BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,857,785	A→T	8.4%	S197T (TCA→ACA)	USA300HOU_RS09235 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,857,785	0	A	T	8.4%	595.8 / 5.2	303	S197T (TCA→ACA)	USA300HOU_RS09235	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (131/132); new base T (8/25); total (141/162)
Fisher's exact test for biased strand distribution p-value = 5.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

GCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAAT  >  USA300TCH1516_ALE/1857652‑1857918

gCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGa                                                                                                                                       <  2:863768/134‑1 (MQ=255)

  ttGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTACATTATTTGCACTTCCTGCTTCTTTTTCCGGCTCGTAGTGTGCTTTCAATGCCAT‑TGCTTTCGGTTCTTCATTTGATa                                                                                                                                     <  2:2794785/134‑1 (MQ=14)

  ttGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACGTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATa                                                                                                                                     <  2:1968266/134‑1 (MQ=255)

    gtgCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCACTCCCCC‑TGCTTTTTGTTCTTTATTTGATata                                                                                                                                   >  2:2121919/1‑132 (MQ=11)

     tgCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAg                                                                                                                                  >  1:2414385/1‑134 (MQ=255)

     tgCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAg                                                                                                                                  <  2:1387419/134‑1 (MQ=255)

      gCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGATTCTTTATTTGATACAGc                                                                                                                                 >  2:2268714/1‑134 (MQ=17)

      gCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTGGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGc                                                                                                                                 >  2:1578548/1‑134 (MQ=255)

        ttcttTTTTAGCTTCTTGATCCAGTCGTGCGTCTTGTTATATATCACTTAAATGATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTGGTGCTTTCAATTCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTg                                                                                                                               <  2:161270/134‑1 (MQ=14)

        ttcttTTTTAGCTTCTTGAACATCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTg                                                                                                                               <  1:294009/134‑1 (MQ=255)

         tcgtttttAGCTTCTTGAACCTGTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGc                                                                                                                              <  2:1017518/131‑1 (MQ=255)

          cttTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTTATTTGATACAGCTGCa                                                                                                                             >  1:1301914/1‑134 (MQ=255)

          cttTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCa                                                                                                                             <  1:2340983/134‑1 (MQ=255)

             ttttAGCTTCTTGAACCTCTTGTGCCTCTTTTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACtt                                                                                                                          <  2:1110939/134‑1 (MQ=255)

              tttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTATAGAGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACttt                                                                                                                         >  1:943397/1‑134 (MQ=255)

                tAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTa                                                                                                                       >  1:1899886/1‑134 (MQ=255)

                 aGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTAt                                                                                                                      >  1:1719540/1‑134 (MQ=255)

                 aGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTAt                                                                                                                      >  1:2371742/1‑134 (MQ=255)

                  gCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCTCTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATc                                                                                                                     <  1:1681864/134‑1 (MQ=255)

                  gCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTTTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATc                                                                                                                     <  1:305994/134‑1 (MQ=255)

                   cttcttGGGCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTGTTATCGCTGCTTGTTGTGCTTTCCATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCt                                                                                                                    <  2:1324331/134‑1 (MQ=255)

                   cttcttGAACCTCTTGTGCCTCTTGTGATATATCACTTATATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGTTTATTCATTTGCTACAGATTCAATTTTATCt                                                                                                                    >  1:384474/1‑134 (MQ=14)

                   cttcttGAACCTCTTGTGCCTCTTGTGATATATCACTTATATTATTTGCACTTGCTTCTTCTTTAATTGCTTCTGTTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCt                                                                                                                    <  2:185781/134‑1 (MQ=17)

                    ttcttGAATCTCTTGTGCCTCATGTGCTATATCACTTAAATGAATGGGACTTGCTTCTTCTTTGAGCGCTGCTTGTTGTGCTTTCAGTGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTg                                                                                                                   <  1:1076412/134‑1 (MQ=14)

                     tcttGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGt                                                                                                                  <  1:222158/134‑1 (MQ=255)

                        tGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTc                                                                                                               <  1:2040172/134‑1 (MQ=255)

                           aCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCTCTGCTTGTTGTGCTTTCACTGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCCACCCTTTTATCTGCTTCTGc                                                                                                            >  2:255085/1‑134 (MQ=11)

                           aCCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCATTTGATTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGc                                                                                                            >  1:143640/1‑134 (MQ=255)

                            gctcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCCGCt                                                                                                           <  2:2815339/133‑1 (MQ=255)

                            cctcttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCt                                                                                                           >  1:2154047/1‑134 (MQ=255)

                               cttgtgccttttgtgATATATCACTTAAATTATTTGCACTTGTTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTtgt                                                                                                        <  2:218157/134‑1 (MQ=255)

                               cttgtgcctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCTCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTtgt                                                                                                        <  1:194131/134‑1 (MQ=255)

                                ttgtgcctcttgtgATATATCACTTAAATAATTTGCACTTGCTTCTTCGTTTATCGCTGCTTGTTGTGCTTTCAATGCCACAGGCTGATCG‑TCTTCATTTTATACAGCAACACCTATACCTGATTCTGCTtgag                                                                                                       >  2:471985/1‑132 (MQ=2)

                                     cctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCTATGCCAC‑TGCTTTTGGTTCTTCATTTGTTACAGCTGCACTTTTATCTGTTTCTGCTTGTGcttct                                                                                                  <  2:341998/134‑1 (MQ=255)

                                     cctcttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTTATTTGATACAGCTACACTTTTATCTGTTTCTGCTTGTGcttct                                                                                                  >  2:749634/1‑134 (MQ=255)

                                       tcttttgATATATTACTTAAATTATTTGCTCTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttt                                                                                                <  1:2620325/134‑1 (MQ=255)

                                         ttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttttt                                                                                              <  1:2436370/134‑1 (MQ=255)

                                         ttgtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCttttt                                                                                              >  1:1413685/1‑134 (MQ=255)

                                           gtgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGTTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTa                                                                                            <  1:974002/134‑1 (MQ=255)

                                             gATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTAATTTTATACAGCTGCACCTTTATCTTTTTTTGCTTGTACTTCCATTTCAGc                                                                                          >  2:2441980/1‑134 (MQ=17)

                                                atatCACTTAAATTATTTGCTCTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGcttc                                                                                       <  1:1162281/134‑1 (MQ=255)

                                                atatCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTTAATGCCAT‑TGCTTTTTGTTCTTTATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGcttc                                                                                       <  1:713623/134‑1 (MQ=255)

                                                 tatCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTTAATGCCAC‑TGCTTTGGGTGCGGCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGcttct                                                                                      <  1:1038499/134‑1 (MQ=17)

                                                  atCACTTAATTTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGcttctt                                                                                     <  1:2537570/134‑1 (MQ=255)

                                                   tCATTTCAATTTTTTGCACTTGCTTCTTTTTTTATCGCTGCTTGTTGTGCTTTTACTGCCAC‑TGCTTTTGGTTCTTCATTTGTTACAGCTGCACTTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTg                                                                                    <  2:962210/134‑1 (MQ=17)

                                                        tAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCCTCTGTTTCTTCTTTTGTTACAACTTCTCTATTATCTATTTGTGCCTGGTCTGTTTTTTTTGTTTCTTGAAttt                                                                               >  2:2844926/1‑132 (MQ=9)

                                                         aaaTTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGTTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAAtctc                                                                              <  1:918925/134‑1 (MQ=255)

                                                          tattattTGCACTTGCTTCTTCTTTTATTGCTGCTTGTTGTGCTTTTAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATctct                                                                             <  2:943107/133‑1 (MQ=255)

                                                            ttattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGTTTTTGGTTCTTAATTTGTTACAGCTTCACTTTTCTCTGTTTCTTCTTATGCTTATCTTTTACCTTCTTTAATCtttta                                                                           >  2:1531010/1‑133 (MQ=11)

                                                             tgtttGTATTTGCTTCTTCGTCTATCGGTGCTGCTGGTGCTTTCAATGCCAC‑TGTTGTTGGTGTTTCATGTGATACAGCTGCTTTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTtgt                                                                          <  2:250426/132‑1 (MQ=17)

                                                             tattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGATTCTTCATTTGATACAGCTGCACTTTTATCCGTTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTtgt                                                                          >  2:563398/1‑134 (MQ=12)

                                                              attTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCTAC‑TGTTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTtgtg                                                                         <  2:2213819/134‑1 (MQ=255)

                                                               tttGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTTAATGCCAC‑TTCTTTTTGTTCTTCTTTTTATAAAACTGCACCTTTACCTATTTCTGCATATGCTCCTTTATTAGCTTCTTGTATCCCTtgttc                                                                        >  2:508459/1‑132 (MQ=17)

                                                               tttGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGc                                                                        <  1:2824099/134‑1 (MQ=255)

                                                                ttGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGcc                                                                       <  1:1531010/134‑1 (MQ=255)

                                                                 tGCGCTTGCTTATTCTTTTATCGCTGCTTGTTGTTTTTTTAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACTGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCct                                                                      <  2:1684079/134‑1 (MQ=17)

                                                                 tGCAGTTGCTTCTTCTTTTGTCGTTGCTTTTTGTGTTTTTACTGCCAC‑TGCTTTTGTTTCTTCATTTGTTACTGCTGCACTTCTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCct                                                                      <  2:2753825/134‑1 (MQ=17)

                                                                  gCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCctc                                                                     <  1:1499626/134‑1 (MQ=255)

                                                                      ttGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTCCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTtgt                                                                 <  1:2257557/134‑1 (MQ=255)

                                                                         cttcttcttTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGTTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTTTGCCTCtttttat                                                              >  2:1194477/1‑129 (MQ=255)

                                                                           tcttcttTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGt                                                            >  1:1734077/1‑134 (MQ=255)

                                                                                 tttATCGTTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTTTTTGTACCGCACAACTGCAGTTTTATCGTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACt                                                      <  1:1234536/134‑1 (MQ=14)

                                                                                 tttATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGATTCTCTTGTGCGTCTTGTTATGTAtttgt                                                      >  2:1704639/1‑130 (MQ=255)

                                                                                  ttATCGCTGCTTGTTGTGCTTTCAATTCCAC‑TGCTTTTTGTTCTTCATTTGATACAGCTGAACTTATCTAATATCCGTATAGTGCTGTTCTGGTGTGTTTGTGAATCTCTTGTGCCTCTTGTGATGTATCACTc                                                     <  1:678077/134‑1 (MQ=9)

                                                                                   tATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTAGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTCCTTGCGCTTCTCTTTTAACTACTTGAAACTCTTATGCCAATTGTGATGTAACACTCa                                                    >  1:471115/1‑134 (MQ=17)

                                                                                      cGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGGGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAat                                                 <  1:270762/134‑1 (MQ=255)

                                                                                       gctgctTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCGTCTTGTGATGTATGACTCAAatt                                                >  2:1234066/1‑134 (MQ=255)

                                                                                        ctgctTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTTCTTCTTTTGATGTATCATTTAAataa                                               >  2:910894/1‑132 (MQ=14)

                                                                                         tgctTGTTGTGCTTTCAATGCCAC‑TGCTTTTAGGTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTACCTCTTGTGATGTATCACTCAtattat                                              >  2:201390/1‑134 (MQ=255)

                                                                                          gctTGTTGTGCTTTCAATTCCAC‑TGCTTTTGGTTCTTCATTTTATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAattatt                                             <  1:510384/134‑1 (MQ=255)

                                                                                          gctTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACTGCTGTACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAattatt                                             <  1:823014/134‑1 (MQ=255)

                                                                                             tgttgtGCTTTCAATTCCTC‑TGCTTTTGGTTCTTCATTTTATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGc                                          <  1:1635611/134‑1 (MQ=255)

                                                                                             tgttgtGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGc                                          <  1:2670491/134‑1 (MQ=255)

                                                                                               ttgtGCTTTCAATTCTAC‑TTCTTTTGGTTCTTCATTTGTTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCAc                                        <  2:2062589/134‑1 (MQ=255)

                                                                                               ttgtGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATTTGTTTCTGCTGGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCAc                                        <  1:795792/134‑1 (MQ=255)

                                                                                                tgtgCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACTGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACt                                       <  1:2314314/134‑1 (MQ=255)

                                                                                                 gtgCTGTCAATTCCAC‑TGCTTTTGGTGTGTCATTTGATGTAGCTGCATTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACtt                                      <  1:2490945/134‑1 (MQ=17)

                                                                                                 ctgCTTTCAATGCCAC‑TGCTTTTGGTTATTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTAAAATTATTTGCACtt                                      >  2:1777837/2‑134 (MQ=255)

                                                                                                   gCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGc                                    <  1:1578548/134‑1 (MQ=255)

                                                                                                    tcttCATTGCCAC‑TGCTGTTGGTTCGTCATTTTTTACAGCTGCATTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTTTTCGTCTTGTGATGTATCACTCAAATTATTTGCACTTGct                                   <  2:1592949/132‑1 (MQ=17)

                                                                                                    cTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGct                                   >  1:2514704/1‑134 (MQ=255)

                                                                                                       tCAATGCCAC‑TGCTTTTGGTTATTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTTCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCTCTTGcttct                                >  2:210371/1‑134 (MQ=255)

                                                                                                           tGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttctt                            <  2:823116/134‑1 (MQ=255)

                                                                                                             ccAC‑TGCTTCTGGTTCTTCATTTGTTGCAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCtttt                          <  2:403282/134‑1 (MQ=255)

                                                                                                              cAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTa                         >  1:1787633/1‑134 (MQ=255)

                                                                                                              cAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATAACTCAAATTATTTGCACTTGCTTCTTCTTTTa                         >  1:2648524/1‑134 (MQ=255)

                                                                                                                 cttcTTCTGGTTCTTCATTTTATGCTGCTGCATTTTTGTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCCTCTTCTTTTATc                       <  2:1073798/131‑1 (MQ=17)

                                                                                                                  tGCTTTTGATTTTTCATTTGTTATTGCTGTATTTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCg                      <  2:231894/134‑1 (MQ=17)

                                                                                                                   gCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCCCTCAAATTATTTGCACTTGCTTCTTCTTTTTTCgc                     >  2:2603881/1‑134 (MQ=255)

                                                                                                                   gCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTTCTCCTTCTTATATCgc                     >  2:305703/1‑134 (MQ=255)

                                                                                                                    tttttGGTTCTTCATTTTATACAGCTGCACTTTTATCTGTTTCTGCTTGTTTTTTTTTTTTAGTTTCTTGAATCTCTTGTGCCTCTTGGGAGGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCgct                    <  1:21064/133‑1 (MQ=17)

                                                                                                                      tttGGTTCTTCATTTGATTCAGCTACACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTATCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTGAACTTATTTGCACTTGGTTCTTCTGTTATccaggc                  >  2:1449425/1‑129 (MQ=18)

                                                                                                                         ggTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGATTCTTTTTTAGCATCTTGAATCTTTTGTGCCTCTTATGATGTATCAATCTAATTATTTACACTTTTTTCTTTTTTTATCGCTGTttg               >  2:644595/1‑134 (MQ=255)

                                                                                                                          ttttgtcATTTGTTATAGTTGTAACTTTGTTTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTTAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt              <  2:1150038/129‑1 (MQ=14)

                                                                                                                          gTTCTTCATTTGATGCTGATGCACTTTTGTCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt              <  2:2680964/134‑1 (MQ=255)

                                                                                                                            tcttcATTTGTTGCAGCTGCATTTTTGTCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgttg            <  2:784242/134‑1 (MQ=255)

                                                                                                                             cttcATTTTATACAGCTGTACTTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTtgttgt           <  1:592193/134‑1 (MQ=255)

                                                                                                                             cttcATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTtgttgt           >  1:2780694/1‑134 (MQ=255)

                                                                                                                                cATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTATTCTTTTATTGCTGCTTGTTGAGCt        >  2:688855/1‑134 (MQ=255)

                                                                                                                                  tttGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGTCTCTTGTGATGTATCACTCAAATTATTAGCAATTGCTGCTTCTTTTATCGCTGCTTGTTGTGCttt      <  1:120011/134‑1 (MQ=255)

GCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCAC‑TGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAAT  >  USA300TCH1516_ALE/1857652‑1857918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`