| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,279,705 | G→A | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,279,705 | 0 | G | A | 97.5% | 111.6 / NA | 40 | A943V (GCA→GTA) | ebh_3 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/24); minor base T (0/1); total (15/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC > USA300TCH1516_ALE/2279570‑2279838 | gtgCCGTTATTAGTTGGTTCTACTGCTGGTTTACTAGTTACAGCGTTATCCATTGTCTGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgtt < 2:169706/141‑1 (MQ=255) tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTAATGAATCTACTCTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgctt > 2:209910/1‑138 (MQ=255) tccGTTTTTCGTTGTTTCTTCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATTTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgtt‑gt < 2:185528/140‑1 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTg < 1:346309/141‑1 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTg < 2:178625/141‑1 (MQ=255) aTTCTAGTTCGGGTTTACTAGTTACATCGTTACCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCg < 1:27543/141‑1 (MQ=255) aTTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCg < 1:111565/141‑1 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACCCTCGTTGTCTTATTAGCTCGTGGTGGACTATTTGCATCATCAGTTGCTGAGGTTACTGTT‑GTTTCACCTGTTGCTGCATCAc > 2:246175/1‑141 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCAc < 1:321319/141‑1 (MQ=255) tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACtt > 2:14698/1‑140 (MQ=255) tCTGGTGTAGTAGTTACATCGTTATCCTTTGTCGGCCTGTTTGTTGATGCTTCTACAGTAGAATTGTCATTAGCTTGCGGTTTATCTTTTGCATCATCAGTTGCTGTTATTACTGTT‑GTTTCACCTGTTGCCGCATCACta < 2:305828/141‑1 (MQ=255) tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGCT‑GTTTCACCTGTTGCCGCATCACtatt > 1:18406/1‑141 (MQ=255) tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACTTGTTGCCGCATCACTattatt > 2:312635/1‑141 (MQ=255) ttAGTTACATCGTTATCCTTTGTCGGACTGTTTGTTTATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGgt < 2:128109/140‑1 (MQ=255) gtAGTTATATTGTTTTTTATTGTGGGACTGTTTGTTGATGGATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGgt < 2:333850/140‑1 (MQ=255) cTAGTTACATCGTTATCCAGTGTAGGATTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGgt < 2:129755/141‑1 (MQ=255) gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTGTCGgaga > 1:338568/1‑141 (MQ=255) ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa > 1:259523/1‑141 (MQ=255) ttATCCATTGTCGGACAGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgagca > 1:310992/1‑139 (MQ=255) gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt < 1:110271/141‑1 (MQ=255) gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTCTATCACCCTGAGCATCAGTTGTTGCTGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt < 2:336416/141‑1 (MQ=255) aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc > 1:197882/1‑141 (MQ=255) ttttgATGCATCTACACTAGAATTGTGTTTATCTTGCGGTTTCTCATTTGCATCATCAGTTGCTGATGTTAATGTTCGTGTC‑CGTGTTGGTTTAACGCTATTATTTGGTGTCGTCGGAGAAGCGTTTGCTTTGCCATTAGc < 2:74698/139‑1 (MQ=255) tgttgATGCTTCTACATTAGAATTGTTATTAGCTTTCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 2:152173/141‑1 (MQ=255) cATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct < 2:197882/141‑1 (MQ=255) acacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGAt > 1:286448/1‑141 (MQ=255) gAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTCCCATTAGCTGTCGTCCCAGATACCttt > 2:64808/1‑140 (MQ=255) taGCTTGCTGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg < 1:51022/141‑1 (MQ=255) taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg < 1:105864/141‑1 (MQ=255) tttATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTTGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgt > 2:267067/1‑140 (MQ=255) ttATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt < 1:302618/141‑1 (MQ=255) ttATCATTTGCATCATCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt < 1:73602/141‑1 (MQ=255) tttGCATCAGCAGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCGGGATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCa < 2:338568/141‑1 (MQ=255) atcaGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAgaaaa > 2:223964/1‑137 (MQ=255) tcaGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt > 1:84008/1‑141 (MQ=255) caGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTA‑TTTCTGGTGTTGCATTAGCATTTGa < 2:342793/141‑1 (MQ=255) aGTTGCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa < 1:223964/141‑1 (MQ=255) gCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAttt < 1:340498/141‑1 (MQ=255) gCTGATGTTACTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGAGTTGTCGGAGAAGCGTCTGCTTTGCCATTAACTATTTTCACAAATACATTAGGTTGTCCAATATATTCTGGTGTTGCATTATCATTTGAAttt > 2:227967/1‑141 (MQ=255) tGATGTGTCTGTT‑GTTTGACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc < 2:128343/141‑1 (MQ=255) | GTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTT‑GTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC > USA300TCH1516_ALE/2279570‑2279838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |