Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.96.0% 322.6 / 1.1 75coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base . (47/25);  minor base G (0/2);  total (47/28)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAATAAAT  >  CP000731/25524‑25803
                                                                                                                                           |                                                                                                                                            
ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaa                                                                                                                                             <  1:16627/141‑1 (MQ=255)
  gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaa                                                                                                                                           >  2:292208/1‑141 (MQ=255)
       ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                      >  1:275805/1‑138 (MQ=255)
        tATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatata                                                                                                                                     >  1:59814/1‑137 (MQ=255)
        tATTTACACTAGTGCATAAATTTACAGGTATGACAACAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTGATTAGTATACTAAAAA‑AAAAAAAtata                                                                                                                                    <  2:272043/141‑1 (MQ=255)
           ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACAt                                                                                                                                 >  1:44604/1‑141 (MQ=255)
             acacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCt                                                                                                                               >  1:246862/1‑141 (MQ=255)
              cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTa                                                                                                                              >  1:270783/1‑141 (MQ=255)
              aacTAGTGCTTAAATTTACAGGTTTGTCACGAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTa                                                                                                                              <  2:147094/140‑1 (MQ=255)
                cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAACATATACATCTaaa                                                                                                                            >  1:120129/1‑141 (MQ=255)
                cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATAAATCTaaa                                                                                                                            >  1:120182/1‑141 (MQ=255)
                  aGTGTTTAATTTTACAGTTTTGTCAACAATGATTTAAGTATCTTGTTTTTCTTAATATTAAGAAATTTTCTTTATAATTGTATCACTATTTTTAATGATATTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAgg                                                                                                                          <  2:204563/141‑1 (MQ=255)
                  aGTGCTTAAATTTACTAGTTTGTCACGAAGTATTGAAGGAACTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGAAGCACTATTGCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAgg                                                                                                                          <  2:305164/141‑1 (MQ=255)
                  aGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAgg                                                                                                                          <  1:208460/141‑1 (MQ=255)
                      cTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTaa                                                                                                                      >  1:291795/1‑141 (MQ=255)
                      cTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAAAATCCAAAAATTTTCTTTATAAGTGTAGCACTATTACTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAACGTTaa                                                                                                                      >  2:274393/1‑141 (MQ=255)
                        tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTA‑TTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAACGTTTAAAtt                                                                                                                   >  2:244317/1‑141 (MQ=255)
                        tAAATTTACAGGTTTGTCACCAAGGATGGGAATATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAAt                                                                                                                    >  2:81193/1‑141 (MQ=255)
                           aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTaa                                                                                                                 >  2:93442/1‑141 (MQ=255)
                            tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTact                                                                                                                >  2:24024/1‑139 (MQ=255)
                             ttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATg                                                                                                               >  1:54075/1‑141 (MQ=255)
                              tACAGATTTGTCACGAAGTAGTGAAGGATCTTGGTATTGATAATATCAAGAAATTTTCGTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATAATACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                              <  2:233171/141‑1 (MQ=255)
                               aCAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaa                                                                                                             >  1:309205/1‑141 (MQ=255)
                               aCAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTCTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATaaa                                                                                                             >  2:294924/1‑141 (MQ=255)
                                cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                            >  2:157677/1‑141 (MQ=255)
                                cAGGGTTGTCACCAAGTATTGAATGATCTTGGTTTTCTGAATATCCAGAAATTTTCTTTATAATTGTATAACTATTTCTTATGCTGTTTAATTAATATAATACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                            <  2:42641/141‑1 (MQ=255)
                                  ggTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGaaaaa                                                                                                          <  2:272470/141‑1 (MQ=255)
                                   gTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAACTATCTATACAAAGGTTAAATTAATGAAAAAt                                                                                                         <  1:295753/141‑1 (MQ=255)
                                           ccAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAATGTTAAATTAATGAAAAATAGAATTag                                                                                                 >  1:63081/1‑141 (MQ=255)
                                            cAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTaga                                                                                                >  1:196112/1‑141 (MQ=255)
                                              agaaTTGAATGATCTTAGTTTTCTCAATATCCAGAAATTTACTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATATAATTAgaga                                                                                              <  1:69666/138‑1 (MQ=255)
                                               gTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGaa                                                                                             <  2:262498/141‑1 (MQ=255)
                                                 aTTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAata                                                                                           >  1:40752/1‑141 (MQ=255)
                                                   tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAAAAtata                                                                                         >  2:169164/1‑141 (MQ=255)
                                                             tGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                               <  1:181415/141‑1 (MQ=255)
                                                                 tttCTCAATATCCAGAAATTTTCTTTATAATTGTATCACTATTTCTTATGCTGTTTAATTACTATACTATAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGATAAATAGAATCAATGATTCTAGACAACACAAAgaa                                                                           >  2:1270/1‑139 (MQ=255)
                                                                 tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                           >  1:284993/1‑141 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtctaa                                                                   >  1:301889/1‑139 (MQ=255)
                                                                                  aTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAAAGAATATAGAAAACAAAAAGGAATTTTTAAAGAAACttt                                                          >  2:294037/1‑141 (MQ=255)
                                                                                    tttCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTaa                                                        >  1:168601/1‑141 (MQ=255)
                                                                                       cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                     <  1:308923/141‑1 (MQ=255)
                                                                                       cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                     >  2:7046/1‑141 (MQ=255)
                                                                                        tttATACTTGTAGCACTCTTTCTTATGCTGTTTCATTACTATACTAGAAAT‑AAACAATATACACCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGAAATTTCTCAATAAACTTGAAGTaa                                                    >  2:102208/1‑141 (MQ=255)
                                                                                        tttATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACATAT‑ATACATTCTACACCCAAAGGTTAAATTAATGAAAAATAGAATTAAAGATTATAGAAAACAAACAGAAATTTATCAAGAACCTTTAACTaa                                                    >  2:270764/1‑141 (MQ=255)
                                                                                        tttATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaa                                                    >  2:175654/1‑141 (MQ=255)
                                                                                         ttATAATTGTAGCAATATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaaa                                                   <  2:129324/141‑1 (MQ=255)
                                                                                              agtGTAGCAATATTTATTATTCTGTTTAAGTACTATAATAAAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                              <  2:138207/139‑1 (MQ=255)
                                                                                              aTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTCAAGTAAAGAAtt                                              <  2:275805/141‑1 (MQ=255)
                                                                                                 gaagaaatATTTCTTAAGCTGTTGAATTAATATACTAAAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                           <  2:51871/134‑1 (MQ=255)
                                                                                                   aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAg                                         >  1:287095/1‑141 (MQ=255)
                                                                                                      aCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                      >  1:53078/1‑141 (MQ=255)
                                                                                                      aCTATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                      >  2:177495/1‑141 (MQ=255)
                                                                                                      aCTATTCCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGCAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                      >  1:221899/1‑141 (MQ=255)
                                                                                                       agaTTGCTTATGATGTTTAATGAAGAGACGACAAA‑GAAAAAAGATACATGGAAAGGTGTAATTAGTGGTTGGTAGAATTAGAGAATAAAAAAAAGAAAAAGGAAGTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                                     <  2:126299/139‑1 (MQ=255)
                                                                                                        tATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                                    >  2:10163/1‑141 (MQ=255)
                                                                                                        tATTTCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                                    >  2:10188/1‑141 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTCAAGATTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTGCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                                  <  1:215268/141‑1 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTCAAGAATTAAAAGTGTCTAg                                  >  1:252511/1‑141 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                                  <  1:247961/141‑1 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                                  >  2:12082/1‑141 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                                  <  1:204050/141‑1 (MQ=255)
                                                                                                            tCTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGc                                >  1:249435/1‑141 (MQ=255)
                                                                                                            tCTTATGCTGTTTAATTAATATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGc                                <  2:291795/141‑1 (MQ=255)
                                                                                                             cTTATGCTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                               >  1:115589/1‑141 (MQ=255)
                                                                                                             aatATGCAGTTAAATTAATATAATACAAA‑GAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                               <  2:171579/139‑1 (MQ=255)
                                                                                                                  acTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTa                          >  2:88375/2‑141 (MQ=255)
                                                                                                                   ttGTTTAATTACTATAATACAAAT‑AAAAAATATACATGTAAAGGTTAAATCAATGACAAATAGAATTATAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                         <  1:307638/140‑1 (MQ=255)
                                                                                                                   cTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                         >  1:159343/1‑141 (MQ=255)
                                                                                                                   cTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                         >  1:18984/1‑141 (MQ=255)
                                                                                                                   cTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                         >  1:124120/1‑141 (MQ=255)
                                                                                                                   cTGTTTAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                         >  1:18625/1‑141 (MQ=255)
                                                                                                                    tGTTTAATTACTATACTACAAAT‑AAAAAATATACAACTATAGTTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAtt                        <  1:292208/141‑1 (MQ=255)
                                                                                                                      tttAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTaa                      >  2:173665/1‑141 (MQ=255)
                                                                                                                      tttAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAAATTTAAGTAAAGAATTAAAAGTGTCTAGGCAAAATATTaa                      >  2:299655/1‑141 (MQ=255)
                                                                                                                       ttAATTACTATACTACAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAAt                     >  2:136700/1‑141 (MQ=255)
                                                                                                                            aactatactaCAAAT‑AAAAAATATACATATAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt                <  2:120182/140‑1 (MQ=255)
                                                                                                                                atactaCAAAT‑AAAAAATATACATCTAAAAGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaa            <  2:309205/141‑1 (MQ=255)
                                                                                                                                 tactacAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCCAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaaa           <  1:157677/141‑1 (MQ=255)
                                                                                                                                      cAAAT‑AAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaat      >  1:163225/1‑141 (MQ=255)
                                                                                                                                          aaaaaaaaTATACAAATAAAAGTTAAATTAATGAATAATAGAATTAGAGAATATATAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaataaa   <  2:284993/140‑1 (MQ=255)
                                                                                                                                           aaaaaaaaATACAACTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaataaat  <  2:105085/141‑1 (MQ=255)
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TTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAATAAAT  >  CP000731/25524‑25803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑