BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,028,191	C→A	32.9%	A152S (GCA→TCA)	USA300HOU_RS10230 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,028,191	0	C	A	32.9%	247.1 / 131.9	210	A152S (GCA→TCA)	USA300HOU_RS10230	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (74/61); new base A (37/32); total (113/97)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACC  >  USA300TCH1516_ALE/2028058‑2028324

   ttttCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGc                                                                                                                                    <  1:562324/134‑1 (MQ=255)

   ttttCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGc                                                                                                                                    <  2:1561946/134‑1 (MQ=255)

   ttttCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGc                                                                                                                                    <  2:668129/134‑1 (MQ=255)

       tattcaCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCtttt                                                                                                                                <  2:885851/133‑1 (MQ=255)

       cattcaCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCtttt                                                                                                                                >  1:2698561/1‑134 (MQ=255)

       cattcaCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAAAAACAATTGTATTATTTTACTTAATATTTGCAGAtttc                                                                                                                                >  2:323327/1‑133 (MQ=255)

        atttaCGTAAGTACTTCCATAAAAAGCAGCTACAAGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTg                                                                                                                               <  2:173688/134‑1 (MQ=255)

         ttcaCGTAAGTACTTCCATAAAAAGCAGCTACCTGTAAATTAATATTGTAATTAACCGGCAATATAACAGGAACGTTACGTAGAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTAAAGCTTTTGt                                                                                                                              >  2:28081/1‑134 (MQ=255)

         ttcaCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGt                                                                                                                              >  1:1474387/1‑134 (MQ=255)

          tcaCGTAAGTACTTCCAGAAAAAGCAGCTAAATGTAAATTAATATTGGAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGtt                                                                                                                             <  2:2152306/134‑1 (MQ=255)

           caCGTAAGTACTTCCATAAAAATCAGCTACATTTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTa                                                                                                                            <  1:481717/134‑1 (MQ=255)

           caCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTa                                                                                                                            >  1:2199862/1‑134 (MQ=255)

           caCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGACATTGGCGTAAAAAAAGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTa                                                                                                                            >  1:2849389/1‑134 (MQ=255)

                 aGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCaa                                                                                                                      >  2:1973490/1‑134 (MQ=255)

                  gTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTTCACTTTTCTTAATATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAat                                                                                                                     <  1:774077/134‑1 (MQ=255)

                    aCTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACATAAAAAGAGTCTAACAACAATGGTATTATTTTCCTAAATATTTGCAGCTTTTGTTAAGTCAatat                                                                                                                   >  1:1047709/1‑134 (MQ=255)

                     cTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGACTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATg                                                                                                                  >  1:2403344/1‑134 (MQ=255)

                           tacatagcagcTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTc                                                                                                            <  2:2852857/129‑1 (MQ=255)

                           tAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAACTGTGTCTAACAACAATGCTATTAGTTTCCTTAATATTTGTCGCTTTTGTTAAGTCAATATTTAGATc                                                                                                            >  2:2789392/1‑134 (MQ=255)

                           tAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTc                                                                                                            >  1:2758351/1‑134 (MQ=255)

                             aaaaGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGc                                                                                                          <  2:940117/134‑1 (MQ=255)

                                 gcagcTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAAt                                                                                                      >  1:2447073/1‑134 (MQ=255)

                                   agcTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTc                                                                                                    <  1:1299436/134‑1 (MQ=255)

                                   agcTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCAGCACTTTACCTAAAATATGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTc                                                                                                    >  1:1388365/1‑134 (MQ=255)

                                              aaTTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGTTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAATCAATATGTAGGTCGCAAATTCCATGTTGAAtt                                                                                         >  1:1112625/1‑134 (MQ=255)

                                                  aaTATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTa                                                                                     <  2:2397079/134‑1 (MQ=255)

                                                  aaTATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTATCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTa                                                                                     >  1:59692/1‑134 (MQ=255)

                                                   atatTGTAATTAACCGGCAATATAACCTGCACTGTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTAAATATTTGCAGCTTTTGTTAAGGCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTAc                                                                                    >  1:122787/1‑134 (MQ=255)

                                                    tatTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGTTCGCCAATTCCATGTTGAATTTGTACa                                                                                   >  2:1692186/1‑134 (MQ=255)

                                                    tatTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACa                                                                                   <  2:1988778/134‑1 (MQ=255)

                                                      ttGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATc                                                                                 <  1:1395570/134‑1 (MQ=255)

                                                      ttGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGCCGCCAATTCCATCTTGAATTTTTACATc                                                                                 >  2:1479724/1‑134 (MQ=255)

                                                      ttGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATc                                                                                 <  1:2665110/134‑1 (MQ=255)

                                                       tGTAATTAATCGGCGATATAGTCTGTACTTTACCTAAAATGTGTCTAACCACAATGTTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATTTAGGTCGCCAATTCCATGTTGAATTTGTACATCt                                                                                <  2:472898/134‑1 (MQ=255)

                                                       tGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTCTTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTAGCCAATTCCATGTTGAATTTATACATCt                                                                                >  2:979840/1‑134 (MQ=255)

                                                         tAATTAACCGGCAATATAACCTGCATTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTc                                                                              <  1:1215584/134‑1 (MQ=255)

                                                         tAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTc                                                                              <  1:2064655/134‑1 (MQ=255)

                                                           aTTAACCTGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTccc                                                                            <  1:1016094/134‑1 (MQ=255)

                                                            ttAACCGGCAATATCCTCTGCACTTTAACTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCa                                                                           <  1:2216794/134‑1 (MQ=255)

                                                            ttAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCa                                                                           <  1:1182358/134‑1 (MQ=255)

                                                               aCCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAACTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACtt                                                                        >  1:1709301/1‑134 (MQ=255)

                                                               aCCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGATTTTGGGTAAACAATATGTAGGTCGCCAATACCATGTTCAATTTGTACATCTTCCCACtt                                                                        >  1:1350647/1‑134 (MQ=255)

                                                                 cGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTtat                                                                      >  2:423383/1‑134 (MQ=255)

                                                                  ggtaaTATAATCTTGACTTATCCAAAAACGTTTCTATCAACAATGTTATTATTTTTTTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTTCATGTTGAATTTGTACATCTTCCCACTtata                                                                     <  2:2529313/131‑1 (MQ=255)

                                                                     aaTATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGTTATTATTTTCCTTAATATTTTCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATAc                                                                  <  2:1460951/134‑1 (MQ=255)

                                                                     aaTATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCCGCTTTTGTTACGTCAATATATAAATCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATAc                                                                  >  1:419389/1‑134 (MQ=255)

                                                                      atatGAGCTGTAGTTTATCTAATATGTTTCTAACTAGAATTGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTAAATTTGTACATCTTCCCACTTATATACa                                                                 <  2:2668433/134‑1 (MQ=255)

                                                                      atatAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCTTTTTTATACa                                                                 <  2:631112/134‑1 (MQ=255)

                                                                      atatAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACa                                                                 <  2:639999/134‑1 (MQ=255)

                                                                       tataACCTGCACTTTACCTAAAATGTGTCTACCATCAATTTTAGTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTAAATATATAGATCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACAt                                                                >  1:1244687/1‑134 (MQ=255)

                                                                       tataACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTATGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACAt                                                                >  1:2055630/1‑134 (MQ=255)

                                                                       tataACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCC‑TAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATa                                                               >  2:1932879/1‑134 (MQ=255)

                                                                        ataACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATa                                                               <  2:1987137/134‑1 (MQ=255)

                                                                            ccTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACt                                                           >  2:2054612/1‑134 (MQ=255)

                                                                              tGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTgg                                                         <  2:2199862/134‑1 (MQ=255)

                                                                                 aCTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGgtgt                                                      <  1:1167481/134‑1 (MQ=255)

                                                                                 aCTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCAAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGgtgt                                                      <  1:2574546/134‑1 (MQ=255)

                                                                                 aCTTTACCTAAAATGTGTCTAACAACAATGGAATTATTTTCCTTAATATTTGAAGCTATTGTTAAGTCAATATGTAGGTCGCCAATTCGATGTTGCATGTGTACATCTTCCCACTTATATACATAAACTAgtgt                                                      >  2:180818/1‑134 (MQ=255)

                                                                                  ctgttccTAAAGTGTGTAGAACAACATTTGTCTTAGTTTTCTTAATATTTGTAGCTTTTGTTAAGTCAATGTGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTa                                                     <  2:2679687/129‑1 (MQ=255)

                                                                                  cTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTa                                                     <  2:2239704/134‑1 (MQ=255)

                                                                                    ttACCTAATATGTGTCTAACTACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATCTACATAAACTGGTGTACg                                                   <  2:131818/134‑1 (MQ=255)

                                                                                    ttACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGTTGTACg                                                   >  2:815802/1‑134 (MQ=255)

                                                                                     tACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGt                                                  >  1:541363/1‑134 (MQ=255)

                                                                                     tACCTAAAATGTGACTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTATTTTTAAGTCAATATGTAGGTCGCCAATTCTATGTTGATTTTGTACATTTTTCCACTTATCTACATCAACTATTGTaatt                                                  >  2:2214566/1‑131 (MQ=255)

                                                                                      aCCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGtt                                                 >  1:2796104/1‑134 (MQ=255)

                                                                                        cTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGc                                               <  1:738328/134‑1 (MQ=255)

                                                                                            aaTGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACTTGAACTGGTGTACGTTGCTCAc                                           <  2:1044800/134‑1 (MQ=255)

                                                                                              tgtgTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCACACTTATATACATAAACTGGTGTACGTTGCTCACCa                                         >  2:2596226/1‑134 (MQ=255)

                                                                                               gtgtCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCaa                                        <  1:1973490/134‑1 (MQ=255)

                                                                                                 gtCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCaacc                                      >  2:1073429/1‑132 (MQ=255)

                                                                                                 gtCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTAAATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAAc                                      >  1:1126316/1‑134 (MQ=255)

                                                                                                 gtCTAACAACAATGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAAc                                      >  1:2331515/1‑134 (MQ=255)

                                                                                                 ctgtAACAACAAACTTTTTCTTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAAc                                      <  1:2083592/131‑1 (MQ=255)

                                                                                                    tAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCAc                                   <  2:558577/134‑1 (MQ=255)

                                                                                                     aacaacAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTTAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACt                                  <  1:2236139/134‑1 (MQ=255)

                                                                                                      acaacaATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACtt                                 <  1:1851994/134‑1 (MQ=255)

                                                                                                      acaacaATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACtt                                 >  1:2304122/1‑134 (MQ=255)

                                                                                                       catcaaTGGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTTAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACttt                                <  2:1400873/131‑1 (MQ=255)

                                                                                                       caacaaTTGTATTATTTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACttt                                <  2:1636440/134‑1 (MQ=255)

                                                                                                       caacaaTGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTATGTTGTTAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACttt                                <  1:320870/134‑1 (MQ=255)

                                                                                                          caaTGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGt                             <  2:2758351/134‑1 (MQ=255)

                                                                                                              ggTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTTAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTAACCAAACCCCTTTTGTTtaa                         >  2:7411/1‑134 (MQ=255)

                                                                                                                 attattTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTTTTtaataa                      >  2:915364/1‑134 (MQ=255)

                                                                                                                 attattTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTtaataa                      >  2:2377453/1‑134 (MQ=255)

                                                                                                                  ttattTTCCTTAATATTTGAAGCTTTTGTTATGTCAATATGTAGGTCGCCAATTCCATGTTGATTTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATaaa                     <  1:956623/134‑1 (MQ=255)

                                                                                                                   tattTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTAGGTTGCTCACCAAACTACTTTTATTtaaaatcg                    >  2:2015522/1‑129 (MQ=255)

                                                                                                                    attTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAg                   >  2:519903/1‑134 (MQ=255)

                                                                                                                    attTTCCTTAATATTTGAAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAg                   >  2:2284828/1‑134 (MQ=255)

                                                                                                                     ttttCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTCTTGTTTAATAAAAGa                  >  2:2508637/1‑134 (MQ=255)

                                                                                                                      tttCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGAt                 <  1:1151230/134‑1 (MQ=255)

                                                                                                                         ccTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGt              >  1:1509336/1‑134 (MQ=255)

                                                                                                                         ccTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACACCTTTTGTTTAATAAAAGATGGt              >  2:1873166/1‑134 (MQ=255)

                                                                                                                          cTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTg             >  1:726031/1‑134 (MQ=255)

                                                                                                                               tatTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTc        <  1:2500327/134‑1 (MQ=255)

                                                                                                                               tatGTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCAAGTTGAAATTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTc        <  1:2010826/134‑1 (MQ=255)

                                                                                                                                  ttGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATa     >  2:126960/1‑134 (MQ=255)

                                                                                                                                   tGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATaa    >  2:1192537/1‑134 (MQ=255)

                                                                                                                                    gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAAc   >  1:2409947/1‑134 (MQ=255)

                                                                                                                                     cAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTTCATGTTGAATTTGTACATCTTCACACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAAcc  <  1:991557/134‑1 (MQ=255)

                                                                                                                                     cAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCCCGTATATGCATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAAcc  <  2:255426/134‑1 (MQ=255)

GATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACC  >  USA300TCH1516_ALE/2028058‑2028324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`