACAACAAAAAATAGAATTTGaaaaaa                                                                               >  1:783902/1‑134 (MQ=255)
                                                         cAATTGATTGTGAGCAAAATACAAGAATCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAg                                                                              <  1:975685/134‑1 (MQ=255)
                                                         cAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGAATTACATTCAATCGAACAACAAAAAATCGAATTTGAACAAAg                                                                              >  2:1203598/1‑134 (MQ=255)
                                                          aaTTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGa                                                                             >  2:2362950/1‑134 (MQ=255)
                                                           attgattgTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTAGGATTATGTGCCAAAAAAAAACTTTTGAAAGAAAGACATTAACAAAACAAAAAATCGAATTTGAACAAAGa                                                                             >  1:1341906/1‑134 (MQ=255)
                                                            ttgattgTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACa                                                                           <  1:111770/134‑1 (MQ=255)
                                                            ttgattgTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCAAAAAAAAAATAAAGTATTACATTCATTCGAACAAAAAAAAATCAAATTTGAACAAAGAcc                                                                           >  2:2307626/1‑133 (MQ=255)
                                                              gattgTGAGCAAAATACAAGAAGCATTTAAATACCGTATATTAATTCCTAATAATAACTTTGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAACCGAATTTGAACAAAGACaaaa                                                                        <  1:1418427/134‑1 (MQ=255)
                                                                    gAGCAAAATACAAGAAACATTTAAAGACCGTATATTAATTCCTAAAAAAAACTTGGATTATGTGCCAAAAAAAAATAAAGAATTACATACATTCGAACAACAAAAAATAGAATTTAAacaaaaacaaaaaaaag                                                                   >  1:846222/1‑129 (MQ=255)
                                                                      gCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACagag                                                                 <  2:2170256/134‑1 (MQ=255)
                                                                      gCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAAGCGAATTTGAACAAAGACAAAAcacaaag                                                                 <  1:181277/134‑4 (MQ=255)
                                                                      gCAAAATACAAGAAGCAATTAAAGACAGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACagag                                                                 <  1:1139358/134‑1 (MQ=255)
                                                                       gaaaaGACAAGAAGCATTTAAAGAGCGTATATTAATTCCTAGAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAgaga                                                                <  2:830243/133‑1 (MQ=255)
                                                                         aaaaACGAGAAGAATTAAAACACAGAATAATACTTCATAAAAATAACTTGGATAAAAAAAAAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAAt                                                              <  2:117130/134‑1 (MQ=255)
                                                                         aaaTACAAGAAACATTTAAAGACCGTATATTAATTCCTAAAAATAACATGGATTATGAGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAAt                                                              <  2:2329336/134‑1 (MQ=255)
                                                                               aagaagCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAACAAAAAAAAAAAAATTACATTCATAAGAACAACAAAAAATCGAATATGAACAAAGACAAAACACAGAGAATAACCaa                                                        >  1:407524/1‑134 (MQ=255)
                                                                                agaagCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATAAAAACAACAAAAAAACAAATCTGAACAAAAACAAAACACAGAGAATAACCAag                                                       >  1:1478039/1‑134 (MQ=255)
                                                                                 gaagCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAaga                                                      <  2:1235676/134‑1 (MQ=255)
                                                                                     cATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCTAAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGagaa                                                   <  2:535892/134‑1 (MQ=255)
                                                                                       tttAAAGACCTTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGAc                                                <  1:2047322/134‑1 (MQ=255)
                                                                                        gtAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGACAAAAAAAAAGAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACg                                               <  2:2660992/133‑1 (MQ=255)
                                                                                          aaaGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGACTCTGAACAAAAAAAAAAAAAAAAAAATAAAAAAAAGAAAACGtt                                             >  1:1083710/1‑134 (MQ=255)
                                                                                          aaaGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAAAAAAAAGAATAAGAAGGAGAAGAcaat                                             >  1:1071472/1‑131 (MQ=255)
                                                                                            aGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATAAAATAAGAAAAAAAACAAAACAAAGAGAATAACCAAGAGAAAACGtttt                                           >  1:1620382/1‑134 (MQ=255)
                                                                                             gACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAAAAAAGACAAAAAAAAGAGAATAAAAAAGAGAAGACGTTTTc                                          >  1:1722828/1‑134 (MQ=255)
                                                                                               tcGTATATTAATTCCTAAAAATAACTTGAATTAAGTACAAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCTAGAGAAGACGTTTTCAt                                        <  2:402785/133‑1 (MQ=255)
                                                                                                cGTATAATAAGTACGAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATc                                       <  2:2460575/134‑1 (MQ=255)
                                                                                                 gTATATTAATTCACAAAAATAACTACAATTATTTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTg                                     <  2:1393969/134‑1 (MQ=255)
                                                                                                   atatTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTga                                    <  1:905731/134‑1 (MQ=255)
                                                                                                    tatTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTgaa                                   <  2:512395/134‑1 (MQ=255)
                                                                                                    tatTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATATgaa                                   >  1:2662960/1‑134 (MQ=255)
                                                                                                     atTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTgaag                                  <  1:2195738/134‑1 (MQ=255)
                                                                                                     atTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTgaag                                  >  2:1082505/1‑134 (MQ=255)
                                                                                                     atTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAAAATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAAACAAAACACAGAAAATAACAAAGAGAAGACGCTTTAAtacaaag                                  >  2:2258804/1‑128 (MQ=255)
                                                                                                         aTTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAg                              <  1:2307626/134‑1 (MQ=255)
                                                                                                          ttCCTAAAAATAACTTGTATTACGTAGCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGt                             <  2:1513704/134‑1 (MQ=255)
                                                                                                          ttCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAATTGAACAAAAACAAAACAAAGAGAATAACAAAGAGAAGACGATATCATCTGAAGaaaat                             >  2:2221699/1‑132 (MQ=255)
                                                                                                           tCCTATAAATTACTTCGATTATATACCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAAGCGAATTTGAACAAAGACAAAACACTGAGATTAACCAAGAGAAGACGTTTGCATCTGAAGAAAGTa                            <  2:396722/134‑1 (MQ=255)
                                                                                                            ccTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTaa                           <  1:193346/134‑1 (MQ=255)
                                                                                                             cTAAAAATAACTTGGATTATGTGCAAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAAc                          <  2:2714894/134‑1 (MQ=255)
                                                                                                              tAAAAATAACTTGGATTATGTGCCAAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAAc                          <  2:1341906/134‑1 (MQ=255)
                                                                                                              tAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGCAAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAAAAAGAAACa                         >  2:362484/1‑134 (MQ=255)
                                                                                                              tAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACa                         <  1:2220841/134‑1 (MQ=255)
                                                                                                                  aaTAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATAAGAAAAAAAAAAAAACGAAATTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTaa                     >  1:157647/1‑134 (MQ=255)
                                                                                                                  aaTAACTTGGAATATGAGACAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTaa                     <  1:1977001/134‑1 (MQ=255)
                                                                                                                   aTAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAATACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAg                    <  1:381860/134‑1 (MQ=255)
                                                                                                                     aaCTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACAAAGAGAATAACAAAGAGAAGACGTTTTCAAATGAAGAAAGTAACAGTAAGcc                  >  1:1636030/1‑134 (MQ=255)
                                                                                                                     aaCTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAACTTGAACAAAGACAAAACACAGAGAATAAAAAAAAGAAGAAGTTTTCATCTGAAGAAAGTAACAGTAAGcc                  >  1:853623/1‑134 (MQ=255)
                                                                                                                      aCTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCa                 >  1:2487036/1‑134 (MQ=255)
                                                                                                                      aCTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCAAATTTGAACAAAAACAAAACACAGAGAATAACAAAGAAAAAACATTTTCATACGATGAAAGTAAAAGTAAGcaa                 >  2:1873524/1‑132 (MQ=255)
                                                                                                                      aCTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCAAACAACAAAAAATCCAATTTGAACAAAGACAAAACACAGAAAAAAACCAAGAGAAGACATATTCAACAAAAAAAAGTAACAGTAAGCCa                 >  2:2592247/1‑134 (MQ=255)
                                                                                                                       cTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGTATTTGTACAAAGACAAAACACAGAGAATAACCAAAAGAAGACGTTTTAATCTGAAGAAAGTAAAAGTAAGCCAt                >  1:482268/1‑134 (MQ=255)
                                                                                                                       cTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCAt                >  2:1260471/1‑134 (MQ=255)
                                                                                                                         tGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTAGAACAAAAAAAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGAAACAGTAAGCCAtat              >  1:1402607/1‑132 (MQ=255)
                                                                                                                              tATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAAAGAAGACGTTTTCAACTGAAAAAAGAAAAAATAAGCCAttaaagca         >  2:2088825/1‑128 (MQ=255)
                                                                                                                                tgtgCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCAGAAGAAAGTAACAGTAAGCAATTTATGgaa        >  2:1688787/1‑134 (MQ=255)
                                                                                                                                  tgCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGgaagaa     >  2:1571483/1‑134 (MQ=255)
                                                                                                                                     |                                                                                                                                        
GTAAATGTTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGGAAGAAAAT  >  USA300TCH1516_ALE/1324114‑1324380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: