BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,202,470	G→A	10.0%	V483I (GTT→ATT)	ileS →	Isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,202,470	0	G	A	10.0%	123.7 / 4.7	40	V483I (GTT→ATT)	ileS	Isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (16/20); new base A (2/2); total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GGGGTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACGTTTG  >  USA300TCH1516_ALE/1202330‑1202606

ggggTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATg                                                                                                                                          <  1:154840/141‑1 (MQ=255)

ggggTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATg                                                                                                                                          <  1:153177/141‑1 (MQ=255)

               tttACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTg                                                                                                                           <  2:101591/141‑1 (MQ=255)

               tttACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTg                                                                                                                           <  2:192879/141‑1 (MQ=255)

                           ttCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGtt                                                                                                               <  2:436849/141‑1 (MQ=255)

                             cGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGTGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCa                                                                                                             <  1:237007/141‑1 (MQ=255)

                                  ccGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAatat                                                                                                        >  2:254568/1‑141 (MQ=255)

                                  ccGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAatat                                                                                                        >  2:186413/1‑141 (MQ=255)

                                  ccGTGGCGAATGGGTTATTTCTCGTCAACGTGTCTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAatat                                                                                                        >  1:239683/1‑141 (MQ=255)

                                                  aTTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAATCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGaa                                                                                        <  1:253763/141‑1 (MQ=255)

                                                  aTTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGaa                                                                                        <  1:284648/141‑1 (MQ=255)

                                                  aTTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGaa                                                                                        <  1:28718/141‑1 (MQ=255)

                                                      ctcGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGa                                                                                    <  1:284294/141‑1 (MQ=255)

                                                        cGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGaaa                                                                                  >  2:187427/1‑141 (MQ=255)

                                                        cGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGaaa                                                                                  >  2:220478/1‑141 (MQ=255)

                                                                tgtgtgGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTtact                                                                          >  2:442870/1‑141 (MQ=255)

                                                                tgtgtgGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTtact                                                                          >  1:377256/1‑141 (MQ=255)

                                                                tgtgtgGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTACAAATATTTGGTTTGAAAGAGAAGCGAAAGACTtact                                                                          >  2:303012/1‑141 (MQ=255)

                                                                          gtACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGa                                                                <  2:53822/141‑1 (MQ=255)

                                                                          gtACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGa                                                                >  1:96527/1‑141 (MQ=255)

                                                                          gtACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGa                                                                >  1:74745/1‑141 (MQ=255)

                                                                            aCCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGAtt                                                              <  1:423423/141‑1 (MQ=255)

                                                                                     aGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACAAGAAGGATTTACACATcc                                                     >  2:445686/1‑141 (MQ=255)

                                                                                     aGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATcc                                                     <  1:186413/141‑1 (MQ=255)

                                                                                     aGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATcc                                                     <  1:254568/141‑1 (MQ=255)

                                                                                            tATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGc                                              >  2:328830/1‑141 (MQ=255)

                                                                                            tATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTAGGTTTGCAAGAGAAGCGAAAGACTTCCTCCCAGAAGGATTTACACATCCAGGCAGc                                              >  2:262484/1‑141 (MQ=255)

                                                                                                 tGAAAATGTCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAGATATTTGGTTTGAAAGAGAAGCGAGAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTaa                                         <  1:395860/141‑1 (MQ=255)

                                                                                                 tGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTaa                                         <  1:440743/141‑1 (MQ=255)

                                                                                                  gAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAAc                                        <  1:55488/141‑1 (MQ=255)

                                                                                                  gAAAATGACGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAAc                                        <  2:276944/141‑1 (MQ=255)

                                                                                                       tGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTAc                                   >  2:329330/1‑141 (MQ=255)

                                                                                                             aaTTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTAc                             <  2:316326/141‑1 (MQ=255)

                                                                                                                 aTCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTaaa                         <  2:246754/141‑1 (MQ=255)

                                                                                                                 aTCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTaaa                         <  2:189496/141‑1 (MQ=255)

                                                                                                                 aTCATGACGAAAGAAACAGTGAATCACGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTaaa                         <  2:364941/141‑1 (MQ=255)

                                                                                                                            agaaaCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACAt              >  1:27845/1‑141 (MQ=255)

                                                                                                                            agaaaCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACAt              >  1:44325/1‑141 (MQ=255)

                                                                                                                                aCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATg          <  1:329793/141‑1 (MQ=255)

                                                                                                                                aCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATg          <  1:47279/141‑1 (MQ=255)

                                                                                                                                     gAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACgt     >  2:239438/1‑141 (MQ=255)

                                                                                                                                        tCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACgtttg  >  2:326995/1‑141 (MQ=255)

GGGGTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACGTTTG  >  USA300TCH1516_ALE/1202330‑1202606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`