Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA93.3% 31.8 / ‑6.0 15A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/10);  minor base T (1/0);  total (5/10)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAT  >  USA300TCH1516_ALE/2279585‑2279822
                                                                                                                        |                                                                                                                     
gATTTTACTTCTGGTTTATTAGTTACATCTTTATTCATTGTCTGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCTCCt                                                                                                         <  2:72338/135‑1 (MQ=255)
 aTTCTACTTCTGGTTTACTTGTTACATCGTGATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGTTTGTGTTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg                                                                                                        <  1:204614/135‑1 (MQ=255)
        ttCTGGTTTACGAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                 <  2:91997/135‑1 (MQ=255)
         tCTGTTTTACTCGTTACATCGTTATCCATTATCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTGACCTGTTGCCGCa                                                                                                <  2:47551/135‑1 (MQ=255)
           tGGTTTACTAGATACATCGTTATACATTGTCGAACTGTTTGTTGATGCATCTAAAGTAGAATTGTTATGAGCCTGCGGTTTACAATTAGCATCATCAGTAGCTGATGTTATTGTGGTTTAACATGTTGTCGCATc                                                                                              >  2:181041/1‑135 (MQ=255)
                    ggTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATGGTTATTAGCTTACGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTAGCCGCATCACTattatt                                                                                     <  1:200749/134‑1 (MQ=255)
                      ttACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAAATATTGACGCATAACTCTTATTTg                                                                                   >  2:52872/1‑135 (MQ=255)
                                          ggACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTTCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCt                                                               >  1:200635/1‑135 (MQ=255)
                                                            ttgacacTAGAATTGTTATTAGCTTGCGGTTTATCAGTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCTGAGAAGCGTCTGCTTTGCCATTAGCTgtc                                             <  2:212723/132‑1 (MQ=255)
                                                                          gTTATTAGCTTGCGGTTTATCATTTGCATCATCAATTGCTAAAATTAAAATAGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                               >  1:29009/1‑135 (MQ=255)
                                                                                       tgTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTTTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTa                  <  1:215626/134‑1 (MQ=255)
                                                                                        gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTTCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAt                 <  1:166599/135‑1 (MQ=255)
                                                                                          ttATCATTTGCATCATCAGTTGTTCTCATTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGATAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt               <  1:37591/135‑1 (MQ=255)
                                                                                              gatgtGCATTATCAGTTGCTGATGTTACTGTTGTTTCACTTGTTGCCGCATCACTATTATTTGGTGTTGTCGGATAAGCGTTTCCTTTACTATTATCTTTTGTTTTAGATATGTTTGGTTGTCCAGTATTTTcgg           <  1:112213/131‑3 (MQ=255)
                                                                                                       catcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAt  >  2:154841/1‑135 (MQ=255)
                                                                                                                        |                                                                                                                     
GATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAT  >  USA300TCH1516_ALE/2279585‑2279822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑