Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151) – / → repA –/replication protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000731810NG100.0% 172.2 / NA 52intergenic (–/‑151)–/repA–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base G (37/15);  total (37/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220
                                                                                                                                     |                                                                                                                                           
tatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGt                                                                                                                                      >  1:90821‑M1/54‑141 (MQ=255)
  tcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGttt                                                                                                                                    <  2:103553‑M1/90‑1 (MQ=255)
    aagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttat                                                                                                                                  <  1:268962‑M1/92‑1 (MQ=255)
          ttataaacccttgataaagctatatttacccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                            >  2:171037‑M1/44‑141 (MQ=255)
          ttataaacacttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                            >  2:119481‑M1/44‑141 (MQ=255)
           tataaacctctgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                           >  2:172266‑M1/43‑141 (MQ=255)
           tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                           <  2:162230‑M1/99‑1 (MQ=255)
           tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTAAAATAAGCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                           >  2:170988‑M1/43‑141 (MQ=255)
                  ccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCg                                                                                                                    >  2:8556‑M1/36‑141 (MQ=255)
                   cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGa                                                                                                                   >  1:103553‑M1/35‑141 (MQ=255)
                   cttgataaagctatattttcccttttataagtttATTTAAAGTGCCATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGa                                                                                                                   >  1:160016‑M1/35‑141 (MQ=255)
                    ttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGAt                                                                                                                  >  1:24499‑M1/34‑141 (MQ=255)
                     tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATa                                                                                                                 >  1:167843‑M1/33‑141 (MQ=255)
                       ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                               >  1:202265‑M1/31‑141 (MQ=255)
                        taaagctatattttcccttttataagtttGTTTGGAGTGCTATAATAAATTAAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACtt                                                                                                              >  1:43036‑M1/30‑141 (MQ=255)
                        taaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCACAACTACTTGTAATAGACGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTAGATACtt                                                                                                              <  2:78543‑M1/112‑1 (MQ=255)
                          aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTTGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                            >  2:12708‑M1/28‑141 (MQ=255)
                               atattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACCAAAAGCCCAACTACTAGTAACTTTCCGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtattat                                                                                                       >  1:161984‑M1/23‑141 (MQ=255)
                                   ttgcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATaa                                                                                                   >  1:81680‑M1/19‑141 (MQ=255)
                                         ttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaata                                                                                             <  1:229751‑M1/129‑1 (MQ=255)
                                          tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATACTgaatat                                                                                            <  1:224132‑M1/130‑2 (MQ=255)
                                          tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                            >  1:32936‑M1/12‑141 (MQ=255)
                                                 gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGTGTTTGGTAATAAAACGTTTAGAAACTATTTATTTATATAATTTTCGATACTTGTATTATATAATTAATAATATCTTa                                                                                     <  2:69814‑M1/137‑1 (MQ=255)
                                                   ttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGt                                                                                   >  1:253435‑M1/3‑141 (MQ=255)
                                                         aaaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTAGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGCTCCTTGTATTATATAATAAATAATATCTTTGTCCAAGc                                                                             >  2:295155/1‑141 (MQ=255)
                                                          aaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCa                                                                            <  2:82862/141‑1 (MQ=255)
                                                              gCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTa                                                                        >  1:235713/1‑141 (MQ=255)
                                                               cTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTCATAGTCGGGGTTTGGTAATAAGACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTCGTGCAAGCACCTaa                                                                       >  2:221597/1‑141 (MQ=255)
                                                                    ataaACTTAATATAATAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                                  <  2:91447/141‑1 (MQ=255)
                                                                      aaaCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATta                                                                >  1:169962/1‑141 (MQ=255)
                                                                           tAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTACAAGCACCTAAATCATtaaataa                                                           >  2:74549/1‑141 (MQ=255)
                                                                                taACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTa                                                      >  1:25235/1‑141 (MQ=255)
                                                                                  aCAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaaa                                                    >  2:249593/1‑141 (MQ=255)
                                                                                      aaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCta                                                >  1:184264/1‑141 (MQ=255)
                                                                                        aCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGATTAGAAAGTGTTTATTTCTATAATTTTCGATACTTGTATTATATAATGAATAATATCGTAGTACAAGCACCTAAATCATTAAATAAATGTAAAGCtaaa                                              >  2:249458/1‑141 (MQ=255)
                                                                                         ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                             >  1:220069/1‑141 (MQ=255)
                                                                                         ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                             >  1:201931/1‑141 (MQ=255)
                                                                                          cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                            <  1:137398/141‑1 (MQ=255)
                                                                                           ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                           <  1:162230/141‑1 (MQ=255)
                                                                                            cAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataaa                                          >  2:84015/1‑141 (MQ=255)
                                                                                              actactGGTGATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTGTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATaaaaa                                        <  2:111630/141‑1 (MQ=255)
                                                                                                  ctGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAATGCTAAATAAAAACGtt                                    >  1:91136/1‑141 (MQ=255)
                                                                                                   tGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAACTAAATAAAAACGttt                                   >  2:163909/1‑141 (MQ=255)
                                                                                                      tAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAATCACCTAAATTATTATATAAATTTAAAGCTAACTAAAAACGTTttat                                >  1:6763/1‑141 (MQ=255)
                                                                                                       aaTAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAACACGTTttatt                               >  1:3904/1‑141 (MQ=255)
                                                                                                        atatTCGGTGTTTGGTAATAAAATGTTTAGCAACTGTTTAATTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTttatta                              <  2:20785/141‑1 (MQ=255)
                                                                                                         tAGTCGTGGTTTAGTAATAAGAAGTGTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTAc                             <  2:92333/141‑1 (MQ=255)
                                                                                                         tAGTCGTGGGTTGGTAATATGACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCATCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTAc                             <  2:92348/141‑1 (MQ=255)
                                                                                                                    tGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTCCCaaa                  >  2:174636/1‑141 (MQ=255)
                                                                                                                      gTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACt                >  1:185056/1‑141 (MQ=255)
                                                                                                                        aataaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAACTAAAGACCGTTGATGACCTCTTACCCAACTaa              >  1:131226/1‑141 (MQ=255)
                                                                                                                                    aGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAaga  >  1:112474/1‑141 (MQ=255)
                                                                                                                                     |                                                                                                                                           
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑