Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000731	81	N→G	intergenic (–/‑151)	– / → repA	–/replication protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	81	0	N	G	100.0%	188.3 / NA	56	intergenic (–/‑151)	–/repA	–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base G (37/19);  total (37/19)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220                                                                                                                                          |                                                                                                                                            tatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGaaa                                                                                                                                          <  1:65940‑M1/84‑1 (MQ=255)  atttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAAc                                                                                                                                         >  1:249777‑M1/57‑141 (MQ=255)      ttcaagggtttataaacacttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtt                                                                                                                                     >  2:291049‑M1/53‑141 (MQ=255)               ttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAACCGCTTAGACActgtttatttacat                                                                                                                            <  1:96444‑M1/98‑4 (MQ=255)                    aatcattgataaagttatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGTGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                       >  2:188274‑M1/39‑141 (MQ=255)                     acccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAtttt                                                                                                                      >  1:61152‑M1/38‑141 (MQ=255)                        cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGa                                                                                                                   >  1:58198‑M1/35‑141 (MQ=255)                          tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATa                                                                                                                 <  2:25832‑M1/109‑1 (MQ=255)                           gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAc                                                                                                                >  2:93594‑M1/32‑141 (MQ=255)                            ataaagctattttttacttttgagaaatttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                               >  2:277280‑M1/31‑141 (MQ=255)                               aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                            >  1:84373‑M1/28‑141 (MQ=255)                               aagctatatttctcattttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                            >  2:293397‑M1/28‑141 (MQ=255)                                agctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACCGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGta                                                                                                           >  2:12401‑M1/27‑141 (MQ=255)                                    atattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtattat                                                                                                       <  2:18967‑M1/119‑1 (MQ=255)                                      attttccctttaataggtttATGTAAATTGCTATAATAAACTTAATATAACAAAAACCCCAACTAGTTGTAATAGTCGGTGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtatat                                                                                                     >  2:43795‑M1/21‑141 (MQ=255)                                        tttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATaa                                                                                                   >  1:276586‑M1/19‑141 (MQ=255)                                        tttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGATTTGTTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATaa                                                                                                   <  2:194750‑M1/123‑1 (MQ=255)                                          tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATg                                                                                                 >  2:189386‑M1/17‑141 (MQ=255)                                          tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATg                                                                                                 <  2:125034‑M1/125‑1 (MQ=255)                                               tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                            >  1:39996‑M1/12‑141 (MQ=255)                                                 tataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAataata                                                                                          >  2:165485‑M1/10‑141 (MQ=255)                                                   taagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAGTTTTCGATACTTGTATTATATAATGAATAATATc                                                                                        >  2:321721‑M1/8‑141 (MQ=255)                                                       tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAg                                                                                    >  2:299375‑M1/4‑141 (MQ=255)                                                             tAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAg                                                                              >  1:103896/1‑141 (MQ=255)                                                                aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGAAAATAGTCGGGGTTTTGTAATAAAAAGATTAGAAACTGTTTATTTATATAATTTTCGAAAATTGTATTATATAATAAATAATATATTATTAAAAGCaa                                                                           >  2:200027/1‑140 (MQ=255)                                                                  tGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCt                                                                         >  2:18541/1‑141 (MQ=255)                                                                      ataataAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGTGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAAt                                                                     <  2:288511/141‑1 (MQ=255)                                                                        aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTCTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                                   <  1:223358/141‑1 (MQ=255)                                                                         ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                                  <  2:65940/141‑1 (MQ=255)                                                                              cTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaat                                                             <  1:198194/141‑1 (MQ=255)                                                                               ttAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaata                                                            <  1:18967/141‑1 (MQ=255)                                                                                     taACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTa                                                      >  2:285969/1‑141 (MQ=255)                                                                                       tcAAAAACACCAACTACAGGGAATAGTCGGGGTTTGGTAATAAAACGTATAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATTTTAGTGTAAACAACTAAATAATTAAATAAATGTaaa                                                    >  2:150270/2‑141 (MQ=255)                                                                                            aaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTACTGCATGCACCTAAATCATTAAAAAAATGTAACGAtaa                                               >  2:322586/1‑141 (MQ=255)                                                                                             aCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATAAAAAAAACGGTCTAAACTTGTATTATATAATGAATAATATGTTAGTGAAAGCACCTANATCATTGAGTAAATGTATATCtaaa                                              >  1:108088/1‑141 (MQ=255)                                                                                                  aaCTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATaaaa                                         >  1:202490/1‑141 (MQ=255)                                                                                                   actactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTATTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATaaaaa                                        >  2:263606/1‑141 (MQ=255)                                                                                                   actactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAGTGTAAAGCTAAATaaaaa                                        <  1:128292/141‑1 (MQ=255)                                                                                                       ttGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGtt                                    <  2:254635/140‑1 (MQ=255)                                                                                                            aaTAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTttatt                               <  1:74129/141‑1 (MQ=255)                                                                                                                gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTACAGCTAAATAAAAACGTTTTATTACct                           >  2:14944/1‑141 (MQ=255)                                                                                                                   ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCtt                        <  1:51245/141‑1 (MQ=255)                                                                                                                   ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCtt                        >  2:234764/1‑141 (MQ=255)                                                                                                                   ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTAACTCtt                        >  1:259096/1‑141 (MQ=255)                                                                                                                   ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGCACCGCTAAATAAAAACGTTTTATTACCTCtt                        >  1:86876/1‑141 (MQ=255)                                                                                                                      gTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAcc                     <  1:100974/141‑1 (MQ=255)                                                                                                                          ggTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAAc                 <  1:317001/141‑1 (MQ=255)                                                                                                                               taaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGATTAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTCCCAAAATataa            >  2:29240/1‑141 (MQ=255)                                                                                                                                 aaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAata          >  1:297828/1‑141 (MQ=255)                                                                                                                                   atgTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGTACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATg        >  2:316655/3‑141 (MQ=255)                                                                                                                                    cctttAGAAACTGTTTACTTCTATAACTTCCGATACTTGTATTATATAATGAATAATATCTTAGTGCTAGCACCTAAATCATTAAATAAATCTAATCCTAAACAAAAACGCTTTATTACCTCTCACCCAACTacaacaaga       >  2:65492/3‑136 (MQ=255)                                                                                                                                      tttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAACTCATTGAACGAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGaa     >  1:151213/1‑141 (MQ=255)                                                                                                                                        tAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAag   >  1:95943/1‑141 (MQ=255)                                                                                                                                        tAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACATCTTACTAAACTCAAATATGAAAag   >  2:221582/1‑141 (MQ=255)                                                                                                                                         aGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAaga  <  1:126273/141‑1 (MQ=255)                                                                                                                                         aGAAAATGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAaga  <  1:280908/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑