Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5160CA46.2% 0.3 / 27.1 27Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (6/8);  new base A (3/9);  total (10/17)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCA  >  USA300TCH1516_ALE/111391‑111635
                                                                                                                             |                                                                                                                       
aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                      <  2:215005/129‑1 (MQ=255)
aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                      <  1:99119/129‑1 (MQ=255)
  gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                    <  1:122142/129‑1 (MQ=255)
     aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCACAATATAGCAATTTTATAGGTATAATTTATTATAAAAATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                                                 >  1:77165/1‑129 (MQ=17)
            aaaaaGAAATCGAAAACTTTAAGTTATTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCACAGGTGCCGAGTTATTCAGCTCACTATCAATTAACTa                                                                                                          >  2:99592/1‑129 (MQ=255)
            aaaaaGAAATCGAAAACTTGAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                          <  2:217128/129‑1 (MQ=255)
                   aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTACtgatga                                                                                                   >  2:182416/1‑129 (MQ=255)
                       gAAAACTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                               <  2:11072/129‑1 (MQ=255)
                       gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                               <  1:44334/129‑1 (MQ=255)
                           aCTTTAAGTTTTTTTTTCAATATAGTAATTTTAAAGATTTAATGAATTACAAATACAAAGACATTTCATATAATCCAGAGGTGCCGAGTTATTCGGTTAAATATCAATTAACTAATGATGATTATAATg                                                                                           <  2:177111/129‑1 (MQ=11)
                           aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATAGATCATATAATTCAGAGGTGTCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                                           <  1:134594/129‑1 (MQ=18)
                               tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAAAGGTGACGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                       <  1:76947/129‑1 (MQ=17)
                                  gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                    <  1:136057/129‑1 (MQ=17)
                                    tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                  <  1:129156/129‑1 (MQ=255)
                                      tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATAAAAACCAGAGGTGCCGAGTTATTATAGTGAGGAGCAAATTACTAATGCGGATAATAATGTAAAACAATTa                                                                                >  1:232856/1‑129 (MQ=18)
                                      tttGCGCAATAGAGCAATTTTAAATATTTAATGATTTATAAAGATGGTTATATATTATATAATCCAGAGGTGACGAGTTATTCTGCTAAATATCGATTAACAAATGATGATTATAATGTAAAACAATTa                                                                                <  1:224116/129‑1 (MQ=17)
                                       ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                               >  2:113395/1‑129 (MQ=255)
                                        tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                              <  1:161783/129‑1 (MQ=255)
                                           gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATAACGTaa                                                                           >  2:95504/1‑129 (MQ=255)
                                           gcAATATAGAAATTTTAAAGATTTAATGAATTAAAAAAATGTAGATATATCGTATAATCTAAAGGTGATGAGTTATTCGACTAAATATAAATTAACTAAAGAGGATTATAATGTAAAAAAATTACgcat                                                                           >  2:171659/1‑126 (MQ=255)
                                                         ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCATAGATAACGAGTTATACAACTAAATATCAATTAACTAATGATGATTATAATATAAAACAATTACGCAAAAGATATGATATAc                                                             >  1:54495/1‑129 (MQ=14)
                                                                 ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAt                                                     <  2:54485/129‑1 (MQ=255)
                                                                               aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                       <  1:160351/129‑1 (MQ=255)
                                                                                  tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                    >  1:69974/1‑129 (MQ=255)
                                                                                                    tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAATATATGATATACCATTATATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                  <  1:132466/129‑1 (MQ=17)
                                                                                                             gacGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg         <  2:232856/127‑1 (MQ=255)
                                                                                                                    tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca  >  1:84330/1‑129 (MQ=255)
                                                                                                                             |                                                                                                                       
AAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCA  >  USA300TCH1516_ALE/111391‑111635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: