Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000731	81	N→G	intergenic (–/‑151)	– / → repA	–/replication protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	81	0	N	G	100.0%	182.9 / NA	55	intergenic (–/‑151)	–/repA	–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base G (33/22);  total (33/22)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATG  >  CP000731/1‑214                                                                                                                                           |                                                                                                                                      atatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGaa                                                                                                                                     <  2:254501‑M1/83‑1 (MQ=255)     ttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTg                                                                                                                                 >  2:105149‑M1/55‑141 (MQ=255)              gtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAATCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTttatttat                                                                                                                        >  1:181313‑M1/46‑141 (MQ=255)                ttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                      <  1:193402‑M1/98‑1 (MQ=255)                   taaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAt                                                                                                                   <  2:15505‑M1/101‑1 (MQ=255)                     aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                 >  1:6138‑M1/39‑141 (MQ=255)                      acccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTACTATAACTATAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAtttt                                                                                                                <  2:91100‑M1/104‑1 (MQ=255)                       cccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTc                                                                                                               >  1:137528‑M1/37‑141 (MQ=255)                        ccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAACCTGTTTCTTTATATAATTTTCg                                                                                                              <  1:85654‑M1/106‑1 (MQ=255)                         cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGa                                                                                                             >  1:114701‑M1/35‑141 (MQ=255)                          ttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGTGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGAt                                                                                                            >  2:62057‑M1/34‑141 (MQ=255)                              taaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACtt                                                                                                        <  2:250659‑M1/112‑1 (MQ=255)                                      tattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATAGTGAGAATTTTCGTTACTTGTATtata                                                                                                <  1:54089‑M1/120‑1 (MQ=255)                                       attttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtatat                                                                                               >  2:193402‑M1/21‑141 (MQ=255)                                            cccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGa                                                                                          >  1:104857‑M1/16‑141 (MQ=255)                                             ccttttataagtttATTTACAGTTCTACAATAAACGTAATATAACAAAAACCCCAACTACTGCTAATATTCTTTTTTTTGTAATAAAACGGTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaa                                                                                         <  2:148773‑M1/127‑1 (MQ=255)                                             ccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaa                                                                                         <  2:268488‑M1/127‑1 (MQ=255)                                                tttataagtttATTTAAAGTGCTATAATAAACTTCATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                      <  2:213187‑M1/130‑1 (MQ=255)                                                tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                      >  2:167848‑M1/12‑141 (MQ=255)                                                     aggtttATTTAAAGTGATATAATAAACTTAATATAACAAAAACCCTGTTGACTGGTAATAGATGGAGTATGATAAGAAAACGTTTAGAAACTGTTTATTTATATAAGTTTCGATACTTGTATTATATAATGAATAATATCt                                                                                 >  1:264014‑M1/7‑141 (MQ=255)                                                     aagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCt                                                                                 >  2:15669‑M1/7‑141 (MQ=255)                                                       gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTa                                                                               >  1:180445‑M1/5‑141 (MQ=255)                                                       gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTa                                                                               <  1:50486‑M1/137‑1 (MQ=255)                                                       gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTa                                                                               >  1:9108‑M1/5‑141 (MQ=255)                                                        tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAg                                                                              >  1:70584‑M1/4‑141 (MQ=255)                                                                aaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGGTACTTGTATTATATAATGAATAATATCTTAGTGCAAGCa                                                                      >  1:105417/1‑141 (MQ=255)                                                                 aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                     >  1:27599/1‑141 (MQ=255)                                                                 aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                     >  2:289998/1‑141 (MQ=255)                                                                  gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                    <  1:206490/141‑1 (MQ=255)                                                                  gTGCTATAATAAACTTAATATAACAAAAACACCAACTACTGGTAATAGTCGTGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                    <  2:70010/141‑1 (MQ=255)                                                                         aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                             >  2:146659/1‑141 (MQ=255)                                                                         aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                             >  2:140522/1‑141 (MQ=255)                                                                               cTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaat                                                       <  1:15505/141‑1 (MQ=255)                                                                                ttAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaata                                                      >  1:210480/1‑141 (MQ=255)                                                                                 tAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataa                                                     >  1:211897/1‑141 (MQ=255)                                                                                 tAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataa                                                     <  1:289165/141‑1 (MQ=255)                                                                                 tAATATAACAAAAACCACAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataa                                                     <  2:132663/141‑1 (MQ=255)                                                                                  aaTATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataaa                                                    >  2:249513/1‑141 (MQ=255)                                                                                      taACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGAAATCACTTAAATCATTAACTAAATGTa                                                >  2:116077/1‑141 (MQ=255)                                                                                       aaCAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaa                                               >  1:80861/1‑141 (MQ=255)                                                                                          aaaaaCGCCTAGTACTGATAAATTTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATAATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGc                                            <  2:177535/141‑1 (MQ=255)                                                                                            aaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCta                                          <  1:190108/141‑1 (MQ=255)                                                                                             aaCCCCAACTACTGGTAATAGTCGGGGTTTGCTAATAAAACGTTTAGAAACTGTTTATTTAGATAATTTTCGATACTTGTAACACACTATATAAAAAGTCTTGGTGCGATTGTTGAAATCATTAAATAAATGTAAAGCtaa                                         >  1:292558/1‑141 (MQ=255)                                                                                                cccAACTACTTGTAATAGTCGGTGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                      <  1:32756/141‑1 (MQ=255)                                                                                                cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTACTTCAAGCACCTAAATCCTTAAATAAATGTAAAGCtaaata                                      >  2:146154/1‑141 (MQ=255)                                                                                                cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATATTAGTGCAAGCACCTAAATCATTAAATAAATCTAAACCtaaata                                      >  2:130549/1‑141 (MQ=255)                                                                                                 ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATGTAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                     >  1:102753/1‑141 (MQ=255)                                                                                                 ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                     >  1:225141/1‑141 (MQ=255)                                                                                                                 gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTATTATTACct                     >  2:100957/1‑141 (MQ=255)                                                                                                                       gTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAcc               <  1:250659/141‑1 (MQ=255)                                                                                                                          tGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAACCACCTAAATCATTAAATAAATGTAAAGCTCAATAAAAACGTTTTATTACCTCTTACCaaa            >  2:196721/1‑141 (MQ=255)                                                                                                                           ggTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAAc           >  1:253958/1‑141 (MQ=255)                                                                                                                                  aaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAata    <  1:268488/141‑1 (MQ=255)                                                                                                                                  aaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTACAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAata    <  1:148773/141‑1 (MQ=255)                                                                                                                                    aCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATATTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATg  >  1:280999/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                      ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATG  >  CP000731/1‑214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑