Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA98.2% 194.5 / ‑6.0 68A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/31);  minor base T (1/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTG  >  USA300TCH1516_ALE/2279575‑2279840
                                                                                                                                    |                                                                                                                                        
gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCCTCAGTTGCTGATGTTACTGTTGttt                                                                                                                                 >  1:75366/1‑140 (MQ=255)
 ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                                >  1:72630/1‑140 (MQ=255)
        ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCGTCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                         >  1:202333/1‑140 (MQ=255)
         tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                        <  1:260598/140‑1 (MQ=255)
           aTTCTTCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGcc                                                                                                                      <  2:447219/140‑1 (MQ=255)
           aTTCTACAACTGATTTACTGGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGcc                                                                                                                      <  1:472927/140‑1 (MQ=255)
           aTTCCACTTCTGGTTTACCAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGcc                                                                                                                      <  1:600867/140‑1 (MQ=255)
                 cTTCTGGTTTACTAGTTACATCGTTATCCCTTGTCGGACTGTTTGTTGATGCATCTACACTAGAAGTGTTATTAGCTTGC‑GGTTTATC‑GTTTGCATCATCAGTCGCTGATGTTACTGCTGTTTCCCCCGTTGCCGCATCa                                                                                                                >  1:221247/1‑140 (MQ=255)
                 cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCTTCa                                                                                                                >  1:452370/1‑140 (MQ=255)
                  ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  1:14377/140‑1 (MQ=255)
                     tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta‑t                                                                                                           <  2:249078/140‑1 (MQ=255)
                     tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta‑t                                                                                                           >  2:642573/1‑140 (MQ=255)
                          tACTAGTTACATCGTTATCCATTGTCGGCCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGGTGTTCCTGTTGTTTCACCTGTTCCCGCCTCACTA‑TTAttt                                                                                                      >  2:247460/1‑140 (MQ=255)
                          tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTAttt                                                                                                      <  1:374124/140‑1 (MQ=255)
                           cctggTTACCTCTTTATCCATTTTCGGACTGTTTGTTGATGCTTCTACACTAGATTTGTTATTAGCTTGC‑GGTTTTTC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTg                                                                                                     <  2:713086/136‑1 (MQ=255)
                            cTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTgg                                                                                                    <  1:69136/140‑1 (MQ=255)
                             tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTCGCGGGCTCTTCTATCTGCTT‑‑TTACTGGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGgt                                                                                                   >  2:162535/1‑140 (MQ=255)
                              tgTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCGCCTGTTGCCGCATCACTA‑TTATTTGgtg                                                                                                  <  2:268523/139‑1 (MQ=255)
                              aGTTACATGTTTATCCATTGGTGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTTC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGgtg                                                                                                  <  2:155233/140‑1 (MQ=255)
                                      cGTTATCCATTGTCGGACTGTTTGTTTCTGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGga                                                                                          >  2:398356/1‑140 (MQ=255)
                                      cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGga                                                                                          <  1:211879/140‑1 (MQ=255)
                                        ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGgagc                                                                                        >  1:122336/1‑139 (MQ=255)
                                           tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGc                                                                                     <  1:571842/140‑1 (MQ=255)
                                            ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCg                                                                                    >  1:516406/1‑140 (MQ=255)
                                             cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCCA‑TTATTGCGTGTTGTCGGAGCAGCGt                                                                                   >  2:44856/1‑140 (MQ=255)
                                             cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGCAGCGt                                                                                   >  1:740274/1‑140 (MQ=255)
                                              aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTc                                                                                  >  1:599804/1‑140 (MQ=255)
                                              aTTGTCGGACTGTTTGTTGATGCATCTACAATATAATTTTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTc                                                                                  <  1:516747/140‑1 (MQ=255)
                                               ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTCTTTGGTGTTGTCGGAGAAGCGTCt                                                                                 >  1:456903/1‑140 (MQ=255)
                                               ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCt                                                                                 >  1:581483/1‑140 (MQ=255)
                                                tGTCGGACTGTTTGTTGATGCATCTACACTATAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                <  1:474466/140‑1 (MQ=255)
                                                   cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTAAAGTGTTTGAATTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGATGTCGGCGACGCCTcttctt                                                                             >  1:555268/1‑140 (MQ=255)
                                                     gtttgtgtgtcGATGCTTCTACACTAGGTTTGTTATTAGCTTGC‑TGTTTATC‑CTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                           <  2:151687/137‑1 (MQ=255)
                                                          tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAtt                                                                      >  1:24049/1‑140 (MQ=255)
                                                          tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAtt                                                                      >  1:24546/1‑140 (MQ=255)
                                                                 aTGCATCTACACTAGAATTCTTATTAGCTTGC‑GATTTTTC‑ATTTGAATCATCAGTTGCTGATATTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGAGTTTGTCGGAGATGAGTCTGTATTGACATTATCTgtc                                                               >  2:53325/1‑140 (MQ=255)
                                                                   gCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                             >  1:254994/1‑140 (MQ=255)
                                                                     aTCTACACTCGTATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACGA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct                                                           <  2:582548/140‑1 (MQ=255)
                                                                         ctgcgaGAATTGGTATCAGCTCGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                       <  1:279152/135‑1 (MQ=255)
                                                                         acacTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                       <  1:218414/140‑1 (MQ=255)
                                                                         acacTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAACACGA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                       <  1:481180/140‑1 (MQ=255)
                                                                          cacTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGACTGGCATGCCATTACCTGTAGTCTCAGAt                                                      >  2:367014/1‑140 (MQ=255)
                                                                               gAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                                 <  1:669225/140‑1 (MQ=255)
                                                                               gAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                                 <  1:658248/140‑1 (MQ=255)
                                                                                aaTTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTa                                                >  1:267003/1‑140 (MQ=255)
                                                                                 aTTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAg                                               <  1:354872/140‑1 (MQ=255)
                                                                                      tattaGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGCTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGt                                          >  1:487853/1‑140 (MQ=255)
                                                                                        ttaGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTAACCTGTTGCCGCATGACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTGTGAGATACGATAGGgtgtac                                        >  2:600866/1‑138 (MQ=255)
                                                                                             ttGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAt                                   >  1:571780/1‑140 (MQ=255)
                                                                                                  ggTTTATC‑ATTTGCATCATAAGTTGCTTGTGTTACTGGTGTTTCACCTGTTGCCGCATCACTA‑TTTTATGTGGTTGTCTGAGAAGCTTCTGCGTTGCCATTAGCTGTCGTTTCAGTGATGTTAGGTTGCCCAGTATTTTc                               >  2:231671/1‑140 (MQ=255)
                                                                                                    tttATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                             <  2:24049/140‑1 (MQ=255)
                                                                                                    tttATC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                             <  2:24546/140‑1 (MQ=255)
                                                                                                        tC‑ATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                         <  1:340499/140‑1 (MQ=255)
                                                                                                            tttGCTCCACCAGTTGCTGATGTTACTGTTGTTTCACCTGTTTCCGCATCAGTG‑TGATCTCGTGTTGTAGAAGAAGCGTCTGCTTTGCCATTTGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                      <  2:124706/140‑1 (MQ=255)
                                                                                                                 atcatcAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAg                 <  1:361599/140‑1 (MQ=255)
                                                                                                                  tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGTTGTCCAGTCTTTTCTGGTGTTGCTTTAGc                >  1:252749/1‑140 (MQ=255)
                                                                                                                   catcaGTTGCTGATGTTACTGTTGTTTCACCCGGTGCCGCA‑CACAACTTGCGCAATTTGGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTACGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa               <  1:753388/140‑1 (MQ=255)
                                                                                                                    atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt              <  2:516406/140‑1 (MQ=255)
                                                                                                                    agcaGTTGCTGATGTTCCTGGTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt              <  1:225498/138‑1 (MQ=255)
                                                                                                                       aGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCCGTATTTTCTGGTGCTGCATTGGCACTTg           >  2:248824/1‑140 (MQ=255)
                                                                                                                         ttGCTGTTGTTTCTGGTGTGTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTGGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa         <  2:407834/140‑1 (MQ=255)
                                                                                                                          tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGATAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTGCAGTATTTTCTGGTGTTGCATTAGCATTTGact        >  1:194677/1‑138 (MQ=255)
                                                                                                                           gCTGATGTTACTGTTGTTTCTTCTGTTGCCGCATCGCTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt       <  2:161380/140‑1 (MQ=255)
                                                                                                                              gATTTTTCTGTTTTTTCGCGTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc    <  2:740274/140‑1 (MQ=255)
                                                                                                                              gATGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc    >  1:349179/1‑140 (MQ=255)
                                                                                                                                tGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTGGGTTGTCCAGTATTTTCTGGTGTTGCATTGGCGTTTGAATTTGCTg  >  1:195342/1‑140 (MQ=255)
                                                                                                                                tGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGCATTTGCTg  >  1:318533/1‑140 (MQ=255)
                                                                                                                                tGTTACTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg  >  2:268016/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                        
GTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGC‑GGTTTATC‑ATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTA‑TTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTG  >  USA300TCH1516_ALE/2279575‑2279840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑