BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,858	C→T	6.2%	S776S (TCG→TCA)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,858	0	C	T	6.2%	1009.4 / 3.2	447	S776S (TCG→TCA)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base C (211/171); new base T (22/25); total (249/198)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 457 total aligned reads displayed.

CTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGC  >  USA300TCH1516_ALE/2774725‑2774991

ctgggtcggagtCGTTATCTGAATCGGAGTTGCTCTCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATcc                                                                                                                                       <  2:1797507/134‑1 (MQ=17)

 tgagtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGACTCCCTTTCTA‑AATCCg                                                                                                                                      >  1:2538747/1‑134 (MQ=255)

     tctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGCGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑CTTCCGACTc                                                                                                                                  >  1:643642/1‑134 (MQ=255)

      ctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCg                                                                                                                                 >  1:1380846/1‑134 (MQ=255)

      ctgagtaGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑ATTCCGAAACg                                                                                                                                 >  2:2809900/1‑134 (MQ=14)

        gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAATCTGAGTCGCTGTCTGAATATGAAACAATGTCTG‑AATGAGAGTCGCt                                                                                                                               >  1:464863/1‑134 (MQ=14)

        gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCACTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAGTCACTGTCTG‑AATCAGAGTGGCt                                                                                                                               >  2:2094183/1‑134 (MQ=14)

          gtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTat                                                                                                                             >  1:2282301/1‑134 (MQ=255)

           tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTATGAGTCTGAGTCGCTATCTGAGTATGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCTGAATCGCTatc                                                                                                                            >  1:1304419/1‑134 (MQ=255)

            ctcTATCTGAAGCCGAGTCGCTATCCGGGTCTGAGTCGCTATCTGAGTGTGAGTCGTTGTCTGTGTCTGAGTCGCTATCCGAGTATCCGACCCAGTCGGGTTGTGGGTCCCTGTCTG‑AATCCGAATCGCTatct                                                                                                                           <  2:2664433/132‑1 (MQ=9)

             gatATCTGTATCGGAGTCTCTGTCTGAGTCTGTTGCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCGGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTatctg                                                                                                                          <  2:620025/132‑1 (MQ=14)

              cTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTatctga                                                                                                                         >  1:2067232/1‑134 (MQ=255)

                aTCTGATTCCGGGTCGCTATCCGCTGCTGAGTCGGTATCTGAGTCTGACTCGCTGTCTGGGTGGGAGTCGCAATCCGTGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTatctgaat                                                                                                                       <  2:2174448/134‑1 (MQ=18)

                     aaTCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTAtctgaatctgaa                                                                                                                  >  1:2624247/1‑134 (MQ=255)

                       tCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCtgaatctgaatc                                                                                                                <  1:984489/134‑1 (MQ=255)

                       tCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCtgaatctgaatc                                                                                                                <  1:1644879/134‑1 (MQ=255)

                        ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGGATCTGACTCACTGTCTG‑AATCCGAATCGCCATCtcgatctgaatcc                                                                                                               >  2:1353956/1‑133 (MQ=18)

                        ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCg                                                                                                               <  2:946383/134‑1 (MQ=255)

                           aGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTA‑AATCCGAATCGCTATCGGAGTCTGAATCTCTa                                                                                                            >  2:2537556/1‑134 (MQ=255)

                            gTCGCTATCCGAGCCTGATGCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCTGAATCACTATCTGTGTCTGAATCGCTAt                                                                                                           >  2:2515898/1‑134 (MQ=14)

                             tCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAATCGCTGTCTGAAACTGAATCCCTGTCTG‑AATCCGAAACGATACCCGAAACTCAATCGCTacc                                                                                                          >  2:1536861/1‑132 (MQ=17)

                               gCTATCCGAGTCTGACTAGCTCTCAGAGCCAGAATCGCTATCTGAGACTGACTCGCTATCCGAGTCAGAATCGCTGTCTGAATCTGAATCACATTCTC‑ACTACAAATCGATATCTAAAACCAAATCGCTaccaa                                                                                                        >  2:1606535/1‑130 (MQ=9)

                                   tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGATACGCCATTTGGATGTGGA‑AAGGATCCGTAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTc                                                                                                    >  1:545024/1‑134 (MQ=17)

                                     cGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTg                                                                                                  >  1:1881416/1‑134 (MQ=255)

                                       aGTCTGAGTCGCTATCTGAGTCTGATTTGTGGAAATAGTCGGAGTTGCTATTCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                                <  1:2703931/134‑1 (MQ=17)

                                       aGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTG‑AATCGGAATCGCTATCTGAATCTGAATCGCTGTCCGAGCCTGAg                                                                                                >  2:276636/1‑134 (MQ=14)

                                        gTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCAATCTGAATCTGAATCGCTGTCTT‑AATCGGCATCGCCATCTCATTCTGAATAGCTATCTGATTATGaat                                                                                               >  2:2358291/1‑132 (MQ=14)

                                           tGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGTTTCGCTATCCTAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                            <  1:127056/134‑1 (MQ=255)

                                            gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAAACTGAATGACTGTCTG‑TATACGAATAGCTATCTGAAACTAAAACGCTCTCAGATGCTGacacacc                                                                                           >  2:1986142/1‑128 (MQ=14)

                                                  cTATCTGAGTCTGAATCTCTGTTTGAGTCTTTGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctga                                                                                     <  1:1217818/134‑1 (MQ=255)

                                                       tGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGCATCTGAATCACTGTCTG‑AATCCGAATCGATATATCTCTAAGAAAAGCTATCCGAGTCTGAGTCGCTGtctgaatctg                                                                                >  1:931455/1‑134 (MQ=18)

                                                       tGAGTCGGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTG‑AATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctg                                                                                <  2:1897466/134‑1 (MQ=255)

                                                        gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctga                                                                               >  1:2110087/1‑134 (MQ=255)

                                                        gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctga                                                                               <  2:1493804/134‑1 (MQ=255)

                                                        gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctga                                                                               <  1:109675/134‑1 (MQ=255)

                                                         aGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTG‑AGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                                                              <  2:1246586/134‑1 (MQ=255)

                                                           tCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTG‑AATCTGAATCGCTGTCTGAGTCTGGTTGGACACCCCAGTCTGAATCGCTATCtgactcttaatc                                                                            >  2:1288233/1‑134 (MQ=11)

                                                           tCTGAATCGCTGTCTGAGTCCGAATCGCCATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTctgcatctgaata                                                                            >  2:1762050/1‑133 (MQ=17)

                                                            cTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCTGAGTCGCTGTCTTAATCTGAATCGCTCTCCGAGTCTGAATCGCTGTCtgaatctgaatca                                                                           >  2:329308/1‑133 (MQ=17)

                                                            cTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCg                                                                           >  1:1181638/1‑134 (MQ=255)

                                                              gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                         <  1:2596184/134‑1 (MQ=255)

                                                              gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                         >  1:753410/1‑134 (MQ=255)

                                                              gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGACGCTGTCTGAATCTAAATCGCt                                                                         >  2:2403651/1‑134 (MQ=255)

                                                                aTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTG‑AATCTGAATGGGTATCTGAATCTGAATCGCTATTCGAGTCTTAGTTGGTGTCTGAATCTCAATCGCtat                                                                       >  2:2867941/1‑132 (MQ=14)

                                                                  cGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCTGAATCGCTGTCTGAATCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAATCTGAATCGCTGTCt                                                                     >  2:825496/1‑134 (MQ=14)

                                                                   gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTATCTG‑AATCCAAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTg                                                                    >  1:1595801/1‑134 (MQ=255)

                                                                    ttGTCTGAGTCTGATTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGGCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGa                                                                   <  1:683924/133‑1 (MQ=255)

                                                                      gtttgagtctgattcTCTATCCGAGTCTGAGTCGTTGTCTTATTCTGATTCATTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                 <  1:1012721/131‑1 (MQ=18)

                                                                      gtctgagtctgaGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                 <  2:2632365/134‑1 (MQ=255)

                                                                      ggctgagtctgagtCGCTATCCGGGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                 <  2:764807/132‑1 (MQ=255)

                                                                             tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATc                                                          <  2:1207238/134‑1 (MQ=255)

                                                                              ctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCg                                                         >  1:1448269/1‑134 (MQ=255)

                                                                               tgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGc                                                        <  1:1165798/134‑1 (MQ=255)

                                                                                gagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCACTGTCTGAATATGAATCGCTGTCTGAGTCCGAATCGCt                                                       >  1:488478/1‑134 (MQ=255)

                                                                                gagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCGG‑AAGCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTATGAGTCGCTGTCTGAGACTGAACCGCTGTCTGAGTACGACTCGCt                                                       >  2:2054978/1‑134 (MQ=17)

                                                                                   tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                    >  1:384789/1‑134 (MQ=255)

                                                                                     gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTTAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctg                                                  <  1:340777/134‑1 (MQ=255)

                                                                                      cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga                                                 >  1:2807918/1‑134 (MQ=255)

                                                                                      cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCACTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga                                                 >  1:1827507/1‑134 (MQ=255)

                                                                                        aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTATCTGAATCTGAATCACTATCTGAGTCCTAATCGCTatctgact                                               >  1:221288/1‑132 (MQ=14)

                                                                                         tcggtgTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatc                                              <  2:516845/129‑1 (MQ=255)

                                                                                         tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGCATCCGAATCGCTatctgaatc                                              >  1:1475103/1‑134 (MQ=255)

                                                                                         tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTATGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatc                                              >  2:437237/1‑134 (MQ=255)

                                                                                           cGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTATCTAAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatctg                                            >  1:246048/1‑134 (MQ=255)

                                                                                            gAGTTTGAGTCGCTGTCTGAATCTGAATCGCTGTCTG‑AATCCTAAGCGCTATGTGAAGCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatctga                                           <  2:873691/134‑1 (MQ=17)

                                                                                             aGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                          >  1:1661309/1‑134 (MQ=255)

                                                                                             aGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGACTCGCTATCTGAATCTGAATCGCGGTCTGAGTCAGAATCGCTATCTGAATCTGAg                                          >  1:2741457/1‑134 (MQ=255)

                                                                                              gTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGt                                         >  1:2003562/1‑134 (MQ=255)

                                                                                                cTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCg                                       <  2:1380846/134‑1 (MQ=255)

                                                                                                  gAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCt                                     <  2:545024/134‑1 (MQ=255)

                                                                                                   aGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTAAATCTGAATCGCTGTCTGAGTACGAATCGCTATCTGAATCTGAGTCGCTg                                    >  2:740756/1‑134 (MQ=255)

                                                                                                   aGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTTAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                    <  1:1051424/134‑1 (MQ=255)

                                                                                                     tCGCTGTCTGCATCTGAATCACTGTCTG‑ACTCTGTATCGTTATCTGTATATGAGTCGTTATCCGAGTCTGAGTCGCTGTATGAATCTGAATCGCTGTCTGAGTCAGAATCGCTATCTGAATCTGAGTCGCTgtc                                  <  1:585710/134‑1 (MQ=14)

                                                                                                     tCGCTGTCTGAATCTGAATCACTGTCTG‑AATCTGAATCGCTATCTGAATCTGAAGCGCTATCCGAGTCGGAGTCGCTGTCTGCATCTGAATCGCTGTATGAGTCTGAATCGCAATATGATTCTGCGTCGccttc                                  >  2:1699080/1‑130 (MQ=14)

                                                                                                     tCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtc                                  >  1:2046658/1‑134 (MQ=255)

                                                                                                     tCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtc                                  >  1:2141957/1‑134 (MQ=255)

                                                                                                      cGCTGTCTGAATCTGAATCACTGTCTG‑AATTCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                                 <  1:437560/134‑1 (MQ=255)

                                                                                                      cGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                                 <  1:2721957/134‑1 (MQ=255)

                                                                                                          gTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                             <  1:1691920/134‑1 (MQ=255)

                                                                                                           tctgaatctgaaTCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGACTCGCCgtctgagtc                            >  2:1080493/1‑134 (MQ=255)

                                                                                                           tccgaacctgaatcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                            <  1:1778847/131‑1 (MQ=255)

                                                                                                             tgaatctgagtcGCTGTCTG‑AATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                          <  2:2441036/134‑1 (MQ=255)

                                                                                                             tgaatctgaatcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                          >  1:2397392/1‑134 (MQ=255)

                                                                                                              gaatctgaatcACTGTCTG‑AATCCGAATCGCTATCTGAATCTAAAGCGCTGTCGGAATCTGGTGTGCTCTGTGATTTAGAATCGCTTTGTGAGTCTGAATTGCTATCTCAATCTGAGTCGCGgttcgattctga                         >  2:1630240/1‑134 (MQ=2)

                                                                                                                 tctgaatcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                      >  1:2580307/1‑134 (MQ=255)

                                                                                                                 tctgaatcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTATCTGAATCTGAATCGCTATCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCtgaatctgaatc                      >  1:859188/1‑134 (MQ=255)

                                                                                                                    gaatcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTCAGTCCGAATCGCTATCTGAATATAAGTCGCTCTCCGCATCTGAATCGCt                   >  2:420371/1‑134 (MQ=17)

                                                                                                                       tcACTGTCTG‑AATCCGAATCGCTGTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTGTGAATGTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                >  2:1034064/1‑134 (MQ=255)

                                                                                                                       tcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGTTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                <  1:523476/134‑1 (MQ=255)

                                                                                                                       tcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTatc                >  2:363910/1‑134 (MQ=255)

                                                                                                                       tcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                >  1:1563157/1‑134 (MQ=255)

                                                                                                                       tcACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                >  1:610792/1‑134 (MQ=255)

                                                                                                                         aCTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg              <  1:2503708/134‑1 (MQ=255)

                                                                                                                          cTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATTCGTGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga             <  1:1005241/134‑1 (MQ=255)

                                                                                                                            gTCTT‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat           <  1:2335542/134‑1 (MQ=255)

                                                                                                                            gTCTG‑TATCCGAATCGCTATCTTAATCCGAATCGCTTTCCGAGTCTGAGTCGCTGTCTGATTCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat           <  2:1097221/134‑1 (MQ=14)

                                                                                                                            gTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTTGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat           <  1:801537/134‑1 (MQ=255)

                                                                                                                             tCTG‑AGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGCATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc          >  1:1319703/1‑134 (MQ=255)

                                                                                                                                  aaTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAACCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg      <  1:1532029/134‑1 (MQ=255)

                                                                                                                                   aTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATTGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATAGCTAGATGAATCTGAgc     >  2:2286280/1‑133 (MQ=14)

CTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTG‑AATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGC  >  USA300TCH1516_ALE/2774725‑2774991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`