Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5120AG38.5% 17.3 / 16.5 28S194S (TCA→TCGUSA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (7/9);  new base G (2/8);  total (10/18)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCA  >  USA300TCH1516_ALE/111386‑111635
                                                                                                                              |                                                                                                                           
tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGc                                                                                                                           <  2:61108/129‑1 (MQ=255)
     aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                      <  2:215005/129‑1 (MQ=255)
     aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                      <  1:99119/129‑1 (MQ=255)
       gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                    <  1:122142/129‑1 (MQ=255)
          aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCACAATATAGCAATTTTATAGGTATAATTTATTATAAAAATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                                                 >  1:77165/1‑129 (MQ=17)
                 aaaaaGAAATCGAAAACTTTAAGTTATTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCACAGGTGCCGAGTTATTCAGCTCACTATCAATTAACTa                                                                                                          >  2:99592/1‑129 (MQ=255)
                 aaaaaGAAATCGAAAACTTGAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                          <  2:217128/129‑1 (MQ=255)
                        aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTACtgatga                                                                                                   >  2:182416/1‑129 (MQ=255)
                            gAAAACTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                               <  2:11072/129‑1 (MQ=255)
                            gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                               <  1:44334/129‑1 (MQ=255)
                                aCTTTAAGTTTTTTTTTCAATATAGTAATTTTAAAGATTTAATGAATTACAAATACAAAGACATTTCATATAATCCAGAGGTGCCGAGTTATTCGGTTAAATATCAATTAACTAATGATGATTATAATg                                                                                           <  2:177111/129‑1 (MQ=11)
                                aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATAGATCATATAATTCAGAGGTGTCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                                           <  1:134594/129‑1 (MQ=18)
                                    tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAAAGGTGACGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                       <  1:76947/129‑1 (MQ=17)
                                       gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                    <  1:136057/129‑1 (MQ=17)
                                         tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                  <  1:129156/129‑1 (MQ=255)
                                           tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATAAAAACCAGAGGTGCCGAGTTATTATAGTGAGGAGCAAATTACTAATGCGGATAATAATGTAAAACAATTa                                                                                >  1:232856/1‑129 (MQ=18)
                                           tttGCGCAATAGAGCAATTTTAAATATTTAATGATTTATAAAGATGGTTATATATTATATAATCCAGAGGTGACGAGTTATTCTGCTAAATATCGATTAACAAATGATGATTATAATGTAAAACAATTa                                                                                <  1:224116/129‑1 (MQ=17)
                                            ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                               >  2:113395/1‑129 (MQ=255)
                                             tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                              <  1:161783/129‑1 (MQ=255)
                                                gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATAACGTaa                                                                           >  2:95504/1‑129 (MQ=255)
                                                gcAATATAGAAATTTTAAAGATTTAATGAATTAAAAAAATGTAGATATATCGTATAATCTAAAGGTGATGAGTTATTCGACTAAATATAAATTAACTAAAGAGGATTATAATGTAAAAAAATTACgcat                                                                           >  2:171659/1‑126 (MQ=255)
                                                              ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCATAGATAACGAGTTATACAACTAAATATCAATTAACTAATGATGATTATAATATAAAACAATTACGCAAAAGATATGATATAc                                                             >  1:54495/1‑129 (MQ=14)
                                                                      ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAt                                                     <  2:54485/129‑1 (MQ=255)
                                                                                    aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                       <  1:160351/129‑1 (MQ=255)
                                                                                       tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                    >  1:69974/1‑129 (MQ=255)
                                                                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAATATATGATATACCATTATATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                  <  1:132466/129‑1 (MQ=17)
                                                                                                                  gacGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg         <  2:232856/127‑1 (MQ=255)
                                                                                                                         tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca  >  1:84330/1‑129 (MQ=255)
                                                                                                                              |                                                                                                                           
TAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCA  >  USA300TCH1516_ALE/111386‑111635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: