| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,484,799 | G→A | 10.2% | A295V (GCG→GTG) | mqo1 ← | putative malate:quinone oxidoreductase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,484,799 | 0 | G | A | 10.2% | 117.0 / 4.8 | 39 | A295V (GCG→GTG) | mqo1 | putative malate:quinone oxidoreductase 1 |
| Reads supporting (aligned to +/- strand): ref base G (20/15); new base A (2/2); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
TTGGTTTAACCGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATCCCAGTCTTTTGGA > USA300TCH1516_ALE/2484659‑2484931 | ttGGTTTAACCGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGgcg < 2:161584/141‑1 (MQ=255) tAACCGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCa > 1:232111/1‑141 (MQ=255) aCCGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTATTTGCGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGAGATTGGTGGCGCACCCAct > 1:77522/1‑141 (MQ=255) ccGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCActg < 2:22246/141‑1 (MQ=255) ccGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCActg < 1:188283/141‑1 (MQ=255) aTTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCActgctg > 2:47889/1‑141 (MQ=255) tttAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCACCTAACCATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACtgctgc < 1:25898/141‑1 (MQ=255) tttAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACtgctgc > 1:116184/1‑141 (MQ=255) tttAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACtgctgc < 2:202293/141‑1 (MQ=255) tttAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACtgctgc > 2:12107/1‑141 (MQ=255) gtgAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGCCATTGCTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAg > 2:29748/1‑141 (MQ=255) tttttAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGAc > 2:172182/1‑141 (MQ=255) ttAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCACACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGat > 2:182796/1‑141 (MQ=255) ttAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCACACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGat > 2:126687/1‑141 (MQ=255) aaaaTTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGatcaa > 2:109538/1‑141 (MQ=255) aTTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGatcaatca > 1:31792/1‑141 (MQ=255) aTTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGatcaatca < 1:99058/141‑1 (MQ=255) aTTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGatcaatca > 1:90845/1‑141 (MQ=255) ttAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCAct < 1:256961/141‑1 (MQ=255) aTGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCa < 2:223005/141‑1 (MQ=255) ggACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTCTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAgg < 1:193258/141‑1 (MQ=255) aaCGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCg > 1:221357/1‑141 (MQ=255) aaCGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCg > 1:212216/1‑141 (MQ=255) aCGCTTGCCGTCTACAAAGCGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCgg < 1:60560/141‑1 (MQ=255) gCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCTCAGGAATAATCCACTTACCGGGa < 1:126791/141‑1 (MQ=255) cTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGaa < 1:47889/141‑1 (MQ=255) ttGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGaaa > 1:209320/1‑141 (MQ=255) ttGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGaaa > 1:222162/1‑141 (MQ=255) tGCCGTCTACAAAGAGTGTATCTAAGTGCTGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATTATTACCATCAATCACGTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAac < 2:77522/141‑1 (MQ=255) aCGTGTATCTAAGTGCGGCACTGACATTGGTGGCACACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGttt < 1:182796/141‑1 (MQ=255) aCGTGTATCTAAGTGCGGCACTGACATTGGTGGCACACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGAAGGCATAATCCACTTACCGGGAAACCACCAATATGttt < 1:126687/141‑1 (MQ=255) tATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGAtt < 1:17045/141‑1 (MQ=255) gCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATcc > 2:187482/1‑141 (MQ=255) gCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATcc > 2:190092/1‑141 (MQ=255) gCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATcc > 2:126218/1‑141 (MQ=255) cGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATccc > 2:149872/1‑141 (MQ=255) gCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATCCCAg < 1:109538/141‑1 (MQ=255) aCTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATCCCAGTc < 1:172182/141‑1 (MQ=255) ttGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATCCCAGTCTTTTGGa > 2:190595/1‑141 (MQ=255) ttGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATCCCAGTCTTTTGGa > 1:37993/1‑141 (MQ=255) | TTGGTTTAACCGATTTAATTAAATCCATATGTGAACCTGTTTTTAAAAATTTAGGTGAGAAACCTGCAAATGGACCAAATAACAATGAACGCTTGCCGTCTACAAAACGTGTATCTAAGTGCGGCACTGACATTGGTGGCGCACCCACTGCTGCTTTTCCGTACACTTTAGCATGATGACGATCAATCACTTCTTGATTTGTACAGCGCAGGAATAATCCACTTACCGGGAAACCACCAATATGTTTTGATTGTTTAATCCCAGTCTTTTGGA > USA300TCH1516_ALE/2484659‑2484931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |