Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,028,652 C→T 9.6% intergenic (‑8/+7) USA300HOU_RS10230 ← / ← USA300HOU_RS10235 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,028,6520CT 9.6% 681.5 / 50.6 274intergenic (‑8/+7)USA300HOU_RS10230/USA300HOU_RS10235hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (126/115);  new base T (17/10);  total (149/125)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

Only 100 of 288 total aligned reads displayed.

AAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTAATAT  >  USA300TCH1516_ALE/2028512‑2028792
                                                                                                                                            |                                                                                                                                            
 aaCGAACACACAACCCAACAACAGAACGGGTAAAAGACCAATTTTTTCAAAAAATATGTACTAAAAACGGGCACGCCGCTGGAATGCAGTAAAATTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTcgccg                                                                                                                                             <  1:412038/141‑1 (MQ=255)
   cTAAGACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCgccgcc                                                                                                                                           <  1:3357753/141‑1 (MQ=255)
       tACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTc                                                                                                                                       <  1:2792552/141‑1 (MQ=255)
       tACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAAGGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTc                                                                                                                                       <  2:1418796/141‑1 (MQ=255)
           caTCCCAATAATAGAACGAGTAAAACGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATACAGTAAAACTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTCTg                                                                                                                                   >  2:1582281/1‑141 (MQ=255)
           caTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATg                                                                                                                                   >  1:3958224/1‑141 (MQ=255)
             tctcAAGAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGcc                                                                                                                                 <  1:3999962/138‑1 (MQ=255)
             tCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGcc                                                                                                                                 <  1:3702293/141‑1 (MQ=255)
             tCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGcc                                                                                                                                 <  2:4185955/141‑1 (MQ=255)
             ccccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGcc                                                                                                                                 <  1:1122544/140‑1 (MQ=255)
               ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAACCGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcac                                                                                                                               >  1:1897761/1‑141 (MQ=255)
               ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcac                                                                                                                               >  1:3570729/1‑141 (MQ=255)
               ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTAATGTTTCGCAGCCTTTCTATGCcac                                                                                                                               >  1:500261/1‑141 (MQ=255)
                cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGAcaca                                                                                                                              >  2:3912875/1‑141 (MQ=255)
                 aataatAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                                                                                             >  2:2793612/1‑141 (MQ=255)
                  ataataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGc                                                                                                                            >  2:3158561/1‑141 (MQ=255)
                  ataataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTAGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGc                                                                                                                            >  2:3043311/1‑141 (MQ=255)
                  ataataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAAGTAGCGATAATCATTCAGGCAGTAAAAACGATAAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTCTGCCCCAGc                                                                                                                            >  1:1041058/1‑141 (MQ=255)
                    aataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGt                                                                                                                          <  1:2453210/141‑1 (MQ=255)
                      taGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATAGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCAGTGCGTTc                                                                                                                        >  2:301436/1‑141 (MQ=255)
                        gAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATCTATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCat                                                                                                                      <  1:966376/141‑1 (MQ=255)
                        gAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCat                                                                                                                      <  1:2904588/141‑1 (MQ=255)
                         aaCGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCAGCCTTTCTATGCCAAAGCGTTAata                                                                                                                     >  1:2167309/1‑141 (MQ=255)
                         aaCGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACACTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCata                                                                                                                     <  2:209385/141‑1 (MQ=255)
                          aCGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCatat                                                                                                                    >  1:3461622/1‑141 (MQ=255)
                              gTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAtt                                                                                                                <  1:2675274/141‑1 (MQ=255)
                               aaaaaaGCAAATTTTGTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAttt                                                                                                               <  2:1022380/140‑1 (MQ=255)
                                aaaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGTAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATtta                                                                                                              >  2:1388753/1‑141 (MQ=255)
                                 aaagGCGAATTTGTTCAATAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATGTGCGTTGATATATAATTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATttat                                                                                                             <  2:2114848/141‑1 (MQ=255)
                                 aaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACGGCGTTCATATAATttat                                                                                                             >  1:312741/1‑141 (MQ=255)
                                 aaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATttat                                                                                                             >  2:444518/1‑141 (MQ=255)
                                      ccAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGAt                                                                                                        <  1:1529699/141‑1 (MQ=255)
                                      ccAATTTGTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGAt                                                                                                        <  2:1640201/141‑1 (MQ=255)
                                       cAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATa                                                                                                       >  1:2295510/1‑141 (MQ=255)
                                          ttttttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCAACAGCGTTCATATAATTTATTAGATACGa                                                                                                    >  2:1188866/1‑141 (MQ=255)
                                           tttttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGAt                                                                                                   >  1:358969/1‑141 (MQ=255)
                                           tttttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATAAGaa                                                                                                   >  2:3736390/1‑140 (MQ=255)
                                           tttttAAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGGAACACGGTGTCTTGTATATATATTTGTGTGTCCTGTTTCGCCGCCTTTATATGCTACAGCGTTCATATAGTTTATTTGATAAGAt                                                                                                   >  2:536801/1‑141 (MQ=255)
                                              ttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGtt                                                                                                <  1:4283553/141‑1 (MQ=255)
                                                cAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGAAGCAAGTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGtttt                                                                                              >  1:4158294/1‑141 (MQ=255)
                                                  aaaaaTATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTCTGCCACAGCGTTCATATAATTAATTAGATACGATGtttttt                                                                                            >  1:365097/1‑141 (MQ=255)
                                                  aaaaaTATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGtttttt                                                                                            >  1:1831707/1‑141 (MQ=255)
                                                          gtagtaAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCt                                                                                    >  2:3192596/1‑141 (MQ=255)
                                                           tagtaAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATTATTTATTAGATCGGATGTTTTACCTGTTCCTa                                                                                   >  1:1165340/1‑141 (MQ=255)
                                                             gtaAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTAtt                                                                                 <  1:2222158/141‑1 (MQ=255)
                                                                    ttGGCAATAATCATTAATGCAGTAAAAATAAACAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCTACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCat                                                                          >  2:79165/1‑140 (MQ=255)
                                                                     tGGCAATAATCATTAATGCAGTAAAAATGATCAACCTTTTCGTGTATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGt                                                                         <  1:1877996/141‑1 (MQ=255)
                                                                     tGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGt                                                                         <  1:2253091/141‑1 (MQ=255)
                                                                       gCAATAATCATGAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTg                                                                       <  1:75903/141‑1 (MQ=255)
                                                                        cAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGa                                                                      >  1:3514764/1‑141 (MQ=255)
                                                                        cAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGa                                                                      <  2:1076204/141‑1 (MQ=255)
                                                                           taatCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGt                                                                   <  2:4385780/141‑1 (MQ=255)
                                                                           taatCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGt                                                                   <  2:2569418/141‑1 (MQ=255)
                                                                             atCATTAATGGTGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCATAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGttt                                                                 <  2:3337956/141‑1 (MQ=255)
                                                                             atCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGttt                                                                 <  1:1574725/141‑1 (MQ=255)
                                                                               taTTAATGCAGTAAAAATGATCTACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTttgt                                                               <  2:15378/140‑1 (MQ=255)
                                                                                 ttAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTttgttg                                                             >  1:1629494/1‑141 (MQ=255)
                                                                                         gTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCGGTTGAAGTTTGTTGAATTTGAg                                                     >  1:1429655/1‑141 (MQ=255)
                                                                                         gTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAg                                                     >  2:589466/1‑141 (MQ=255)
                                                                                           aaaaaTGATGAACATTTGCGTTGATATATATTTGTTTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGtt                                                   <  2:1731090/141‑1 (MQ=255)
                                                                                           aaaaaTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGtt                                                   <  2:2295510/141‑1 (MQ=255)
                                                                                           aaaaaTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGtt                                                   <  2:358969/141‑1 (MQ=255)
                                                                                            aaaaTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGttt                                                  >  1:3285883/1‑141 (MQ=255)
                                                                                            aaaaTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTACTGTTGCTATTTTTTCAGTTGCAGTTTGTTGAATTTGAGttt                                                  >  2:1323604/1‑141 (MQ=255)
                                                                                             aaaTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGtttt                                                 >  2:4158416/1‑140 (MQ=255)
                                                                                             aaaTGATCAACATTCGCTTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTGTGTTGCTATTGTTTTCTTTTATGTTTTTTGAGTTTGTGTTTc                                                 >  2:2731268/1‑141 (MQ=255)
                                                                                              aaTGATCAACCTTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCa                                                <  1:2853424/141‑1 (MQ=255)
                                                                                               aTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGAGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGACTTTGAGTTTCat                                               >  2:3077836/1‑140 (MQ=255)
                                                                                                 taTCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTGTATGGTAGGTGGTTCATCTAATTTTTTATATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAAtt                                             <  2:4289128/140‑1 (MQ=255)
                                                                                                 gATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGCGTTTCAAtt                                             >  2:435818/1‑141 (MQ=255)
                                                                                                 gATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAAtt                                             <  1:1363615/141‑1 (MQ=255)
                                                                                                  aTCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAAttt                                            >  1:882609/1‑141 (MQ=255)
                                                                                                  aTCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAAttt                                            >  1:3791986/1‑141 (MQ=255)
                                                                                                   tCAACATTTGCGTTGATTTATATTTGTGTGTCATGTTTCGCCGCCTTTCTTTGCTAGATGGTTATTATCATTTAGTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTtg                                           <  2:1111705/141‑1 (MQ=255)
                                                                                                      aCATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTCTTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGc                                        >  1:1324709/1‑141 (MQ=255)
                                                                                                        ttttGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCat                                      <  2:220411/140‑1 (MQ=255)
                                                                                                        aTTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCat                                      <  2:3964569/141‑1 (MQ=255)
                                                                                                               ttGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGc                               >  1:2976605/1‑141 (MQ=255)
                                                                                                                tGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTTAAGTTTTTTGAATGTGAGTTTCAATTTGTGCATAATATGCt                              <  1:3043311/141‑1 (MQ=255)
                                                                                                                tGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCt                              <  1:69824/141‑1 (MQ=255)
                                                                                                                 gATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCtt                             >  2:2357740/1‑141 (MQ=255)
                                                                                                                 gATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCtt                             >  1:2142320/1‑141 (MQ=255)
                                                                                                                   tatatatTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCa                           <  2:4024540/141‑1 (MQ=255)
                                                                                                                      atatTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACt                        <  1:3397048/141‑1 (MQ=255)
                                                                                                                        atTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACttt                      <  2:1443140/141‑1 (MQ=255)
                                                                                                                          ttGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTAt                    <  2:1502195/141‑1 (MQ=255)
                                                                                                                            gtgtgtCATGTCTCGCCGCCTTTCGATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTc                  <  1:2037101/141‑1 (MQ=255)
                                                                                                                              gtgtCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTcca                >  2:4222647/1‑139 (MQ=255)
                                                                                                                                 tCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTCTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAActc             <  1:1688044/141‑1 (MQ=255)
                                                                                                                                  cATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATCTAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGATTTTCATTTTGTGCATAATATGCTTCTATTTTATTCAActct            >  2:4086160/1‑141 (MQ=255)
                                                                                                                                       ttCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGAttt       <  2:1291024/141‑1 (MQ=255)
                                                                                                                                        tCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTTTTTTTTTTTTTTTTCAGTTGAAGTTTGTTGAATTTTTTTTTCTTTTTGTGCTTAATATGCTTCATCTTTATTCTTCTCTGttttt      >  2:2473837/1‑140 (MQ=255)
                                                                                                                                        tCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTTAATTTGAGTTTCAATTTGTGCATAATATGCTTTAACTTTATTCATCTCTGATTTa      >  2:3535944/1‑141 (MQ=255)
                                                                                                                                        tCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGATTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTa      >  1:3212364/1‑141 (MQ=255)
                                                                                                                                        tCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTa      >  1:1271614/1‑141 (MQ=255)
                                                                                                                                        tCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTa      >  2:880572/1‑141 (MQ=255)
                                                                                                                                        tCGCAGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTTAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCACTTTTATTCAACTCTAATTTa      >  2:1834833/1‑141 (MQ=255)
                                                                                                                                          gccgccTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCTACTTTATTCAACTCTGATTTAat    >  2:3449039/1‑141 (MQ=255)
                                                                                                                                          gccgccTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTGTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTAat    >  1:2569913/1‑141 (MQ=255)
                                                                                                                                            cgccTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTAatat  >  1:3916684/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAATATGCTTCAACTTTATTCAACTCTGATTTAATAT  >  USA300TCH1516_ALE/2028512‑2028792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: