Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,607	T→C	D22G (GAT→GGT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,607	0	T	C	100.0%	76.8 / NA	24	D22G (GAT→GGT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/10);  total (14/10)

TAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTT  >  USA300TCH1516_ALE/1744469‑1744739                                                                                                                                           |                                                                                                                                     tAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATAccc                                                                                                                                    >  1:299449/1‑141 (MQ=255)  aaGCTCCAGGCCAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCt                                                                                                                                   <  1:61514/141‑1 (MQ=255)                    ggagaaaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGtt                                                                                                                 <  1:111791/137‑1 (MQ=255)                         aaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGc                                                                                                            >  1:29539/1‑141 (MQ=255)                                 gtgttCATGTCCTACAATGATGTATTCATCTCTGTATTTTGCGTATTCTACAATTTTATCTGTGGCATAGACATATGCTTCACCATTATCCATAGTTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTg                                                                                                    <  2:233208/138‑1 (MQ=255)                                  gcTTCAGGTCCTAGAATTTTATCAAGATCTCTGTATTTCGAGTGAATGACGATTGTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAAACTTGa                                                                                                   >  1:125203/1‑141 (MQ=255)                                     tCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACt                                                                                                <  1:172814/141‑1 (MQ=255)                                             tACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATAc                                                                                        >  1:251381/1‑141 (MQ=255)                                                     tatCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTTCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCt                                                                                <  2:43657/141‑1 (MQ=255)                                                       tCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCttt                                                                              >  1:315840/1‑141 (MQ=255)                                                           cATCTCTGTCTTTTGCGTATTCTACAATTTTATATGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAAt                                                                          >  1:63619/1‑141 (MQ=255)                                                                                      ttttATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTc                                               <  1:167137/141‑1 (MQ=255)                                                                                          aTCTGTTGCATAGCCATATGCTTTACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTTGGCTAATCTTGAACTTCTGTTACGTATTCCTTTAAATCTATTAATATTTCCTCTTACATTGTTCActc                                           >  2:166418/1‑139 (MQ=255)                                                                                          aTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGa                                           >  1:275563/1‑141 (MQ=255)                                                                                             tGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACaa                                        >  1:147087/1‑141 (MQ=255)                                                                                                    tAGCCATATGTTTCACTATTATTCATAATTGTAGTAGTACCCTTGAAACTATCACCTGATTTCGGCCAATTTTGAACTTATGATACGTTTTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCTCGACAATTGTGAc                                 <  2:6983/141‑1 (MQ=255)                                                                                                                tCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAttt                     >  1:220690/1‑141 (MQ=255)                                                                                                                     aTTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTAt                >  1:92977/1‑141 (MQ=255)                                                                                                                        aTCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTc             >  1:194150/1‑141 (MQ=255)                                                                                                                          ccATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCtt           >  2:142950/1‑140 (MQ=255)                                                                                                                          ccATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTg           <  1:197368/141‑1 (MQ=255)                                                                                                                               aTTGTAGTAATACCCTTGAGTCTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaaaa      <  1:120751/141‑1 (MQ=255)                                                                                                                               aTTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaaaa      <  1:35501/141‑1 (MQ=255)                                                                                                                                 tGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATACAATTTTTTATTTCTGAGACATc    >  2:179730/1‑141 (MQ=255)                                                                                                                                   tagtaATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCtt  >  1:183218/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                     TAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTT  >  USA300TCH1516_ALE/1744469‑1744739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑