Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,567 T→C 5.3% D1223D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5670TC 5.3% 930.2 / 8.3 398D1223D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (172/181);  new base C (25/4);  total (207/191)
Fisher's exact test for biased strand distribution p-value = 7.11e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

GATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTC  >  USA300TCH1516_ALE/618427‑618707
                                                                                                                                            |                                                                                                                                            
gattccgacgccgattcagaccgcgactcagattcagacagCGACTCAGCCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagat                                                                                                                                              <  1:893017/136‑1 (MQ=17)
   agcgactcagataccgacagtgacgcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattca                                                                                                                                           <  2:2924484/141‑1 (MQ=255)
     cgattcagactcagatagcgactcagactcagacagCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattcaga                                                                                                                                         <  1:3998246/141‑1 (MQ=255)
      gactcagattcagacagcgactcagattcagacagcGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACGGCGACTCAGACTCAGAGAGCGACTCAGACACAGAAAGAGAAtcagagtcagaa                                                                                                                                        >  2:2687674/1‑140 (MQ=14)
        ctcagattcagacagcgactcagattcagacagcgaCTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAg                                                                                                                                      <  1:3615634/141‑1 (MQ=255)
          cagactcagacagcgactcagattcagacagcgactCAGAATCAGACAGTTGTTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCg                                                                                                                                    <  1:825142/141‑1 (MQ=14)
           agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGa                                                                                                                                   >  1:3546315/1‑141 (MQ=255)
           agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAATCAGCCTCAGACGGCGAATCAGACTCAGAGAGCGACTCAGCATCAGAGAGCGa                                                                                                                                   >  2:3314270/1‑141 (MQ=14)
              ttcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTc                                                                                                                                <  1:3712634/141‑1 (MQ=255)
               tcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCa                                                                                                                               >  1:2826081/1‑141 (MQ=255)
                 agacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGa                                                                                                                             <  1:1058963/141‑1 (MQ=255)
                      gcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGGGACGCAGACGCAGAGGGCGACGCAGGCTCAGATAGTGACTCAGAGTGAGATAGCGACTCAGATTggg                                                                                                                        >  1:3065423/1‑141 (MQ=17)
                       cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                                       >  1:2730658/1‑141 (MQ=255)
                          ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                    <  1:3347792/141‑1 (MQ=255)
                          ctcagactcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                    <  1:4187087/141‑1 (MQ=255)
                           tgagattcagagagagaatgagaTGAAGAGAGTGAGTGAGACTCAGATGGCGACGCAGATTCAGAGCGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGc                                                                                                                   <  2:656412/139‑1 (MQ=14)
                           tcagattcagacagcgactcagaCTCAGATAGTGCCTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGAGAGCGACTCAGCTGCAGACaac                                                                                                                   >  2:185440/1‑139 (MQ=14)
                           tcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCGGACGGCGACTCAGAGTCGGATAGCGACTCGGAGTCGGAGAGTCACTCAGACGCGGATAGCGAGCCAGAGTCGGACGGc                                                                                                                   >  2:2138262/1‑141 (MQ=14)
                           tcagattcagacagcgactcagaCTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCCACTCAGACTcagctacc                                                                                                                   >  2:833053/1‑134 (MQ=11)
                               attcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                               >  1:1578392/1‑141 (MQ=255)
                                   agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGAGACTCAGATTCAGACAGCGACACAGACTCAGACAGCGACTCAGACTCACAGAGGGCCACGGATTCACATAACGACACAGACTCGGAAAGCGAGTCAGa                                                                                                           >  2:856971/1‑141 (MQ=17)
                                      cagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                                                                        <  1:1296659/141‑1 (MQ=255)
                                       agcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGCTAGCGACTCAGATTCGGACAGCGATTCAGACTcc                                                                                                       >  1:1525637/1‑140 (MQ=255)
                                       agcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                                                                       >  1:318507/1‑141 (MQ=255)
                                          gactcagactcaGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTGAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGATTCAGATAGAAACTCAAACACGCACAGCGATTCAGACTGAGAt                                                                                                    >  2:2380601/1‑141 (MQ=14)
                                          gactcagactcaGATAGTGATTCAGACTCAGATAGCGACCGAAAGACAGACAGGGACTGAGACAGAGACAGCGACAGAGAATAAGACAGTGACCGCGATACCGATAAAGACACAGACTCAGACAGCGACACAGACTCAGac                                                                                                    >  2:1477343/1‑140 (MQ=2)
                                            ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                  <  1:3933796/141‑1 (MQ=255)
                                            ctcagactcagaGAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                  <  1:2110519/141‑1 (MQ=255)
                                              cagacacagacaGTGAGTCAGACTCAGATAGCGACTCAGATTCAGACCGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                                                                <  1:377990/141‑1 (MQ=255)
                                              aagactaagaTAGTGATTCAGATTCAGATAGCGTCTCAGATTCAGACAGTGACTCAGACTCAGACCGCGACTCAGACTCAGATAGTGTCTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                                                                <  2:4284405/140‑1 (MQ=14)
                                               tacctccgaTAGAGATATCGACTCAGATAGCGACTGCGATTCAGACAGCGACTCAGAGTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                               <  2:3228994/138‑1 (MQ=14)
                                                gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                              <  1:3437198/141‑1 (MQ=255)
                                                gactcagaTAGCGATTCAGACTCAGCTTCCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                              <  2:1209099/141‑1 (MQ=14)
                                                  ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                            <  1:1170/141‑1 (MQ=255)
                                                    cagaGAGTGATTCAGACTCAGATAGAGACTCAGATTCAGACAGCGAAACAGACTCAGACAGCGACGAAGACTCAGAAAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag                                                                                          <  1:602016/141‑1 (MQ=14)
                                                        tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTAAGGTAGCGACtcagattg                                                                                      >  1:4132331/1‑140 (MQ=255)
                                                          gTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATCCAGACTCAGATAGCAACtcagattcag                                                                                    >  2:2755364/1‑141 (MQ=255)
                                                              ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  1:3299042/141‑1 (MQ=255)
                                                              ttCAGACTCAGATAGCCACTCAAATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  1:4305943/141‑1 (MQ=255)
                                                              ttCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  1:807526/141‑1 (MQ=255)
                                                               tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc                                                                               >  1:3242920/1‑141 (MQ=255)
                                                                caagacgaGATAGGTATTCAGATTCATACAGAGACGAAGACTCGGACAGGGACTCAGACTCAGATAGTGACTCAGATGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                              <  2:3116140/134‑1 (MQ=14)
                                                                  gACTCAGATAGTGACTCAGATTCAGACAGCGACTCAGACTCAGACATCTACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                            <  1:771104/141‑1 (MQ=255)
                                                                   aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGCTTCATAGTTATTTTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtt                                                                           <  1:1031456/141‑1 (MQ=14)
                                                                       aGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                       <  1:3883343/141‑1 (MQ=255)
                                                                       aGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                       <  1:2351953/141‑1 (MQ=255)
                                                                        gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                      <  1:3489939/141‑1 (MQ=255)
                                                                          aaGCGACATAGACGCAGACAGCGAAGTAGCTTCATAGAGACACTCAGACTCAAATGGTGACGAAGATTCAGATATCGACTTAGATTCGGACAGCGAATAATATCCAGCCACCAACGCTTATTCAGATAGCGATTCGGACTc                                                                    <  2:2021511/140‑1 (MQ=2)
                                                                            gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                  <  1:2901239/141‑1 (MQ=255)
                                                                                gtCAGAGTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                              <  2:3519014/140‑1 (MQ=255)
                                                                                ctaaGATTGAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                              <  2:1022017/138‑1 (MQ=255)
                                                                                cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                              >  1:3968393/1‑141 (MQ=255)
                                                                                   ggcttCAGACAGCGACGCAGCCTCAGCCAGAGACTCAGCCTCAGACCGCGACACAGCTTCAGATCGCGCCTCCGGCTCGGACAGCGAGTCAGACTCAGATAGCGCCCGGGCATCGGAGAGCGATTCGGACTCAGATAGCGa                                                           <  2:3847839/138‑1 (MQ=12)
                                                                                   agagTCAGACAGCGACACAGAGTCAGACAGCGACTCAGACTTAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                           <  2:3405037/141‑1 (MQ=255)
                                                                                     aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCgccc                                                         >  1:1412986/1‑138 (MQ=255)
                                                                                     aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                         >  1:56057/1‑141 (MQ=255)
                                                                                       tcagacagcgactcagactcagacagcgactcagacTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtca                                                       <  1:246623/141‑1 (MQ=255)
                                                                                        cagtcagcgactcagactcagacagcgactcagactCAGATAGTGACGCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCTATTCGGACTCAGATAGCGACtcag                                                      <  1:4046365/141‑1 (MQ=255)
                                                                                        cagacagcgactcagactcagacagcgactcagactCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGGCAGCGATTCAGACGCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                      >  1:1450641/1‑141 (MQ=255)
                                                                                        cagacagcgactcagactcagacagcgactcagactCAGATAGCGACTCAGACTCAGCTAGCGACTCAGACTCGGCCAGCGAATCAGACTCACATAGCGACTCAGAACCATCTAGCGAGTCGGAATCAGCTAGCGagacaa                                                      >  2:1609957/1‑136 (MQ=11)
                                                                                         agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                                     <  1:2264117/141‑1 (MQ=255)
                                                                                          gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACCCAGATAGCGAAtcagca                                                    >  2:2284007/1‑139 (MQ=14)
                                                                                          aacagcgactcagaatcagacagcgactcagacacagATAGTGGCTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                    <  1:648106/140‑1 (MQ=255)
                                                                                            cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                  >  1:3903976/1‑141 (MQ=255)
                                                                                            cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACCCAGATTCAGATAACGAATCGCACCCAGATAGCGACtccgaggg                                                  >  1:2708089/1‑138 (MQ=14)
                                                                                            cagcgacgcagacacagacagcgactccgactcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                  <  1:3470356/141‑1 (MQ=255)
                                                                                             agcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGCTTCAGATAGCGATTCGGACTCAGATAGCGACtcagattca                                                 >  1:1206971/1‑141 (MQ=255)
                                                                                             agcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattca                                                 >  1:1964497/1‑141 (MQ=255)
                                                                                             agcgactcagactcagaaagcgacgcagactcagaCAGAGAATCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattca                                                 <  2:4391889/141‑1 (MQ=14)
                                                                                              gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGCTAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagagtccg                                                >  1:409911/1‑139 (MQ=255)
                                                                                                  ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                            <  1:185440/141‑1 (MQ=255)
                                                                                                    cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCCGACAGCGATTCAGACTCAGATAGCGACTGAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGACTCAGATAgcg                                          >  2:1571969/1‑139 (MQ=255)
                                                                                                     aaactcagactgcgattcagattcagaTAGTGATTCAGATTCAGATAGCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                         <  1:1145315/139‑1 (MQ=14)
                                                                                                           agacagcgtctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                   <  1:2504493/141‑1 (MQ=255)
                                                                                                           agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAGACAGTTAGTGGTTCAgg                                   >  1:1658683/1‑140 (MQ=255)
                                                                                                           agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCACACTCAGATAGCGACTCAGAACCACATAGTGATTCAgg                                   >  2:1326444/1‑140 (MQ=17)
                                                                                                            gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                                  <  1:2207852/141‑1 (MQ=255)
                                                                                                              cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                <  1:4218066/141‑1 (MQ=255)
                                                                                                               agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                               >  1:480438/1‑141 (MQ=255)
                                                                                                                 cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                             >  1:2988786/1‑141 (MQ=255)
                                                                                                                  gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGAt                            >  1:1551197/1‑141 (MQ=255)
                                                                                                                    gtcagaatgagaCAGCGACTGAGATTCAGACAGCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGATTCAGATAGCGACTCAGATTTAGATAGTGATTCAGACTCAGATAg                          <  2:301203/140‑1 (MQ=11)
                                                                                                                    ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                          <  1:975633/141‑1 (MQ=255)
                                                                                                                     tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGc                         >  1:724088/1‑141 (MQ=255)
                                                                                                                      cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGGTAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg                        >  1:2598506/1‑141 (MQ=255)
                                                                                                                        gactcagaTAGTGACTCAGATTCAGAGAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                      <  1:2941711/141‑1 (MQ=255)
                                                                                                                           tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                   >  1:1947326/1‑141 (MQ=255)
                                                                                                                               aTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACGCAGacc               >  2:396501/1‑139 (MQ=11)
                                                                                                                                tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTc              <  1:4249858/141‑1 (MQ=255)
                                                                                                                                tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATACAGAAAGTGATTCAGACAGAGAAAGCGACTCAAAtac              >  2:2806689/1‑139 (MQ=14)
                                                                                                                                tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGAGATTCAGACTGAGAAAGCGACTCAGATTCAGAGAGCGACTCGGACTCAGCTAGCGGATTAGAATGAGATAGCGATTCACACCCCGCCAGCGACTCAGaagc              >  2:4175406/1‑138 (MQ=2)
                                                                                                                                 agagACGCAGCTTCAGATAGAGACTGAGATTCAGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa             <  2:1655452/138‑1 (MQ=14)
                                                                                                                                 aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTGAGATCGTGATTCAGCCTCAGATAGCGCGTCAGATTcc             >  1:1608749/1‑140 (MQ=14)
                                                                                                                                 aGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATCCACACACTGATAGCAAAACAGACTCa             >  2:1081338/1‑141 (MQ=11)
                                                                                                                                  gTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTAAGAATCAGCGAGCGACGCAgcgtcaa            >  2:557802/1‑135 (MQ=11)
                                                                                                                                   tGACTCAGATTCAGATAGCGACTCAGATTCGGACAACGGTTCAAACTGAGAGAGCGACTCAGATTCAGATAGCGATTCTGAATAACGGAAGGGCGCAGATTCAGAAAACGATTCAGACACGGATAGCGACCCCGGTTCAGa           >  2:729140/1‑141 (MQ=2)
                                                                                                                                           attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt   >  1:2422044/1‑141 (MQ=255)
                                                                                                                                           attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTCAGATAGCGCCACAGATTCACACCGCGAc  >  2:2547596/1‑140 (MQ=17)
                                                                                                                                           attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGAc  >  2:3181280/1‑140 (MQ=255)
                                                                                                                                           attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTAGGACTCAGATAGCGACTCAGATACAAATAGTGATTCACACTCAGATAGCGCCCCAGAATCACCCAGAGAc  >  2:367154/1‑140 (MQ=14)
                                                                                                                                            |                                                                                                                                            
GATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTC  >  USA300TCH1516_ALE/618427‑618707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: