Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,567 T→C 13.0% D1223D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5670TC13.0% 132.9 / 4.5 72D1223D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (15/44);  new base C (8/2);  total (25/47)
Fisher's exact test for biased strand distribution p-value = 1.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAC  >  USA300TCH1516_ALE/618430‑618705
                                                                                                                                         |                                                                                                                                          
cctgatacagattcagacaatgactctgattcagacagcgactCAGACTCAGATAGTGATTAAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcatattc                                                                                                                                          <  2:43845/133‑1 (MQ=14)
agcgactcagattcagacagagactcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                                                                                                          <  1:423808/140‑1 (MQ=255)
       cagattcagacagcgactcagattcagacagcgactCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGc                                                                                                                                   >  1:313675/1‑140 (MQ=255)
       cagattcagacagcgactcagattcagacagcgactCAGACTCAGATAGTGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGc                                                                                                                                   <  1:476990/140‑1 (MQ=255)
           ttcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACGCAGACTCAGATAGCGACTCGGACTCAGACAGCGACt                                                                                                                               >  2:166181/1‑140 (MQ=14)
            tcaaccagcgacacagattcagccagcgcctcagaCTCAGATAGTTAGTCAGATTCAGATAGCGACGCAGATTCAGACAGCTACTCAGATTCAGACAGCGCCTCAGACTCATATAGTGACTCAGATTCAGATAGCGACTc                                                                                                                              <  1:8010/135‑1 (MQ=14)
               gacagcgagtgcgattccgacaccggctcagaCTCAGATAGTGAATCCGAAACGGTTGTCTACTCCGCTTCTGACATCGACGCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGa                                                                                                                           <  1:143498/140‑1 (MQ=9)
               gacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATACCGACACAGa                                                                                                                           >  2:306732/1‑140 (MQ=11)
                 cagcgactcagattcagacagcgactcagaCTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACGCAGact                                                                                                                         >  2:711617/1‑138 (MQ=11)
                   gcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCg                                                                                                                       <  1:144748/140‑1 (MQ=255)
                   gcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCg                                                                                                                       <  1:180432/140‑1 (MQ=255)
                     gactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                                     <  1:606225/140‑1 (MQ=255)
                       ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACCCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGGCTCAGATTCGGCCa                                                                                                                   >  2:370785/1‑140 (MQ=17)
                        tcctatgcagacagcgagtcggaCTCGGACGAGGAGTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACATCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                  <  2:703600/133‑1 (MQ=11)
                        tcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                  >  1:466916/1‑140 (MQ=255)
                        tcagattcagacagcgactcagaCTCAGATAGAGATTCAGACTCAGCGAGCGACTCAGATTCAGCGAGCGAATCAGACTCAGAGAGCGAATCAGACTGAGAGAGAGACCAAGAATCAGAGAGAGACTCAGACTCagagag                                                                                                                  >  2:168801/1‑140 (MQ=2)
                          ggattcagacagcgactcagaCTCAGGTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCg                                                                                                                <  1:738287/139‑1 (MQ=255)
                           gactcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                               <  1:218378/137‑1 (MQ=255)
                              tcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGCCTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                                                            <  1:480233/140‑1 (MQ=255)
                                 aagaccgacacagaCTCAGACAGCGATACAGACTCAGATAGCGACTCCGATTCAGACACCGACTCAGACTCAGACAGCGACTCAGACTCCGACAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                         <  2:315947/135‑1 (MQ=14)
                                     gcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCATACAGCGCAACAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGTTGCAGATAGCGACTCTGATTCGGGCGGCGATGACGACTCa                                                                                                     >  2:272918/1‑140 (MQ=14)
                                      cgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                                                                    >  1:381628/1‑140 (MQ=255)
                                        actcagactcagATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGCCTCAGACTCAGATAGCGATGCAGACTCAGAt                                                                                                  >  2:748406/1‑140 (MQ=11)
                                         ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATa                                                                                                 >  1:163649/1‑140 (MQ=255)
                                          tcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCGGATTCAGATAGCGACTCAGACTCGGAGAGGGACTCAGACTCGGagag                                                                                                >  2:435276/1‑140 (MQ=14)
                                              actcagaTAGAGATTGAGATTCAGATAGCGACTCAGATTCAGACAGCGACTGAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                            <  2:476438/140‑1 (MQ=255)
                                                         gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                 >  1:298039/1‑140 (MQ=255)
                                                         gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACGCAGATAGCGACTCAGACTCAGATAGCGACTCAGACACGGACAGCGAAGCAGACTCAGATAGCGACtcagatgcaga                                                                                 >  2:398149/1‑140 (MQ=14)
                                                            tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                              <  1:79856/140‑1 (MQ=255)
                                                             cAGACTCAGATGGCGACTCAGATCCAGACAGCGATTCAGACTCAGACATGGACGCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc                                                                             <  2:684180/140‑1 (MQ=14)
                                                             cAGACTCAGATAGAGACACAGATTCAGACAGCGACTCAGACTCAGACAGCGACACAGACTCAGACAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc                                                                             <  2:441940/140‑1 (MQ=255)
                                                              agtcTCAGCTTGAGACGCATATTCTGACAGCGATACAGACTAAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                            <  2:692884/137‑1 (MQ=14)
                                                              aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                            <  1:548103/140‑1 (MQ=255)
                                                               gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                           <  1:81005/140‑1 (MQ=255)
                                                                aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                          <  1:90147/140‑1 (MQ=255)
                                                                 cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAATAAGACTCAGATAGCGACTCAGAATCAGCTAgagaga                                                                         >  2:208325/1‑138 (MQ=11)
                                                                  tCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                  <  1:404793/98‑1 (MQ=255)
                                                                  tCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAg                                                                                                                  >  2:404793/1‑98 (MQ=255)
                                                                     gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                     >  1:644102/1‑140 (MQ=255)
                                                                     gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                     <  1:8335/140‑1 (MQ=255)
                                                                      aTAGCGACTCAGATTCAGCCAGCGACTCAGACTCAGACGGCGCCTCAGACAAAAACACACACGCAGTCGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                    <  1:175224/140‑1 (MQ=14)
                                                                      aTAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                    <  1:622154/140‑1 (MQ=255)
                                                                         gtgACGCAGAAACAGACAAGGACGCAGACTCAGACAGCGAGACAGACACAGATAGTGACTCAGATTCAGATAGGGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                 <  2:50737/138‑1 (MQ=14)
                                                                         gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                 <  1:663877/140‑1 (MQ=255)
                                                                         gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                 <  1:645434/140‑1 (MQ=255)
                                                                          cGACTGCGACTCACAAAGCGACGCAGACTCAGACAGCGACTCAGTCTCCAAGATCTACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                <  2:656660/140‑1 (MQ=11)
                                                                           gagtCAGAGTAAGACACAGATTCAGATTCAGACAGCGACACAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                               <  2:563807/137‑1 (MQ=14)
                                                                              ccAGACTCAGCCAGCGACACAGACTCAGATAGCCACTCAGACTCACATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                            <  2:104823/139‑1 (MQ=14)
                                                                               cAGATTCAGACAGCGACTCAGACTCAGACACCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGATTCGGACTCAGATAGc                                                           <  1:449770/140‑1 (MQ=255)
                                                                                 gATGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                         <  1:417233/140‑1 (MQ=255)
                                                                                  aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                        <  1:528934/140‑1 (MQ=255)
                                                                                  aTTCAGACACCGACACAGACTCAGACAGCCACTCAGACTCAGATAGTGACGCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                        <  2:697469/140‑1 (MQ=255)
                                                                                    tcggacagcgcgtcgcacacagacagcgactcagagtcgGATAGTGACTCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                      <  2:298556/137‑1 (MQ=14)
                                                                                    tcagagagcgagagacactcagagagcgatacagacTCAGATACTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                      <  2:191830/140‑1 (MQ=14)
                                                                                      ggacggccactcatactcatacacggactctgactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                    <  2:689602/139‑1 (MQ=14)
                                                                                      agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                    <  1:463135/140‑1 (MQ=255)
                                                                                       gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGCCTCAGACAGCGACtcaga                                                   >  1:148700/1‑140 (MQ=255)
                                                                                        acagcgactcagactcagacagcgactcagactcagATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                  <  1:19341/140‑1 (MQ=255)
                                                                                           gcgactcagactcagacagcgactcagactcagaTCGTGACTCAGAGACAGATAGCGACTGAGAGTCGGACAGCGATCGAGACGCAGATAGCGACTCAGATTCCGATAGAGATACGAACTCCGAGAGCGCAgcagattca                                               >  2:22767/1‑140 (MQ=14)
                                                                                             gactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                             <  1:370785/140‑1 (MQ=255)
                                                                                                 cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGt                                         >  1:466416/1‑140 (MQ=255)
                                                                                                   gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                       <  1:95838/140‑1 (MQ=255)
                                                                                                     ctcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                     <  1:711617/140‑1 (MQ=255)
                                                                                                         gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCCGATAGCGACTCAGATTCAGATAGTGATTCAGa                                 >  1:309892/1‑140 (MQ=255)
                                                                                                         gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                 <  1:423104/140‑1 (MQ=255)
                                                                                                             gcgacgcagactcagaTAGTGACCAAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                             <  1:236192/140‑1 (MQ=255)
                                                                                                              cgactcaggctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATCCGGAATCAGTGCGGGACTCTGATTTAGATAGTGATTCAGACTCAg                            <  1:326929/140‑1 (MQ=17)
                                                                                                                 ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                         >  1:466833/1‑140 (MQ=255)
                                                                                                                    agactcagaTAGTGACTCAGATTCAGATAGCCACTCAGCCGCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg                      <  1:320530/140‑1 (MQ=17)
                                                                                                                    agacgcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg                      <  1:689516/140‑1 (MQ=255)
                                                                                                                     gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGa                     <  1:510868/140‑1 (MQ=255)
                                                                                                                               gTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATCGCGCCTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGAGATTCAGACTCAGATAGCGACACAGACTCa           >  2:343434/1‑140 (MQ=14)
                                                                                                                                 gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATACCGACTCGCACTCAGATCGCGACTCAGATTCAGATAGTGAATCATCCCCAGATCGCGGCTCAgcgcacgc         >  2:163565/1‑133 (MQ=14)
                                                                                                                                   cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCCGATAGTGATTCAGACTCAGATAGCGACTCAGCTTCAGACa       >  1:204310/1‑140 (MQ=255)
                                                                                                                                    tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCCGATAGCGACCCAGAATCAGACAg      >  2:482062/1‑140 (MQ=14)
                                                                                                                                        attcagatAGCGACGCAGATGCAGACAGCGATTCAGACACAGACAGCGACTCAGATTCAGAAAGCGACTCGGACTCAGAAAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc  <  2:466916/140‑1 (MQ=11)
                                                                                                                                         |                                                                                                                                          
AGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAC  >  USA300TCH1516_ALE/618430‑618705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: