Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	76.3 / NA	26	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/13);  total (13/13)

ATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAT  >  USA300TCH1516_ALE/1646056‑1646320                                                                                                                                       |                                                                                                                                    aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAAATCTAGCCATCTTG‑GTTCTAATATTGTCTTTAATTCTTTTTCAGTCATACCTCCTTTTac                                                                                                                              >  2:182421/1‑141 (MQ=255)       tGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACACTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                        <  2:18091/141‑1 (MQ=255)                     gTAATGCGCCTCTATGACTAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                          <  2:107361/141‑1 (MQ=255)                                ttATGACCAGATGCTACAATCGTATCTAATGATAGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                               <  2:150466/140‑1 (MQ=255)                                ctATGACCAGACGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                               <  1:71380/141‑1 (MQ=255)                                          aTGCTACAATTGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                                                                     >  1:27266/1‑141 (MQ=255)                                          aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATGCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCCACAatataat                                                                                     >  2:215389/1‑138 (MQ=255)                                               aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTAAGTCATTGCACCTGGTGCAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                >  1:142213/1‑141 (MQ=255)                                                  aTCGGATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                             <  2:63080/141‑1 (MQ=255)                                                       aTCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                        >  1:274394/1‑141 (MQ=255)                                                                       tCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCATTTTCGTCa                                                        >  1:152401/1‑141 (MQ=255)                                                                         tGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCCTTTTCAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc                                                      <  2:35432/141‑1 (MQ=255)                                                                           cTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                    >  2:84490/1‑141 (MQ=255)                                                                                    cTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                           >  1:147846/1‑141 (MQ=255)                                                                                      aG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTAAAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                         <  2:34823/141‑1 (MQ=255)                                                                                       g‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                        >  2:240291/1‑141 (MQ=255)                                                                                           ttttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                     <  2:113379/141‑1 (MQ=255)                                                                                                 ttttTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                               <  2:144658/141‑1 (MQ=255)                                                                                                          tttccTTTAATTCTTTTTCAGTCATGTCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAt                      <  2:157425/138‑1 (MQ=255)                                                                                                          ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTAGTCAGCATCTTTGACTTCTCTAATTTTATCTACAGTAt                      >  2:206822/1‑141 (MQ=255)                                                                                                             ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                   >  2:33007/1‑141 (MQ=255)                                                                                                                 ttaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt               <  2:300502/139‑1 (MQ=255)                                                                                                                      cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACACTATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGCGTTCTCTAACTTTACCTAAAGTATCAGAAAttgttt          >  2:198730/1‑136 (MQ=255)                                                                                                                       tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat         <  1:61025/141‑1 (MQ=255)                                                                                                                         tttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta       <  2:27266/141‑1 (MQ=255)                                                                                                                              gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGATGCATTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACTTCTCTAATTTTATCTACAGTATTAGAACTGCTTATTAATGat  >  2:17567/1‑141 (MQ=255)                                                                                                                                       |                                                                                                                                    ATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAG‑CATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAT  >  USA300TCH1516_ALE/1646056‑1646320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑