Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,744,446 G→T 9.7% P76T (CCT→ACT)  apt ← Adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,744,4460GT 9.7% 168.3 / 5.9 67P76T (CCT→ACT) aptAdenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (34/26);  new base T (3/4);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

AATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTC  >  USA300TCH1516_ALE/1744314‑1744581
                                                                                                                                    |                                                                                                                                       
aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                                                                                                                  <  1:601449/140‑1 (MQ=255)
 atcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGc                                                                                                                                 <  2:107852/140‑1 (MQ=255)
    atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCaa                                                                                                                              <  1:79/140‑1 (MQ=255)
     tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCGTATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTTCAGCAGGTGCAAAACCAAt                                                                                                                             >  2:524925/1‑140 (MQ=255)
      cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATc                                                                                                                            <  1:584572/140‑1 (MQ=255)
      cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTCCTTTTCTAACAGGTGCAAAGCCAATc                                                                                                                            >  2:637496/1‑140 (MQ=255)
               acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGaa                                                                                                                   <  1:79528/140‑1 (MQ=255)
               acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                                                                                                   <  1:731791/140‑1 (MQ=255)
                 acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTATATATCAGACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATa                                                                                                                 >  2:231388/1‑140 (MQ=255)
                   acGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAg                                                                                                               >  1:537241/1‑140 (MQ=255)
                        gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACTGTTGCAAAGCCAATCCCCATTGAATAAGCTACa                                                                                                          >  2:132350/1‑140 (MQ=255)
                          ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAgg                                                                                                        <  2:599909/140‑1 (MQ=255)
                           cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTCCACGAAGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGa                                                                                                       <  1:402878/140‑1 (MQ=255)
                                 tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCa                                                                                                 <  2:507927/140‑1 (MQ=255)
                                    ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGAATTCACGAGGTAGTCTTCCACCCCATCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATg                                                                                              >  2:540974/1‑140 (MQ=255)
                                    ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATAATCATAACGAATGACTTCACGAGGTGATTTCACTTCTTTTCTAACAGGTGCAAAGCCAATCACCATTGAATAAGCTACAGGACAGCCAATg                                                                                              >  2:383390/1‑140 (MQ=255)
                                     tGCATCTTTGTGCATTGTTAGAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATTCTCATTGAATAAGCTACAGGACAGCCAATGa                                                                                             <  2:345579/140‑1 (MQ=255)
                                               tacATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcg                                                                                   >  2:179581/3‑140 (MQ=255)
                                                 gaTTGTTAAAACATGTGTACCATATGCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcgcg                                                                                 <  1:302344/139‑1 (MQ=255)
                                                 cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcgcg                                                                                 <  2:731338/140‑1 (MQ=255)
                                                 cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAACAAGCTACAGGACAGCCAATGATAAAGCCAcgcg                                                                                 <  2:363245/140‑1 (MQ=255)
                                                  aTTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                <  1:544314/140‑1 (MQ=255)
                                                  aTTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                <  1:382642/140‑1 (MQ=255)
                                                  aTTGTTAAAACATTCGTACCATATTCTAGGTCACACTCATATCGAATGACTCCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAACGCCACgcgc                                                                                <  2:593766/140‑1 (MQ=255)
                                                   ttGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCt                                                                               >  1:424954/1‑140 (MQ=255)
                                                     gTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTc                                                                             >  1:652679/1‑140 (MQ=255)
                                                       tAAAACATTTGTCCCAGATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                           <  2:2092/140‑1 (MQ=255)
                                                          aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAACGCCAATCCCCCTTGAATAAGCTACAGGACAGCCAATGATAAACCCACCCGCTTCAGCTc                                                                        >  2:669207/1‑140 (MQ=255)
                                                           aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAATCCATTCCCCCTTGAATAAGCTACAGGACAGCCAATGATCCAGCCGCGCGCTTCAGGTcc                                                                       >  1:218440/1‑140 (MQ=255)
                                                           aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                       >  1:539876/1‑140 (MQ=255)
                                                           aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                       >  1:743030/1‑140 (MQ=255)
                                                             aTTTTTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAATGCCTATCCCCATTGAATATGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTa                                                                     >  1:664473/1‑140 (MQ=255)
                                                               ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAAGCGCTTCCGGTCCGACa                                                                   >  2:252788/1‑140 (MQ=255)
                                                                 gaaCCATATTCTCGGTCATACACATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAAc                                                                 <  1:121667/138‑1 (MQ=255)
                                                                  tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                                >  1:153602/1‑140 (MQ=255)
                                                                     cATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata                                                             <  1:325797/140‑1 (MQ=255)
                                                                      atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAACGCCACGCGCTTCAGGTCCTACAACGatat                                                            >  1:492532/1‑140 (MQ=255)
                                                                      atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCCGGTCCTACAACGatat                                                            >  1:748383/1‑140 (MQ=255)
                                                                      agatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGatat                                                            <  2:31201/138‑1 (MQ=255)
                                                                          tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCACTTCTTTTGTAACGGGTGCAAAGCCAATCCCCATTGAATAAACTGCAGCACACACAATGCGACAGCCAGCCGCGACAGGACCTCCAAGGaaaagaga                                                        >  2:722390/1‑133 (MQ=255)
                                                                           cgaGGTCATACTCATAACGAATGACGTCACGAGGTAATTGCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa                                                       <  2:399354/138‑1 (MQ=255)
                                                                              ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                    >  1:28658/1‑140 (MQ=255)
                                                                               gTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                   <  1:685646/140‑1 (MQ=255)
                                                                                tCATACGCAGAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATctct                                                  <  1:524925/140‑1 (MQ=255)
                                                                                     cTCATAACGAATGACTTCACTAGGTAATTTCCCTTCTTTTCTAACAGGAGCAAAGCCAGTCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCGACCACGATATCAACCTCCCCGTCtc                                             >  2:217508/1‑139 (MQ=255)
                                                                                     cTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAAGATAAAGCAACGCGCTTGAGGTCCTACAACGACATCAAAATCTCTGTCtt                                             >  2:192014/1‑140 (MQ=255)
                                                                                        aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATGAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtttta                                          >  2:405763/1‑139 (MQ=255)
                                                                                               aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTCCGTATTc                                   >  1:475108/1‑140 (MQ=255)
                                                                                               aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAAAGATATCAACGTTTCTAGCTTTTGGGTATTc                                   >  1:714989/1‑140 (MQ=255)
                                                                                               aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAT‑CCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCTGGTCCTACAACGATACCCACCTCTCTAACTTTTTCGTTTTCt                                  >  1:674506/1‑140 (MQ=255)
                                                                                                tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCCATGATAAAGCCACGCGCTTCAGGTCCTACAACGACATCAACATCTCTGTCTTTTGCGTATTCt                                  >  2:638452/1‑140 (MQ=255)
                                                                                                  aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACGTCTCTGTCTTCTGCGTATTCttc                                >  1:98181/1‑138 (MQ=255)
                                                                                                   cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                               >  1:623344/1‑140 (MQ=255)
                                                                                                     tCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAACCACCATAGAATAAGCTAAAGGACTGCCAATTATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAt                             <  1:286045/140‑1 (MQ=255)
                                                                                                       aCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAGGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAACGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                           <  2:472958/140‑1 (MQ=255)
                                                                                                             tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                     <  2:424954/140‑1 (MQ=255)
                                                                                                             tAATTTCCCTTCTTTTCTAACACGTGCAAAGCCTATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                     <  2:569576/140‑1 (MQ=255)
                                                                                                              gaTTTCCCCTCTGTGCTAACGGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCCTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTATAATTTTATCTGt                    <  2:668855/139‑1 (MQ=255)
                                                                                                              aaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGCCAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTATATCTGt                    >  2:25056/1‑140 (MQ=255)
                                                                                                               aTTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                   <  1:315331/140‑1 (MQ=255)
                                                                                                                     cttcttTTCTAACAGGTGCAAAGCCAATCCCCATTGCATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGTTACTACAACGATATCAGCATCTCTGTCTTTTCCGTCTTCTACAATTTTATCTGTTCCAtcg             >  2:436617/1‑138 (MQ=255)
                                                                                                                          tttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTCTATCTGTTGCACAGCCata        >  2:693520/1‑140 (MQ=255)
                                                                                                                             cTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCATGCGCTTCAGGTCCTACAACGATATCCACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGc     >  1:303747/1‑140 (MQ=255)
                                                                                                                              tAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCt    >  1:313723/1‑140 (MQ=255)
                                                                                                                               aaCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACACTTTTATCTGTTCCATAGCCATATCCtt   >  1:529445/1‑140 (MQ=255)
                                                                                                                               aaCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCtt   >  1:134312/1‑140 (MQ=255)
                                                                                                                                aCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc  <  1:437641/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
AATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTC  >  USA300TCH1516_ALE/1744314‑1744581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑