Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,416,4580AT100.0% 84.5 / NA 26I216N (ATT→AAT) oppD_4Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/14);  total (12/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGG  >  USA300TCH1516_ALE/1416330‑1416591
                                                                                                                                |                                                                                                                                     
aaTCATAGTAATCCCTCTTTAATTGTGTCCTATATTTAATTAGGCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                           <  1:15623/141‑1 (MQ=255)
       gTAATCCCTCTTTAATTGTGTTCTATATTTAATTTTTGTTGTGGTGTACTGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCaa                                                                                                                    <  1:92488/141‑1 (MQ=255)
           tCCCTCTTTAATTGAGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACa                                                                                                                >  1:137848/1‑141 (MQ=255)
             ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAACGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                              <  2:311322/141‑1 (MQ=255)
                 tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                          <  1:356102/141‑1 (MQ=255)
                     aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                      <  1:155012/141‑1 (MQ=255)
                     aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATCCCTCCGTCCACTGCTTTGAGTAACTCTATAAAGATAATTGTACTGCCAATAATTTAATACATGACTTAAGt                                                                                                      <  2:485099/141‑1 (MQ=255)
                        gtgtTGGATATTTAATTAGAGGTGGATTATACGGATGCAAATGCTCATACATGAAATGAGTAAGATTACCTCGTTCAATGAGTTTACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAt                                                                                                   <  2:274460/141‑1 (MQ=255)
                               atatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGat                                                                                            <  1:209623/141‑1 (MQ=255)
                                         aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATCTGTGATAATAaataat                                                                                  >  2:401007/1‑141 (MQ=255)
                                              ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                             <  1:225665/141‑1 (MQ=255)
                                              gtgaGTAGACGGATGCAAAGGCTGGTATTGGAAATGAGTAATAGTACGTCTTTGAATGATTTGACCGTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTGAAGTTATGGGTGATAATAAATAATGTTTg                                                                             <  2:375916/138‑1 (MQ=255)
                                                cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                           <  2:57114/141‑1 (MQ=255)
                                                    ataCTGATGCTTGTGCTTGTACTTGAAATGATTTGTTGTAGCTCATTCATTGACGTTACCTTCTGTTAAAACATAATTGTTCTGACAATATTTCAATACATGACTTCAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                       <  2:374323/141‑1 (MQ=255)
                                                        ggATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCt                                                                   <  1:464004/141‑1 (MQ=255)
                                                               aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGGCAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATATTTGACCATGTTCTAATACaa                                                            >  2:75142/1‑141 (MQ=255)
                                                                         tttGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaa                                                  <  2:449952/140‑1 (MQ=255)
                                                                          ttGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaat                                                 >  1:10278/1‑141 (MQ=255)
                                                                                           aCCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGAtt                                >  1:428233/1‑141 (MQ=255)
                                                                                                     aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                      >  1:270798/1‑141 (MQ=255)
                                                                                                      tGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATTAATAATGTTTGACCATGTTCTAATACAATATGCTATAATAAATCCATCACTTAATTACCTTTCAAAg                     >  2:439286/1‑141 (MQ=255)
                                                                                                         tttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGGGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAATACCATTACTTGCTTACAGTTAAACGCAt                  >  2:29429/1‑141 (MQ=255)
                                                                                                              ccTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAat             >  2:272686/1‑141 (MQ=255)
                                                                                                                     ttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCaa      >  1:227261/1‑141 (MQ=255)
                                                                                                                        aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATACATCACTTGATTACCATTCAAAGCATCAAATCAAGCAACTg   >  2:315936/1‑141 (MQ=255)
                                                                                                                         aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACtcg  >  1:449667/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                     
AATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGG  >  USA300TCH1516_ALE/1416330‑1416591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: