| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 96.8% | 94.2 / ‑4.6 | 31 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (9/21); total (9/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA > USA300TCH1516_ALE/2532160‑2532433 | aaatgtATTTGAAATCAGTTTTTAATAAAATAAAAAAATAGGATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTtactac < 2:66542/138‑1 (MQ=255) aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑a < 1:23252/141‑1 (MQ=255) ttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTGATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑Att > 1:180650/1‑141 (MQ=255) aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTc > 1:278307/1‑141 (MQ=255) tttatGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTa < 2:228332/141‑1 (MQ=255) ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTacac < 1:218072/141‑1 (MQ=255) ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGGTCAATTCAGATAGAGATAAGATATTATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTacac < 1:191010/141‑1 (MQ=255) aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATc > 2:2280/1‑141 (MQ=255) taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGTGATGGGATAAAATGACATCGGCGCTGGTGACGTGGGGTGAAAGCTCCGCTGTTGCTACAAAGC‑ATTTTTCACATTACACACAATCAGc < 1:143995/141‑1 (MQ=255) aaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATATAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGc < 2:183666/140‑1 (MQ=255) aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTAtt > 2:118240/1‑141 (MQ=255) aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTg < 1:72077/141‑1 (MQ=255) ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTAGGATGAGGTCAATGCGTCTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg > 2:50910/1‑141 (MQ=255) ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg < 1:209527/141‑1 (MQ=255) aaaatCAGAGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGGGTTAAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgtg < 2:245810/137‑1 (MQ=255) aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCa < 2:157096/141‑1 (MQ=255) tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc < 1:42930/141‑1 (MQ=255) cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt < 1:186691/141‑1 (MQ=255) tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt < 1:261518/141‑1 (MQ=255) atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg < 1:118240/141‑1 (MQ=255) atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg < 1:123116/141‑1 (MQ=255) gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa < 2:235913/141‑1 (MQ=255) gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa < 1:173991/141‑1 (MQ=255) gAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTTCATCTTGTAGGGGTTAAAGATGACaa > 2:144422/1‑141 (MQ=255) cTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTA‑ATTGTAGCTGTTGTTCATATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATgg < 2:214325/141‑1 (MQ=255) aCGTTGACTTCGGATGAGGTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg < 2:180650/141‑1 (MQ=255) tcaCTTCGGAGCAGTTGAATGCGTTTAGTACAAATC‑ATTTTTTAAAGTACACACAATCAGCTATGCATTACAATCATAGAGGTGATTTAAAAGGGTATGTGCATCTGGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg < 2:300613/139‑1 (MQ=255) gAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa < 1:74989/141‑1 (MQ=255) gAGTTCAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa < 1:2280/141‑1 (MQ=255) cAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGaa > 1:205737/1‑140 (MQ=255) cAATGCGTTTACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc > 1:185690/1‑141 (MQ=255) tttACTACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca > 1:239656/1‑141 (MQ=255) | ATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGC‑ATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA > USA300TCH1516_ALE/2532160‑2532433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |