BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,775,287	A→G	5.7%	D633D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,775,287	0	A	G	5.7%	637.1 / 4.7	264	D633D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (138/99); new base G (5/13); total (148/116)
Fisher's exact test for biased strand distribution p-value = 1.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 484 total aligned reads displayed.

GAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTGAGTCGCTATC  >  USA300TCH1516_ALE/2775147‑2775427

gaatcgctatctgagtctgaatcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatcttaa                                                                                                                                              >  1:1179220/1‑141 (MQ=255)

 aatcgctggctgagtctgaatcACTGTCTGAGTCTGAGTCACTATCTGACGCTGACTCACTTTCTCACTGTGCGTCGCTATCTGATTCTGAGTCGCTCTCTGAATATGAATCGCTGTCTGAGTCCgaatcgctatctgaat                                                                                                                                             <  2:3771221/141‑1 (MQ=14)

   tcgctatctgagtctgaatcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgcatggctatctgaatct                                                                                                                                           >  1:2126491/1‑141 (MQ=255)

   tcgctatctgagtctgaatcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGACTCGCTGTCTGATTCTGTATCACGATCTAAATCCgaatacctatctggatat                                                                                                                                           >  2:1652483/1‑139 (MQ=18)

      ctatctgagtctgaatcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTAATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCTgaatcgctatctgaatctgaa                                                                                                                                        >  1:4285261/1‑141 (MQ=255)

         tctgagtctgaatcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgattctgaatcg                                                                                                                                     >  2:196807/1‑141 (MQ=255)

         tctgagtctgaatcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcg                                                                                                                                     >  1:3558618/1‑141 (MQ=255)

          ctgagtctgcatcACTGTCTGAGTCCGAGTCAATGTACCAATCAGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgc                                                                                                                                    <  1:143104/141‑1 (MQ=18)

                   aatcTCTTTCTCCGTCCGAGACTCTGTCTGCATCTGACACTCTATCTGTATCTGAGTCGCTTTCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaat                                                                                                                           <  2:4186487/141‑1 (MQ=17)

                    atcACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatc                                                                                                                          >  2:3353430/1‑141 (MQ=255)

                      cgcTGTCTGAGTCCGAGTCGCTGTCTGAATCTGAGTCTCTATCTGATTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTgaatcgctatctgaatctgaatcgctatctgaatctg                                                                                                                        <  2:1436389/139‑1 (MQ=11)

                      cACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgactcactagctgaatctgaatcgctatctgaatctg                                                                                                                        >  2:712087/1‑141 (MQ=255)

                      cACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgcatctgaatcgctatctgattctg                                                                                                                        >  2:3524345/1‑141 (MQ=255)

                       aCTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGACTCACTGTCTGAATCCgactcgctctctgaatctgaatcgctatctggctctgg                                                                                                                       >  2:2521916/1‑140 (MQ=18)

                       aCTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctga                                                                                                                       <  1:1457645/141‑1 (MQ=255)

                        cTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaa                                                                                                                      >  1:2971713/1‑141 (MQ=255)

                         tGTCGGAGTCTGAGTTTCTGTCTGATTCGGCGTCACTAGCTGATTCTGAGTCGCTCTCTGATTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaat                                                                                                                     <  2:2958553/141‑1 (MQ=14)

                           tgaggttCCTAGTCTCTTTGTGAATCCGACTCGCTATGTGAATCTGACTCGCTATCTGAGTCTGAGTCGATGTCTGAATCTGACTCTCTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaatcg                                                                                                                   <  2:2867071/135‑1 (MQ=14)

                           tCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgactctgaatcg                                                                                                                   >  1:3837027/1‑141 (MQ=255)

                           tCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaatcg                                                                                                                   <  1:2502761/141‑1 (MQ=255)

                            cTGCGTCTGAGTCACTGTCTGAGTCTGACTCGCTCTCTGATTCTGAGTCGCTCTCTGATTCTGAGTCGCTGTCTGAATCTGAATCTCTGTCTGAATCTgaatcgctatctgaatctgaatcgctatctgaatctgaatcgc                                                                                                                  <  2:4133764/141‑1 (MQ=14)

                            cTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgagtcgctatccgactctgaatcgc                                                                                                                  >  1:3786554/1‑141 (MQ=255)

                             tGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGCATCACTGGCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaatcgct                                                                                                                 >  1:3691805/1‑141 (MQ=255)

                             tGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgagtcgctatctgaatctgaatcgct                                                                                                                 >  1:1250101/1‑141 (MQ=255)

                             tGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgagtcgct                                                                                                                 >  2:2341366/1‑141 (MQ=255)

                                ggccGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGCTTCTGTATTTCGGGCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaatcgctatc                                                                                                              <  1:3225066/139‑1 (MQ=18)

                                 tCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGACTCACTGTCTGACTCCgattcgctatctgtctctgtgtcgctctctgaatctgaatccatatct                                                                                                             >  2:3272126/1‑141 (MQ=17)

                                    gAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGACTCCgaatcgctatcaggttctgactctctatctgactctgactctgcatctgat                                                                                                          >  2:822733/1‑140 (MQ=14)

                                     tgTCACTGTCTGAATCTCGCTCTCTGTCTCTTTCTGAGTCGCTGTCTGTGTCTCAGTCGCTGTCTGAGTCTGAATCACTGTCTGAGTCCgaatcgctatctgaatctgaatcgctatctgaatctgaatcgctatctgatt                                                                                                         <  2:1480667/140‑3 (MQ=14)

                                       tCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatct                                                                                                       >  1:484003/1‑141 (MQ=255)

                                         aCTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAatcgctatctgaatctgaatcggtatctgaatctgaatcgctatctgaatttga                                                                                                     >  1:625475/1‑141 (MQ=255)

                                         aCTGTCTGAATCTGACTCACGATCTGATGCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCgaatcgcgatctggttctgagtctgtatctgcttctgagtcgctctctgacttctg                                                                                                     >  2:2711849/1‑135 (MQ=11)

                                          cTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGGATCTGAATCACTGTCTGAATCAGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAg                                                                                                    >  1:161847/1‑141 (MQ=255)

                                             tCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTCTCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCtgagtctgagtcc                                                                                                 >  2:3639234/1‑140 (MQ=255)

                                              cTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGc                                                                                                <  1:566444/141‑1 (MQ=255)

                                               tGAGTCTGACTCACTATCTGATTCTGAGTCGCTATGTGTTTTATAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCt                                                                                               <  2:3119847/141‑1 (MQ=18)

                                                   tCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCt                                                                                           >  1:3511200/1‑141 (MQ=255)

                                                   tCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCt                                                                                           >  1:2647264/1‑141 (MQ=255)

                                                   tCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGGCGCTGTCTGAATCTGAATCACTGTCTGAATCCAAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGCCGCTATCt                                                                                           >  1:2345769/1‑141 (MQ=255)

                                                      gACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAt                                                                                        >  1:3280587/1‑141 (MQ=255)

                                                       acgcACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAtt                                                                                       <  1:1751068/138‑1 (MQ=255)

                                                        cTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATGGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTc                                                                                      >  1:1569178/1‑141 (MQ=255)

                                                          cACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTg                                                                                    <  1:1395697/141‑1 (MQ=255)

                                                            ctgtctgagtctgagtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGaa                                                                                  <  1:1892660/138‑1 (MQ=255)

                                                            ctatctgattctgagtcgctatctgattctgagtcgCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAGTCTGAGTCGCTATCTGAATCTGATTCGCTATCTGATCCCGCGTCGCTCTCCGACTCCGaa                                                                                  >  2:649347/1‑141 (MQ=11)

                                                             tatgtgatcgtgactcgctgtctgattctgagtcgcTGTCTGAATCTGAATCACGGTCTCAATACGAAACGCTATCTGAATCTGAATCGCTATCTGAGTCTGGATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAAt                                                                                 <  2:3452122/141‑1 (MQ=14)

                                                                  gattctgagtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTa                                                                            >  1:3141635/1‑141 (MQ=255)

                                                                    ttctgagtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATAAGAGCCACGATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATc                                                                          >  1:3011853/1‑141 (MQ=17)

                                                                     tctgagtcgctatctgagtctgagtcgctGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCt                                                                         <  1:1017144/141‑1 (MQ=255)

                                                                     tctgagtcgctatctgagtctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCt                                                                         >  2:3193657/1‑141 (MQ=255)

                                                                      ctgagtcgctatgtgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTg                                                                        <  1:3965891/141‑1 (MQ=255)

                                                                       tgagtcgctatctgcttctgagtcgctGTCTGAATCTGAATCACTCTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTCTCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGa                                                                       <  1:735191/141‑1 (MQ=255)

                                                                       tgagtcgctatctgattctgagtcgctGTCTGAATCTGACTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGCATCTGAGTCGCTTTCTGACTCTGAGTCGCTTTCTCATTCTGAATCGCCATCTgg                                                                       >  2:3833267/1‑140 (MQ=14)

                                                                       tgagtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTCTCTGAATCTGAGTCGCTATCTGACTCCGAATCGCCATccgc                                                                       >  2:4297383/1‑138 (MQ=14)

                                                                        gagtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCTGAATCTGAATCAATCCCTCAATCTGAGTCGCTCTCTGATTCCGAATCCCGATCTGAt                                                                      >  2:4263993/1‑141 (MQ=14)

                                                                         agtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGAtt                                                                     <  1:2689689/141‑1 (MQ=255)

                                                                          gtcgctatctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTc                                                                    >  1:2795586/1‑141 (MQ=255)

                                                                                  ctgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGc                                                            <  1:1778459/141‑1 (MQ=255)

                                                                                   tgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTGAGTCGCTATCTGAATCTGACTCGCTATCTGACTCTGAGTCGCTCTCTGACTCTGAATCGCTATCTGTTTCTGATGCGCt                                                           >  2:1136748/1‑141 (MQ=14)

                                                                                   tgattctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCt                                                           <  1:335663/141‑1 (MQ=255)

                                                                                       tctgagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGCTTCTGAATCGCTATCTGATTCTGAGTCGCTGtct                                                       <  1:859373/141‑1 (MQ=255)

                                                                                          gagtcgctGTCTGACGCTGAATCGCTGTCTGGATCTGACTCGCTATCTCAATNTGACTCGCTCTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGtctgaa                                                    <  2:3987658/141‑1 (MQ=11)

                                                                                          gagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGtctgag                                                    >  1:3649785/1‑140 (MQ=255)

                                                                                          gagtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGtctgaa                                                    >  1:2555805/1‑141 (MQ=255)

                                                                                           agtcgctGTGTGACTCTGAATCTCTGTCTGTATCTGATTCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGtctgaat                                                   <  2:2119574/141‑1 (MQ=14)

                                                                                           agtcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGATATCTGAATCTGAATCGCTATCTGAATATGAGTCGCTATCTGGTTCTGAATCGCTATATGATTCTGCGTCGCGGtgggagt                                                   >  1:1855286/1‑135 (MQ=17)

                                                                                             tcgctGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGCTTCTGAGTCGCTGtctgaatct                                                 >  1:675119/1‑141 (MQ=255)

                                                                                                ctctctgcatctcacTCCCTATCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCGGTCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGtctgaatctgaa                                              <  2:2656003/138‑1 (MQ=14)

                                                                                                   tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCAGAATTGAAATCTGATTCTGAGTCGCTGTCTGAATCTGAATCa                                           >  1:3423768/1‑141 (MQ=255)

                                                                                                    ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCtgaatctgaatctc                                          >  1:2247299/1‑139 (MQ=255)

                                                                                                        atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTc                                      >  1:3464033/1‑141 (MQ=255)

                                                                                                          tttaatcAGTATCTTAATCCGAATCGCTATCTGAGTCTGAATCACTGTCTTAATCTGAATCACTATCTGAATCTTACTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTg                                    <  2:4388863/138‑1 (MQ=11)

                                                                                                          ctgattcGCTTTCTGAGTCTGAATCGCTATCTGAGTCTGACTCTCTATCTGAGTCTGAGTCGCTATCTGAAGCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTg                                    <  2:1583501/141‑1 (MQ=11)

                                                                                                          ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTCTCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTg                                    >  1:1930542/1‑141 (MQ=255)

                                                                                                          ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTg                                    <  1:977836/141‑1 (MQ=255)

                                                                                                              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATc                                <  1:4403252/141‑1 (MQ=255)

                                                                                                              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATc                                >  1:405744/1‑141 (MQ=255)

                                                                                                                  cTGTCTGAATCCgaatcgctatctgaatctgaatcgctatctgaatctga                                                                                                                       >  1:2883767/1‑50 (MQ=255)

                                                                                                                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGACTCTGAATCATTCTCTGAATCCGACTcc                         >  2:128461/1‑140 (MQ=255)

                                                                                                                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGc                        <  1:419997/141‑1 (MQ=255)

                                                                                                                        gAATCCGAATCGCTATGTGAATGTGAATTCCTATCTGAATCTGAATCGCTATCTGAATCTTATTCGCTATCTGATTCTTTATCGCTATCTAATTCTGATCCGCTTTCCGAATCTGAAGCACTGTCTCAATCCGAATctccc                      >  2:2210432/1‑137 (MQ=17)

                                                                                                                        gAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                      >  1:3936025/1‑141 (MQ=255)

                                                                                                                          aTCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATc                    <  1:1110029/141‑1 (MQ=255)

                                                                                                                          aTCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATc                    <  1:2290828/141‑1 (MQ=255)

                                                                                                                           tCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTCTCTGATTCTGAATCGCTATCTGATTCTGAGCCGCTGTCTGGATCTGAATCACCATCTGCATCCGAATCgacttct                   >  2:1073210/1‑135 (MQ=17)

                                                                                                                            ccGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTg                  >  1:2388707/1‑141 (MQ=255)

                                                                                                                            ccGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTg                  <  1:1516809/141‑1 (MQ=255)

                                                                                                                              gaatcgctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatctGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAt                <  1:81430/141‑1 (MQ=255)

                                                                                                                              gaatcgctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatctGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAt                <  1:909909/141‑1 (MQ=255)

                                                                                                                               agtcgctatctgaatctgagtcgctgtctgagtctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAtt               <  2:2476641/139‑1 (MQ=255)

                                                                                                                               actcgctatctgcatctgaatcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAtt               <  1:198039/139‑1 (MQ=255)

                                                                                                                               aatcgctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatctgAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAtt               <  1:4250162/141‑1 (MQ=255)

                                                                                                                               aagtgctgtctgaatctgaatcgtgctctgaatctgaatcgctgtctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGACTCGCTGTCTGAATCCGAATCGCTATCTGAtt               <  2:325235/137‑1 (MQ=14)

                                                                                                                               aagcgctatttgagtctgaatcgccatctgagtctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAtt               <  2:3691670/138‑1 (MQ=17)

                                                                                                                                atcgctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGACGCTGTCTGAATCTGAATCACTCTCTGAATCCGAATCGCTATCTgtctt              >  2:1857174/1‑137 (MQ=255)

                                                                                                                                    ctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTgcg          >  2:530821/1‑139 (MQ=255)

                                                                                                                                    ctatctgaatctgaatcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGATGTCTGAATCTGAATCCCTGTCTGAATCCGAATCGCTATCTGATTCTGAg          >  2:301300/1‑141 (MQ=255)

                                                                                                                                    ctatctgaatctctagcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTGAg          <  1:4132023/141‑1 (MQ=255)

                                                                                                                                        ctgaatctgaatcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTGAGTCGc      <  1:1318606/141‑1 (MQ=255)

                                                                                                                                            atctgaatcgctatctgaatctgaatcgctatctgaatctgaGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTGAGTCGCTATc  <  1:2316602/141‑1 (MQ=255)

GAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGATTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGATTCTGAGTCGCTATC  >  USA300TCH1516_ALE/2775147‑2775427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`