| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,884,522 | C→T | 11.1% | A233T (GCC→ACC) | ebh_2 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,884,522 | 0 | C | T | 11.1% | 106.7 / 5.1 | 36 | A233T (GCC→ACC) | ebh_2 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base C (18/14); new base T (2/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CATTGATTAGTGTGAAGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACTTCTCTTT > USA300TCH1516_ALE/1884390‑1884652 | caTTGATTAGTGTGAAGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGAt < 1:163134/141‑1 (MQ=255) ttGATTAGTGTGAAGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCa < 1:34905/141‑1 (MQ=255) tGATTAGTGTGAAGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAg > 1:78562/1‑141 (MQ=255) aaGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACCGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGta < 1:174210/141‑1 (MQ=255) aaGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGta < 2:148019/141‑1 (MQ=255) ccGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGtatacg > 2:124328/1‑139 (MQ=255) ccGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATg > 2:17661/1‑141 (MQ=255) caacGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCaa < 2:128758/141‑1 (MQ=255) cGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTg > 2:133663/1‑141 (MQ=255) tttACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGTATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGt > 1:99189/1‑141 (MQ=255) tttACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGTATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGt > 1:43922/1‑141 (MQ=255) tACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGc > 1:134586/1‑141 (MQ=255) cTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGcac < 1:180628/141‑1 (MQ=255) cTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGcac < 1:73613/141‑1 (MQ=255) tGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCaccacc < 1:107856/141‑1 (MQ=255) tGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCaccacc < 1:82281/141‑1 (MQ=255) aaCGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGTGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTa > 1:106287/1‑141 (MQ=255) aaCGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTa > 2:133214/1‑141 (MQ=255) aaCGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTa > 2:175732/1‑141 (MQ=255) aaCGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTa > 2:175705/1‑141 (MQ=255) ggTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGc < 2:79773/141‑1 (MQ=255) ggTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGc < 2:123896/141‑1 (MQ=255) tACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGCCCTGCGTTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAg < 1:206940/141‑1 (MQ=255) aTACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATct > 1:58422/1‑141 (MQ=255) tACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATctg > 1:88249/1‑141 (MQ=255) aTTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTgg > 1:198902/1‑141 (MQ=255) gTTAGGTGCATTTTGTCCTGCATTATTGGTATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGc < 2:43922/141‑1 (MQ=255) gTTAGGTGCATTTTGTCCTGCATTATTGGTATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGc < 2:99189/141‑1 (MQ=255) tGCATTTTGTCCTGCATTATTGGCATTAGGTTCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGGACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACg > 2:154762/1‑141 (MQ=255) tGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACg > 2:186317/1‑141 (MQ=255) tGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACg > 2:223526/1‑141 (MQ=255) tGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACtt < 2:259791/141‑1 (MQ=255) cTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACTTctct < 2:78562/141‑1 (MQ=255) gCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACTACAGAGTCCTACTACTGCATCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACTTCTCttt > 1:247478/1‑141 (MQ=255) gCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACTTCTCttt > 1:187198/1‑141 (MQ=255) gCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACTTCTCttt > 1:216236/1‑141 (MQ=255) | CATTGATTAGTGTGAAGCCCGGCAAGTTATTAACAACGTTTACTGTTGGCACAGAACGGTTGGTACTTGGTCTAATACCATTGTCATCAAATGACAGCACTTCGTTAGGTGCATTTTGTCCTGCATTATTGGCATTAGGATCAGTTGTTGGCGTATATGGCGCTGTAATTGCAACTGGTGCACCACCGTTTCCTACCGCTGCAGCCGCTGGATCTGCTGGAGTTGCATTAGGATCTGCCGGTGCCGCACGTCTACTTCTCTTT > USA300TCH1516_ALE/1884390‑1884652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |