breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | SNFM_1_70_0_S2283_L006_R1_001.good.fq | 311,310 | 43,858,969 | 100.0% | 140.9 bases | 141 bases | 98.0% |
errors | SNFM_1_70_0_S2283_L006_R2_001.good.fq | 311,310 | 43,858,969 | 100.0% | 140.9 bases | 141 bases | 91.0% |
total | 622,620 | 87,717,938 | 100.0% | 140.9 bases | 141 bases | 94.5% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | USA300TCH1516_ALE | 2,872,915 | 27.3 | 1.5 | 96.0% | Staphylococcus species strain strain. |
coverage | distribution | CP000731 | 27,041 | 72.5 | 1.5 | 2.4% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
coverage | distribution | NC_012417 | 3,125 | 455.2 | 4.4 | 1.6% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
total | 2,903,081 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 976 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 13 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.001 |
reference sequence | pr(no read start) |
---|---|
USA300TCH1516_ALE | 0.91755 |
CP000731 | 0.80740 |
NC_012417 | 0.38695 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
option | value |
---|---|
Mode | Full Polymorphism |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 2 |
Polymorphism frequency cutoff | 0.05 |
Polymorphism minimum variant coverage each strand | 2 |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 15:19:11 23 May 2019 | 15:19:20 23 May 2019 | 9 seconds |
Read alignment to reference genome | 15:19:20 23 May 2019 | 15:21:03 23 May 2019 | 1 minute 43 seconds |
Preprocessing alignments for candidate junction identification | 15:21:03 23 May 2019 | 15:21:10 23 May 2019 | 7 seconds |
Preliminary analysis of coverage distribution | 15:21:10 23 May 2019 | 15:21:30 23 May 2019 | 20 seconds |
Identifying junction candidates | 15:21:30 23 May 2019 | 15:21:30 23 May 2019 | 0 seconds |
Re-alignment to junction candidates | 15:21:30 23 May 2019 | 15:21:49 23 May 2019 | 19 seconds |
Resolving best read alignments | 15:21:49 23 May 2019 | 15:22:00 23 May 2019 | 11 seconds |
Creating BAM files | 15:22:00 23 May 2019 | 15:22:20 23 May 2019 | 20 seconds |
Tabulating error counts | 15:22:20 23 May 2019 | 15:22:26 23 May 2019 | 6 seconds |
Re-calibrating base error rates | 15:22:26 23 May 2019 | 15:22:27 23 May 2019 | 1 second |
Examining read alignment evidence | 15:22:27 23 May 2019 | 15:27:52 23 May 2019 | 5 minutes 25 seconds |
Polymorphism statistics | 15:27:52 23 May 2019 | 15:27:52 23 May 2019 | 0 seconds |
Output | 15:27:52 23 May 2019 | 15:27:55 23 May 2019 | 3 seconds |
Total | 8 minutes 44 seconds |