Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,657 T→C 6.9% D1253D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6570TC 6.9% 864.6 / 9.1 394D1253D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (207/135);  new base C (21/12);  total (238/156)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 440 total aligned reads displayed.

GACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTC  >  USA300TCH1516_ALE/618517‑618797
                                                                                                                                             |                                                                                                                                            
gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                                                                                              >  1:3078000/1‑141 (MQ=255)
  cagcgactcagactcagacagcgactaagactcagaTAGTCACTCAGATTCAGATAGAGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                                                                            <  1:4212207/141‑1 (MQ=255)
  cagcgacgcagacacagacagcgactccgactcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                                                                            <  1:3470356/141‑1 (MQ=255)
    gcgactcagattcagacggagactcagactcagaTAGCGATTGAGATCAAGATACCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                                                                                                          <  2:3127445/141‑1 (MQ=14)
    gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                                                                                                          <  1:716080/141‑1 (MQ=255)
    gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                                                                                                          <  1:3096260/141‑1 (MQ=255)
     cgactcagactcagacagcgtctgacgcgcagaTAGTTACGCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                                                                                         <  1:1385260/141‑1 (MQ=14)
     cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGGCGCGGCTTCGGACGGCGATTCAGACTCAGATAGCGAATCATATTCAGATAGCGATTCGGACTCAGATCGAGACtcaggttcaga                                                                                                                                         >  2:2731457/1‑141 (MQ=17)
        ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                                      <  1:185440/141‑1 (MQ=255)
          gagactcagagagcgacgcagactcagaGACGGACACAGACTAGGAGAGCGACTCAGATTCGGATAGTGATTGAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                                                                                                                    <  2:843640/140‑1 (MQ=14)
           agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                                                                   <  1:2313169/141‑1 (MQ=255)
             actcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                                                                 >  1:1556867/1‑141 (MQ=255)
              ctcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTc                                                                                                                                <  1:4270062/141‑1 (MQ=255)
               tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGCCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATGCa                                                                                                                               >  2:2175793/1‑141 (MQ=255)
                  gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGCTTCAGATAGTGATTCAGAc                                                                                                                            >  1:2465006/1‑141 (MQ=255)
                  gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                                                                                                                            <  1:2207852/141‑1 (MQ=255)
                    cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                                          <  1:3670590/141‑1 (MQ=255)
                     agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                                                         >  1:2826729/1‑141 (MQ=255)
                       cgactcagactcagaTAGTGACTCAGATTCAGCTCGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAATCAGACGCAGATAGCGAATCAGATTCACATAGAGAAAGAGACTCaaa                                                                                                                       >  2:4359485/1‑139 (MQ=14)
                       cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                                       <  1:1548507/141‑1 (MQ=255)
                       cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCCGGTAGCGACTCCGGTTCAGGAAGTGATTTAGACTCAGa                                                                                                                       >  2:58645/1‑141 (MQ=17)
                        gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAAAGCGACTCAGACGAAGATAGCGCGTCAGACGCAGAt                                                                                                                      >  2:702402/1‑141 (MQ=14)
                          ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                                                                                                    <  1:674839/141‑1 (MQ=255)
                            cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGGTAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg                                                                                                                  >  1:2598506/1‑141 (MQ=255)
                               actcagaTAGTGAGTGAGATTCAGACAGGGACTCAGAGTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACt                                                                                                               <  2:342546/141‑1 (MQ=14)
                                tgcagaTACTGACGCAGATTCAGACGGCGAGTGAGATTCGGAGAGCGATTCAGTGTTGTATGTGTACTCGGACTCAGAGAGCGACACGGACTCAGATAGCGGCTCAGAGTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                                                              <  2:1373564/139‑1 (MQ=9)
                                 tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                                                                                             >  1:1947326/1‑141 (MQ=255)
                                   agaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa                                                                                                           >  1:3802680/1‑141 (MQ=255)
                                       aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGAGCCTAAGAGGCAGAGAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                       >  1:822891/1‑141 (MQ=17)
                                               agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                               <  2:320847/141‑1 (MQ=255)
                                               agattcagatAGCCATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                               <  2:1227644/141‑1 (MQ=255)
                                                 attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                                             >  1:2422044/1‑141 (MQ=255)
                                                 attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                                             >  1:3974642/1‑141 (MQ=255)
                                                 attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAAAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGCGatc                                                                                             >  2:1054872/1‑139 (MQ=255)
                                                    cagatAGCGACTCAGATTCGGACAGCGATTCAGACTAAGGACCGGCGCCAGAGTCAGGTTGTGGGAAACACCCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                                          >  1:4393139/1‑141 (MQ=9)
                                                     agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                                                         >  1:42365/1‑141 (MQ=255)
                                                     agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGAGAcacact                                                                                         >  1:350214/1‑139 (MQ=255)
                                                      gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGAACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGCTTCAGACAGCGACTCAGac                                                                                        >  2:3587561/1‑140 (MQ=255)
                                                         aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                                     <  1:524733/141‑1 (MQ=255)
                                                         aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCACACAGCGACTCAGATTCg                                                                                     >  1:1135654/1‑141 (MQ=255)
                                                          gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCgg                                                                                    >  1:2885559/1‑141 (MQ=255)
                                                           cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGa                                                                                   >  1:3734397/1‑141 (MQ=255)
                                                            gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAt                                                                                  <  1:2547323/141‑1 (MQ=255)
                                                             aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCggctc                                                                                 >  1:2042919/1‑138 (MQ=255)
                                                              cTCAGATTCGGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                                                                <  1:1595948/141‑1 (MQ=255)
                                                               tCAGATTCGGATTGCGAGTCAGACTCAGATCGGGACTCAGATTCAGACAGCGATTCCGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                                               <  2:1162295/141‑1 (MQ=14)
                                                               tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                                               >  1:4084724/1‑141 (MQ=255)
                                                                cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGTTTCTGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCg                                                                              >  1:3180979/1‑141 (MQ=255)
                                                                  gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTCAGATAGCGACCCAGATTCACACAGCAACTCAGATTCAGATAGCgcc                                                                            >  2:4220815/1‑139 (MQ=17)
                                                                    ctcgtagaGCGATTGAGATTCTGATAGCGTGTGAGATTCGGATAGCGACTAGGACTCAGCTAGCGACTCAGATTCAGATAGTGATTCAGAGTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTc                                                                          <  2:1518147/134‑1 (MQ=17)
                                                                       ggACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGCTAGCGACTCAGAGTCAGATAGTGAATCAGACTCAGATAGCGACTCAGCTTCAGACAGCGACTCAAATACGAAAAGCGACTCAGa                                                                       >  2:3328309/1‑141 (MQ=17)
                                                                        gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                                                                      <  1:1614513/141‑1 (MQ=255)
                                                                           aGCGCTACAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                                                   <  1:4131986/141‑1 (MQ=255)
                                                                                ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTCAGAAAGCCCCCCATCAGCATAGAGCGACACAGACTCAACTACCGACTCAGACTCAGACAg                                                              >  2:1988749/1‑141 (MQ=9)
                                                                                   aGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa                                                           <  1:1744485/141‑1 (MQ=255)
                                                                                     aCTCAGATGGAGACGCAGATTCAGATAGAGATTCCGACTCAGATAGAGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACt                                                         <  2:3047775/141‑1 (MQ=17)
                                                                                     aCTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACACAAATTAGGCTAACAACTCAGATTCACACCGCGACt                                                         >  2:1766597/1‑141 (MQ=17)
                                                                                      cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                                                        <  1:2954121/141‑1 (MQ=255)
                                                                                      cTCAGATAGCGACTAAGATTCAGATAGCGACGCGGACTCAGATAGCGACTCAGATTCAGAGAGAGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                                                        <  2:4159392/141‑1 (MQ=17)
                                                                                        cAGATTGCGACGCTGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                                      <  2:3981069/141‑1 (MQ=14)
                                                                                         aGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                     >  1:1301988/1‑141 (MQ=255)
                                                                                         aGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                     <  1:3847456/141‑1 (MQ=255)
                                                                                            tAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc                                                  >  1:2750644/1‑141 (MQ=255)
                                                                                               cGACTCAGATTCAGATTGCGGTTCTTACACAGCTTGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                                               <  2:489906/141‑1 (MQ=18)
                                                                                               cGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                                               <  1:3335864/141‑1 (MQ=255)
                                                                                                  cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAg                                            <  1:904848/141‑1 (MQ=255)
                                                                                                    tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                                           >  1:1039245/1‑141 (MQ=255)
                                                                                                    tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGagcga                                           >  2:1411657/1‑137 (MQ=255)
                                                                                                      agattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGACTCAGACTCAGATAGCAACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCTGATTCAGACAGCGACTCAGACTCGGATAGTGa                                         <  2:3903976/141‑1 (MQ=255)
                                                                                                       gattcagatAGCGAGTCGGACTACGACAGCGACTCAGAGTCATATAGGGATACAGGCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                                        <  2:865869/141‑1 (MQ=17)
                                                                                                         ctcagatAGCTATCCAGACTCAAAGAGCGACCCACAATCGGAAAGTGAGCCAGACTCGGATAGCGACTCAGACACAGACAGCGACCCAGATTCGGATAGCGACTCAGATTCAGACAGAGCCTCAGACTCGGATAGTGATTc                                      <  2:1612328/140‑1 (MQ=9)
                                                                                                           cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGGTGGTGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg                                    >  1:3010267/1‑141 (MQ=255)
                                                                                                           cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATCGTGATTCAg                                    >  1:849277/1‑141 (MQ=255)
                                                                                                           cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTCAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg                                    <  1:2380601/141‑1 (MQ=255)
                                                                                                           cagagAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACGTCGACTCAGATTCGGATTGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg                                    <  1:1477343/141‑1 (MQ=255)
                                                                                                            agatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGa                                   <  1:94365/141‑1 (MQ=255)
                                                                                                               tAGCGATTCGGACGCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                <  1:3911908/141‑1 (MQ=255)
                                                                                                                 gCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGCCTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAg                              >  1:447695/1‑141 (MQ=255)
                                                                                                                   gagtCGGACTCTGATTGTAAGACAGATTCAGATAGGGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGAt                            <  2:3277777/138‑1 (MQ=17)
                                                                                                                     ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                          >  2:279840/1‑141 (MQ=255)
                                                                                                                     ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAg                          >  2:3829888/1‑141 (MQ=255)
                                                                                                                        ggACTCAGATATAGACTCAGAGTCAGAGCGAGAATCAGACTCAGATAGGGACTCAGATTCAGATAGCGACTCAGAGTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCga                       <  2:2286546/141‑1 (MQ=14)
                                                                                                                        ggACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCga                       <  1:358353/141‑1 (MQ=255)
                                                                                                                          aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgact                     >  1:2670079/1‑141 (MQ=255)
                                                                                                                           cTCAGATAGTGCTTCTGATTCAGATAGCGAGTCAGACTCAGATAGCGCCTCAGACTCAGATAGCGACTCAGACTCCGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                    <  2:2530490/141‑1 (MQ=14)
                                                                                                                           cTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                    <  1:790929/141‑1 (MQ=255)
                                                                                                                            tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactca                   >  2:4080308/1‑141 (MQ=255)
                                                                                                                            tCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACACCCACTCACACTCGGATAGTGACTCCGACTCAACTAACgactcc                   >  2:766477/1‑140 (MQ=17)
                                                                                                                             cAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcag                  >  1:4315733/1‑141 (MQ=255)
                                                                                                                                  aGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACACCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactca             >  2:2430385/1‑141 (MQ=255)
                                                                                                                                       cTCAGATTCACATACTGACTCAGCCTCAGACAGCGACTCTGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg        <  2:2968886/141‑1 (MQ=18)
                                                                                                                                        tcagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGc       <  1:28416/141‑1 (MQ=255)
                                                                                                                                          agattcagatAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACACAGATTCGGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGCATAGTGAATCAGACTCAGATAGCGACTCAGACTCACATAGcaa     >  2:1812323/1‑139 (MQ=17)
                                                                                                                                           aattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgat    <  1:3253871/140‑1 (MQ=255)
                                                                                                                                            attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATATCGACCCAGATTCAGACAGCCACTCAGACTCGGATAGTGCTTCAGACTCAGATAGCCACTCACACTCCGATAGTgat  >  2:2913550/1‑140 (MQ=18)
                                                                                                                                            attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGAGTCAGACTCAGATAGCgatt   >  1:1190936/1‑141 (MQ=255)
                                                                                                                                            attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgatt   >  1:4017865/1‑141 (MQ=255)
                                                                                                                                            attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATACAGACACAGACTCACATAGCgatt   >  2:1664668/1‑141 (MQ=17)
                                                                                                                                            attcagatAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGCGACTCAGACACAGATAGCgat  >  2:3716766/1‑140 (MQ=17)
                                                                                                                                             ttcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattc  <  1:3419418/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTC  >  USA300TCH1516_ALE/618517‑618797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: