BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,660	T→A	13.8%	I173I (ATT→ATA)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,660	0	T	A	13.8%	1198.5 / 141.1	460	I173I (ATT→ATA)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (150/243); new base A (31/33); total (181/279)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 473 total aligned reads displayed.

CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTT  >  USA300TCH1516_ALE/110521‑110800

  gtaaaaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCa                                                                                                                                           <  1:2363383/138‑1 (MQ=255)

   ttAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGCAACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                                          >  2:4206091/1‑141 (MQ=255)

     aaaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                                        <  1:3505274/141‑1 (MQ=255)

         tGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAAtt                                                                                                                                    >  1:3115316/1‑140 (MQ=255)

                   aaaaaTCATACCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                          <  1:601285/140‑1 (MQ=255)

                    aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                                         <  1:807658/141‑1 (MQ=255)

                    aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                                         >  1:2508315/1‑141 (MQ=255)

                     aaaTCATTCCAACAAAAGACATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                        >  2:4090962/1‑141 (MQ=255)

                     aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                        <  1:1498533/141‑1 (MQ=255)

                      aaTCATTCCAACAAAAGAAAAAAAAAAAAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                                       <  2:776498/141‑1 (MQ=17)

                       ataactCCAACAAAAGGAATTAAAGATCAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATGTGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                                      <  1:2799284/136‑1 (MQ=255)

                            tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTc                                                                                                                 >  1:782846/1‑141 (MQ=255)

                             ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCg                                                                                                                <  1:1584649/141‑1 (MQ=255)

                             ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCg                                                                                                                <  1:3944730/141‑1 (MQ=255)

                               aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                              >  1:1180894/1‑141 (MQ=255)

                                acaaAAGAAATTAAAGATGAAAAAATAAAAAACGAAATCGAAAACTTTAAGTTCTTTGTTCAATATAGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGTAGATATTTAATATAATCAAGATGTGTCGATTTATACGGCt                                                                                                             >  2:895769/1‑141 (MQ=17)

                                acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                             <  1:4069826/141‑1 (MQ=255)

                                 caaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                                            <  1:3419623/141‑1 (MQ=255)

                                   gatctaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGACTaaa                                                                                                          >  2:3947815/6‑141 (MQ=255)

                                    aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGATGCAGAGTTATTCAGCTAAat                                                                                                         >  2:509623/1‑141 (MQ=255)

                                      gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGGCTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                       >  1:4250922/1‑141 (MQ=255)

                                       aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATTCGGCTAAATATc                                                                                                      >  1:4283235/1‑141 (MQ=255)

                                        aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                     >  2:3990486/1‑141 (MQ=255)

                                         aTTAAAGATGAAAAAATAAAAAAAGAAATAGAAACAATTAAGTACTATGTTCAATATGGCGACATTAAA‑AAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATGCAAAGGTAAAGAGTTATTCAGATTAATATGaa                                                                                                    >  2:3603166/1‑141 (MQ=17)

                                            aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                 >  1:1400577/1‑141 (MQ=255)

                                             aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                >  1:543620/1‑141 (MQ=255)

                                             aaGATGAAAAAATAAAAAAAACAATGGAAAACGTTAAGGTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                >  1:861410/1‑141 (MQ=18)

                                              aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                               <  1:671159/141‑1 (MQ=255)

                                               gATGAAAGAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTACAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                              <  2:330689/141‑1 (MQ=255)

                                               gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                              <  2:1423205/141‑1 (MQ=255)

                                               gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTAGGCGAAATATTAATTCAca                                                                                              >  2:4372312/1‑140 (MQ=17)

                                                aagAAAAAATAAAAAAAAAAATCGAAAAATTTAAGTTTTATGAGCAATATACCAATTCCAAAGAATTTATCATAAT‑TAAAGATAGAGATATATCATATAATCCAAAGGCATTTATTTATTCGGCTAAATATCAATTAACTa                                                                                             <  2:1148773/139‑1 (MQ=2)

                                                aTGAAAAAGTAAAAAAAGAAATCGAAAACTTTAAGTTCTGTGTGCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                             <  1:1845733/141‑1 (MQ=255)

                                                aTGAAAAAACAAAAAAAGAAATCGAAAACTTTAAGCTCTTTCTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                             <  1:3728085/141‑1 (MQ=255)

                                                  gAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                           >  1:3783789/1‑141 (MQ=255)

                                                   aaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTATGATCAATATGGCGACTTTAAA‑AATTTTAAAAATTATAAAGACGGAGATATTTCATATAATCTAAAGATACCGAGTTATTCGGATAAATATCAATTAACTAatg                                                                                          >  2:3805588/1‑141 (MQ=17)

                                                     aaaaaaaaaaaaGAAATCGAAAAATTTAAGTTCTTTGTTCAATATCGCGACTTTAAA‑AAATTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                        <  2:1290930/141‑1 (MQ=255)

                                                     aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGAGTAAATATCAATTAACTAatgat                                                                                        >  1:1656470/1‑141 (MQ=255)

                                                        aTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                     >  2:2886245/1‑141 (MQ=255)

                                                           aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                  <  1:569200/141‑1 (MQ=255)

                                                            aaaaaGAAATCGAAAACTTTAAGTTCATTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                                 >  2:1934820/1‑141 (MQ=255)

                                                              aaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                               >  2:1774059/1‑141 (MQ=255)

                                                               aaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                              <  1:183193/141‑1 (MQ=255)

                                                                  aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                           <  1:4150317/141‑1 (MQ=255)

                                                                  aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                           <  1:2185938/141‑1 (MQ=255)

                                                                  aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                           <  1:2409332/141‑1 (MQ=255)

                                                                        aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                     <  2:570094/141‑1 (MQ=255)

                                                                           aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                  <  1:1344416/141‑1 (MQ=255)

                                                                           aCTGTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                  <  1:1976134/141‑1 (MQ=255)

                                                                              ttAAGTTCTTTGTTCAATATGTCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                               <  2:180369/141‑1 (MQ=255)

                                                                              ttAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                               <  1:96100/141‑1 (MQ=255)

                                                                              tgaaGTTCTGTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                               <  1:2072603/139‑1 (MQ=255)

                                                                                  gTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAGGTAGTAGAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                           <  2:2167774/141‑1 (MQ=14)

                                                                                    tCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                         <  1:453626/141‑1 (MQ=255)

                                                                                       ttGTTCAATATGGCGACTTTAAA‑ACTTTGAAAAATTATAACGACGGAAATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatg                                                      <  1:3670078/141‑1 (MQ=255)

                                                                                       ttGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatg                                                      <  1:2582016/141‑1 (MQ=255)

                                                                                            cAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                                 >  2:2039308/1‑141 (MQ=255)

                                                                                                atGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACtga                                             <  1:4054763/141‑1 (MQ=255)

                                                                                                  ggCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAATAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                           >  2:2416825/1‑141 (MQ=255)

                                                                                                   gCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAATAGATTGGATATACcgacga                                          >  2:475055/1‑141 (MQ=255)

                                                                                                   gCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcaacac                                          >  1:3257606/1‑139 (MQ=255)

                                                                                                       ttttAAA‑GATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                      <  2:1183619/140‑1 (MQ=11)

                                                                                                       cTTTAAA‑AAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                      <  2:3515860/141‑1 (MQ=255)

                                                                                                        tttAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGATATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                                     >  2:3051114/1‑141 (MQ=255)

                                                                                                            aa‑aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                                 <  1:4090962/141‑1 (MQ=255)

                                                                                                            aa‑aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                                 >  2:668634/1‑141 (MQ=255)

                                                                                                             a‑aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                                <  2:4152065/141‑1 (MQ=255)

                                                                                                               aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                               >  2:171812/1‑141 (MQ=255)

                                                                                                               aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                               >  1:1750139/1‑141 (MQ=255)

                                                                                                                    gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                          >  1:1870927/1‑141 (MQ=255)

                                                                                                                     atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                         >  1:3595716/4‑141 (MQ=255)

                                                                                                                     atgaaTTAAAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                         <  1:2673237/138‑1 (MQ=255)

                                                                                                                       gaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAGAAATTTGATATACCGATGAATAAAGCTCCAAAattatt                       >  1:1119304/2‑141 (MQ=14)

                                                                                                                         aTTATAAAGATGGAGATATCTAAAATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt                     <  2:3294811/141‑1 (MQ=18)

                                                                                                                         aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTGATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt                     <  1:1011969/141‑1 (MQ=255)

                                                                                                                          ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGCTTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt                    >  1:2907364/1‑141 (MQ=255)

                                                                                                                          ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt                    <  2:4286180/141‑1 (MQ=255)

                                                                                                                          atataAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGATACATATCCCTTAACTACTGATCATTATAATGTAAAACAATTACGAAAAATACATGCTATACCGCAGGGTACCGCTCCAAAGTTATTGtt                    <  2:2946672/140‑1 (MQ=14)

                                                                                                                           tataAAGATGGAGATATATCATATAATACAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATAGTTa                   <  2:3243612/141‑1 (MQ=255)

                                                                                                                            ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa                  <  2:820626/141‑1 (MQ=255)

                                                                                                                            ataAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa                  <  1:10509/141‑1 (MQ=255)

                                                                                                                             taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa                 >  2:1588785/1‑141 (MQ=255)

                                                                                                                             taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa                 <  1:3878838/141‑1 (MQ=255)

                                                                                                                             taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa                 <  1:1952454/141‑1 (MQ=255)

                                                                                                                               aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg               >  1:354201/1‑141 (MQ=255)

                                                                                                                                aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTCAAAAGTTATGATATACCGACTAGTAAAGCTCCAAAGTTATTGTTAAAAgg              <  1:2103944/141‑1 (MQ=255)

                                                                                                                                aGACGGAGATATTTCATATAATCCAGAGGTGACGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg              <  2:31403/141‑1 (MQ=255)

                                                                                                                                 gACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTTAGTAATTGTGATTATGATATAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt             <  2:2002885/141‑1 (MQ=21)

                                                                                                                                  atggAGATATATCATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt            <  2:3677826/139‑1 (MQ=255)

                                                                                                                                  aaggAGATATAACATAAAAACCAGAGTTGTCGAGTTATTCAGCAAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt            <  2:2646771/139‑1 (MQ=17)

                                                                                                                                    ggAGATATTTCATATAATCCAGAGGTGACGAGATATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATTTGATATACCGACGAGTAAAGCTCCAAAGTTATTGGTAAAATGttaa          >  2:1866683/1‑139 (MQ=18)

                                                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAATGCTGCCAATCTAGTGTTAAAAGGTTCAggg       <  2:1391979/141‑1 (MQ=17)

                                                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg       >  2:3424702/1‑141 (MQ=255)

                                                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg       <  2:3933319/141‑1 (MQ=255)

                                                                                                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGa      <  1:2315565/141‑1 (MQ=255)

                                                                                                                                        atatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGa      >  2:1054669/1‑141 (MQ=255)

                                                                                                                                         tatTTCATATAATAAAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGGAAAAGATATGATATATCGAAGAGAAAAACTACAAAGTTATTGTTAAAAAGTACAGGaaa     >  2:2512368/1‑141 (MQ=17)

                                                                                                                                          atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAACGCTCCAAAATTATTGTTACGAGGTTCAGGGCAt    >  1:127008/1‑141 (MQ=18)

                                                                                                                                           tttCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAACGCTACAAAGTTATTGTTAAAAGGTTCAGGGAAtt   <  1:3048698/141‑1 (MQ=255)

                                                                                                                                           tttCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAtt   <  1:2031588/141‑1 (MQ=255)

CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTT  >  USA300TCH1516_ALE/110521‑110800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`