BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,981	A→G	6.5%	D735D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,981	0	A	G	6.5%	976.8 / 11.7	420	D735D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (194/180); new base G (9/23); total (211/209)
Fisher's exact test for biased strand distribution p-value = 1.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 473 total aligned reads displayed.

GAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTC  >  USA300TCH1516_ALE/2774841‑2775121

gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                                              <  1:4017446/141‑1 (MQ=255)

 aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                             <  1:2596948/141‑1 (MQ=255)

  atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTTTCTGAGTCTGAGTCGCTatctgagtc                                                                                                                                            >  2:4150853/1‑141 (MQ=17)

  atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                            <  1:52622/141‑1 (MQ=255)

  atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                            <  1:2474308/141‑1 (MQ=255)

   tcACTGTCTGAATCCGAATCGATCTCTGGATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGTATCTTATTCGCTGTCTGATTCCGAATCGCTATCTGATTCTGAGTCGCTGTCTGTGTCTGATTCGCTatctgattct                                                                                                                                           >  2:2211000/1‑141 (MQ=17)

     cgtGTCTGAATCCGACTCTCCATCTGAATAGGACTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                                                                                                         <  2:3698391/139‑1 (MQ=14)

           tGAATCCGAATCGCTATCTGAATCTGAATCGCTATACGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCt                                                                                                                                   >  2:4305422/1‑141 (MQ=255)

                ccGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCCCTGtctg                                                                                                                              >  2:2812375/1‑141 (MQ=255)

                 cGAATCGGTGTCTTATTCTGACTCGTTATCTGAGTCTTAGTCTCTGGCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctga                                                                                                                             <  2:1105011/141‑1 (MQ=11)

                    aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGTGTCGCTAtctgagac                                                                                                                          >  2:847396/1‑139 (MQ=255)

                    aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                                                                          <  1:663286/141‑1 (MQ=255)

                      cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctg                                                                                                                        >  1:1018185/1‑141 (MQ=255)

                      cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctg                                                                                                                        <  1:4255751/141‑1 (MQ=255)

                         tATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTGTGAGGCTGAATCGCTATCGGAATCGGAGTCGCTAtctgagtctgagt                                                                                                                     >  1:216422/1‑141 (MQ=14)

                          ctcctaatctgcttcGCCAGCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCTCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                                    <  1:1110170/136‑1 (MQ=14)

                          atctgaatctgaATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                                    >  1:935632/1‑141 (MQ=255)

                          atctgaatctgaATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                                    <  1:603589/141‑1 (MQ=255)

                             cgaatctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgct                                                                                                                 <  1:1635056/140‑1 (MQ=255)

                                   tgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgcgtgtgatgcgctgtcgga                                                                                                           >  2:3533987/1‑141 (MQ=14)

                                   tgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctga                                                                                                           <  1:3750556/141‑1 (MQ=255)

                                   tgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGACTCGCTGTCTGAATCTGAATCACTATCTGAATCTGCGTCGCTAtctgagtctaattcactatctga                                                                                                           >  2:3614228/1‑141 (MQ=14)

                                    gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtgtgag                                                                                                          >  1:3103270/1‑141 (MQ=255)

                                    gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgag                                                                                                          >  1:3217382/1‑141 (MQ=255)

                                      ttcGCTATCTGAGTCGGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCCGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                                        <  1:2933330/140‑1 (MQ=11)

                                       tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtct                                                                                                       >  1:2539059/1‑141 (MQ=255)

                                        cGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctg                                                                                                      <  1:1904101/141‑1 (MQ=255)

                                         gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgccgtctgagtctga                                                                                                     >  1:3605352/1‑141 (MQ=255)

                                         ccTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctga                                                                                                     <  1:2856142/140‑1 (MQ=255)

                                          cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                                                                                    >  1:2365937/1‑141 (MQ=255)

                                           tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                                                                   <  2:3289023/141‑1 (MQ=255)

                                           tATCCGAGTCTGAGTCGCTGTCTGAATCTGAAGCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                                                                   <  1:1313800/141‑1 (MQ=255)

                                             tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgcgtctgagtcg                                                                                                 >  1:315391/1‑141 (MQ=255)

                                             tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcg                                                                                                 >  1:3793088/1‑141 (MQ=255)

                                              ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgc                                                                                                >  1:4397709/1‑141 (MQ=255)

                                               cGAGGCTGAGTCGCTGTCTGATTCTGAGTCTCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                                                                               <  2:1022281/141‑1 (MQ=14)

                                                    cTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGACCCGCTACCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGagtctgagtcgctgtctgagtctgagtcgctgtctg                                                                                          <  1:148691/141‑1 (MQ=255)

                                                      gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTCTCTGAATCTGAGTCGAACCCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAgtctgagtcgctgtctgagtctgagtcgctgtctgaa                                                                                        >  2:3844476/1‑140 (MQ=14)

                                                       aGTCGCTCTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCCATCTGAATCTGAGTCGCTATCTGAGTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                                                       <  1:3483164/141‑1 (MQ=255)

                                                        gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                                      <  1:1988284/141‑1 (MQ=255)

                                                          ggCTTTCTGATTCTGTAGCGCTGTCTGAGTCTGTTTCGCTATCTGTGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGagtcgctgtctgagtctgagtcgctgtctgagtctg                                                                                    <  2:96397/140‑1 (MQ=14)

                                                           gCTCTCTGCATGTTAATCGTGGTCTGAGTCTGAGTCGTTCGCTTAATCTGAGTCGCTGTCTGAGGCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAgtcgctgtctgagtctgagtcgctgtccgagtctga                                                                                   <  2:4052029/141‑1 (MQ=14)

                                                            cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTCTCTGAATCTGAGTCTCTATCTGAATCTGAGTCTCTATCTGACTCTTCGTCGCTCtctgaatctgaa                                                                                  >  2:1445585/1‑141 (MQ=11)

                                                              gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATAGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                >  1:3303926/1‑141 (MQ=255)

                                                               tctgactctgacTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                               <  1:982997/141‑1 (MQ=255)

                                                                 tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                             >  1:896250/1‑141 (MQ=255)

                                                                 tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                             >  1:771260/1‑141 (MQ=255)

                                                                   aatctgaatcGCTGTCTGAGTCCGAATCGCTATATGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTTGCTATCTGAGTCTGAGTTGCTGGCTCAATCTGAGTCGCTATCTGAGTCTGAATAGCtct                                                                           >  1:7394/1‑139 (MQ=11)

                                                                    ctctgaatcGCTGTCTGAGTCCGGAGCGTTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGAATCTGAGTCGCTATCGGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                          <  2:1353998/140‑1 (MQ=17)

                                                                    atctgtgtcGCTGTGTGAGTGCGAATCGCTATATGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATcacttgc                                                                          <  2:1862633/141‑7 (MQ=17)

                                                                    atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTgtc                                                                          >  1:3561545/1‑141 (MQ=255)

                                                                    atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCCatc                                                                          >  1:3378960/1‑141 (MQ=255)

                                                                     tctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTGCGCTATTCAAGCTTAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTatct                                                                         >  1:1027597/1‑141 (MQ=14)

                                                                        ttttcGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                      <  2:2337177/138‑1 (MQ=14)

                                                                             gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGCGTCACTGTCTGAGTCTGACTCGCTgtcggcatcgga                                                                 >  2:2536531/1‑141 (MQ=14)

                                                                               tGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCAGAGTCGCTGTCTGTGTCCGAATCGCTatctgaatatgaaa                                                               >  2:1205690/1‑139 (MQ=14)

                                                                                gtctgagtctgaGTCGCTTTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                              <  2:3476312/141‑1 (MQ=14)

                                                                                gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGACTCGCTCTCTGAGTCTGAGACCCTCCCTGACTCCGAATCCCTatctgaatccgaagc                                                              >  2:1773499/1‑138 (MQ=14)

                                                                                 tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCTGCGTCg                                                             >  1:3891059/1‑141 (MQ=255)

                                                                                  cggAGTCTGAGTCGTTATCTGAATCTGAGTCGCTGTCTGAGTCGGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                                            <  2:69958/139‑1 (MQ=255)

                                                                                  cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                                            >  1:3037540/1‑141 (MQ=255)

                                                                                   tGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGCGTCGCt                                                           >  1:2705307/1‑141 (MQ=255)

                                                                                   tGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGGCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCGGAATCCAGATCTGAATCTGAGTCACt                                                           >  2:1529629/1‑141 (MQ=11)

                                                                                      gTCTGAGTCGCTATCTGAATCTGAGTCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGATTCTCTTTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtc                                                        <  2:531515/141‑1 (MQ=14)

                                                                                       tCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGGCTGAATCGCTATCTGAATCTGAGTCGCTgtct                                                       >  1:3425193/1‑141 (MQ=255)

                                                                                        cggAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                      <  2:2395526/139‑1 (MQ=255)

                                                                                        ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                      <  1:1834581/141‑1 (MQ=255)

                                                                                           aaTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                   <  1:1253959/141‑1 (MQ=255)

                                                                                           aaTCGCTATCTGAATCGGAGTCGCTGGCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                   <  1:2142941/141‑1 (MQ=255)

                                                                                            aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                  >  1:4324796/1‑141 (MQ=255)

                                                                                             tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtct                                                 <  1:3701158/141‑1 (MQ=255)

                                                                                              cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                <  1:2433609/141‑1 (MQ=255)

                                                                                                cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCAGTCTGAGTCTCAATCGCTACCTGAGTCTGAATCGCAatctgagtctgaa                                              >  2:3225066/1‑140 (MQ=11)

                                                                                                  ctctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCtgagtctgagtc                                            <  2:4352278/140‑1 (MQ=255)

                                                                                                   tctgatgctgaGTCGCTTTCCGAGTCTGAATCGCTGTCTGAATCTGACTCGCTATCTGAGTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                           <  2:2984204/141‑1 (MQ=14)

                                                                                                   tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTATGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCtgaaacggagtca                                           >  2:3938505/1‑140 (MQ=14)

                                                                                                        tgctgcGTCGCTGTCGGAGTCTGTTTCGCTTTCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                      <  2:2071166/139‑1 (MQ=14)

                                                                                                          ctgaGTCGCTGTCTGCGTCTGAATCGCTGTCTGAATCTGACGCGCTATCTGAGTCTGAGTCGCTTTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                                    <  2:771260/141‑1 (MQ=17)

                                                                                                          ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCGGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                                    <  1:289890/141‑1 (MQ=255)

                                                                                                           tgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCCCTATCTGATGCTGACTCGCTATCAGTGtctgagccgctctgacg                                   >  2:2607866/1‑135 (MQ=11)

                                                                                                            gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaa                                  >  1:4185932/1‑141 (MQ=255)

                                                                                                             aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                 >  1:2648870/1‑141 (MQ=255)

                                                                                                              gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCTGACTCGCTGTCTGAGtctgagtcgctgtctacatc                                >  2:3354856/1‑136 (MQ=255)

                                                                                                              gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatc                                <  1:1600113/141‑1 (MQ=255)

                                                                                                                ggCTATCTTAGTCTGGGTGTCTGTCTGATTCTGCATCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                              <  2:913287/140‑1 (MQ=11)

                                                                                                                 gCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                             >  1:1161341/1‑141 (MQ=255)

                                                                                                                     tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                         <  1:2971236/141‑1 (MQ=255)

                                                                                                                     tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                         >  1:1553006/1‑141 (MQ=255)

                                                                                                                      ctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgc                        <  1:4280850/141‑1 (MQ=255)

                                                                                                                      ctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgc                        >  1:847091/1‑141 (MQ=255)

                                                                                                                         ggtctgaGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgt                     <  2:1633880/140‑1 (MQ=255)

                                                                                                                           tctgaGTCGCTGTCTGATTCTGAGTCGCTTTCTGTGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                   <  2:191206/141‑1 (MQ=11)

                                                                                                                            ctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg                  <  1:1430238/141‑1 (MQ=255)

                                                                                                                            ctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg                  <  1:3657739/141‑1 (MQ=255)

                                                                                                                            ctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg                  <  1:2998064/141‑1 (MQ=255)

                                                                                                                                aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCtgagtcgctgtctgaatctgagtcgctgtctgaatc              <  1:226223/141‑1 (MQ=255)

                                                                                                                                 tCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTgagtcgctgtctgaatctgagtcgctgtctgaatct             >  1:2773455/1‑141 (MQ=255)

                                                                                                                                   gCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                                  >  1:1774103/1‑38 (MQ=255)

                                                                                                                                      atctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTCTCTGACTCTGAAGCGCTGTCTGAGTCTGAGTCCCTCTCAGACACTGAATCACTAtctgaatctgaaac        >  2:3052653/1‑139 (MQ=14)

                                                                                                                                        ctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGc      <  1:542593/141‑1 (MQ=255)

GAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTC  >  USA300TCH1516_ALE/2774841‑2775121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`