Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,621 T→C 5.1% D1241D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6210TC 5.1% 970.4 / 7.9 404D1241D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (193/173);  new base C (23/3);  total (226/178)
Fisher's exact test for biased strand distribution p-value = 3.33e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

TAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTC  >  USA300TCH1516_ALE/618483‑618761
                                                                                                                                           |                                                                                                                                            
tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTAAGGTAGCGACtcagattg                                                                                                                                            >  1:4132331/1‑140 (MQ=255)
  gTGATTCCGACTCAGATAGCGACACAGATTCAGACAGCGCCTCAGACTCAGACAGCAATAACCCGGAGAAGAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcag                                                                                                                                          <  1:3231310/141‑1 (MQ=14)
   tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagaatcaga                                                                                                                                         >  1:2585245/1‑141 (MQ=255)
      ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                                      <  1:737784/141‑1 (MQ=255)
      ttCAGACTCAGATAGCCACTCAAATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                                      <  1:4305943/141‑1 (MQ=255)
       tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGAATCCGACACAAATAGAGAATCAGATTAAGATAGc                                                                                                                                     >  2:3945056/1‑141 (MQ=14)
        caagacgaGATAGGTATTCAGATTCATACAGAGACGAAGACTCGGACAGGGACTCAGACTCAGATAGTGACTCAGATGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                                                                                    <  2:3116140/134‑1 (MQ=14)
         aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                                                                                   <  1:1988228/141‑1 (MQ=255)
          gACTCAGATCGCGACTCCGAGTCAGACAGCGACTCAGACTCAGCAAGCGCCTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCCGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                                                                                  <  1:1438954/141‑1 (MQ=14)
          gACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                                                                                  <  1:3912766/141‑1 (MQ=255)
           aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGCTTCATAGTTATTTTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtt                                                                                                                                 <  1:1031456/141‑1 (MQ=14)
           aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGagt                                                                                                                                 >  1:2183413/1‑139 (MQ=255)
           aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCGGAAGGAGAGGAGGGCTCAGGTAGCGACTCAGATTCAGATGGAGAtt                                                                                                                                 >  1:116908/1‑141 (MQ=14)
               aGATAGCGACTCAGATTCAGACGGAGACTGAGACTCAGACAGCGACTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                             <  2:2763654/141‑1 (MQ=14)
                gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                            <  1:3489939/141‑1 (MQ=255)
                  tAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                          <  1:2902376/141‑1 (MQ=255)
                  tAGAGACTCAGATTTAGATAGAGAATCAGACTCAGACAGAGACTCAGATTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                          <  2:2950040/141‑1 (MQ=14)
                  aggCGACTCAGATTGAGACAGCGACGCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                          <  2:849523/139‑1 (MQ=255)
                        gtCAGAGTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    <  2:3519014/140‑1 (MQ=255)
                        cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    >  1:3968393/1‑141 (MQ=255)
                          cAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                  <  1:3799148/141‑1 (MQ=255)
                           agagTCAGACAGCGACACAGAGTCAGACAGCGACTCAGACTTAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                 <  2:3405037/141‑1 (MQ=255)
                           aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                 >  1:975768/1‑141 (MQ=255)
                            gATTCAGCGCGCGAAGCAGACACAGACAGCGACACAGAATCAGATAGAGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                <  2:2401498/141‑1 (MQ=14)
                             aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                                                                               >  1:602051/1‑141 (MQ=255)
                             aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAGTCAGACTCAGATAGCGCCTCAGAATCACATGGCGATTCGAACTCAGATAgagccc                                                                                                               >  2:1588704/1‑136 (MQ=11)
                                cagacagcgactcagactcagacagcgactcagactCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                                                                            <  1:4001808/141‑1 (MQ=255)
                                 agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagg                                                                                                           >  1:2726167/1‑140 (MQ=255)
                                 agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                                                                                           <  1:2820873/141‑1 (MQ=255)
                                 agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                                                                                           <  1:3313265/141‑1 (MQ=255)
                                  gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                                                          >  1:3078000/1‑141 (MQ=255)
                                   caagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagagt                                                                                                         >  1:1931176/3‑139 (MQ=255)
                                      gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGCTAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagagtccg                                                                                                      >  1:409911/1‑139 (MQ=255)
                                       cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGGCGCGGCTTCGGACGGCGATTCAGACTCAGATAGCGAATCATATTCAGATAGCGATTCGGACTCAGATCGAGACtcaggttcaga                                                                                                     >  2:2731457/1‑141 (MQ=17)
                                       cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                                                     >  1:3876663/1‑141 (MQ=255)
                                            cgcgcgcagccagcgactcagactcagtGAGAGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                                                                                <  2:2121970/135‑1 (MQ=14)
                                             agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                               <  1:2313169/141‑1 (MQ=255)
                                              gactcagacagcgactcagactcagaTAGTGCATCACACCCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAATCAGCTTCAGATAGCTACTCGTGCTCAAATAGCGTCTAAAATGCAAATAATGAt                                                                                              >  2:2021282/1‑141 (MQ=9)
                                              gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATGCAGATAGTGAt                                                                                              >  1:2210714/1‑141 (MQ=255)
                                              gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGATAGTGATTCGGAGTCAGATAGCGACTCAGAGTCAGAGCGTGag                                                                                              >  2:2233185/1‑140 (MQ=17)
                                               actcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                             >  1:3277777/1‑141 (MQ=255)
                                               actcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                             >  1:1556867/1‑141 (MQ=255)
                                                   agacagcgtctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                         <  1:2504493/141‑1 (MQ=255)
                                                   agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                         >  1:3440900/1‑141 (MQ=255)
                                                    gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCGGACAGCGATTCCGACTCAGACAGCGACTCAGATTCAGATCGCGATACAGACTCCCATCGCGACTCAGACTCACATAGCGACTCAGAc                                                                                        >  2:1636390/1‑141 (MQ=14)
                                                      cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                      <  1:1541035/141‑1 (MQ=255)
                                                      cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                      <  1:3670590/141‑1 (MQ=255)
                                                       agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                     >  1:2403047/1‑141 (MQ=255)
                                                       agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGATAGCGGTCGAGACACCGATAGAGACACAGATACAGATAGTGATTCAGaacca                                                                                     >  2:149055/1‑137 (MQ=17)
                                                        gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGAACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATACTGATTCAGACTCAg                                                                                    >  1:2456417/1‑141 (MQ=255)
                                                         cgactcagactcagaTAGTGACTCAGATTCAGCTCGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAATCAGACGCAGATAGCGAATCAGATTCACATAGAGAAAGAGACTCaaa                                                                                   >  2:4359485/1‑139 (MQ=14)
                                                         cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                   >  1:2988786/1‑141 (MQ=255)
                                                         cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCCGGTAGCGACTCCGGTTCAGGAAGTGATTTAGACTCAGa                                                                                   >  2:58645/1‑141 (MQ=17)
                                                          gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAAAGCGACTCAGACGAAGATAGCGCGTCAGACGCAGAt                                                                                  >  2:702402/1‑141 (MQ=14)
                                                              cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACACAGACTCAGATAGCGATTCAGACTCAGATacag                                                                              >  2:1744004/1‑138 (MQ=14)
                                                                  tgcagaTACTGACGCAGATTCAGACGGCGAGTGAGATTCGGAGAGCGATTCAGTGTTGTATGTGTACTCGGACTCAGAGAGCGACACGGACTCAGATAGCGGCTCAGAGTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                          <  2:1373564/139‑1 (MQ=9)
                                                                  ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                          >  1:3480570/1‑141 (MQ=255)
                                                                          aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGAGCCTAAGAGGCAGAGAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                                   >  1:822891/1‑141 (MQ=17)
                                                                               cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                              <  1:908798/141‑1 (MQ=255)
                                                                               cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGAGATTCAGACTCAGGTAGCGACTCAGATTCAGaaaa                                                              >  2:790929/1‑138 (MQ=14)
                                                                                  agattcagatAGCCATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                           <  2:1227644/141‑1 (MQ=255)
                                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                         >  1:792262/1‑141 (MQ=255)
                                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                         >  1:3538555/1‑141 (MQ=255)
                                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                         >  2:3062775/1‑141 (MQ=255)
                                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTCAGATAGCGCCACAGATTCACACCGCGAcc                                                         >  2:2547596/1‑140 (MQ=17)
                                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGAca                                                         >  2:3181280/1‑140 (MQ=255)
                                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTAGGACTCAGATAGCGACTCAGATACAAATAGTGATTCACACTCAGATAGCGCCCCAGAATCACCCAGAGAcg                                                         >  2:367154/1‑140 (MQ=14)
                                                                                     ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGGCAGCGACTc                                                        >  1:496101/1‑141 (MQ=255)
                                                                                     ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc                                                        <  1:3110691/141‑1 (MQ=255)
                                                                                      tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCa                                                       <  1:1348716/141‑1 (MQ=255)
                                                                                        agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                     >  1:42365/1‑141 (MQ=255)
                                                                                           tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTc                                                  >  1:4073174/1‑141 (MQ=255)
                                                                                            aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCACACAGCGACTCAGATTCg                                                 >  1:1135654/1‑141 (MQ=255)
                                                                                            aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTTCGATTCAGACAACGAAACAGATTCg                                                 >  2:3734169/1‑141 (MQ=14)
                                                                                             gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCgg                                                >  1:2854010/1‑141 (MQ=255)
                                                                                              cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGa                                               >  1:3734397/1‑141 (MQ=255)
                                                                                                 ctgaGAATCGGACAGCGATTCATGCTCAGATTTCTATTTATTTTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACGCAGATAGAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                            <  1:68323/138‑1 (MQ=17)
                                                                                                 cTCAGATTCGGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                            <  1:1595948/141‑1 (MQ=255)
                                                                                                  tCAGATTCGGATTGCGAGTCAGACTCAGATCGGGACTCAGATTCAGACAGCGATTCCGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                           <  2:1162295/141‑1 (MQ=14)
                                                                                                  tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                           <  1:4259072/141‑1 (MQ=255)
                                                                                                  tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                           >  1:2771707/1‑141 (MQ=255)
                                                                                                    agagTCAGACAGCGATTCAGCATCAGATCGCGACTCAGAGTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGa                                         <  2:1868352/141‑1 (MQ=17)
                                                                                                    aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCgc                                         >  1:1386331/1‑140 (MQ=255)
                                                                                                     gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTCAGATAGCGACCCAGATTCACACAGCAACTCAGATTCAGATAGCgcc                                        >  2:4220815/1‑139 (MQ=17)
                                                                                                       ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTc                                      <  1:419375/141‑1 (MQ=255)
                                                                                                         tgggcggCGATTCAGACGCAGATAGCGACTCATATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAg                                    <  2:383717/135‑1 (MQ=255)
                                                                                                          ggACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGCTAGCGACTCAGAGTCAGATAGTGAATCAGACTCAGATAGCGACTCAGCTTCAGACAGCGACTCAAATACGAAAAGCGACTCAGa                                   >  2:3328309/1‑141 (MQ=17)
                                                                                                           gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                                  <  1:1614513/141‑1 (MQ=255)
                                                                                                              aGCGATGCAGGCTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                               <  1:2659086/141‑1 (MQ=255)
                                                                                                                    tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc                         >  1:55917/1‑141 (MQ=255)
                                                                                                                    tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATCCAGACAGCGACTCAGATTCGAATAGCCACTCAGATAcagccagc                         >  2:4015358/1‑141 (MQ=17)
                                                                                                                      aGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa                       <  1:1744485/141‑1 (MQ=255)
                                                                                                                       gACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCCGATAGCAACTCAGACACAAACAGCCAc                      >  2:3259055/1‑141 (MQ=14)
                                                                                                                         cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                    <  1:2954121/141‑1 (MQ=255)
                                                                                                                         cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAGTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                    <  1:867571/141‑1 (MQ=255)
                                                                                                                          tCAGATAGCGACTCAGATTCAGATAGCGATTCAGATTCTGGTGGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCa                   <  1:1206228/141‑1 (MQ=17)
                                                                                                                           cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                  <  1:4403419/141‑1 (MQ=255)
                                                                                                                               ttgCGACTGGGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc              <  2:4100092/139‑1 (MQ=255)
                                                                                                                                  cGACTCAGATTCAGATTGCGGTTCTTACACAGCTTGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa           <  2:489906/141‑1 (MQ=18)
                                                                                                                                         gattcagatAGCGAGTCGGACTACGACAGCGACTCAGAGTCATATAGGGATACAGGCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt    <  2:865869/141‑1 (MQ=17)
                                                                                                                                           |                                                                                                                                            
TAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTC  >  USA300TCH1516_ALE/618483‑618761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: