BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,651	C→T	6.5%	D170D (GAC→GAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,651	0	C	T	6.5%	561.0 / 16.7	218	D170D (GAC→GAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (73/125); new base T (5/13); total (78/140)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 218 total aligned reads displayed.

CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTT  >  USA300TCH1516_ALE/110521‑110782

 ggTTAAAATAGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAATTCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                 <  1:2343168/134‑1 (MQ=255)

           gTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAATAATAAAAATAGATATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                       <  1:1380617/134‑1 (MQ=255)

              gATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGTCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                    <  2:1084274/134‑1 (MQ=255)

                    aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAACCTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                              >  1:45065/1‑134 (MQ=255)

                    aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                              >  2:1756687/1‑134 (MQ=255)

                      aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                            <  1:1797853/134‑1 (MQ=255)

                       aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagaact                                                                                                           >  2:1502025/1‑131 (MQ=255)

                       aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGt                                                                                                           <  2:1151224/134‑1 (MQ=255)

                       aTCATACCAAAAAAAAAAATTAAATACTAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGt                                                                                                           <  2:289907/134‑1 (MQ=17)

                          aTTTCAACAAAAGAAATTAAAGATTAAATAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                        <  2:95528/134‑1 (MQ=255)

                            tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAACCTTTAAGT‑TCTTTTTTAAATATGGAGAATTTAAAAATTAGAAAAATAATAAAGACGGAGATATTTAATATAATACAGAGGTGCCGa                                                                                                      >  1:1049646/1‑134 (MQ=17)

                            tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAAT‑TCTATATACAATATAGCAACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                      >  1:54644/1‑134 (MQ=17)

                             ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                     <  2:2085427/134‑1 (MQ=255)

                             caaagtaaaGAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAATATGGATATATTTCATATAATCCAGAGGTGCCGAg                                                                                                     <  2:2006477/128‑1 (MQ=17)

                             caaaaaaaaGATATTGAATTTTAATAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                     <  2:1721976/129‑1 (MQ=17)

                              caacaaAAGAAATTAAAGATTAAAAAATAAAAAAACAAAT‑AAAGGCTTTAAATGTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                    <  1:1232469/134‑1 (MQ=17)

                              caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGaat                                                                                                    <  2:2830036/134‑3 (MQ=255)

                               aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACATTAAGT‑TCTTTATTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACTGAGATATTTCATATAATCCAGAGATGCAGAttt                                                                                                   >  2:2509666/1‑134 (MQ=17)

                                  aaaaGAAATTAAAGATGAAAAAATAAAAAAAGAATTCTAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACCTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAtt                                                                                                <  1:413721/134‑1 (MQ=255)

                                      gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATAGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAATACAGAGATGCCGAGTTATTCGGc                                                                                            >  2:677368/1‑134 (MQ=255)

                                        aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGTCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                          <  1:1535248/134‑1 (MQ=255)

                                              aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATATCATATAATACAAAAATACCAAGTTATTCGGCTAAATATc                                                                                    >  1:298199/1‑134 (MQ=18)

                                               gaagAAAAAATAAAAAAAGAAAGCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                   <  2:2859833/131‑1 (MQ=255)

                                                   aaaaaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAACTATAAAGTCGGCAGGAAAGCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                               <  2:2046783/134‑1 (MQ=17)

                                                   aaaaaaTAAAAAAAGAAATAGGAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                               <  2:1930079/134‑1 (MQ=255)

                                                     gaaaTAAAGAAAGGAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGTGACTTTAAAAATTTGAAAAATTATAAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                             <  1:588488/133‑1 (MQ=255)

                                                     aaaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                             <  2:1561021/134‑1 (MQ=255)

                                                      gaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAAGATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                            <  2:1782409/133‑1 (MQ=255)

                                                      aaaTAAAAAAAGAAATCTAAAACTTTAAGT‑TCTTTGTTCAATATGGTGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                            <  1:259375/134‑1 (MQ=255)

                                                      aaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAATAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                            <  2:1018706/134‑1 (MQ=255)

                                                      aaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                            <  1:1548575/134‑1 (MQ=255)

                                                      aaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                            >  1:2077241/1‑134 (MQ=255)

                                                      aaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATACAGAGGTGTCGAGTGATTCGGCTAAATATCAATTAACt                                                                            <  1:2730481/134‑1 (MQ=255)

                                                      aaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATACAGAGGTGCTGAGTTATTAGGCTAAATATGAATTatgc                                                                            >  2:1706853/1‑131 (MQ=17)

                                                      aaaTAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTACCGAGTTATTCGGCTAATTATCAATTAACt                                                                            >  2:2542284/1‑134 (MQ=255)

                                                      aaaGAAAAAAAGAAATCGAAAACTTTAAGT‑TCTGTTTGCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                            <  2:1339253/134‑1 (MQ=17)

                                                         tagaaaaaTAAATCTAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                         <  2:1220053/131‑1 (MQ=255)

                                                         tAAAAAAAGAAATCGAAAACATTAAGT‑TCTTTGTTCAACCTGGCGTCTATAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                         <  2:2784249/134‑1 (MQ=17)

                                                            aaaaaGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAATAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                      <  2:2089354/134‑1 (MQ=255)

                                                              aaaGAAATTAAAATATTTAATT‑TTATTGATAAATATTGCGAATTTAAAAATTTGAAAAATTATAAAGATGGAGATATTTCATATAATCTAGAGGTGCCTAGTTAGTCGGCTAAATATCAATTAACTAAtgatga                                                                    <  2:1877370/134‑1 (MQ=17)

                                                                  aaaTCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                <  1:636958/134‑1 (MQ=255)

                                                                   aaTCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                               <  1:1589/134‑1 (MQ=255)

                                                                   aaTCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                               <  1:2237314/134‑1 (MQ=255)

                                                                     tCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                             <  2:1658271/134‑1 (MQ=255)

                                                                      cGATAAGTTTAAGC‑TTGTTGTTTAATATGGCCACTTTAAAAATTTGAAAGATGATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                            <  2:2432573/134‑1 (MQ=17)

                                                                      cGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                            >  1:2611750/1‑134 (MQ=255)

                                                                        aaaaCATTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTTAAAAATTATAAAGACGGAGATATTTCATATAATCCATAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                          <  1:953843/134‑1 (MQ=255)

                                                                         aaaCTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGATATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                         <  1:2413685/134‑1 (MQ=255)

                                                                         aaaCTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGATATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                         <  1:2173331/134‑1 (MQ=255)

                                                                         aaaCTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                         <  2:1382072/134‑1 (MQ=255)

                                                                         aaaCTTTAAGT‑TATTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                         <  2:2145328/134‑1 (MQ=255)

                                                                          aaCTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                        >  1:2620787/1‑134 (MQ=255)

                                                                          aaCTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTAACTAATGATGATTATAATGTaaa                                                        >  1:2434245/1‑134 (MQ=255)

                                                                                aaGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                  >  1:707787/1‑134 (MQ=255)

                                                                                  gT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACTGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                <  1:1909226/134‑1 (MQ=255)

                                                                                  gT‑TCGTTTTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                <  1:1513088/134‑1 (MQ=255)

                                                                                   t‑tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                               >  2:1207351/1‑134 (MQ=255)

                                                                                      cTTTGTTCAATATGGCGACTTTAAAAATTTGAACAATTATAAAGACGGAGATATTTCATATAATAAAGAGGTACCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                             >  1:722311/1‑134 (MQ=255)

                                                                                      cTTTGTTCAATATGGCGACTTTAAAAATTCGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                             <  1:1673688/134‑1 (MQ=255)

                                                                                       tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                            >  1:203471/1‑134 (MQ=255)

                                                                                       tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGATATTCGGCTAAATATCAATTAACTAATCATGATTATAATGTAAAACAAATACGCaa                                            >  2:81713/1‑134 (MQ=255)

                                                                                       tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATTCAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                            >  1:937603/1‑134 (MQ=255)

                                                                                         tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTAGAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                          <  2:465702/134‑1 (MQ=255)

                                                                                          gTTCAATATGGCGACATTAAAAATTAGAAAAATTATAAAGACGGAGATATTTCATATAATCCAAAGTTTCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                         >  2:879917/1‑134 (MQ=17)

                                                                                            tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTTTTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                       <  2:1842744/134‑1 (MQ=255)

                                                                                            tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATAGAATCCAGAGGTGCTGAGCTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                       <  1:1649244/134‑1 (MQ=255)

                                                                                             cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                      >  1:1019734/1‑134 (MQ=255)

                                                                                               atatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCTAGAGGTGCCGAGTTCTTGGGGGGAAAAGCAATTAAATAATGATGAATATAATGTAAAACAATTACGAAAAAgatagg                                    >  1:2847466/1‑132 (MQ=18)

                                                                                                  tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATTCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                 <  1:497581/134‑1 (MQ=255)

                                                                                                  tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                 <  2:314632/134‑1 (MQ=255)

                                                                                                  tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                 <  2:374596/134‑1 (MQ=255)

                                                                                                    gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                               >  1:2696154/1‑134 (MQ=255)

                                                                                                    gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGACCAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                               >  1:1357183/1‑134 (MQ=255)

                                                                                                    gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                               >  1:886238/1‑134 (MQ=255)

                                                                                                     ctaCTTTAAAAATTTGAAAAATTATAAAGACCGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATACTGTAAGTCAAAGCTTCAAAAgatatgatattc                              <  2:632259/132‑3 (MQ=17)

                                                                                                       aCTTTAAAAATTTGAAAAATTATAAATACGGAGATATTTCATATTATCCAGAGTTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcg                            <  1:2470486/134‑1 (MQ=255)

                                                                                                          ttaaaaattttaaaaattATAAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                         <  1:2050959/134‑1 (MQ=255)

                                                                                                           tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                        <  1:853725/134‑1 (MQ=255)

                                                                                                              aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                     >  2:341507/1‑134 (MQ=255)

                                                                                                              aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                     >  2:1676484/1‑134 (MQ=255)

                                                                                                               aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAGAACAATTACGCAAAAGATATGATATACCTACGAGTaa                    >  2:930029/1‑134 (MQ=255)

                                                                                                               aaTTTGAAAAATTATAAAGACGGAGATATGTCATATAATCGAGAGGTGCCGAGATATTCGGCTAAATATCAATTAAATAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATCTCCGAACGAGtca                    >  2:2348577/1‑132 (MQ=17)

                                                                                                                  ttGAAAAATTATAAAGACGGAGATATTTCATATAATGCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                 <  2:523114/134‑1 (MQ=255)

                                                                                                                  ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                 <  1:1113060/134‑1 (MQ=255)

                                                                                                                  ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATAATATACAGACGAGTaaacc                 >  2:2847659/1‑132 (MQ=255)

                                                                                                                   taatgaaTTAGAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                <  2:221307/129‑1 (MQ=14)

                                                                                                                     attaaTTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc              <  2:859573/131‑1 (MQ=255)

                                                                                                                     aaaaaTTATAAAGACGGATATATTTCATATAATTCAGAGGTGCCGAATTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc              <  1:1997574/134‑1 (MQ=255)

                                                                                                                     aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGCGTAAAGCTcc              >  2:564392/1‑134 (MQ=255)

                                                                                                                     aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc              >  2:1114422/1‑134 (MQ=255)

                                                                                                                     aaaaaTGATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc              <  2:653461/134‑1 (MQ=255)

                                                                                                                      tgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa             <  1:2146125/132‑1 (MQ=255)

                                                                                                                        aaTTATAAAGATAGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGGAAAGCTCCaaa           >  2:424916/1‑134 (MQ=14)

                                                                                                                           tataAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt        <  1:1500402/134‑1 (MQ=255)

                                                                                                                             taAAGATGGAGATATATCATATAATCTAGAGGTGCCGAGATATTCAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt      <  2:889726/134‑1 (MQ=14)

                                                                                                                             taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt      <  2:7259/134‑1 (MQ=255)

                                                                                                                             taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGATAAATATCAATTCACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt      >  2:2498081/1‑134 (MQ=17)

                                                                                                                              aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt     <  1:2221882/134‑1 (MQ=255)

                                                                                                                                aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt   <  1:985836/134‑1 (MQ=255)

                                                                                                                                 gACGGAGATATTTCATATAATCCAGAGCTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt  <  1:1572948/134‑1 (MQ=255)

CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGT‑TCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTT  >  USA300TCH1516_ALE/110521‑110782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`