Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,532,298	A→T	T15T (ACA→ACT)	femA_3 →	Aminoacyltransferase FemA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,532,298	0	A	T	100.0%	40.8 / NA	17	T15T (ACA→ACT)	femA_3	Aminoacyltransferase FemA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/9);  total (8/9)

TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTT  >  USA300TCH1516_ALE/2532167‑2532420                                                                                                                                    |                                                                                                                           tttGTAATCAGTTTTTGATAAAATAAAAGAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTactaca                                                                                                                         <  2:124276/135‑1 (MQ=255)      aaTCAGTTTTGGATAAAATAAAAAAATAGCAGTATTAATCGTTCTGGGAGGTTTTGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCa                                                                                                                    <  2:156649/135‑1 (MQ=255)          aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGATCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                >  1:133762/1‑135 (MQ=255)          aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAAATAAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                >  1:3924/1‑135 (MQ=255)           gTTTTTGATAAAATAAAAAAATATCATTATTAATAAATTCAAAAATTATAGATAAAATCAAAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                               >  1:176606/1‑135 (MQ=255)                      aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTAAGAGAGGGATGAGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTACATTacaca                                                                                                    >  1:73151/1‑135 (MQ=255)                          aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                >  2:128636/1‑135 (MQ=255)                            aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCTGATGAGTTCAATGCTTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                              <  1:107450/135‑1 (MQ=255)                                tAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCCGCTa                                                                                          >  2:194942/1‑135 (MQ=255)                                 aGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTAACATTACACACAAACacatat                                                                                         >  2:48609/1‑130 (MQ=255)                                                     aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTg                                                                     <  1:103372/135‑1 (MQ=255)                                                       aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAACGCGTTTACTACAAAGCATTTTTCATATTACACATTATCAGCTATTCATTACAATCATAGAGTTGAt                                                                   <  1:115407/135‑1 (MQ=255)                                                          ataGTTCAAGTCAGAGAGGGAGGGGATAAAATGTATTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACAAACAATCAGTTATTCAGTACAATCATAGAGTTGATTTa                                                                <  2:73151/135‑1 (MQ=255)                                                                               tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATTTGTTATTTTTTACAATTATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTa                                           <  1:45190/135‑1 (MQ=255)                                                                                            aCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTAATACAAAGCATTTTTCACATTAAACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                              >  1:147835/1‑135 (MQ=255)                                                                                                 gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGTGATGTGTATCTTGTAGGGGTTAAAGATGACAAt                         <  1:179316/135‑1 (MQ=255)                                                                                                                        cAATGTTTTTACTACAAATCATTTTTCACATTACACACAATCAGCTATTCATTACACTCCTAATGCTTTTTGAAAATGCGATGTTCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt  <  2:75226/135‑1 (MQ=255)                                                                                                                        cAATGCGTTTACTACAAAGCAATTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTTATTGCAGGATGCtt  <  2:94404/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                           TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTT  >  USA300TCH1516_ALE/2532167‑2532420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑