New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	512620 =	58 (0.930)	58 (1.350) +TTATCTTGTCCATTTTATTTTTTAACCAAAATTTGATTAA	42/178	NT	59.3%	intergenic (+688/‑6075)	recR/speA_2	Recombination protein RecR/Arginine decarboxylase
	?	USA300TCH1516_ALE	1998278 =	NA (NA)					intergenic (‑6/+3329)	USA300TCH1516_01882/USA300TCH1516_01883	tRNA‑Val/tRNA‑Ala

TGATTACAAGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATGAATGTAATTTATA  >  USA300TCH1516_ALE/1998140‑1998417                                                                                                                                           |                                                                                                                                            tGATTACAAGTCAGTTGCGCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAATGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTaa                                                                                                                                            <  1:727446/140‑1 (MQ=255)  gATTACAAGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTaaa                                                                                                                                           <  1:371876/140‑1 (MQ=37)  gATTACAAGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGGAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTaaa                                                                                                                                           <  1:8238/140‑1 (MQ=37)       cAAGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGCGTTCGATCCGCTGACCCTCTGCGTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGc                                                                                                                                      >  1:105520/1‑140 (MQ=255)         aGTCAGTTGCTCTACCGATGGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCt                                                                                                                                    <  1:167827/140‑1 (MQ=255)         aGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCt                                                                                                                                    <  1:463945/140‑1 (MQ=255)         aGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCt                                                                                                                                    >  1:381312/1‑140 (MQ=255)          gTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGCCGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGCGCTAATTCTCCGATTTCAAACCGCCTg                                                                                                                                   >  2:90894/1‑140 (MQ=255)          gTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTACTTCTCCGACTTAAAactgactg                                                                                                                                   >  2:215028/1‑140 (MQ=255)            cAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCCAATTCTCCGATTTAAAACTGCCTGGc                                                                                                                                 >  2:309239/1‑140 (MQ=255)                 gCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGt                                                                                                                            >  1:193803/1‑140 (MQ=255)                   tctACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATACTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTc                                                                                                                          <  2:381312/140‑1 (MQ=255)                    ctACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTGAAAACTGCCTGGCAACGATCt                                                                                                                         >  1:428862/1‑140 (MQ=255)                      aCCAATTCAGGTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCACCGACCCTCTGCTTGTAAGGTAGATTCTCTCCTAGCTGAGCTAATTCTGCGATTTAAAACTGCCTGGCAACGTTCTAc                                                                                                                       <  1:496597/140‑1 (MQ=17)                       caaaTTGAGCTAGGCCGGCAATATGTAAGAATAAATAGTGGAGAATGAAGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTAct                                                                                                                      >  1:342760/3‑140 (MQ=255)                                       ggCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACACTCTGCTTGTAAAGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTaaat                                                                                                      >  2:99093/1‑138 (MQ=255)                                         cAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTc                                                                                                    >  1:153748/1‑140 (MQ=255)                                               ggaaGAATACATGGTGGAGCATGACGGGTTCGAACCTCCGGCCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTAc                                                                                              <  2:335913/138‑1 (MQ=17)                                                      tAATTGGTGGAGATTGACGGGTTCGCACCGCCGACCTGCTTCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCGCCGATTCAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGAc                                                                                       <  2:512383/140‑1 (MQ=14)                                                       ctatcgtggAGAATGCCGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACg                                                                                      <  1:599970/135‑1 (MQ=255)                                                       aggtggtgcAGAACGACGGGGTCGAACCGCCGACCCTCTGCGTGTAAGGCAGATGCTCGCCCAGCTGAGCTAATGCGCCGATTTAAAACTGCCAGGAACAGAGCAACTCTAGCGGAACGTAAGTTCGACTACCATCGACg                                                                                      <  2:544241/137‑1 (MQ=14)                                                        aaTGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCACCGgcgc                                                                                     >  1:122937/1‑140 (MQ=255)                                                         atcgtggCGAATGACGGGTTCTAACAGCCGACCCTCTGCGTGTGAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGTCGCt                                                                                    <  2:419485/137‑1 (MQ=14)                                                                agaATGACGGGTTCGAACCGCCGACCCTCTCCTACAACCGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGc                                                                             <  1:590602/140‑1 (MQ=17)                                                                 ggaTGACGGGTTCGAACCGCCGACCCTCTGCTTGTCAGGCAGATGCGCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCt                                                                            <  2:432436/138‑1 (MQ=255)                                                                           ttCGAACCGCCTACCCTCTGCTTGTAAGGCAGATGCTCTCCCACCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCtgt                                                                  <  2:657741/140‑1 (MQ=255)                                                                            tCGAACCGCCGACCCTCTGCGTGTAAGGCAGATGCGCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCtgtg                                                                 <  1:323031/140‑1 (MQ=255)                                                                                   gccgACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCa                                                          >  1:597516/1‑140 (MQ=255)                                                                                    ccgACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTGCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCAt                                                         <  2:559608/140‑1 (MQ=255)                                                                                              gCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTACGGAGCTTCACTTCTGTGTTCGGCATGGGGACAgct                                               >  1:460546/1‑138 (MQ=255)                                                                                                 tGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCCCCGGCGCTTAACTTCTGTGTTCGGCATGGGAACAGGtgtg                                            <  1:598649/140‑1 (MQ=255)                                                                                                 tGTAAGGCAGATGCTCTCCCAGCTGAGCGAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTGCTCTAGCGGAACGGAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGtgtg                                            <  1:154051/140‑1 (MQ=255)                                                                                                  gcaaGGCCGACGCTCTCCCAGCTGAGCTAATTCGCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGa                                           <  2:533134/138‑1 (MQ=255)                                                                                                   tAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGGACAGATGTGAc                                          >  2:68610/1‑140 (MQ=255)                                                                                                   tAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGAc                                          >  1:40901/1‑140 (MQ=255)                                                                                                    aaGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGAcc                                         <  1:645384/140‑1 (MQ=255)                                                                                                    aaGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCAGCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGAcc                                         <  2:153748/140‑1 (MQ=255)                                                                                                     aGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGAcct                                        <  1:364341/140‑1 (MQ=255)                                                                                                      ggCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGCACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGAcctc                                       <  1:389491/140‑1 (MQ=255)                                                                                                           agtctcgCCCAGCTGAGCTTAGTCCCCGATTTAAAACTGCCGGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGc                                  <  2:428862/137‑1 (MQ=14)                                                                                                           aTGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCttac                                  >  2:668274/1‑138 (MQ=255)                                                                                                           aTGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGc                                  <  2:105520/140‑1 (MQ=255)                                                                                                           aTGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGAATACCATCGACGCTAAGGCGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTACTTGc                                  >  2:21832/1‑140 (MQ=255)                                                                                                               tctgCCAGCGGGGCTAAGTCTCCGATTTAAAACGGCGTGGCAACGTTCTACGCTAGCGGAACGTAAGTTCGACGACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCtata                              <  1:124599/140‑1 (MQ=14)                                                                                                                ctcCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAg                             >  1:84264/1‑140 (MQ=255)                                                                                                                 tcCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGt                            <  1:275407/140‑1 (MQ=255)                                                                                                                  cccAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGCCGCTAAGGCGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTACTATAGta                           >  1:183202/1‑139 (MQ=255)                                                                                                                  cccAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTc                           <  2:595783/140‑1 (MQ=255)                                                                                                                    cAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCCAAGGAGCTTAACTTCTGTGTTCGGCATGGGACCAGGTGTGCCCTCCTGGCTATATTccc                         >  1:38987/1‑138 (MQ=14)                                                                                                                        tcaGCTAACCCTCCGATTTAAACCTGCCTGGCAACGTTCTACTCTATCGGACCGTAAGTTCGACTACCTCCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGa                     <  1:387382/138‑1 (MQ=14)                                                                                                                        tGAGCTAATTCTCCGATTGAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGa                     <  1:710886/140‑1 (MQ=255)                                                                                                                          aGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACa                   <  1:528013/140‑1 (MQ=255)                                                                                                                            cTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACata                 >  1:24710/1‑140 (MQ=255)                                                                                                                              aaTTCTCCGATTTAAAGCGTCCTGGCAACGGTCTACTCGAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTACTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATg               <  2:193803/140‑1 (MQ=14)                                                                                                                              aaTTCTCCGATTTAAAACTGCCTGGCAGCGTGCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATg               <  2:373123/140‑1 (MQ=255)                                                                                                                                    tcattagaaatCTGCCTCGCAACTTTCTACTCTCGCGCAACGTAAGTTCGACTACCATCGACGCTACGTAGCTTACCTCCTGTGCTCCGCATGGGATCAGTTGTGACCTCCTTGCTATAGTCACCAGACATATGAATGTa         <  2:55615/133‑1 (MQ=11)                                                                                                                                    ccGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATGAATGTa         <  1:588318/140‑1 (MQ=40)                                                                                                                                          tAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTGACCAGACATATGAAGGTAATTtat   >  2:264803/1‑140 (MQ=21)                                                                                                                                          tAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATGAAGGTAAGTtat   >  2:218191/1‑140 (MQ=21)                                                                                                                                           aaaaCTGCCTGGCAATGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACGTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATGAATGTAATTtata  <  2:357367/140‑1 (MQ=18)                                                                                                                                           |                                                                                                                                            TGATTACAAGTCAGTTGCTCTACCAATTGAGCTAGGCCGGCAATATGTAAGAATAAATGGTGGAGAATGACGGGTTCGAACCGCCGACCCTCTGCTTGTAAGGCAGATGCTCTCCCAGCTGAGCTAATTCTCCGATTTAAAACTGCCTGGCAACGTTCTACTCTAGCGGAACGTAAGTTCGACTACCATCGACGCTAAGGAGCTTAACTTCTGTGTTCGGCATGGGAACAGGTGTGACCTCCTTGCTATAGTCACCAGACATATGAATGTAATTTATA  >  USA300TCH1516_ALE/1998140‑1998417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.