BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,124,529	C→A	8.0%	D219E (GAC→GAA)	lexA_2 →	LexA repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,124,529	0	C	A	8.0%	999.1 / 17.4	355	D219E (GAC→GAA)	lexA_2	LexA repressor
Reads supporting (aligned to +/- strand): ref base C (196/121); new base A (2/36); total (198/157)
Fisher's exact test for biased strand distribution p-value = 3.95e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

GAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGA  >  USA300TCH1516_ALE/2124389‑2124669

   aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAt                                                                                                                                           >  2:2428705/1‑141 (MQ=255)

   aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAt                                                                                                                                           >  2:1355261/1‑141 (MQ=255)

         tttGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGATGTAGAAGACTGGTATAAGAAAGTCTTTGTTGAAGATGATAGAATGACTCTAGTTTAAATAAATAAAGATTACGAAGATATacac                                                                                                                                     >  2:532268/1‑141 (MQ=255)

         tttGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTacac                                                                                                                                     <  1:2977993/141‑1 (MQ=255)

             tgGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGAGTACGACGATCTACACtttt                                                                                                                                 <  1:1253504/141‑1 (MQ=255)

                agaAAACTCACAATATAAAGAATGGACAAATTGGTATATTCCTCACTGAATAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTtata                                                                                                                              <  1:1435417/141‑1 (MQ=255)

                        cacaATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGaa                                                                                                                      >  2:1564359/1‑141 (MQ=255)

                             tataACGAATGCACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTCTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAgtgt                                                                                                                 <  1:63497/141‑1 (MQ=255)

                              gtaAAGAATGGAGGAATTGGGATATTCAGCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATTATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGtgtg                                                                                                                <  2:1836877/140‑1 (MQ=255)

                                   gAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTATGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTAACTAAATAAAGATTCAGTCCAACGACACTTTTATAGAAATGAAAGTGTGAGGtt                                                                                                           <  2:3605638/141‑1 (MQ=255)

                                    aaTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTa                                                                                                          <  1:3316963/141‑1 (MQ=255)

                                      tGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAAt                                                                                                        >  1:1616036/1‑141 (MQ=255)

                                        gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                                      >  1:260445/1‑141 (MQ=255)

                                        gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                                      >  1:3797392/1‑141 (MQ=255)

                                        gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                                      >  1:4215537/1‑141 (MQ=255)

                                        gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                                      <  1:1759111/141‑1 (MQ=255)

                                        gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTAACTAAATAAAGATTACGGCGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                                      <  2:220065/141‑1 (MQ=255)

                                         aCAAATTGGTATATTCATGATTGAAGAAGATGTGTACGTTAATAAAGTTTTTGTTGAAGACGACAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                                     <  2:3820201/141‑1 (MQ=255)

                                         aCAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTTATTTTATTTTAATGAAACAAAGATTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                                     <  2:1166979/141‑1 (MQ=255)

                                         aCAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                                     >  1:3631170/1‑141 (MQ=255)

                                         aCAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                                     >  2:3734226/1‑141 (MQ=255)

                                         aCAAATTGGGATAGTCATCATTGAAGAAGAAGCGGACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                                     <  1:325777/141‑1 (MQ=255)

                                           aaaTTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGaa                                                                                                   <  1:1895194/141‑1 (MQ=255)

                                            aaTTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGaaa                                                                                                  >  1:2485075/1‑141 (MQ=255)

                                            aaTTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGACGATAGCTTGACTATAGTATCACTAAATAAAGATTCAGACGATCTACACTCTTACAGACATGAAAGTGTGAGGCTACTTGCaaa                                                                                                  >  2:743147/1‑141 (MQ=255)

                                              ttGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAg                                                                                                >  1:3016327/1‑141 (MQ=255)

                                              tgtgTATATTCATGAGTGAAGAAGAAGGTTACTTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGAGACAACGACTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAg                                                                                                <  2:2695026/138‑1 (MQ=255)

                                                ggTATATTCATCATTGAAGAAGAAGCGTACGTTAAGCAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGtt                                                                                              >  2:4313178/1‑141 (MQ=255)

                                                ggTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGCCTCTAGTTTCACTAAATCAAGATGATGACGATCTACACTTTTATAGAAAAGAAAGTGTGAGGTTAATTGCAAAAGtt                                                                                              >  2:2001137/1‑141 (MQ=255)

                                                     atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGCTTGACGCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAtttt                                                                                         <  1:389758/141‑1 (MQ=255)

                                                     atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAtttt                                                                                         <  1:1329615/141‑1 (MQ=255)

                                                     atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAtttt                                                                                         <  1:121151/141‑1 (MQ=255)

                                                     atTCATCAGTGAAGAATAAGCGTACGTTAAGAAATGCTTTGTTGAAGATTATAGATTTATTCTTGTTTCACTAAATAAAGATTACGGCGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAtttt                                                                                         <  2:6337/141‑1 (MQ=255)

                                                     atTCATCACTTGAGAAGAAGCGTATGTTAACAAAGTCTTTATTGAAGATGATAGATTGACTCTATTTTAATGAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAtttt                                                                                         <  2:3271053/141‑1 (MQ=255)

                                                       tcatcaTTGAAAAACAAGCGTACGTTCAGAAAGTCTTTGTCGAAGACGATAGATCGCCTCTAGTTTCACTAAATAAAGATTACCACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTtat                                                                                       <  1:564107/141‑1 (MQ=255)

                                                       taataatTGAAGAGGAAGCGTAAGTTAAGAAAATCTTTGTTTAAGATGATAGATTGTCTCTTTTTTAACGAAATAAAGATTACGAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTtat                                                                                       <  2:4192069/136‑1 (MQ=255)

                                                         atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATaa                                                                                     <  1:3178795/141‑1 (MQ=255)

                                                          tcatTGAAGAAGAAGCTTTCTTTAAGAAAGTCATTGTGGAAGATGATAGATTGTTTTTTTTTTAATGATACAGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATaaa                                                                                    <  2:2647217/141‑1 (MQ=255)

                                                             ttGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAgg                                                                                 <  2:692048/141‑1 (MQ=255)

                                                                aagaagaagCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGc                                                                              <  1:1014532/141‑1 (MQ=255)

                                                                aagaagaagCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGc                                                                              >  2:1419059/1‑141 (MQ=255)

                                                                 agaagaagCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCa                                                                             <  1:4207694/141‑1 (MQ=255)

                                                                 agaagaagCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTGTCACTAAATAAAGATTACGACGATCTCCACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTCTACAAGAAGCa                                                                             >  2:2967937/1‑141 (MQ=255)

                                                                 agaagaagCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGCTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTCGAAAAGTTATTTTATAAAATGAGCa                                                                             >  2:1679397/1‑141 (MQ=255)

                                                                   aagaagCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                           >  1:504505/1‑141 (MQ=255)

                                                                    agaagCGTACGATAAGAAAGTATTGGTAGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACAGTTTTATAGAAATGAAAGTGTGAGGCTAATTGGACAAGTTATTTTATAAAAGGCACACtt                                                                          >  2:2784874/1‑141 (MQ=255)

                                                                        gCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCaa                                                                      >  2:1503091/1‑141 (MQ=255)

                                                                         cGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTACTTTATAAAAGGAGCACTTGCaaa                                                                     >  2:46470/1‑141 (MQ=255)

                                                                           tACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATg                                                                   >  1:538966/1‑141 (MQ=255)

                                                                             cGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTGAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCACATGaa                                                                 >  2:3991383/1‑141 (MQ=255)

                                                                               ttAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaaa                                                               <  2:1079546/141‑1 (MQ=255)

                                                                                tAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAGTCAAAGTGTGATGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaaaa                                                              <  1:1695661/141‑1 (MQ=255)

                                                                                tAAGAAAATCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTTAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaaaa                                                              <  1:463973/141‑1 (MQ=255)

                                                                                  aGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTGCACTAAATAAAGGTTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAat                                                            <  1:3737789/141‑1 (MQ=255)

                                                                                  aGAAAGTCTTTGTTGAAGACGATAAATTGACTCTAGGTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAat                                                            <  1:1495971/141‑1 (MQ=255)

                                                                                       gTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATCACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGat                                                       <  1:3061912/141‑1 (MQ=255)

                                                                                          tttGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATAtt                                                    >  2:376244/1‑141 (MQ=255)

                                                                                           ttgttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGACAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAACATATGATAttt                                                   >  2:257041/1‑140 (MQ=255)

                                                                                             gttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAACATATAATATTGCa                                                 >  2:206033/1‑141 (MQ=255)

                                                                                                gAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACCCATAGAGAGTAATGAAAATGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTc                                              >  2:3342200/1‑141 (MQ=255)

                                                                                                gAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTc                                              <  1:1809842/141‑1 (MQ=255)

                                                                                                 aaGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCt                                             <  1:771784/141‑1 (MQ=255)

                                                                                                  aGATGATAGATTGACTTTTGTTTAACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCtt                                            <  2:1225372/141‑1 (MQ=255)

                                                                                                   gatgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCGGTCTTa                                           >  1:572567/1‑141 (MQ=255)

                                                                                                   gatgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTa                                           >  2:348036/1‑141 (MQ=255)

                                                                                                    atgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAg                                          <  2:3972508/141‑1 (MQ=255)

                                                                                                    atgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAg                                          <  1:137831/141‑1 (MQ=255)

                                                                                                    atgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAg                                          <  1:2920793/141‑1 (MQ=255)

                                                                                                     tgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAACTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGCTATTTCAGTCTTaaa                                         >  2:3134291/1‑139 (MQ=255)

                                                                                                      gatAGATTTACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                        <  1:1090388/141‑1 (MQ=255)

                                                                                                       atAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACt                                       >  2:1872475/1‑141 (MQ=255)

                                                                                                        tAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACtt                                      >  1:3958181/1‑141 (MQ=255)

                                                                                                         aaaTTGACTCTAGTTTAACTAAATAAAGATTACGGCGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                     <  2:3425848/139‑1 (MQ=255)

                                                                                                         aGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                     >  2:906771/1‑141 (MQ=255)

                                                                                                            ttGACTCTTGTTTAAATAAATAAAGATTACGAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGaa                                  <  2:2507478/141‑1 (MQ=255)

                                                                                                            ttGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGaa                                  <  1:3426876/141‑1 (MQ=255)

                                                                                                            ttGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGaa                                  <  1:2285320/141‑1 (MQ=255)

                                                                                                             tGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGaaa                                 <  1:685425/141‑1 (MQ=255)

                                                                                                               aCTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACt                               >  1:2212180/1‑141 (MQ=255)

                                                                                                                 tctTGTTTCACTAAATAAAGATTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTat                             <  2:213815/141‑1 (MQ=255)

                                                                                                                 tctAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAATTTGAAACTat                             >  2:3367412/1‑141 (MQ=255)

                                                                                                                  ctAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatg                            <  1:2717751/141‑1 (MQ=255)

                                                                                                                   tAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatga                           >  1:2345105/1‑141 (MQ=255)

                                                                                                                    aGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatgaa                          >  1:3128164/1‑141 (MQ=255)

                                                                                                                    aGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatgaa                          <  2:1315671/141‑1 (MQ=255)

                                                                                                                     gTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatgaat                         >  1:2560258/1‑141 (MQ=255)

                                                                                                                      tttCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatgaatg                        >  2:2867874/1‑141 (MQ=255)

                                                                                                                         cACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAAc                     >  2:1001746/1‑141 (MQ=255)

                                                                                                                         cACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTATTAGACTTTGAAACTATGAATGAAc                     >  2:3109982/1‑141 (MQ=255)

                                                                                                                         aatgaaatgaaGATCACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAAc                     <  2:1817391/137‑1 (MQ=255)

                                                                                                                             aaataaaGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACatat                 <  1:2428705/141‑1 (MQ=255)

                                                                                                                             aaataaaGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACatat                 <  1:3566542/141‑1 (MQ=255)

                                                                                                                              aataaaGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATg                >  1:4071113/1‑141 (MQ=255)

                                                                                                                               ataaaGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGa               >  1:1569638/1‑141 (MQ=255)

                                                                                                                                   aGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAg           >  2:847906/1‑141 (MQ=255)

                                                                                                                                        acgacgATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCtt      >  1:3601120/1‑141 (MQ=255)

                                                                                                                                        acgacgATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCtt      >  2:598269/1‑141 (MQ=255)

                                                                                                                                        acgacgATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCtt      <  1:906771/141‑1 (MQ=255)

                                                                                                                                          gacgaTCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGACCATATTAACAGCCCTTGc    <  2:1616036/141‑1 (MQ=255)

                                                                                                                                           acgaTTTACACTTTTATAGAAATAAAAGGGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCg   <  1:2025703/141‑1 (MQ=255)

GAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGA  >  USA300TCH1516_ALE/2124389‑2124669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`